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Arq. bras. med. vet. zootec. (Online) ; 74(1): 133-140, Jan.-Feb. 2022. tab, ilus
Article in English | VETINDEX | ID: biblio-1374406

ABSTRACT

κ-Cn and ß-lactoglobulin are important candidate genes associated with milk yield and milk protein content. The present investigation is carried out to determine the polymorphisms status of κ-Cn and ß-lactoglobulin genes in Anatolian Black cattle and Holstein breeds. PCR-RFLP technique was used to determine Kappa-Casein and ß-lactoglobulin polymorphisms in both cattle breeds. The allele frequency of Anatolian Black cattle in terms of κ-Cn and ß-lactoglobulin genes were 0.50 (A) 0.50 (B) and 0.20 (A) 0.80 (B) respectively, whereas in Holstein were 0.29 (A) 0.71 (B) and 0.44 (A) 0.56 (B) respectively. The chi-square test showed that each cattle breed was in Hardy-Weinberg equilibrium (P>0.05).


κ-Cn e ß-lactoglobulina são importantes genes candidatos associados à produção de leite e ao teor de proteína do leite. A presente investigação é realizada para determinar o status de polimorfismos dos genes κ-Cn e ß-lactoglobulina nas raças Anatolian Black cattle e Holstein. A técnica PCR-RFLP foi utilizada para determinar os polimorfismos Kappa-Casein e ß-lactoglobulina em ambas as raças de gado. A freqüência dos alelos do gado negro anatólio em termos de κ-Cn e ß-lactoglobulina foi de 0,50 (A) 0,50 (B) e 0,20 (A) 0,80 (B) respectivamente, enquanto que em Holstein foi de 0,29 (A) 0,71 (B) e 0,44 (A) 0,56 (B) respectivamente. O teste do qui-quadrado mostrou que cada raça bovina estava em equilíbrio de Hardy-Weinberg (P>0,05).


Subject(s)
Animals , Cattle , Caseins/isolation & purification , Caseins/genetics , Lactoglobulins/isolation & purification , Lactoglobulins/genetics , Turkey , Polymerase Chain Reaction , Whey Proteins/isolation & purification , Whey Proteins/genetics
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