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Nature ; 416(6881): 601-2; discussion 600, 602, 2002 Apr 11.
Article in English | MEDLINE | ID: mdl-11935145

ABSTRACT

Quist and Chapela's conclusion that the transgenes they claim to have detected in native maize in Oaxaca, Mexico, are predominantly reassorted and inserted into a "diversity of genomic contexts" seems to be based on an artefact arising from the inverse polymerase chain reaction (i-PCR) they used to amplify sequences flanking 35S transgenes from cauliflower mosaic virus (CaMV).


Subject(s)
Polymerase Chain Reaction/standards , Retroelements/genetics , Transgenes/genetics , Zea mays/genetics , Artifacts , DNA Primers/genetics , Genome, Plant , Introns/genetics , Mexico , Nucleic Acid Hybridization , Plants, Genetically Modified , Polymerase Chain Reaction/methods , Recombination, Genetic/genetics , Reproducibility of Results
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