Impact of Nematode Infections on Non-specific and Vaccine-Induced Humoral Immunity in Dual-Purpose or Layer-Type Chicken Genotypes.

Nematode infections may induce immune-modulatory effects and influence host-immune responses to other pathogens. The aim of the study was to investigate whether a mixed nematode-infection influences non-specific and vaccine-induced humoral immunity against Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), and Avian Metapneumovirus (AMPV) in already vaccinated hens of a dual-purpose (Lohmann Dual, LD) or a layer genotype (Lohmann Brown Plus; LB). Until 17 weeks-of-age, LD (n = 70) and LB (n = 109) hens were vaccinated against major bacterial and viral diseases and coccidiosis. At 24 weeks-of-age, the hens received either a placebo or an oral inoculation of 1,000 infectious eggs of A. galli and H. gallinarum. Plasma total immunoglobulin (Ig) isotypes (IgY, IgM, IgA) levels and vaccine-induced antibody titers against NDV, IBV, and AMPV were determined from 2 to 18 weeks post-infection (wpi). Infections had no suppressing effect on total Ig isotypes IgY, IgM, and IgA as well as on vaccine-induced antibody titers against NDV, IBV, and AMPV (P > 0.05). Overall, LB hens had higher levels of IgY, IgM, and IgA than those of LD hens (P < 0.05). There were no differences between IBV titers of the two genotypes (P > 0.05). Independent of infection status of the hens, NDV titers were higher in LB hens than in LD hens at wpi 2 (P < 0.05), but not in following weeks (P > 0.05). Uninfected LD hens had lower AMPV titers than their infected counterparts at 6 and 14 wpi (P < 0.05). Regardless of nematode infection, LD hens revealed a higher risk of responding weak to vaccination against NDV (odds ratio = 5.45; P = 0.021) and AMPV (odds ratio = 13.99, P < 0.001) than did LB hens (P > 0.05). We conclude that nematode infections have no adverse effects on non-specific and vaccine-induced humoral immunity in either genotype. LB hens have higher levels of total immunoglobulin isotypes than LD hens. Except for IBV, vaccine-induced humoral immune responses show a dependency on genotype. Dual-purpose hens show lower responsiveness to vaccinations against NDV and AMPV, possibly due to factors associated with increased body fat reserves in this genotype.

Genotype-associated differences in bursal recovery after infectious bursal disease virus (IBDV) inoculation.

T-cell immune responses were shown to play an important role in the regulation of infectious bursal disease virus (IBDV) replication and development of lesions in the bursa of Fabricius (BF) (bursal lesions) but also in the recovery from the infection. Studies suggested that the host-genotype influences T-cell responses during the acute phase of infection. Genotype-related differences in the recovery phase were not investigated so far. The present study used commercial broiler- (BT), layer- (LT), dual-purpose type (DT) chicken lines as well as a specific pathogen free (SPF) LT chicken as a reference for comparison of T-cell related differences in IBDV-immunopathogenesis not only in the early phase post inoculation (pi) but also in the recovery phase. The Deventer formula was used to determine the optimal time point of inoculation with an intermediate plus IBDV strain when maternally derived antibody (MDA) titers were below the calculated breakthrough level of the virus for all genotypes. Differences in the bursal lesion development, intrabursal CD4+ and CD8+ T-cell accumulation and numbers of IBDV-positive cells were determined. In addition, anti-IBDV antibody development and the relative amount of anti-inflammatory cytokine mRNA were recorded until 28 days post IBDV inoculation. Differences between the genotypes were observed in the duration and magnitude of bursal lesions, CD4+ and CD8+ T-cell infiltration as well as the presence of anti-inflammatory Interleukin (IL)-10 and Transforming growth factor (TGF) ß4 cytokine mRNA (P < 0.05). While the investigated immune parameters were comparable between the genotypes at seven days pi, during 14, 21 and 28 days pi a delayed recovery process in LT and DT chickens compared to BT chickens was observed (P < 0.05). Furthermore, the age and residual MDA levels had a genotype-dependent influence on the onset of the anti-IBDV specific humoral and T-cell mediated immune responses. This study suggests, that the impact of T-cell immunity on the recovery process after IBDV infection may need to be considered further for the development of new breeding programs for disease resistant chicken lines.

Immune responses upon in ovo HVT-IBD vaccination vary between different chicken lines.

The genotype of chickens is assumed to be associated with variable immune responses. In this study a modern, moderate performing dual-purpose chicken line (DT) was compared with a high-performing layer-type (LT) as well as a broiler-type (BT) chicken line. One group of each genotype was vaccinated in ovo with a recombinant herpesvirus of turkeys expressing the virus protein VP2 of the infectious bursal disease virus (HVT-IBD) while one group of each genotype was left HVT-IBD unvaccinated (control group). Genotype associated differences in innate and adapted immune responses between the groups were determined over five weeks post hatch. HVT-IBD vaccination significantly enhanced humoral immune responses against subsequently applied live vaccines compared to non-HVT-IBD vaccinated groups at some of the investigated time points (P < 0.05). In addition HVT-IBD vaccination had depending on the genotype a significant impact on splenic macrophage as well as bursal CD4+ T-cell numbers (P < 0.05). On the other hand, the detectable genotype influence on Interferon (IFN) γ and nitric oxide (NO) release of ex vivo stimulated spleen cells was independent of HVT-IBD vaccination. The results of our study suggest considering a genotype specific vaccination regime in the field.

Varying antibody responses of laying hens housed in an aviary system and in furnished cages.

Antibody titers after vaccination against Infectious Bronchitis Virus (IBV), Newcastle Disease Virus (NDV) and after natural infection with Campylobacter were analyzed in five trials with Lohmann Silver laying hens kept in two different housing systems. In these studies it could be demonstrated that antibodies against IBV and Campylobacter were in 4 out of 5 respectively in 2 out of 5 trials significantly higher in hens housed in an aviary system compared to those kept in furnished cages. The opposite trend was observed for antibodies against NDV which were on average significantly higher in cages. The mean mortality rate was slightly higher in hens kept in the aviary system compared to the cage system.

Campylobacter infections in four poultry species in respect of frequency, onset of infection and seasonality.

Over a seven-year period (2001-2007) flocks of four poultry species, 52 broiler flocks, 46 Pekin duck flocks, 22 Muscovy duck flocks, 20 turkey flocks, which were kept on the same farm, were continuously investigated for Campylobacter (C). Altogether 76.1% of the broiler flocks, 59.6% of the Pekin duck flocks, 68.2% of the Muscovy duck flocks and 90.0% turkey flocks were Campylobacter positive. The prevalence during the course of the fattening period increased steadily. There was no specific point of time for the onset of infection. More detailed examination over a one-year period showed the highest isolation rates of C. coli from July to September and a higher isolation rate of the same agent with increasing age, in all species except Muscovy ducks. Moreover, C. coli was isolated more often from the lungs of broilers and Muscovy ducks than from the other two bird species. Flocks of all species housed during the summer months featured a higher prevalence of Campylobacter colonisation than those housed in winter. This was statistically significant for broilers. Another approach for evaluating the seasonality of Campylobacter colonisation was to compare the age of the respective poultry species when the onset occurred in summer and in winter. All poultry species were younger when infection was introduced into a flock in summer. This was statistically significant for broilers and for Pekin ducks.