ABSTRACT
Fundamento: la finalidad de la estrategia curricular Dominio del Idioma Inglés, propuesta por la subcomisión de estrategias curriculares de la Universidad de Ciencias Médicas de la Habana desde el 2009, es la competitividad idiomática de los profesionales de la salud. Objetivo: diseñar acciones metodológicas para la implementación de la estrategia curricular Dominio del Idioma Inglés en la carrera de Medicina de la Filial Universitaria Municipal de Santo Domingo. Métodos: se realizó una investigación de desarrollo con enfoque mixto entre enero de 2011 y diciembre de 2013; se utilizaron métodos teóricos: análisis-síntesis e inducción-deducción y empíricos: análisis documental de la Resolución 210/2007, planes de trabajo metodológico y actas en los diferentes niveles organizativos, modelo del profesional y planes de clase; y la encuesta en forma de entrevista a directivos, y de cuestionario a docentes para identificar las principales insuficiencias en la preparación idiomática y metodológica. Resultados: se constató que las principales insuficiencias están relacionadas con la dirección y ejecución del trabajo metodológico interdisciplinar y con el pobre desarrollo de habilidades para la gestión de la información médica en idioma inglés, por lo que se diseñaron acciones metodológicas para lograr una mayor objetividad en la implementación interdisciplinar de esta estrategia que tribute al actual modelo del profesional de la salud. Conclusiones: las acciones fueron valoradas como pertinentes por criterios de especialistas, porque contribuyeron a transformar progresivamente la realización del trabajo metodológico para desarrollar una competencia idiomática interdisciplinar y profesional como garantía de un desempeño competente en contextos de habla inglesa.
Background: the purpose of the curricular strategy of the English Language command, proposed by the subcommittee of curricular strategies of Havana University of Medical Sciences since 2009 is the idiomatic competence of health professionals. Objective: to design methodological actions for the implementation of the curricular strategy of the English Language command in the Medicine career of Santo Domingo's Municipality Health University Site. Methods: it was carried out a development investigation with a combined approach from January 2011 to December 2013; theoretical methods were used: analysis-synthesis and induction-deduction and empiric ones: documental analysis of the 210/2007 Regulation from the Ministry of Higher Education , methodological plans and meeting records at different organizational levels, model of the professional and lesson plans; and the survey in interview form was applied to administrative leaders and a questionnaire was applied to the professors to identify the main inadequacies in the idiomatic and methodological preparation. Results: it was verified that the main inadequacies are related with the management and execution of the interdisciplinary methodological work and with the poor development of abilities for the medical information management in the English language, that's why methodological actions were designed to achieve a bigger objectivity in the interdisciplinary implementation of this strategy that contributes with the current model of health professionals. Conclusions: the actions were valued as pertinent by the specialists' criteria, because they contributed to transform the work with the methodological activity progressively to develop an interdisciplinary and professional idiomatic competence as guarantee of a competent performance in English-speaking contexts.
Subject(s)
English AbstractABSTRACT
BACKGROUND: It is well documented that Giardia duodenalis undergoes surface antigenic variation both in vivo and in vitro. Proteins involved have been characterized and referred to as VSP (variable surface protein). METHODS: Two cloned cDNA inserts of 0.45 and 1.95 kb were obtained from G. duodenalis expression library and sequenced. Comparison sequence analyses were made against Genbank. PCR analysis was performed on G. duodenalis isolates to identify isolates bearing genes encoding such a peptide. Specific antiserum was prepared against 450-bp encoded peptide and tested by Western blot, immunofluorescence, and inhibition of adhesion of G. duodenalis to target cells. RESULTS: We cloned and characterized a G. duodenalis 450-bp DNA fragment; its DNA sequence analysis revealed that this fragment displayed 99% identity with vsp9B10A gene. Predicted amino acid sequence for this fragment also had significant (99%) identity to VSP9B10A. A second 1.95-kb insert, which encompassed the 450-bp cDNA fragment, was also isolated; its DNA and amino acid sequence displayed 99.5% identity with vsp9B10A gene and 99.2% with the corresponding inferred protein, respectively. This inferred protein contained 24 Cys-X-X-Cys motifs and long ORF of 642 aminoacids. PCR analysis showed that DNA sequence encoding a fragment of this gene was present in P1, CIEA:0487:2-C-8 clone and in INP:180800-B2 G. duodenalis human isolates, while it was absent in sheep isolate of G. duodenalis INP:150593-J10. CONCLUSIONS: Immunofluorescence analysis using antibodies raised against the peptide encoded by 450-bp fragment showed that expression of this epitope varies on trophozoite surface of the C-8 Mexican clone and is involved in parasite adhesion to target epithelial cells.