ABSTRACT
BACKGROUND: The aim of this study was to evaluate the concentration of vascular endothelial growth factor (VEGF) in follicular fluid and in granulosa cell cultures in relation to the degree of apoptosis in granulosa cells from patients with different types of ovarian response to controlled ovarian hyperstimulation. METHODS: We studied 30 women who underwent controlled ovarian hyperstimulation and oocyte retrieval. Group A comprised patients with 1-4 follicles (n = 10), group B patients with 5-14 follicles (n = 10) and group C patients with >15 follicles (n = 10). RESULTS: Mean (+/-SD) VEGF concentrations in follicular fluid were 1232 +/- 209, 813 +/- 198 and 396 +/- 103 pg/ml for groups A, B and C respectively (P > 0.01). Concentrations of VEGF in granulosa cell supernatant were 684 +/- 316, 1101 +/- 295 and 1596 +/- 227 pg/ml respectively (P < 0.05). Percentages of apoptotic cells in granulosa cells culture was 55.02 +/- 7.5, 23.98 +/- 4.4 and 14.2 +/- 2.3% respectively (A versus B, P < 0.01, A versus C, P < 0.006, B versus C, NS). CONCLUSIONS: Our findings showed that in patients with decreased ovarian response to controlled ovarian hyperstimulation, follicular fluid VEGF concentration is elevated, the concentration from granulosa cells culture supernatant is decreased and the percentage of apoptotic granulosa cells is increased, while opposite findings occurred in patients with normal or hyper-responses.
Subject(s)
Endothelial Growth Factors/metabolism , Granulosa Cells/physiology , Lymphokines/metabolism , Ovary/physiopathology , Ovulation Induction , Adult , Cells, Cultured , Female , Fertilization in Vitro , Follicular Fluid/metabolism , Humans , Osmolar Concentration , Ovarian Follicle/pathology , Ovary/pathology , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth FactorsSubject(s)
Humans , Female , Adult , Middle Aged , Echocardiography, Doppler , Genital Neoplasms, Female , Neoplasms , UltrasonographySubject(s)
Humans , Female , Adolescent , Adult , Middle Aged , Age Factors , Anal Canal/physiology , Manometry , Pressure/adverse effects , Rectum/abnormalitiesSubject(s)
Humans , Female , Adult , Middle Aged , Diagnostic Errors , Genital Diseases, Female , Genital Neoplasms, Female/pathology , OvarySubject(s)
Humans , Female , Adult , Middle Aged , Urethra/physiopathology , Urinary Incontinence , Urinary Tract Infections/etiologySubject(s)
Humans , Female , Adult , Chromosome Aberrations/diagnosis , Cytogenetics , Infertility/geneticsSubject(s)
Humans , Female , Adult , Middle Aged , Ultrasonography , Echocardiography, Doppler , Neoplasms , Genital Neoplasms, FemaleSubject(s)
Humans , Female , Adolescent , Adult , Middle Aged , Anal Canal/physiology , Pressure/adverse effects , Age Factors , Manometry/methods , Rectum/abnormalitiesSubject(s)
Humans , Female , Adult , Middle Aged , Genital Neoplasms, Female/pathology , Genital Diseases, Female , Ovary , Diagnostic ErrorsSubject(s)
Humans , Female , Adult , Middle Aged , Urethra/physiopathology , Urinary Incontinence , Urinary Tract Infections/etiologySubject(s)
Humans , Female , Adult , Infertility/genetics , Chromosome Aberrations/diagnosis , CytogeneticsABSTRACT
El deterioro de la función de los músculos del piso de la pelvis se incrementa con la edad y se evidencia en las mujeres fundamentalmente durante la posmenopausia, siendo un factor etiopatogénico de gran importancia
Subject(s)
Female , Adult , Abdominal Muscles , Pelvic Floor , Receptors, EstrogenABSTRACT
El deterioro de la función de los músculos del piso de la pelvis se incrementa con la edad y se evidencia en las mujeres fundamentalmente durante la posmenopausia, siendo un factor etiopatogénico de gran importancia