ABSTRACT
Although it is known that human leukocyte antigen (HLA)-DPB1 disparity has a strong impact on outcomes in unrelated hematopoietic transplantation with induction of acute graft-versus-host disease (GVHD) and a graft-versus-leukemia (GVL) effect, its role in unrelated umbilical cord blood transplantation (UR-CBT) has yet to be fully clarified. Our current study is being conducted to elucidate the impact of HLA-DPB1 mismatch, along with the effect of other HLA loci mismatches at the allele level. HLA six loci alleles were retrospectively typed in 1157 Japanese donors and patients with leukemia or myelodysplastic syndrome who underwent transplantation with a single unit of cord blood. HLA-DPB1 mismatch was associated with a significant reduction in leukemia relapse (hazard ratio 0.61, P<0.001), whereas the other HLA loci allele-level mismatches did not. No significant effect of HLA-DPB1 mismatch was observed in the risk of acute GVHD, engraftment or mortality. This HLA-DPB1 GVL effect without induction of severe acute GVHD or deterioration of survival rate has not been reported in unrelated bone marrow or peripheral blood stem cell transplantations, suggesting apparent advantages of UR-CBT. Accordingly, selection of an HLA-DPB1 mismatch cord blood might be the preferable choice for single-unit UR-CBT.
Subject(s)
Graft vs Host Disease/immunology , Graft vs Leukemia Effect/immunology , HLA-DP beta-Chains/immunology , Leukemia/immunology , Adolescent , Adult , Aged , Aged, 80 and over , Alleles , Bone Marrow Transplantation/methods , Child , Child, Preschool , Cord Blood Stem Cell Transplantation/methods , Female , Hematopoietic Stem Cell Transplantation/methods , Humans , Infant , Infant, Newborn , Male , Middle Aged , Myelodysplastic Syndromes/immunology , Peripheral Blood Stem Cell Transplantation/methods , Transplantation, Homologous/methods , Unrelated Donors , Young AdultABSTRACT
A simple and novel genotyping method was developed to detect alleles at the swine leukocyte antigen (SLA)-DRB1 and -DQB1 class II loci by using polymerase chain reaction (PCR)-fluorescently labeled sequence-specific oligonucleotide probes (SSOPs) and Luminex 100 xMAP detection. The PCR-SSOP-Luminex method exhibited accuracy of 95% for both SLA-DRB1 and -DQB1 in 6 homozygous and 16 heterozygous pig samples as confirmed by sequencing the PCR products of the same samples. In addition, 12 low-resolution SLA class II haplotypes consisting of 7 and 9 DRB1 and DQB1 alleles were identified, respectively, in one population of 283 Landrace pigs. This genotyping method facilitates the rapid and accurate identification of two- or four-digit alleles at the SLA-DRB1 and -DQB1 loci.
Subject(s)
Histocompatibility Antigens Class II/genetics , Polymerase Chain Reaction/methods , Swine/genetics , Animals , Gene Frequency , Genetic Loci , Genotype , Histocompatibility Antigens Class I , Histocompatibility Antigens Class II/immunology , Histocompatibility Testing/methods , Histocompatibility Testing/veterinary , Oligonucleotide Probes/genetics , Polymerase Chain Reaction/instrumentation , Substrate Specificity/genetics , Swine/immunologySubject(s)
Argininosuccinate Synthase/isolation & purification , Ligases/isolation & purification , Liver/enzymology , Animals , Argininosuccinate Synthase/immunology , Argininosuccinate Synthase/metabolism , Humans , Isoelectric Point , Kinetics , Macromolecular Substances , Molecular Weight , Peptide Fragments/analysis , RatsSubject(s)
Acrylic Resins , Dental Cements , Polycarboxylate Cement , Silicate Cement , Zinc Phosphate Cement , SolubilityABSTRACT
This paper reports the clinical trial of amoxicillin. (1) A daily dose of 750 approximately 1,000 mg of amoxicillin was orally administered for 3 approximately 12 days to 20 patients, 7 of which were with acute suppurative tympanitis, 3 with furuncle of the ear, 2 with furuncle of the nose and 8 with acute amygdalitis. As a result, amoxicillin proved remarkably effective or effective in 17 patients (85.0%). (2) Amoxicillin was effective against: Staphylococcus aureus in 10 of 11 patients (90.9%) Staphylococcus epidermidis in 2 of 2 patients (100%) Streptococcus hemolyticus in 3 of 4 patients (75%) Streptococcus viridans in 1 of 1 patient (100%) Diplococcus pneumoniae in 1 of 1 patient (100%) (3) Although one patient developed drug eruption, there were no side effects that necessitated cessation of administration. From the above results, it is concluded that amoxicillin can be used in the treatment of otorhinolaryngologic infections.