Coordination ligand exchange of a xanthene probe-Ce(III) complex for selective fluorescence sensing of inorganic pyrophosphate.

A fluorescence sensing system for inorganic pyrophosphate based on ligand exchange of the Ce(III) complex of a xanthene-type probe is developed. This sensing system is successfully applied to the fluorescence detection of polymerase-catalyzed DNA amplification using loop-mediated isothermal amplification.