ABSTRACT
To determine the incidence of rotavirus infection among dairy herds in the State of São Paulo, Brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. Polyacrylamide gel electrophoresis (PAGE) identified 28 samples positive for group A rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. Electron microscopy revealed spherical virus particles with a diameter of 37 nm and without a defined surface structure. The present study is the first report of a bisegmented virus identified in cattle in Brazil.
Subject(s)
Cattle Diseases/virology , Picobirnavirus/isolation & purification , RNA, Double-Stranded/isolation & purification , Animals , Brazil , Cattle , Diarrhea/veterinary , Diarrhea/virology , Electrophoresis, Polyacrylamide Gel/veterinary , Feces/virology , Microscopy, ElectronABSTRACT
An outbreak of severe gastroenteritis affecting 132 adults and children occurred in the small city of Mirassol, São Paulo, Brazil, in 1992. The outbreak of diarrhoeal disease had an abrupt onset and afflicted all age segments of the population. Group A rotavirus was the only pathogen associated with the epidemic. It was detected in 12 of the 27 (44%) stool specimens analyzed and was identified as serotype G2 rotavirus. Severe dehydration was common among adults and older children, and 35% of all the notified cases were hospitalized for parenteral rehydration. Contamination of the main water supply was the most likely source.