ABSTRACT
Investigated 62 sample of thyroid gland obtained after surgical intervention, including: HT (n=27), RT (n=9), Graves' disease (n=17) and papillary thyroid carcinoma (n = 10). The slides were studied using classical histological and immunohistochemical methods: H&E, TTF1, TSH, S100-protein, CD56 and p63. Dispite of the histological and immunohistochemical heterogeneity of Thyroiditis HT and RT, the progressive involution of the glandular tissue with the replacement by the sever fibrosis, in some cases by the scar tissue is observed as damage sign. In Hashimoto thyroiditis, the foci of follicular epithelium dysplasia were determined, with p63 positive and CD56 negative reactions. Graves' disease is characterized by high TSH expression as well as lymphoproliferation with the formation of large fused nodules with germinative centers. With Riedel's thyroiditis, there is a moderate expression of TTF-1 in the stroma and capillary endotheliocytes, as well as diffuse-focal moderate expression of S100 protein in cells of neuroectodermal population. The reaction to malignant transformation markers - CD56 and p63 - in the tissue of Thyroid gland with Thyroiditis, Riedel was definitely negative.
Subject(s)
Hashimoto Disease/metabolism , Thyroid Cancer, Papillary/metabolism , Thyroid Gland/metabolism , Thyroid Neoplasms/metabolism , Thyroiditis/metabolism , Autoimmune Diseases , Hashimoto Disease/pathology , Humans , Receptors, Thyrotropin/metabolism , S100 Proteins/metabolism , Thyroid Cancer, Papillary/pathology , Thyroid Neoplasms/pathology , Thyroid Nuclear Factor 1/metabolism , Thyroiditis/pathologyABSTRACT
The aim of our study was to investigate the characteristics of proliferation activity in squamous metaplasia and reserve cell hyperplasia of uterine cervix. Four groups of patients were selected: 1st group--34 reproductive patients with reserve cell hyperplasia, 2nd group--21 reproductive patients with squamous metaplasia, 3rd group--12 postmenopausal patients with reserve cell hyperplasia, 4th group--29 postmenopausal patients with squamous metaplasia. These groups included the patients with and without infections. Cervical biopsies were studied by immunohistochemistry using anti-Ki-67 monoclonal antibody. Ki-67(+) reserve cells were counted in reserve cell hyperplasia. Ki-67(+) squamous cells were counted in squamous metaplasia. The results showed that in both squamous metaplasia and reserve cell hyperplasia the number of Ki-67(+) cells, i.e. the intensity of proliferation is depended on the co-existence of infection, as well as on the age of the patient. These data are important in the complex treatment of cervical pseudoerosions.
Subject(s)
Carcinoma, Small Cell/pathology , Carcinoma, Squamous Cell/pathology , Cell Proliferation , Postmenopause , Uterine Cervical Neoplasms/pathology , Adult , Biopsy , Carcinoma, Small Cell/immunology , Carcinoma, Squamous Cell/immunology , Female , Humans , Hyperplasia/immunology , Hyperplasia/pathology , Ki-67 Antigen/immunology , Middle Aged , Uterine Cervical Neoplasms/immunologyABSTRACT
The aim of our study was to identify the morphological characteristics of cervical squamous metaplasia in reproductive women. We have performed the biopsy in patients who had the abnormal transformation zone by colposcopy. Cervical biopsies were studied histologically by HandE. The results showed that cervical squamous metaplasia has different clinical importance in reproductive patients. In reproductive women it is associated with cervical intraepithelial neoplasia. In reproductive women assessment of pseudoerosions has important meaning.
Subject(s)
Carcinoma, Squamous Cell/pathology , Uterine Cervical Neoplasms/pathology , Adult , Algorithms , Biopsy , Carcinoma, Squamous Cell/surgery , Colposcopy , Female , Humans , Neoplasm Staging , Uterine Cervical Neoplasms/surgeryABSTRACT
A thermostable protein complex (TSPC) obtained from the myocardium ventricules of adult rats inhibits mitotic and transcriptional activity of cardiomyocytes. At the same time this complex is more active on early stages of the postnatal ontogenesis in rats, aged 1 and 5 days. Following its action on RNA synthesis, the TSPC reveals tissue specificity only in cells with terminal differentiation, and is determined by its nuclear membranes. We continue studies for identifying the molecular weight and chemical nature of the TSPC, and the role of its different fractions in regulation of proliferation processes. Besides, it is planned to produce antibodies against TSPC fractions with the purpose to block its inhibitory effect on myocyte regeneration in the damaged myocardium.