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1.
Cell Host Microbe ; 17(5): 628-41, 2015 May 13.
Article in English | MEDLINE | ID: mdl-25891357

ABSTRACT

The differentiation and protective capacity of Plasmodium-specific T cells are regulated by both positive and negative signals during malaria, but the molecular and cellular details remain poorly defined. Here we show that malaria patients and Plasmodium-infected rodents exhibit atypical expression of the co-stimulatory receptor OX40 on CD4 T cells and that therapeutic enhancement of OX40 signaling enhances helper CD4 T cell activity, humoral immunity, and parasite clearance in rodents. However, these beneficial effects of OX40 signaling are abrogated following coordinate blockade of PD-1 co-inhibitory pathways, which are also upregulated during malaria and associated with elevated parasitemia. Co-administration of biologics blocking PD-1 and promoting OX40 signaling induces excessive interferon-gamma that directly limits helper T cell-mediated support of humoral immunity and decreases parasite control. Our results show that targeting OX40 can enhance Plasmodium control and that crosstalk between co-inhibitory and co-stimulatory pathways in pathogen-specific CD4 T cells can impact pathogen clearance.


Subject(s)
Cell Differentiation , Immunity, Humoral , Malaria/immunology , Plasmodium/immunology , Programmed Cell Death 1 Receptor/metabolism , Receptors, OX40/metabolism , T-Lymphocytes, Helper-Inducer/physiology , Animals , Gene Expression Regulation , Humans , Mice
2.
Curr Opin Immunol ; 34: 68-74, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25726751

ABSTRACT

Following infection, naïve CD4 T cells can differentiate into various functionally distinct effector and memory subsets, including T follicular helper (TFH) cells that orchestrate germinal center (GC) reactions necessary for high-affinity, pathogen-specific antibody responses. The origins and function of this cell type have been extensively examined in response to subunit immunization with model antigens. More recently, we are beginning to also appreciate the extent to which microbial infections shape the generation, function and maintenance of TFH cells. Here, we review recent advances and highlight additional knowledge gaps in our understanding of how microbial infections influence priming, differentiation, localization and activity of TFH cells following acute and chronic infections.


Subject(s)
Infections/immunology , T-Lymphocytes, Helper-Inducer/cytology , T-Lymphocytes, Helper-Inducer/immunology , Animals , B-Lymphocytes/immunology , Cell Differentiation , Germinal Center/cytology , Germinal Center/immunology , Humans , Immunologic Memory
3.
Blood ; 125(12): 1957-67, 2015 Mar 19.
Article in English | MEDLINE | ID: mdl-25538045

ABSTRACT

Sp1 and Sp3 belong to the specificity proteins (Sp)/Krüppel-like transcription factor family. They are closely related, ubiquitously expressed, and recognize G-rich DNA motifs. They are thought to regulate generic processes such as cell-cycle and growth control, metabolic pathways, and apoptosis. Ablation of Sp1 or Sp3 in mice is lethal, and combined haploinsufficiency results in hematopoietic defects during the fetal stages. Here, we show that in adult mice, conditional pan-hematopoietic (Mx1-Cre) ablation of either Sp1 or Sp3 has minimal impact on hematopoiesis, whereas the simultaneous loss of Sp1 and Sp3 results in severe macrothrombocytopenia. This occurs in a cell-autonomous manner as shown by megakaryocyte-specific (Pf4-Cre) double-knockout mice. We employed flow cytometry, cell culture, and electron microscopy and show that although megakaryocyte numbers are normal in bone marrow and spleen, they display a less compact demarcation membrane system and a striking inability to form proplatelets. Through megakaryocyte transcriptomics and platelet proteomics, we identified several cytoskeleton-related proteins and downstream effector kinases, including Mylk, that were downregulated upon Sp1/Sp3 depletion, providing an explanation for the observed defects in megakaryopoiesis. Supporting this notion, selective Mylk inhibition by ML7 affected proplatelet formation and stabilization and resulted in defective ITAM receptor-mediated platelet aggregation.


Subject(s)
Blood Platelets/cytology , Megakaryocytes/cytology , Sp1 Transcription Factor/genetics , Sp3 Transcription Factor/genetics , Animals , Azepines/chemistry , Blood Platelets/metabolism , Bone Marrow/metabolism , Flow Cytometry , Lectins, C-Type/metabolism , Mice , Mice, Knockout , Naphthalenes/chemistry , Platelet Aggregation , Platelet Membrane Glycoproteins/metabolism , Proteome , Signal Transduction , Sp1 Transcription Factor/metabolism , Sp3 Transcription Factor/metabolism , Spleen/metabolism , Thrombocytopenia/metabolism , Transcription Factors/metabolism
4.
Development ; 141(12): 2391-401, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24850855

ABSTRACT

Mammalian development is regulated by the interplay of tissue-specific and ubiquitously expressed transcription factors, such as Sp1. Sp1 knockout mice die in utero with multiple phenotypic aberrations, but the underlying molecular mechanism of this differentiation failure has been elusive. Here, we have used conditional knockout mice as well as the differentiation of mouse ES cells as a model with which to address this issue. To this end, we examined differentiation potential, global gene expression patterns and Sp1 target regions in Sp1 wild-type and Sp1-deficient cells representing different stages of hematopoiesis. Sp1(-/-) cells progress through most embryonic stages of blood cell development but cannot complete terminal differentiation. This failure to fully differentiate is not seen when Sp1 is knocked out at later developmental stages. For most Sp1 target and non-target genes, gene expression is unaffected by Sp1 inactivation. However, Cdx genes and multiple Hox genes are stage-specific targets of Sp1 and are downregulated at an early stage. As a consequence, expression of genes involved in hematopoietic specification is progressively deregulated. Our work demonstrates that the early absence of active Sp1 sets a cascade in motion that culminates in a failure of terminal hematopoietic differentiation and emphasizes the role of ubiquitously expressed transcription factors for tissue-specific gene regulation. In addition, our global side-by-side analysis of the response of the transcriptional network to perturbation sheds a new light on the regulatory hierarchy of hematopoietic specification.


Subject(s)
Hematopoiesis , Hematopoietic Stem Cells/cytology , Sp1 Transcription Factor/physiology , Animals , Bone Marrow Cells/cytology , Cell Differentiation , Cell Lineage , Embryonic Stem Cells/cytology , Gene Expression Profiling , Gene Expression Regulation, Developmental , Gene Regulatory Networks , Macrophages/cytology , Mice , Mice, Knockout , Oligonucleotide Array Sequence Analysis , Phenotype , Protein Binding , Stem Cells/cytology
5.
Mol Cell Biol ; 33(19): 3879-92, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23897431

ABSTRACT

Rad23a and Rad23b proteins are linked to nucleotide excision DNA repair (NER) via association with the DNA damage recognition protein xeroderma pigmentosum group C (XPC) are and known to be implicated in protein turnover by the 26S proteasome. Rad23b-null mice are NER proficient, likely due to the redundant function of the Rad23b paralogue, Rad23a. However, Rad23b-null midgestation embryos are anemic, and most embryos die before birth. Using an unbiased proteomics approach, we found that the majority of Rad23b-interacting partners are associated with the ubiquitin-proteasome system (UPS). We tested the requirement for Rad23b-dependent UPS activity in cellular proliferation and more specifically in the process of erythropoiesis. In cultured fibroblasts derived from embryos lacking Rad23b, proliferation rates were reduced. In fetal livers of Rad23b-null embryos, we observed reduced proliferation, accumulation of early erythroid progenitors, and a block during erythroid maturation. In primary wild-type (WT) erythroid cells, knockdown of Rad23b or chemical inhibition of the proteasome reduced survival and differentiation capability. Finally, the defects linked to Rad23b loss specifically affected fetal definitive erythropoiesis and stress erythropoiesis in adult mice. Together, these data indicate a previously unappreciated requirement for Rad23b and the UPS in regulation of proliferation in different cell types.


Subject(s)
Cell Proliferation , DNA-Binding Proteins/genetics , Erythropoiesis/genetics , Proteasome Endopeptidase Complex/genetics , Animals , Blotting, Western , Cell Differentiation/genetics , Cells, Cultured , DNA-Binding Proteins/metabolism , Embryo, Mammalian/cytology , Embryo, Mammalian/metabolism , Erythrocytes/cytology , Erythrocytes/metabolism , Erythroid Precursor Cells/cytology , Erythroid Precursor Cells/metabolism , Female , Fibroblasts/cytology , Fibroblasts/metabolism , Flow Cytometry , Liver/cytology , Liver/embryology , Liver/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Proteasome Endopeptidase Complex/metabolism , Protein Binding , RNA Interference
6.
Exp Hematol ; 39(7): 730-40.e1-2, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21549805

ABSTRACT

OBJECTIVE: The transcription factor PAX5 is essential for the activation of B-cell-specific genes and for the silencing of myeloid-specific genes. We previously determined the molecular mechanism by which PAX5 silences the myeloid-specific colony-stimulating-factor-receptor (Csf1R) gene and showed that PAX5 directly binds to the Csf1r promoter as well as to an intronic enhancer that generates an antisense transcript in B cells. Here we examine the role of PAX5 in the regulation of sense and antisense transcription in B cells. MATERIALS AND METHODS: We performed PAX5-specific chromatin immunoprecipitation analyses across the Csfr1 locus. We investigated the role of PAX5 in regulating Csf1r sense and antisense promoter activity by transient transfections and by employing a Pax5(-/-) pro-B-cell line expressing an inducible PAX5 protein. PAX5 interacting factors were identified by pull-down experiments. The role of the transcription factor Sp3 in driving antisense promoter expression was examined in B cells from Sp3 knockout mice. RESULTS: PAX5 differentially regulates the Csf1r promoter and the promoter of the antisense transcript. PAX5 interferes with PU.1 transactivation at the sense promoter by binding to a PAX5 consensus sequence. At the antisense promoter, PAX5 does not specifically recognize DNA, but interacts with Sp3 to upregulate antisense promoter activity. Antisense promoter activation by PAX5 is dependent on the presence of its partial homeo-domain. CONCLUSIONS: We demonstrate that PAX5 regulates Csf1r in B cells by reducing the frequency of binding of the basal transcription machinery to the promoter and by activating antisense RNA expression.


Subject(s)
B-Lymphocytes/metabolism , Gene Expression Regulation , PAX5 Transcription Factor/genetics , Promoter Regions, Genetic/genetics , Receptor, Macrophage Colony-Stimulating Factor/genetics , Animals , Base Sequence , Binding Sites/genetics , Cell Line , Cell Line, Tumor , Cells, Cultured , Chromatin Immunoprecipitation , DNA, Antisense/genetics , Mice , Mice, Knockout , Mutation , PAX5 Transcription Factor/metabolism , Protein Binding , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/metabolism , Sp3 Transcription Factor/genetics , Sp3 Transcription Factor/metabolism , Trans-Activators/genetics , Trans-Activators/metabolism
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