[Genetic transformation of flax (Linum usitatissimum L.) with chimeric GFP-TUA6 gene for visualisation of microtubules].

The data of Agrobacterium-mediated transformation of some Linum usitatissimum cultivars zoned on the territories of Belarus and Ukraine with the plasmid carrying chimeric GFP-TUA6 gene and nptII gene as selectable marker conferring resistance to kanamycin are presented in this study. Transformation was affected by a number of factors including optical density (OD600), time of inoculation of explants with Agrobacterium and co-culture conditions. Transgenic nature of obtained lines was confirmed by PCR analysis. Expression of GFP-TUA6 gene was detected with confocal laser scanning microscopy. The obtained transgenic lines can be used for further functional studies the role of microtubules in the processes of building the flax fibres and resistance to wind.

[Biodiesel from microalgae: ways of increasing effectiveness of lipids accumulation by genetic engineering methods].

Microalgae are viewed as one of the most perspective producer of lipids for biodiesel production. The review shows the results of researches of genes' expression increase actually included in fatty acids biosynthesis. The increase of effectiveness of solar energy absorption and carbon dioxide fixation influences the microalgae productivity. Blocking expression of genes that are responsible for starch synthesis, changes the balance towards the quantity growth of lipids in the cell. The change of the length in fatty acids carbon backbone chain towards its shortening is important in the technology of biodiesel production. Operating processes of lipids' catabolism is another way of increasing their quantity. And at last using the methods of transcription analysis allows us to get deeper into the process of intensive accumulation of lipids in stressful conditions for the purpose of directing these processes.

[Developing transgenic barley lines producing human lactoferrin using mutant alfa-tubulin gene as selective marker gene].

Method of biolistic transformation was used for genetic improvement of commercial barley cultivars (Oksamitoviy, Vodogray and Getman). The plasmid pHLFTuBA was used for particle bombardment that consists of the hLF gene under the control of the barley glutelin B-1 promoter and a selectable marker gene, alpha-tubulin conferring resistance to trifluralin (dinitroanilinr herbicide). Preliminary screening of different trifluralin concentration range from 0,1 to 30 microM was tested for determination of effective selective agent concentration. Two transgenic barley line of genotype Oksamitiviy and transgenic callus line of cultivar Getman were obtained after selection on 10 microM of trifluralin. To confirm the transgenic nature of regenerated plants, the PCR analysis was carried out. The 734bp length fragment of hLFgene was amplified from both regenerated plants.