Fast and accurate measurement of taste and smell thresholds using a maximum-likelihood adaptive staircase procedure.

This paper evaluates the use of a maximum-likelihood adaptive staircase psychophysical procedure (ML-PEST), originally developed in vision and audition, for measuring detection thresholds in gustation and olfaction. The basis for the psychophysical measurement of thresholds with the ML-PEST procedure is developed. Then, two experiments and four simulations are reported. In the first experiment, ML-PEST was compared with the Wetherill and Levitt up-down staircase method and with the Cain ascending method of limits in the measurement of butyl alcohol thresholds. The four Monte Carlo simulations compared the three psychophysical procedures. In the second experiment, the test-retest reliability of MLPEST for measuring NaCl and butyl alcohol thresholds was assessed. The results indicate that the ML-PEST method gives reliable and precise threshold measurements. Its ability to detect malingerers shows considerable promise. It is recommended for use in clinical testing.

Thermal hyperalgesia and mechanical allodynia produced by intrathecal administration of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein, gp120.

Astrocytes and microglia in the spinal cord have recently been reported to contribute to the development of peripheral inflammation-induced exaggerated pain states. Both lowering of thermal pain threshold (thermal hyperalgesia) and lowering of response threshold to light tactile stimuli (mechanical allodynia) have been reported. The notion that spinal cord glia are potential mediators of such effects is based on the disruption of these exaggerated pain states by drugs thought to preferentially affect glial function. Activation of astrocytes and microglia can release many of the same substances that are known to mediate thermal hyperalgesia and mechanical allodynia. The aim of the present series of studies was to determine whether exaggerated pain states could also be created in rats by direct, intraspinal immune activation of astrocytes and microglia. The immune stimulus used was peri-spinal (intrathecal, i.t.) application of the Human Immunodeficiency Virus type 1 (HIV-1) envelope glycoprotein, gp120. This portion of HIV-1 is known to bind to and activate microglia and astrocytes. Robust thermal hyperalgesia (tail-flick, TF, and Hargreaves tests) and mechanical allodynia (von Frey and touch-evoked agitation tests) were observed in response to i.t. gp120. Heat denaturing of the complex protein structure of gp120 blocked gp120-induced thermal hyperalgesia. Lastly, both thermal hyperalgesia and mechanical allodynia to i.t. gp120 were blocked by spinal pretreatment with drugs (fluorocitrate and CNI-1493) thought to preferentially disrupt glial function.

Senescent changes in scotopic contrast sensitivity.

Scotopic contrast sensitivity functions (CSFs) were measured for 50 observers between the ages of 20 and 88 years. Using a maximum-likelihood, 2-alternative, temporal forced-choice threshold-estimation algorithm, scotopic CSFs were measured at 7 spatial frequencies ranging from 0.2 to 3.0 cpd, with mean retinal illuminance equated for observers at -0.85 log scotopic Trolands. For each stimulus condition, eight cycles of a horizontal sinusoidal grating were presented within +/- 1 S.D. of a 2-D Gaussian-spatial envelope and within a 1-s Gaussian-temporal envelope. Stimuli were centered on the nasal retina along the horizontal meridian 6 degrees from the fovea. Scotopic CSFs were found to be low-pass. Statistically significant age-related declines in contrast sensitivities were found for spatial frequencies at or below 1.2 cpd. There was also a statistically significant decrease in the high frequency cut-off with age (P < 0.01). An explanation of these results in terms of optical factors is rejected, while the results are consistent with age-related changes in the magnocellular pathway.

Efficient estimation of sensory thresholds with ML-PEST.

A set of C and C+2 routines are described that allow the efficient estimation of sensory thresholds in psychophysical experiments using a maximum-likelihood staircase procedure. They have been used effectively in visual, auditory, gustatory, and olfactory psychophysics.

Cortical magnification theory fails to predict visual recognition.

The sense of form is poor in indirect view. Yet the cortical magnification theory asserts that the disadvantage can be made up by scaling the image size according to the spatial variation in the mapping of the retina onto the cortex. It is thus assumed that all visual information passes through a functionally homogeneous neural circuitry, with the spatial sampling of input signals varying across the visual field. We challenge this notion by showing that character recognition in the visual field cannot be accommodated by any concept of sole size scaling but requires increasing both size and contrast of the target being viewed. This finding is formalized into a hyperbolic law which states that target size multiplied by log contrast is constant across the visual field. We conclude that the scalar cortical magnification theory fails for character recognition since the latter depends on multidimensional pattern representations in higher, i.e. striate and prestriate, cortical areas.

Contrast thresholds for identification of numeric characters in direct and eccentric view.

Aubert and Foerster (1857) are frequently cited for having shown that the lower visual acuity of peripheral vision can be compensated for by increasing stimulus size. This result is seemingly consistent with the concept of cortical magnification, and it has been confirmed by many subsequent authors. Yet it is rarely noted that Aubert and Foerster also observed a loss of the "quality of form." We have studied the recognition of numeric characters in foveal and eccentric vision by determining the contrast required for 67% correct identification. At each eccentricity, the lowest contrast threshold is achieved with a specific stimulus size. But the contrast thresholds for these optimal stimuli are not independent of retinal eccentricity as cortical magnification scaling would predict. With high-contrast targets, however, threshold target sizes were consistent with cortical magnification out to 6 degrees eccentricity. Beyond 6 degrees, threshold target sizes were larger than cortical magnification predicted. We also investigated recognition performance in the presence of neighboring characters (crowding phenomenon). Target character size, distance of flanking characters, and precision of focusing of attention all affect recognition. The influence of these parameters is different in the fovea and in the periphery. Our findings confirm Aubert and Foester's original observation of a qualitative difference between foveal and peripheral vision.

Visual masking at different polar angles in the two-dimensional Fourier plane.

Human contrast thresholds were measured at 86 points in the two-dimensional Fourier plane with and without masks. The test stimuli were sinusoidal gratings in a 2.55-deg circular field. The superimposed masks were sinusoidal gratings having a polar spatial frequency of 8 cycles/deg, a contrast of 0.31, and one of five polar angles: 90, 105, 120, 135, and 180 deg. The test grating contrast for 75.5% correct detection in a three-alternative, forced-choice paradigm was determined by a maximum-likelihood adaptive psychophysical procedure. Three other observers were tested on subsets of these conditions. The threshold elevation surfaces produced by the masks lead to the following conclusions: spatial-frequency bandwidth is independent of mask orientation and has a value of approximately 2 octaves, orientation bandwidth is wider for oblique masks than for horizontal and vertical masks, the principle of spectral polar separability is violated, and two-dimensional Gabor functions in the frequency domain account for the masking effects. Individual differences were found among the observers.

Spatial frequencies and the cerebral hemispheres: contrast sensitivity, visible persistence, and letter classification.

The hypothesis that the two cerebral hemispheres are specialized for processing different visual spatial frequencies was investigated in three experiments. No differences between the left and right visual fields were found for: (1) contrast-sensitivity functions measured binocularly with vertical gratings ranging from 0.5 to 12 cycles per degree (cpd); (2) visible persistence durations for 1- and 10-cpd gratings measured with a stimulus alternation method; and (3) accuracy (d') and reaction times to correctly identify digitally filtered letters as targets (L or H) or nontargets (T or F). One significant difference, however, was found: In Experiment 3, a higher decision criterion (beta) was used when filtered letters were identified in the right visual field than when they were identified in the left. The letters were filtered with annular, 1-octave band-pass filters with center spatial frequencies of 1, 2, 4, 8, and 16 cpd. Combining four center frequencies with three letter sizes (0.5 degrees, 1 degree, and 2 degrees high) made some stimuli equivalent in distal spatial frequency (cycles per object) and some equivalent in proximal spatial frequency (cycles per degree). The effective stimulus in the third experiment seemed to be proximal spatial frequency (cycles per degree) not distal (cycles per object). We conclude that each cerebral hemisphere processes visual spatial frequency information with equal accuracy but that different decision rules are used.

Identification confusions among letters of the alphabet.

Black, uppercase letters, subtending 6.0' of arc in height, were presented tachistoscopically to 6 subjects. An exposure duration was chosen to keep the subject's identification performance at about 50% correct. On each trial a single letter was presented, and the subject was required to identify the letter by verbal response. The resulting 26 X 26 confusion matrix was based on 3,900 trials (150 trials per letter). Several models of visual processing were used to generate predicted confusions among letter pairs. Models based on template overlap, geometric features, and two-dimensional spatial frequency content (Fourier transforms) were tested. The highest correlation (.70) between actual and predicted confusions was attained by the model based on the Fourier transformed letters filtered by the human contrast sensitivity function. These results demonstrate that the spatial frequency content of visual patterns can provide a valuable metric for predicting their psychological similarity. The results further suggest that spatial frequency models of visual processing are competitive with feature analysis models.

Hemispheric specialization for the processing of linguistic and non-linguistic stimuli in congenitally deaf and hearing adults: a review and contribution.

Congenitally deaf individuals, interpreters for the deaf and hearing controls viewed words, dots, American sign language signs and drawings of objects presented tachistoscopically. The stimuli were presented both bilaterally and unilaterally to the subjects. The deaf subjects showed a significant left visual field (LVF) superiority for the processing of the drawings while the nondeaf groups showed significant right visual field (RVF) advantages. Laterality differences between groups on the other tasks were nonsignificant. The deaf group also showed a significant LVF advantage in the bilateral condition across all tasks as opposed to a RVF advantage yielded by nondeaf groups. A significant LVF advantage for words and dots was found compared to the RVF advantage for signs across all subjects. The differences between deaf and nondeaf groups are discussed in terms of processing strategies and the critical period for language acquisition theory.

Internal representation of visual texture as the basis for the judgment of similarity.

The relationship between Fourier spectra of visual textures (represented both by the actual frequency components and by the response of four hypothetical channels selectively sensitive to spatial frequency) and the perceptual appearance of the textures was investigated. Thirty textures were synthesized by combining seven spatial frequencies whose amplitudes were randomly chosen and then scaled to give an overall contrast of .9. Similarity judgments were collected using both the method of triadic comparison (two subjects, 4,060 trials each) and the method of paired comparison (six subjects, 435 trials each). The similarity judgments were subjected to MDSCAL and INDSCAL dimensions were found to be optimally oriented in terms of spatial frequency information without rotation. The seven spatial frequency components accounted for 90.6% of the variance in the 3-D INDSCAL space, while the four channels accounted for 91.8% of the variance in the first two dimensions. The data further suggest that the four channels may interact in an opponent process manner. The results support the idea that the visual internal representation of stimuli is based on spatial frequency analysis rather than feature extraction.