ABSTRACT
In vitro electrophysiological data suggest that interleukin-1 may promote non-rapid eye movement sleep by inhibiting spontaneous firing of wake-active serotonergic neurons in the dorsal raphe nucleus (DRN). Interleukin-1 enhances GABA inhibitory effects. DRN neurons are under an inhibitory GABAergic control. This study aimed to test the hypothesis that interleukin-1 inhibits DRN serotonergic neurons by potentiating GABAergic inhibitory effects. In vitro intracellular recordings were performed to assess the responses of physiologically and pharmacologically identified DRN serotonergic neurons to rat recombinant interleukin-1beta. Coronal slices containing DRN were obtained from male Sprague-Dawley rats. The impact of interleukin-1 on firing rate and on evoked post-synaptic potentials was determined. Evoked post-synaptic potentials were induced by stimulation with a bipolar electrode placed on the surface of the slice ventrolateral to DRN. Addition of interleukin-1 (25 ng/mL) to the bath perfusate significantly decreased firing rates of DRN serotonergic neurons from 1.3 +/- 0.2 Hz (before administration) to 0.7 +/- 0.2 Hz. Electrical stimulation induced depolarizing evoked post-synaptic potentials in DRN serotonergic neurons. The application of glutamatergic and GABAergic antagonists unmasked two different post-synaptic potential components: a GABAergic evoked inhibitory post-synaptic potentials and a glutamatergic evoked excitatory post-synaptic potentials, respectively. Interleukin-1 increased GABAergic evoked inhibitory post-synaptic potentials amplitudes by 30.3 +/- 3.8% (n = 6) without affecting glutamatergic evoked excitatory post-synaptic potentials. These results support the hypothesis that interleukin-1 inhibitory effects on DRN serotonergic neurons are mediated by an interleukin-1-induced potentiation of evoked GABAergic inhibitory responses.
Subject(s)
Action Potentials/drug effects , Inhibitory Postsynaptic Potentials/drug effects , Interleukin-1/pharmacology , Neurons/drug effects , Raphe Nuclei/cytology , Serotonin/metabolism , gamma-Aminobutyric Acid/metabolism , Animals , Bicuculline/pharmacology , Dose-Response Relationship, Radiation , Drug Interactions , Electric Stimulation/methods , Excitatory Amino Acid Antagonists/pharmacology , GABA Antagonists/pharmacology , In Vitro Techniques , Male , Patch-Clamp Techniques/methods , Rats , Rats, Sprague-Dawley , Serotonin/pharmacologyABSTRACT
Three novel chemically related compounds were studied for their pH-dependent ion channel blocking actions on the transient outward K+ current (I(to)) and the Na+ current (I(Na)) in isolated rat ventricular myocytes. The (+/-)-trans-napthylethoxycyclohexylamines, RSD1108, RSD1070 and RSD1067, showed similar potencies in reducing the inactivation time course of I(to) at pH 7.4. However, RSD1108 (pK(a) 6.8) was a more potent blocker of I(to) at pH 6.4 than the other two compounds (pK(a) values near 8.0). The reduction of inactivation times induced by the RSD compounds was consistent with open channel blockade and in consequence an open channel block model was used in order to estimate blocking and unblocking rate constants. This analysis showed no apparent correlation between pK(a) and onward blocking rate constants for the compounds. However, the unblocking rate constant for the low pK(a) compound RSD1108 at acid pH decreased markedly from that found at normal pH. Both RSD1108 and RSD1070 showed an enhanced potency to block I(Na) at acid pH relative to pH 7.4. However, RSD1108 showed significantly less inhibition of I(Na) at both pH values compared to RSD1070 and RSD1067. Differences in channel block were also evident between RSD1070 and RSD1067, which could be attributed to the difference in napthyl groups between their chemical structures. The compounds exhibited use- and frequency-dependent blockade of I(Na) with the amount of use-dependent blockade greater for RSD1108 and RSD1067 than for RSD1070 at acid pH compared to neutral pH. Greater frequency-dependent inhibition was apparent for RSD1108 as compared to RSD1070 and RSD1067 at both pH 7.4 and 6.4. These results point out the importance of the magnitude of pK(a) and chemical structure in ion channel blocking actions of a series of structurally related compounds.
Subject(s)
Anti-Arrhythmia Agents/pharmacology , Myocardium/metabolism , Potassium Channels/physiology , Sodium Channels/physiology , Animals , Cells, Cultured , Electrophysiology , Heart Ventricles/cytology , Heart Ventricles/drug effects , Heart Ventricles/metabolism , Hydrogen-Ion Concentration , Kinetics , Male , Morpholines/pharmacology , Naphthalenes/pharmacology , Patch-Clamp Techniques , Potassium Channels/drug effects , Rats , Rats, Sprague-Dawley , Sodium Channels/drug effects , Time FactorsABSTRACT
Alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptors are one type of ionotropic glutamate receptor involved in rapid excitatory synaptic transmission. AMPA receptors have been increasingly implicated in long-term potentiation, and recent evidence suggests that they may play a role in disorders affecting the nervous system. The finding that early in postnatal development AMPA receptors are not expressed has lately been the focus of much attention. Resolving the factors involved in AMPA receptor expression suggests that their induction is a developmentally regulated process with the possibility that alterations in receptor expression may be correlated with pathology in neurological disorders. This paper provides an overview of factors involved in AMPA receptor induction as well as of their role in plasticity and neuronal pathologies.
Subject(s)
Neuronal Plasticity/physiology , Neurons/physiology , Receptors, AMPA/physiology , Aging/metabolism , Aging/physiology , Animals , Brain Diseases/metabolism , Humans , Neurons/pathology , Receptors, AMPA/metabolism , SynapsesABSTRACT
The effects of acute application of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNFalpha) on levels of intracellular Ca(2+) ([Ca(2+)]i) and on whole-cell outward and inward K(+) currents were studied in cultured human microglia. TNFalpha elicited a linear increase in [Ca(2+)]i to a plateau level in microglia bathed in either standard physiological saline solution or Ca(2+)-free physiological saline solution. The rate of increase of [Ca(2+)]i or the level of [Ca(2+)]i attained was not significantly altered in the absence of external Ca(2+) indicating that Ca(2+) influx did not contribute appreciably to the cytokine-induced rise in [Ca(2+)]i. This point was directly confirmed using Mn(2+) quenching where no change in signal fluorescence was observed with TNFalpha treatment of microglia in Ca(2+)-free physiological saline solution. The rate of increase of [Ca(2+)]i induced by TNFalpha in Ca(2+)-free physiological saline solution was not altered by prior application of ATP to deplete inositol triphosphate stores indicating that these stores did not contribute to the cytokine response. In whole-cell patch clamp recordings, the acute treatment of human microglia with TNFalpha led to the expression of an outward K(+) current in one-third (14 of 41) of cells. This current was activated at potentials positive to -30 mV, showed rapid kinetics of activation with no evident inactivation and had an I-V relation exhibiting outward rectification. Analysis of tail currents showed reversal of the outward K(+) current near -70 mV and tetraethylammonium (10 mM) inhibited the outward K(+) current to 24% of control level. Acute application of TNFalpha had no effect to alter inward rectifier currents generated from voltage ramps. The signaling pathways involving TNFalpha modulation of [Ca(2+)]i and K(+) channels in human microglia may contribute to functional and pathological actions of the cytokine in the brain.
Subject(s)
Calcium Channels/drug effects , Calcium Signaling/drug effects , Encephalitis/metabolism , Intracellular Fluid/drug effects , Microglia/drug effects , Potassium Channels/drug effects , Tumor Necrosis Factor-alpha/pharmacology , Adenosine Triphosphate/metabolism , Adenosine Triphosphate/pharmacology , Brain/cytology , Brain/metabolism , Brain/physiopathology , Calcium/deficiency , Calcium Channels/metabolism , Calcium Signaling/physiology , Cells, Cultured/drug effects , Cells, Cultured/metabolism , Dose-Response Relationship, Drug , Encephalitis/physiopathology , Enzyme Inhibitors/pharmacology , Fetus , Humans , Indoles/pharmacology , Intracellular Fluid/metabolism , Manganese/pharmacology , Membrane Potentials/drug effects , Membrane Potentials/physiology , Microglia/metabolism , Microscopy, Fluorescence , Patch-Clamp Techniques , Potassium Channels/metabolism , Signal Transduction/drug effects , Signal Transduction/physiology , Spectrometry, Fluorescence , Tetraethylammonium/pharmacologyABSTRACT
Recent work from this laboratory has demonstrated that purinergic-mediated depolarization of human microglia inhibited a store-operated pathway for entry of Ca2+. We have used Fura-2 spectrofluorometry to investigate the effects on store-operated Ca2+ influx induced by replacement of NaCl with Na-gluconate in extracellular solutions. Three separate procedures were used to activate store-operated channels. Platelet activating factor (PAF) was used to generate a sustained influx of Ca2+ in standard physiological saline solution (PSS). The magnitude of this response was depressed by 70% after replacement of PSS with low Cl- PSS. A second procedure used ATP, initially applied in Ca2+-free PSS solution to deplete intracellular stores. The subsequent perfusion of PSS solution containing Ca2+ resulted in a large and sustained entry of Ca2+, which was inhibited by 75% with low Cl- PSS. The SERCA inhibitor cyclopiazonic acid (CPA) was used to directly deplete stores in zero-Ca2+ PSS. Following the introduction of PSS containing Ca2+, a maintained stores-operated influx of Ca2+ was evident which was inhibited by 77% in the presence of the low Cl- PSS. Ca2+ influx was linearly reduced with cell depolarization in elevated K+ (7.5 to 35 mM) suggesting that changes in external Cl- were manifest as altered electrical driving force for Ca2+ entry. However, 50 mM external KCl effectively eliminated divalent entry which may indicate inactivation of this pathway with high magnitudes of depolarization. Patch clamp studies showed low Cl-PSS to cause depolarizing shifts in both holding currents and reversal potentials of currents activated with voltage ramps. The results demonstrate that Cl- channels play an important role in regulating store-operated entry of Ca2+ in human microglia.
Subject(s)
Calcium Signaling , Calcium/metabolism , Ion Channels/physiology , Microglia/metabolism , Adenosine Triphosphate/pharmacology , Anions/metabolism , Buffers , Calcium-Transporting ATPases/antagonists & inhibitors , Cells, Cultured , Embryo, Mammalian , Enzyme Inhibitors/pharmacology , Extracellular Space/metabolism , Gluconates/pharmacology , Humans , Indoles/pharmacology , Patch-Clamp Techniques , Platelet Activating Factor/pharmacology , Potassium/metabolism , Sarcoplasmic Reticulum Calcium-Transporting ATPases , Sodium Chloride/pharmacologyABSTRACT
The effects of the pro-inflammatory cytokine interleukin-1-beta (IL-1beta) on levels of intracellular calcium [Ca(2+)](i) in cultured human microglia have been studied using the fluorescent Ca(2+) indicator fura-2. IL-1beta (2 ng/ml) caused a slow, progressive increase in [Ca(2+)](i) in standard Ca(2+)-containing physiological solution (PSS). A similar effect was observed in separate studies using Ca(2+)-free PSS, however, the mean rate of increase was significantly lower than that measured with PSS. Similar results were obtained in a separate protocol, where cells were exposed to both IL-1beta in Ca(2+)-free PSS and PSS. The slope of the IL-1beta induced increase of [Ca(2+)](i) in Ca(2+)-free PSS was not altered when adenosine triphosphate was added prior to application of the cytokine. These results suggest that IL-1beta-induced responses in human microglia involve both a Ca(2+) entry pathway and a mechanism of intracellular increase other than from IP(3)-sensitive stores.