Subject(s)
Antineoplastic Agents/chemistry , Bridged-Ring Compounds/chemistry , Paclitaxel/analogs & derivatives , Paclitaxel/chemistry , Taxoids , Alkaloids/chemical synthesis , Alkaloids/chemistry , Alkaloids/toxicity , Animals , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/toxicity , Bridged-Ring Compounds/chemical synthesis , Crystallography, X-Ray , Female , Humans , Melanoma, Experimental/drug therapy , Mice , Mice, Inbred Strains , Molecular Conformation , Molecular Structure , Ovarian Neoplasms , Paclitaxel/chemical synthesis , Paclitaxel/toxicity , Tumor Cells, CulturedABSTRACT
The solid-phase synthesis of the N alpha-Fmoc analog of protein kinase C substrate (PKCS, Lys-Arg-Ala-Lys-Ala-Lys-Thr-Thr-Lys-Lys-Arg) was characterized by low recovery from the resin and the concomitant appearance of four impurities. FAB-MS revealed molecular weights for two of these impurities that corresponded to the desired peptide plus Tos or Bzl. The other two were justified by invoking a CO2 elimination of the Clz protecting group to yield: 1) peptide plus 2-chlorobenzyl (ClBzl) and 2) peptide plus ClBzl and Tos. A CF-FAB analysis of carboxypeptidase digestions allowed observation of peptide cleavage down to an ion corresponding to lysine, Fmoc, and the corresponding protecting group(s). These data revealed that the impurities were not the result of incomplete deprotection but the result of migration of the protecting groups to the N-terminal end of the peptide. NMR experiments were subsequently performed and revealed the exact site of substitution: the meta positions of the N-terminal Fmoc. These impurities are presumed to arise by electrophilic aromatic substitution of the fluorene group during HF treatment. The desired Fmoc analog served as a convenient, albeit low-yielding, intermediate for purification of the highly charged PKCS by preparative self-displacement HPLC.
Subject(s)
Amino Acids/chemistry , Carboxypeptidases/chemistry , Fluorenes/chemistry , Hydrofluoric Acid/chemistry , Peptides/chemical synthesis , Amino Acid Sequence , Carbon Radioisotopes , Magnetic Resonance Spectroscopy/methods , Molecular Sequence Data , Oligopeptides/chemical synthesis , Oligopeptides/isolation & purification , Peptides/isolation & purification , Spectrometry, Mass, Fast Atom BombardmentABSTRACT
The use of N alpha-tert.-butyloxycarbonyl-N pi-benzyloxymethylhistidine in peptide synthesis resulted in significant levels of several different side products attributable to the generation of formaldehyde during the hydrogen fluoride cleavage reaction. Methylated impurities in a decapeptide were isolated and identified. These methylated impurities were attributed to the use of the benzyloxymethyl protecting group for the histidines, since the impurities did not form when the dinitrophenyl protecting group was used. Also, peptides containing benzyloxymethyl-protected histidines in addition to N-terminal cysteines quantitatively yielded their respective N-terminal thiazolidine derivatives upon isolation from standard hydrogen fluoride cleavage mixtures. Thiazolidine ring formation was circumvented by including in the cleavage reaction a formaldehyde scavenger such as cysteine hydrochloride or resorcinol.