The multistressor effect of pH reduction, microplastic and lanthanum on sea urchin Arbacia lixula.

pH reduction (Low pH), microplastic (MP), and lanthanum (La) are substantial stressors due to their increasing trends in marine ecosystems and having adverse effects on marine species. This study investigates the single and combined effects of those stressors (Low pH: 7.45, polyethylene MP: 26 µg L-1, and La: 9 µg L-1) on the physiology and histology of sea urchin Arbacia lixula. Regarding physiological results, while the coelomocytes' quantity was slightly affected by stressors, their viability was significantly affected. The coelomocyte count and viability were suppressed most in Low pH-MP-La treatment. The stressors did not impact the respiration rate. According to the histological examination results, the crypt (villi-like structure) was shorter, and epithelial layers were thinner in single and dual stress treatments like MP, Low pH, Low pH-La, and MP-La. Overall, we suggest that the combination of variable types of those stressors causes negative effects on sea urchin's physiology and histology.

Assessment of Be-7, Pb-210 and Po-210 Activities in Airborne Particulate Matter Over Istanbul, Türkiye.

Airborne particulate matter is one of the air pollutants which can have detrimental health effects in the human body. Radionuclides adsorb onto air particles and make their way to humans primarily through inhalation. Naturally-occurring radionuclides, 210Pb and 210Po, are of notable health concern due to their relatively elevated ingestion and inhalation doses. In the current study, activity concentrations of 7Be, 210Pb and 210Po were determined in air particulate matter (PM). PM2.5 was collected on the European side, while PM10 was collected on the Anatolian side of Istanbul. Be-7, 210Pb and 210Po activities were found to be 5.17 ± 2.35, 0.96 ± 0.42; 0.25 ± 0.14 mBq m- 3 in Anatolian side, respectively. Be-7, 210Pb and 210Po activities were found to be 3.81 ± 2.27, 0.62 ± 0.29, 0.29 ± 0.26, mBq m- 3 in European side, respectively. The ratio of 210Po/210Pb was found to be higher (0.47 ± 0.31 for PM2.5 and 0.34 ± 0.27 for PM10) than the global average of 0.1. This result can be explained by the fact that Po is more volatile than Pb and enhanced in the air by the combustion process. Inhalation dose rates of 210Pb and 210Po due to PM10 exposure were calculated to be 7.70 ± 3.30 and 4.05 ± 2.31 µSv year- 1, respectively. Pb-210 bioaccessibility was assessed by the extraction of the particles in simulated lung fluids. Approximately 24.8% of inhaled 210Pb was estimated to be bioaccessible. This study suggests that 210Po and 210Pb activities are partially enhanced in the air particles in Istanbul and should be regularly monitored.

The combined effects of polyethylene microplastics and benzoanthracene on Manila clam Ruditapes philippinarum.

Microplastic (MP) toxicity has recently been explored in various marine species. Along with the toxicity of plastics polymer itself, additional substances or pollutants that are absorbed onto it may also be harmful. In the present study, we investigated the combined impacts of polyethylene microplastics (PE MPs) and an organic pollutant (Benzo(a)anthracene, BaA) on Manila clam Ruditapes philippinarum during a one-week exposure. Two PE MPs concentrations (26 µg L-1 and 260 µg L-1) and one BaA concentration (3 µg L-1) were tested. The clams were exposed to BaA and PE MPs either alone or in combination. BaA and PE MPs were incubated before the combined exposure. The biological effects of PE MPs and BaA on the clams were evaluated by considering several assays such as feeding rate, anti-oxidant enzyme activities, and the expression levels of stress-related genes. The feeding rate significantly decreased in individual PE MPs and individual BaA groups while it remained unchanged in combined groups. Superoxide dismutase (SOD) was the most affected among the biochemical parameters. Malondialdehyde (MDA), and glutathione peroxidase (GPx) activities were slightly affected, whereas no changes were observed in glutathione s-transferase (GST) activities. CYP1A1, CYP3A4, and HSP70 gene expressions displayed slightly significant changes. Considering all stressor groups, high PE MPs exposure (260 µg L-1 PE MPs) more effectively altered the biological parameters in the clams compared to individual low PE MPs and BaA exposure, and their combination. The results also indicated the negligible vector role of PE MPs to transport BaA into the clam tissues.

Physiological and gene expression responses of the mussel Mytilus galloprovincialis to low pH and low dissolved oxygen.

The prevalence and frequency of hypoxia events have increased worldwide over the past decade as a consequence of global climate change and coastal biological oxygen depletions. On the other hand, anthropogenic emissions of CO2 and consequent accumulation in the sea surface result in a perturbation of the seawater carbonate system, including a decrease in pH, known as ocean acidification. While the effect of decreases in pH and dissolved oxygen (DO) concentration is better understood, their combined effects are still poorly resolved. Here, we exposed adult mussels (Mytilus galloprovincialis) to two pHs (8.27 and 7.63) and DO concentrations (7.65 and 2.75 mg L-1) over 17 days in a full-factorial design. These levels correspond to extremes of the present natural variability and are relevant in the context of ocean acidification and hypoxia. No mortality was observed during the experiment. However, sublethal effects were observed for clearance and oxygen consumption rates, as well as total haemocytes count and haemocytes viability and gene expression in mussels exposed to the combination of low pH and low DO. Respiration and excretion rates were not significantly impacted by low pH and DO, alone or in combination. Overall, low pH alone led to a decrease in all tested physiological parameters while low DO alone led to a decline in clearance rate, haemocyte parameters and an increase in carbohydrate content. Both parameters led to up- or down-regulation of most of the selected genes. Not surprisingly, the combined effect of low pH and low DO could not be predicted by a simple arithmetic additive response at the effect level, highlighting more complex and non-linear effects.

Chronology of trace elements and radionuclides using sediment cores in Golden Horn Estuary, Sea of Marmara.

Trace elements and radionuclides are substantial pollutants in marine environment since they are non-biodegradable and can be harmful even in minute concentrations. The Golden Horn estuary, where is an inlet of Bosphorus and two creeks, has been seriously polluted by untreated municipal and industrial dischargers for several decades. Since 1998, a large restoration and rehabilitation efforts have been undertaken in the estuary to mitigate the pollution. In the present study, four sediment cores were taken from the Golden Horn estuary to assess the historical accumulation of trace elements and radionuclides. Radiometric dating was implemented by 210Pb and 137Cs radionuclides and CRS model. Sedimentation rates were calculated in the range of 0.92-0.97 cm yr-1 in the estuary. The distribution of radionuclides (40K, 226Ra, and 228Ra) indicated some slight variations which ascribes to the geological characteristics of sediment along the cores. The concentrations of the anthropogenic elements were relatively higher in the intensive industrialization period. Their concentrations reduced in the latest 15-20 years thanks to the large-scale rehabilitation project in the estuary. The pollution indices, namely EF, Igeo, CF, and PLI showed that the concentrations of Cd, Cr, Cu, Pb, Sb, and Sn were above the world averages. Our results provide an insight on the long-term accumulation trends of trace element in the Golden Horn, which revealed that the estuary remains moderately polluted. We suggest that preventive countermeasures are much more important than post pollution remediation in the case of metallic pollution in the estuaries.

Microplastic inventory in sediment profile: A case study of Golden Horn Estuary, Sea of Marmara.

Assessment of microplastics (MPs) in sediment cores is necessary to unveil global plastic pollution since most of the plastic litter might have been stored in sediment columns. In the current study, MPs inventory was determined in a 105 cm sediment core, collected in the Golden Horn Estuary, Sea of Marmara. Radiodating of sediment profile by using naturally occurring 210Pb and fission product 137Cs allowed us to couple the retrospective of global MP production to sediment MPs inventory. More than 90% of total MPs inventory was found in the deep layer of the sediment column (below 15 cm). Small MPs (20-200 µm) were more abundant than large ones (200-4000 µm). Elevated concentrations of MPs were attributed to industrial and municipal effluent of Istanbul metropolitan. On a local scale, this study suggests that the Golden Horn Estuary was polluted with MPs before the 1950s, and the abundance of MPs reached a maximum in the 1980s. We also propose on a global scale that "the missing" plastics might have been buried in deep sediment and radiodating of sediment is useful to reveal their historical input records.

DETERMINATION OF RA-226, TH-232, K-40 AND CS-137 ACTIVITIES IN SOILS AND BEACH SANDS AND RELATED EXTERNAL GAMMA DOSES IN ARIKLI MINERALIZATION AREA (AYVACIK/TURKEY).

Uranium mineralization areas may jeopardize public health and surrounding natural life. In the current study for radioactivity and geochemical analyses, 43 soil, 5 rock and 10 beach sand samples were collected in and around Arikli (Ayvacik, Turkey) uranium mineralization area. Radioactivity levels in the soils (up to Ra-226: 629, Th-232: 240, K-40: 3669 Bq/kg) were found higher than the that of beach sands. Besides, radioactivity of the beach sand samples is found at normal levels (avg: Ra-226: 31, Th-232: 31, K-40: 542 Bq/kg) when compared to worldwide average soil. Dose values indicate that the region have ~three times higher average gamma radioactivity than the corresponding world average although measured gamma doses ranged up to 687 nGy/h. The high measured values are related to uranium mineralization, alteration zones, faults and Arikli tuff.

Genotoxic and cytotoxic effects of polyethylene microplastics on human peripheral blood lymphocytes.

Currently, we need emerging initial data regarding how plastic exposures affect cellular and molecular components and how such interactions will be crucial for human health. We aimed to determine the genotoxic and cytotoxic effects of microplastic (MPs,10-45 µm, polyethylene) on human peripheral lymphocytes by using the cytokinesis-block micronucleus cytome (CBMN) assay, which is a comprehensive method to reveal a range of mechanisms, not only diseases but also response to environmental exposures. We measured micronucleation (MN), nucleoplasmic bridge formation (NPB), and nuclear bud formation (NBUD) in human peripheral blood lymphocytes. We also measured the cytokinesis-block proliferation index (CBPI) to calculate cytostasis, which indicates cytotoxicity in lymphocytes treated with five different MPs concentrations for 48 h. Even lower concentrations of MPs increased the level of genomic instability. We found that the in vitro MP exposure significantly increased MN, NPB, and NBUD frequencies. Since we investigated the effect of larger particles relative to the lymphocytes, mechanic interaction of MPs with cells, the release of monomer and additives from MPs could be suggested as possible mechanisms accounting for increasing genomic instabilities. We did not observe a decrease in the cell proliferation index, indicating a lack of MPs' cytotoxic potential. To the best of our knowledge, our study is the first to identify MPs' genotoxic potential in human peripheral blood lymphocytes. We suggested further studies to investigate the genotoxic and cytotoxic potential of smaller plastics and the chronic effect of MP on the human population.

Impacts of elevated pCO2 on Mediterranean mussel (Mytilus galloprovincialis): Metal bioaccumulation, physiological and cellular parameters.

Ocean acidification alters physiology, acid-base balance and metabolic activity in marine animals. Near future elevated pCO2 conditions could be expected to influence the bioaccumulation of metals, feeding rate and immune parameters in marine mussels. To better understand such impairments, a series of laboratory-controlled experiment was conducted by using a model marine mussel, Mytilus galloprovincialis. The mussels were exposed to three pH conditions according to the projected CO2 emissions in the near future (one ambient: 8.10 and two reduced: 7.80 and 7.50). At first, the bioconcentration of Ag and Cd was studied in both juvenile (2.5 cm) and adult (5.1 cm) mussels by using a highly sensitive radiotracer method (110mAg and 109Cd). The uptake and depuration kinetics were followed 21 and 30 days, respectively. The biokinetic experiments demonstrated that the effect of ocean acidification on bioconcentration was metal-specific and size-specific. The uptake, depuration and tissue distribution of 110mAg were not affected by elevated pCO2 in both juvenile and adult mussels, whereas 109Cd uptake significantly increased with decreasing pH in juveniles but not in adults. Regardless of pH, 110mAg accumulated more efficiently in juvenile mussels than adult mussels. After executing the biokinetic experiment, the perturbation was sustained by using the same mussels and the same experimental set-up, which enabled us to determine filtration rate, haemocyte viability, lysosomal membrane stability, circulating cell-free nucleic acids (ccf-NAs) and protein (ccf-protein) levels. The filtration rate and haemocyte viability gradually decreased by increasing pCO2 level, whereas the lysosomal membrane stability, ccf-NAs, and ccf-protein levels remained unchanged in the mussels exposed to elevated pCO2 for eighty-two days. This study suggests that acidified seawater partially shift metal bioaccumulation, physiological and cellular parameters in the mussel Mytilus galloprovincialis.

Influence of pH on Pb accumulation in the blue mussel, Mytilus edulis.

Changes in seawater pH can alter the chemical speciation of waterborne chemical elements, affecting their bioavailability and, consequently, their bioaccumulation in marine organisms. Here, controlled environmental conditions and a 210Pb radiotracer were used to assess the effect of five distinct pH conditions (pHT ranging from 7.16 to 7.94) on the short-term (9 days) accumulation of Pb in the blue mussel, Mytilus edulis. After 9 days of exposure, higher levels of Pb were observed in the soft tissues of mussels maintained in the lower pH conditions, while Pb levels accumulated by mussel shells showed no difference across pH conditions. These results suggest that pH decreases such as those predicted by ocean acidification scenarios could enhance Pb contamination in marine organisms, with potential subsequent contamination and effect risks for human consumers.

Effects of variable deoxygenation on trace element bioaccumulation and resulting metabolome profiles in the blue mussel (Mytilus edulis).

The dissolved oxygen concentration of the world's oceans has systematically declined by 2% over the past 50 years, and there has been a notable commensurate expansion of the global oxygen minimum zones (OMZs). Such wide-scale ocean deoxygenation affects the distribution of biological communities, impacts the physiology of organisms that may affect their capacity to absorb and process contaminants. Therefore, the bioaccumulation efficiencies of three contrasting radionuclides, 110mAg, 134Cs and 65Zn were investigated using controlled aquaria in the blue mussel Mytilus edulis under three contrasting dissolved oxygen regimes: normoxic; 7.14 mg L-1, reduced oxygen; 3.57 mg L-1 and hypoxic 1.78 mg L-1 conditions. Results indicated that hypoxic conditions diminished 110mAg uptake in the mussel, whereas depuration rates were not affected. Similarly, hypoxia appeared to cause a decrease in the 65Zn bioaccumulation rate, as evidenced by both weakened uptake and rapid elimination rates. Effects of hypoxia on the metabolome of mussels were also explored by untargeted Nuclear Magnetic Resonance (NMR) spectroscopic methods. The metabolic response was characterised by significantly greater abundance of several amino acids, amino sulfonic acids, dicarboxylic acids, carbohydrates and other metabolites in the lowest oxygen treatment, as compared to the higher oxygen treatments. Clearance rates significantly dropped in hypoxic conditions compared to normoxia. Results suggest that hypoxic conditions, and even partly moderate hypoxia, alter ventilation, an-aerobic, oxidative and osmoregulation metabolism of this mussel, which may further influence the trace element bioaccumulation capacity.

Effects of microplastics and mercury on manila clam Ruditapes philippinarum: Feeding rate, immunomodulation, histopathology and oxidative stress.

Plastic pollution, which is one of the most important environmental problems at the present time, has been understood recently, and the effects of this pollution on ecosystem and biota are becoming a growing problem, especially in the aquatic ecosystems. Direct or indirect exposure to those particles leads to adverse effects on marine organisms. In the marine environment, plastic materials interact with other pollutants such as metals, thereby affecting the uptake levels of those pollutants in marine organisms. In the present study, the Manila clam Ruditapes philippinarum was exposed to polyethylene microbeads and mercury chloride in single, combined and incubated form at environmentally relative concentrations for one week in controlled laboratory conditions. The uptake and tissue distribution of both stressors as well as the vector role of microplastics on mercury uptake in the organisms were investigated. Filtration rates, biomarkers for immunomodulation and oxidative stress, and histological alterations were also evaluated. Microplastics were ingested by the clams, and translocated to the various tissues. However, contaminated microplastics displayed a negligible vector role in terms of mercury bioaccumulation in the clams. The single and interactive exposure of the stressors reduced the filtration rate in the clams. Both pollutants affected the immune system of the organisms. Histological alterations were determined in the gill and digestive gland tissues of the clams among the treatment groups, although oxidative stress biomarkers remained unchanged. This study suggests that the vector role of polyethylene microplastics in mercury uptake is negligible and reveals that the single and interactive one-week exposure of two pollutants induce toxicity in the manila clams.

Mercury and Po-210 in mollusc species in the island of Gökçeada in the north-eastern Aegean Sea: Bioaccumulation and risk assessment for human consumers.

Among the radioactive pollutants 210Po is the most substantial one in terms of seafood safety due to its efficient accumulation in marine animals and high irradiation of its alpha emission. Mercury is a highly toxic metal for both marine organisms and human beings. Biomagnification of MeHg (methylmercury) through marine food chains has made Hg concern of ecotoxicology and seafood safety. In the current study, the bioaccumulation of 210Po and THg (total mercury) were determined in 20 mollusc species, including 8 bivalves, 7 gastropods and 5 cephalopods collected from the island of Gökçeada in the north-eastern Aegean Sea. The highest accumulation of 210Po and Hg was seen in bivalves and cephalopods, respectively. Elevated Hg concentrations in all body parts (arms, mantle and viscera) were observed in octopus' species. The results of this study suggests that filter feeder bivalves and gastropods have a capacity to concentrate 210Po in their bodies, whereas predator gastropods and cephalopods have a capacity to concentrate Hg in their bodies. 7.0 kg (3.2-14.2) bivalve flesh intake is adequate due to 210Po ingestion in the studied region to reach 1 mSv which is the annual committed effective dose. Octopus consumption of 705 g in a week alone is needed to reach Provisional Tolerable Weekly Intake (PTWI) of mercury, 5 µg kg-1 body weight. Due to very low non-fish seafood consumption in Turkey there is no risk of Hg intake and alpha radiation of 210Po above the limit values through mollusc consumption.

Multi-pollutant Monitoring in a Rehabilitated Estuary: Elements and Radionuclides.

The Golden Horn Estuary, Istanbul, Turkey, had been exposed to untreated industrial discharges and municipal wastewater for over fifty years; however, during the last fifteen years pollution has been reduced due to an extensive rehabilitation campaign. Since sediment is the main accumulation matrix of marine ecosystems, surface sediment samples were collected from the estuary and analyzed for concentrations of some elements and radioactivity. The elements of predominant anthropogenic origin such as As, Cu, Pb, Sb, Sn and Zn and 137Cs were observed in the mid-estuary. Also, elevated geoaccumulation indices for Ag, Cd, Cu and Sb showed that pollution was still occurring in the estuary despite the rehabilitation. There is a huge residential area near the estuary; therefore, people living in this area are affected due to the pollution of the estuary. Thus, the obtained data of the elements and radionuclides are very important for the ecosystem of the Golden Horn.

Effects of ocean acidification on 109Cd, 57Co, and 134Cs bioconcentration by the European oyster (Ostrea edulis): Biokinetics and tissue-to-subcellular partitioning.

The uptake and depuration kinetics of dissolved 109Cd, 57Co and 134Cs were determined experimentally in the European flat oyster Ostrea edulis (Linnaeus, 1758) under different pH conditions (i.e., 8.1, 7.8 and 7.5) for 59 days. Uptake and depuration rates were variable within these elements; no effects were observed under different pH conditions for the uptake biokinetics of 109Cd and 57Co and depuration of 109Cd and 134Cs in oyster. The uptake and depuration rate constants of 134Cs differed during the exposure phase between treatments, while the steady state concentration factors (CFss) were similar. The resulting Cs activity that was purged during short- and long-term depuration phases differed, while the remaining activities after thirty-nine days depuration phase (RA39d) were similar. Co-57 depuration was affected by pCO2 conditions: RA39d were found to be significantly higher in oysters reared in normocapnia (pCO2 = 350 µatm) compared to high pCO2 conditions. Co-57 tissue distribution did not differ among the variable pCO2 conditions, while 109Cd and 134Cs accumulated in soft tissue of oysters were found to be higher under the highest pCO2. Additionally, Cd, Co and Cs were stored differently in various compartments of the oyster cells, i.e. cellular debris, metal-rich granules (MRG) and metallothionein-like proteins (MTLP), respectively. The subcellular sequestration of the elements at the end of the depuration phase did not differ among pH treatments. These results suggest that bioconcentration and tissue/subcellular distribution are element-specific in the oyster, and the effects of higher pCO2 driven acidification and/or coastal acidification variably influence these processes.

Geochronology and sources of heavy metal pollution in sediments of Istanbul Strait (Bosporus) outlet area, SW Black Sea, Turkey.

Geochemical and sedimentological analyses and radionuclide (210Pb and 137Cs) dating of three cores from the Bosporus outlet area of the Black Sea, north of Istanbul, were conducted to assess the sources and history of heavy metal pollution. The sedimentary succession in the shelf core KD12-01 consists mainly of clay (49-80%) and silt (15-41%). Radionuclide dating of the core indicates that it consists of old sediments that are uncontaminated with heavy metals. In contrast, cores KD12-04 and KD12-07 recovered from -350 m and -304 mm in the upper slope area represent sediments consisting of silt and clay that were deposited since at least the last 120 years and 60 years, respectively. The latter core contains two mass-flow units represented by relatively old sedimentary material according to the low 210Pb activity and relatively low heavy metal contents. The upper 40 and 48 cm of cores KD 12-04 and KD 12-07 represent sediments deposited since 1970s and 1980s that are significantly polluted with Cu, Ni, Zn, Mo, Pb and Cr, Cu, Co, Ni, Mo, Pb, Zn, respectively. However, high Pb and Cr concentrations with high TOC contents date back to early part of the 20th century in core KD 12-04. The geochemical data, together with the high 137Cs concentrations of the contaminated sediments, strongly suggest that the pollution is mainly delivered to the western and north western Black Sea by the large European rivers, from there transported to the study area by the rim current, and deposited in the sediments under anoxic conditions.

Acidified seawater increases accumulation of cobalt but not cesium in manila clam Ruditapes philippinarum.

The pH of seawater around the world is expected to continue its decline in the near future in response to ocean acidification that is driven by heightened atmospheric CO2 emissions. Concomitantly, economically-important molluscs that live in coastal waters including estuaries and embayments, may be exposed to a wide assortment of contaminants, including trace metals and radionuclides. Seawater acidification may alter both the chemical speciation of select elements as well as the physiology of organisms, and may thus pose at risk to many shellfish species, including the manila clam Ruditapes philippinarum. The bioconcentration efficiency of two common radionuclides associated with the nuclear fuel cycle, 134Cs and 57Co, were investigated by exposing live clams to dissolved 134Cs and 57Co at control (pH = 8.1) and two lowered pH (pH = 7.8 and 7.5) levels using controlled aquaria. The uptake and depuration kinetics of the two radionuclides in the whole-body clam were followed for 21 and 35 days, respectively. At steady-state equilibrium, the concentration factor (CFss) for 57Co increased as the pH decreased (i.e. 130 ± 5, 194 ± 6, and 258 ± 10 at pH levels 8.1, 7.8 and 7.5, respectively), whereas the 134Cs uptake was not influenced by a change in pH conditions. During depuration, the lowest depuration rate constant of 57Co by the manila clam was observed at the intermediate pH of 7.8. An increase in the accumulation of 57Co at the intermediate pH value was thought to be caused mainly by the aragonitic shell of the clam, as well as the low salinity and alkalinity of seawater used in the experiment. Considering that accumulation consists of uptake and depuration, among the three pH conditions moderately acidified seawater enhanced most the accumulation of 57Co. Accumulation of 134Cs was not strongly influenced by a reduced pH condition, as represented by an analogous uptake constant rate and CFss in each treatment. Such results suggest that future seawater pH values that are projected to be lower in the next decades, may pose a risk for calcium-bearing organisms such as shellfish.

Trophic transfer of 134Cs in the Manila clam Ruditapes philippinarum.

Bioaccumulation of radiocaesium in many marine organisms occurs through complex trophic transfer mechanisms. The present study addresses the trophic transfer of 134Cs in the widely distributed marine bivalve, the Manila clam Ruditapes philippinarum, by experimentally determining the assimilation efficiency (AE) and the specific role of food quality or diet on the AE in this marine invertebrate. Pulse-chase feeding experiments were carried out on this clam using the phytoplankton species Tetraselmis chuii, Phaeodactylum tricornutum and Isochrysis galbana. Depuration kinetics of 134Cs over 21 days were analysed using a two-component exponential model. Observed assimilation efficiencies were consistently less than 10% but slightly varied among individuals fed on the three different phytoplankton species diets (T. chuii: AE = 8.4 ± 0.6%; P. tricornutum: AE = 9.8 ± 0.5%; I. galbana: AE = 5.3 ± 0.6%), although no statistical differences were observed. Comparing results from these experiments with existing data from the literature on the same species exposed to caesium through seawater, it appears that trophic transfer processes are the main accumulation pathway, contributing up to 96% of the global 134Cs bioaccumulation in this bivalve species.

Assessment of metal concentrations in indigenous and caged mussels (Mytilus galloprovincialis) on entire Turkish coastline.

This survey was conducted to determine metal concentrations in the biomonitor organism Mytilus galloprovincialis collected from all coasts of Turkey. Naturally-occurring mussels were gathered along the coast of the Black Sea, Sea of Marmara, Bosphorus and Aegean Sea, while a 6-month transplantation session was implemented on the Levantine Sea coast where M. galloprovincialis does not adapt naturally. Mussels collected from the Izmir area were employed for transplantation to five locations along the Levantine Sea coast of Turkey. Concentrations of Ag, Al, As, Cd, Co, Cr, Cu, Fe, K, Mn, Ni, Pb, Sn, V, and Zn were measured using ICP-MS (Inductively Coupled Plasma-Mass Spectrometry). Results showed that concentrations of some essential elements (Co, Fe, K, Mn, Zn) generally fell within the range of acceptable global values. However, at some sampling sites the metal concentrations were found at higher levels compared to the world average and recommended limits. For instance, very high Ag and Cu concentrations were observed in mussel caged at Yumurtalik. Elevated As, Ni, Pb, and V concentrations were observed at the Tirebolu, Eregli, Eskihisar, and Tekirdag locations, respectively. Taking into account all measured metals, it can be inferred that Yumurtalik is the most metal-contaminated locality. The results highlighted metal-specific pollution at coastal areas in each sea cover Turkey. In spite of the high metal levels estimated daily intakes (EDI) of the measured elements were well below through very low mussel consumption by Turkish public.

Radioactivity levels in mussels and sediments of the Golden Horn by the Bosphorus Strait, Marmara Sea.

The Golden Horn is an estuary located in the center of Istanbul receiving freshwater discharges from two creeks and connecting to the Bosphorus Strait. Activity concentrations of natural and artificial radionuclides were determined in mussels (Mytilus galloprovincialis) and sediments from the Golden Horn sampled in February 2012. Mean activity concentrations of (137)Cs, (40)K, (226)Ra, (228)Ra, (210)Po and (210)Pb in the mussels were determined at 1.03±0.23, 389±41.6, 2.61±1.23, not detected (ND), 91.96±37.88 and 11.48±4.85 Bq kg(-1), respectively. In sediments, it was observed that (137)Cs, (40)K, (226)Ra, (228)Ra, (210)Po and (210)Pb activity concentrations in<63 µm particle fraction of sediment were generally higher than those determined in mussels. Po-210 and (210)Po/(210)Pb ratios in mussels from the Golden Horn were much lower than in mussels from other coastal regions and this was related to low plankton productivity and eutrophication of the Golden Horn.