ABSTRACT
Objective To explore the efficacy ofmicrosurgery and gamma knife treatment on brainstem cavernous malformations.Methods Retrospective analysis was performed on the clinical data of 15 patients with brainstem cavernous malformations treated by microsurgery and 22 patients by gamma knife in our hospital from January 2002 to December 2011.The clinical symptom improvement,scores of Glasgow Outcome Scale,scores of Karnofsky Performance Scale and scores of National Institutes of Health Stroke Scale before and after treatment were observed and compared between the two groups.Results Patients in the micro-surgery group enjoyed the following results:one patient with pontine lesion had residual postoperation; no re-bleeding or death was noted; two had irreversible neurological dysfunction; clinical symptoms improved in 11 patients (73.3％); the postoperative scores of Glasgow Outcome Scale and Karnofsky Performance Scale increased 0.20 and 6.6 as compared with the preoperative ones; The postoperative scores of National Institutes of Health Stroke Scale decreased 1.20 as compared with the preoperative ones.Patients in Gamma Knife group enjoyed the following results:the lesions of two patients increased due to re-bleeding; three had perifocal brain edema; no death was noted;the clinical symptoms improved in 9 patients (40.9％); the postoperative scores of Glasgow Outcome Scale and Karnofsky Performance Scale increased 0.05 and 1.82 as compared with the preoperative ones; the postoperative scores of National Institutes of Health Stroke Scale decreased 0.82 as compared with the preoperative ones.Conclusion Micro-surgery may be more effective than Gamma Knife in treating brainstem cavernous malformations; however,patients with non-surgical conditions or refusing surgery could choose Gamma Knife treatment; gamma knife treatment should be cautiously chosen.
ABSTRACT
Objective To investigate the expression and the role of DCL1 gene in human glioma. Methods Thirty-nine glioma samples and 10 control tissues resected from healthy brain tissue with traumatic brain injury were collected for analysis. Real-time quantitative PCR was employed to detect the DCL1 gene expression at the mRNA level. Plasmid pCS2-DLC1was transfected into the glioma cell line U251 and pCS2-MT was transfected as control group; Western blotting was employed to detect the DCL1 gene expression at the protein level: the expression of Myc; MTT assays were performed to assess the proliferation of glioma cell line U251. Results Glioma tissues showed a significant increased DCL1 gene expression at mRNA level as compared with the control brain tissues (P=0.000). The DLC1 gene expression at mRNA level in the Grade Ⅰ gliomas was obviously higher than that in Grade Ⅱ,Ⅲ or Ⅳ ones (P=0.001). Over-expression of Myc in pCS2-DLC1 transfected group was found, while no obvious Myc expression was noted in the control group. The pCS2-DLC1 transfected group showed higher proliferation level of U251 cell lines than the pCS2-MT control group (P=0.002). Conclusion High expression level of DLC1 might contribute to the formation and development of glioma.