Ageing and its consequences - the use of virtual reality (VR) as a tool to visualize the problems of the elderly.

BACKGROUND: The population ageing phenomenon is mainly attributable to the increasing proportion of people >60 years of age. This demographic situation requires more attention to the needs of the elderly. Given the lack of effective methods to expand the knowledge of and increase sensitivity to elderly people's problems, especially among the younger generation, an attempt has been made to use virtual reality (VR) to prepare an educational tool focused on these problems. This paper will focus on the use of VR in depicting fundamental problems in the daily live of older people. MATERIAL AND METHODS: For project purposes, a questionnaire (26 question) was developed, concerning data on socio-demographic situation, health and physical conditions; social, living and housing support needs. Additionally, qualitative interviews were conducted with elderly people regarding their needs about housing arrangements and daily activities. It was distributed to 100 people, 34 completed questionnaires were obtained from 30 women and 4 men. The study results were intended as information that would provide the basis for developing VR scenarios, not for the epidemiological purposes. RESULTS: The mean age of the respondents was 73.7±7.5 (60‒86 years). Different situations, as heavy housework, lifting/carrying heavy objects, lifting hands above the shoulder level, bending and squatting were identified that pose problems for elderly people. Based on these results, 3 scenario proposals were prepared, including situations: in the bathroom, in the supermarket, on a trip to the city. These scenarios were used to prepare the exercises in VR in the frame of European project - the Mixed Reality on Universal Design's Secret Service (Mr. UD) Project. CONCLUSIONS: The use of immersive technology such as VR can be beneficial for young people to be able to see perspectives of the elderly. Students from various fields (medicine, nursing, future engineers, designers, sociologists, etc.) may feel like the elderly and experience their daily challenges. Med Pr. 2023;74(3):159-70.

Inclusion and adaptation beyond disability: Using virtual reality to foster empathy.

BACKGROUND: Virtual reality (VR) has the potential to be a powerful tool in promoting empathy towards inclusion, particularly for individuals with impairments such as mobility difficulties, vision deficits, or autism but also about pregnancy, which can create temporary difficulties. By immersing users in simulated environments that replicate the experiences of those with different abilities, VR can create a sense of understanding and empathy for those who face challenges in their daily lives. For example, VR experiences can simulate the experience of navigating space as someone with a mobility impairment, providing a new perspective and appreciation for the difficulties that others face. Similarly, VR experiences can simulate the experience of vision impairment, pregnancy, or autism, providing a window into the challenges faced by those with these conditions and fostering empathy and understanding. MATERIAL AND METHODS: During the development of this study, field experts were consulted to ensure the robustness of the methods employed. Then, questionnaires were specifically developed to explore disabilities and challenges related to inclusion and were administered to a large population. Additionally, guided interviews were conducted with individuals who possess specific impairments to gather first-hand insights. RESULTS: The results obtained from the questionnaires and interviews provide a comprehensive overview of the inclusion challenges that necessitate attention and resolution. By drawing on the expertise of both experts and individuals with lived experiences, a holistic landscape of inclusion challenges has been established. CONCLUSIONS: The VR emerges as a powerful tool for promoting inclusion and fostering understanding among individuals. Its capacity to create immersive experiences that facilitate empathy has the potential to reshape society into a more compassionate and empathetic one. By leveraging the unique capabilities of VR, we can bridge the gap between different perspectives, fostering greater understanding, acceptance, and inclusivity. Med Pr. 2023;74(3):171-85.

Increasing awareness and empathy among university students through immersive exercises - testing of the virtual reality application: A pilot study.

BACKGROUND: This paper presents an overview of a pilot study focused on testing the effectiveness of immersive virtual reality (VR) exercises, within extended reality experiences, in increasing awareness and empathy among university students towards pregnant women, elderly people, people in wheelchairs, and people with some sort of sight impairment. The extended reality experience was designed to simulate various scenarios that reflect the experiences of people from diverse backgrounds, to promote a better understanding of different perspectives and social issues related to some of the challenges tackled by people whose limitations are many times overlooked. MATERIAL AND METHODS: A design-based research methodology was applied and qualitative and quantitative data were collected in samples of 20 students from 3 countries. RESULTS: Overall, the results suggest that immersive VR applications can be an effective tool in increasing awareness and empathy among higher education students. The use of VR technology can create a sense of presence and immersion that allows students to experience situations that they may not have encountered otherwise. The results attained with the immersive experiences have provided evidence that these solutions can foster a greater understanding of different perspectives and promote empathy towards individuals from diverse backgrounds. CONCLUSIONS: Ongoing research correlated with the Mixed Reality on Universal Design's Secret Service (Mr. UD) project results is already expanding on these findings by testing the effectiveness of VR applications in different contexts and with larger and more diverse samples. Additionally, the research conducted has provided relevant evidence that suggests that VR applications and their inclusion in training programs may help promote behavior change and reduce prejudice and discrimination towards marginalized groups. Med Pr. 2023;74(3):187-97.

The Identity and Mineral Composition of Natural, Plant-Derived and Artificial Sweeteners.

The qualitative X-ray phase analysis of natural and artificial food sweeteners was applied to trace the authenticity of such food additives. The mineral composition of different sweeteners commonly added to foods was studied to estimate their mineral profiles and assess the risk related to the toxic elements intake caused by sweetener consumption. The concentration of twenty elements (Ag, Al, B, Ba, Bi, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, Sr, Ti, V, and Zn) was measured using the inductively coupled plasma-optical emission spectroscopy (ICP-OES) method after the representative samples were wet-digested with a concentrated nitric acid and hydrogen peroxide mixture in a closed-vessel microwave-assisted system. Differences between the mineral compositions of the examined sweeteners were statistically evaluated and discussed. The relationships between the concentrations of the elements determined in the analyzed sweeteners were also investigated. The successful application of the X-ray powder diffraction method proved the identity of all investigated sweeteners; all the analyzed products contained the expected sweetening agent. The results of the quantification of all the elements in the examined sweeteners indicated that these products cannot be considered nutritionally dense. Hence, the presence of toxic elements like Cd, Cr, Ni, and Pb distinctly indicates the need to test such products to guarantee their quality and ensure consumer safety.

Catalytic reductions of nitroaromatic compounds over heterogeneous catalysts with rhenium sub-nanostructures.

Nitroaromatic compounds (NACs) are key contaminants of anthropogenic origin and pose a severe threat to human and animal lives. Although the catalytic activities of Re nanostructures (NSs) are significantly higher than those of other heterogeneous catalysts containing NSs, few studies have been reported on the application of Re-based nanocatalysts for NAC hydrogenation. Accordingly, herein, catalytic reductions of nitrobenzene (NB), 4-nitrophenol (4-NP), 2-nitroaniline (2-NA), 4-nitroaniline (4-NA), and 2,4,6-trinitrophenol (2,4,6-TNP) over new Re-based heterogeneous catalysts were proposed. The catalytic materials were designed to enable effective syntheses and stabilisation of particularly small Re structures over them. Accordingly, catalytic hydrogenations of NACs under mild conditions were significantly enhanced by Re sub-nanostructures (Re-sub-NSs). The highest pseudo-first-order rate constants for NB, 4-NP, 2-NA, 4-NA, and 2,4,6-TNP reductions over the catalyst acquired by stabilising Re using bis(3-aminopropyl)amine (BAPA), which led to Re-sub-NSs with Re concentrations of 16.7 wt%, were 0.210, 0.130, 0.100, 0.180, and 0.090 min-1, respectively.

Green and Effective Preparation of α-Hydroxyphosphonates by Ecocatalysis.

A green and effective approach for the synthesis of structurally diversed α-hydroxyphosphonates via hydrophosphonylation of aldehydes under solventless conditions and promoted by biosourced catalysts, called ecocatalysts "Eco-MgZnOx" is presented. Ecocatalysts were prepared from Zn-hyperaccumulating plant species Arabidopsis halleri, with simple and benign thermal treatment of leaves rich in Zn, and without any further chemical treatment. The elemental composition and structure of Eco-MgZnOx were characterized by MP-AES, XRPD, HRTEM, and STEM-EDX techniques. These analyses revealed a natural richness in two unusual and valuable mixed zinc-magnesium and iron-magnesium oxides. The ecocatalysts were employed in this study to demonstrate their potential use in hydrophosphonylation of aldehydes, leading to various α-hydroxyphosphonate derivatives, which are critical building blocks in the modern chemical industry. Computational chemistry was performed to help discriminate the role of some of the constituents of the mixed oxide ecocatalysts. High conversions, broad substrate scope, mild reaction conditions, and easy purification of the final products together with simplicity of the preparation of the ecocatalysts are the major advantages of the presented protocol. Additionally, Eco-MgZnOx-P could be recovered and reused for up to five times.

Usability Testing of Virtual Reality Applications-The Pilot Study.

The need for objective data-driven usability testing of VR applications is becoming more tangible with the rapid development of numerous VR applications and their increased accessibility. Traditional methods of testing are too time and resource consuming and might provide results that are highly subjective. Thus, the aim of this article is to explore the possibility of automation of usability testing of VR applications by using objective features such as HMD built-in head and hands tracking, EEG sensor, video recording, and other measurable parameters in addition to automated analysis of subjective data provided in questionnaires. For this purpose, a simple VR application was created which comprised relatively easy tasks that did not generate stress for the users. Fourteen volunteers took part in the study and their signals were monitored to acquire objective automated data. At the same time the observer was taking notes of subjects' behaviour, and their subjective opinions about the experience were recorded in a post-experiment questionnaire. The results acquired from signal monitoring and questionnaires were juxtaposed with observation and post-interview results to confirm the validity and efficacy of automated usability testing. The results were very promising, proving that automated usability testing of VR applications is potentially achievable.

Micro- and Nanoparticulate Hydroxyapatite Powders as Fillers in Polyacrylate Bone Cement-A Comparative Study.

Acrylate polymer-based bone cements constitute the most popular bonding agents used in regenerative surgery. Due to their inferior biocompatibility, however, these materials are often enriched with ceramic additives including hydroxyapatite (HAp). The aim of this paper was to perform a comparative study of the acrylate cements filled with different content (3-21%) of nano- and microscale hydroxyapatite. The work concerns a comparison of times and temperatures of the cross-linking reaction, as well as morphology, glass transition temperature, and principal mechanical properties of the resulting composites. Before being used as a filler, both HAp forms were subjected to an in-depth characterization of their morphology, specific surface area, pore size distribution, and wettability as well as chemical composition and structure. For that purpose, such analytical techniques as scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, tensiometry, Brunauer-Emmett-Teller surface area analysis, differential scanning calorimetry, Shore D hardness test, and Charpy impact test were used. The results indicated a drop of cross-linking temperature and an extension of setting time with the addition of µHAp. The µHAp-filled acrylate composites were characterized by a globular surface morphology, higher glass transition temperature, and lower hardness and impact strength compared to nHAp-filled materials. This relationship was evident at higher nHAp concentrations.

Room temperature solvent extraction for simple and fast determination of total concentration of Ca, Cu, Fe, Mg, Mn, and Zn in bee pollen by FAAS along with assessment of the bioaccessible fraction of these elements using in vitro gastrointestinal digestion.

BACKGROUND AND AIM: Bee pollen is recognized to be a source of different nutrients, including minerals. As a food supplement, its quality and safety due to concentrations of essential macro- and microelements, and harmful trace elements has to be verified. Fast and simple element analysis of bee-collected pollen can be regarded as an important part of its quality assurance and control. The present study aimed at developping a new method for determination of selected elements (Ca, Cu, Fe, Mg, Mn, Zn) of bee pollen based on solvent extraction and completely avoiding a high temperature treatment with concentrated reagents. In addition, in vitro gastrointestinal digestion was used to assess bioavailability of elements from this food supplement. METHODS: Bee pollen samples were dried and pulverized. Total concentrations of Ca, Cu, Fe, Mg, Mn, and Zn were determined by flame atomic absorption spectrometry (FAAS) in sample solutions obtained by wet digestion (WD) in concentrated HNO3 or alternatively by solvent extraction (SE) with diluted solutions of HNO3. Gastrointestinal digestion was mimicked using simulated solutions of gastric and intestinal juices followed by determination of Ca, Cu, Fe, Mg, Mn and Zn concentrations in the bioaccessible fraction by FAAS. RESULTS: A new simple and fast method for determination of total concentrations of Ca, Cu, Fe, Mg, Mn, and Zn in bee pollen was developed and validated. The method combined room temperature, two-hour SE with 0.5 mol L-1 HNO3 with FAAS measurements versus simple standard solutions. It provided precision within 1-5 % and trueness better than 8%, and was shown to be suitable for fast analysis of different polyfloral bee pollens. In vitro gastrointestinal digestion revealed that elements were well (70-85 % for Ca, Mg) and fairly (27-43 % for Cu, Fe, Mn, and Zn) bioaccessible from bee pollen. By pouring with water and swelling overnight, bioaccessibility of studied elements from such prepared bee pollen was increased on average by less than 15 % (Mn), 20 % (Ca, Cu, Fe, Zn) or 30 % (Mn). CONCLUSIONS: Avoiding long-lasting, high-temperature wet digestion with concentrated reagents, the proposed sample treatment along with FAAS provided precise and true results of total concentrations of Ca, Cu, Fe, Mg, Mn, and Zn in bee pollen. The method was simple and fast, and enabled to analyze a higher number of samples. Simulated gastrointestinal digestion of bee pollen have shown for the first time that Ca and Mg are the most bioaccessible from this bee product. Bioaccessibility of Cu, Fe, Mg, and Zn from bee pollen are close to or lower than 40 %.

Mineral Constituents Profiling of Ready-To-Drink Nutritional Supplements by Inductively Coupled Plasma Optical Emission Spectrometry.

Nutritional drinks (NDs) are medicinal food products intended for people with different health issues constricting nutrients provision. Eight varieties of milkshake style NDs were analyzed in this work. Prior to element analysis, they were freeze-dried, and concentrations of twenty macro- and microelements in analyzed samples were simultaneously measured by ICP-OES after their mineralization in a closed-vessel microwave-assisted digestion system. Results of this analysis indicated that these NDs must be considered as nutrient-dense foods, taking into account mineral constituents. Consumption of two bottles of such NDs per day provides very a high amount or even an excess of human daily requirements set as Recommended Dietary Allowances (RDAs). Generally, concentrations of determined elements in examined NDs were consistent with data given on the labels - most of differences did not exceed 30% (median: -5.91%, standard deviation: 14%). Discovered very strong and moderate positive correlations between concentrations of major and essential elements (Ca, Mg, P, Cu, Fe, Mn, Zn) were likely due to their incorporation into formulations of analyzed NDs. However, relationships between contents of trace elements were the result of concomitance of these elements in substrates used for examined products production or contamination of substrates.

Molecular reactors for synthesis of polymeric nanocomposites with noble metal nanoparticles for catalytic decomposition of 4-nitrophenol.

HYPOTHESIS: A new, facile in-situ method for synthesis of polymeric nanocomposites (NCs) with nanoparticles (NPs) of Au, Pt and Pd is proposed. The method involves reduction-coupled sorption of Au(III), Pt(VI), and Pd(II), which avoids diffusion limitations, allowing the precipitation and stabilization of the NPs directly in the polymeric matrix. EXPERIMENTS: The obtained nanomaterials were characterized by transmission electron microscopy (TEM), and Fourier-transformation infrared spectroscopy (FT-IR). NPs loaded into polymers were also investigated using X-ray diffraction (XRD). FINDINGS: Based on the results, it was concluded that the amino functionalities simultaneously reduced noble metals ions and capped the NPs. The average diameter of the obtained AuNPs ranged from 25 to 109 nm, while reduction-coupled sorption was carried out in 1 and 3 mol L-1 HCl solutions, respectively. Applying a 0.1 mol L-1 HCl solution containing Au(III), Pd(II) and Pt(VI), a NC with AuNPs and cubic-like PdNPs was fabricated, while using a solution of the same composition, but in 3 mol L-1 HCl, resulted in formation of a NC with flower-like PtNPs. Ultimately, the selected NC based on a resin with functionalities derived from 1-(2-aminoethyl)piperazine and with bi-metallic active sites, i.e. AuNPs and PdNPs, revealed catalytic activity in the reduction of 4-nitrophenol.

Investigations of Human Fascia Lata Elemental Composition-the Effect of Different Preservation and Mineralisation Methods.

Influence of fixation medium and storage conditions as well as impact of sample mineralisation procedure on determination of minerals in human fascia lata was examined and discussed. Freezing and storage in 10% neutral buffered formalin solution and in 2.5% glutaraldehyde were used as the preservation methods of the samples. The concentrations of, both essential and toxic, elements were measured by ICP-OES method in fascia lata samples mineralised with concentrated nitric acid in a closed microwave system and in open vessels heated on a hot plate. Freezing was found as the best preserving method of fascia lata samples because of the number of elements determined and determination precision. The trace element (Cd, Cr, Cu, Fe, Ni, Sr, Zn) concentrations obtained in samples decomposed using the conventional hot plate were different from analogous measurements in solutions obtained after application of closed vessels and microwave energy assistance. Differences between the mineral compositions of fascia samples variously preserved and mineralised were statistically evaluated and discussed. Interelement correlations were analysed taking into account an impact of various methods of sample conservation. Strong, positive association between element content was discovered for Cr-Ba, Mn-Ba, P-Ba, Sr-Ba, Sr-Ca, Zn-Ca, Mn-Cr, Pb-Cr, Sr-Cr, Mg-Fe, P-Fe, Pb-Ni, Ti-Ni and Sr-P pairs of elements.

Morphological features of fascia lata in relation to fascia diseases.

Fascia lata is an important element of the fascial system, which forms the continuum of connective tissue throughout the body. This deep fascia envelops the entire thigh and hip area and its main function is to transmit mechanical forces generated by the musculoskeletal system of the lower extremities. Fascia lata is also known as a useful and easily harvested graft material. Despite its crucial role in lower extremity biomechanics and wide-ranging applications in plastic and reconstructive surgery, both the structure of fascia lata and particularly the cells populating this tissue are relatively unexplored and therefore poorly understood. The aim of this study was to characterize the main cell populations encountered within human fascia lata and to try to understand their role in health and diseases. Pathologically unchanged human fascia lata was obtained post mortem from adult males. The specimens were analyzed under light, electron, and confocal microscopy. On the basis of different visualization techniques, we were able to characterize in detail the cells populating human fascia lata. The main cells found were fibroblasts, fibrocytes, mast cells, cells showing myoid differentiation, nerve cells, and most interestingly, telocytes. Our results supplement the formerly inadequate information in the literature regarding the cellular components of deep fascial structure, may contribute to a better understanding of the pathogenesis of fascial disorders and improve fascia lata application as a graft material.

Mineral Composition and Nutritive Value of Isotonic and Energy Drinks.

Several very popular brands of isotonic and energy drinks consumed for fluid and electrolyte supplementation and stimulation of mental or physical alertness were chosen for investigation. Liquid beverages available in polyethylene bottles and aluminum cans as well as products in the form of tablets and powder in sachets were studied. The total concentrations of 21 elements (Ag, Al, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, Pb, Sr, Ti, V, and Zn), both essential and toxic, were simultaneously determined in preconcentrated drink samples by inductively coupled plasma-optical emission spectrometry (ICP-OES) equipped with pneumatic and ultrasonic nebulizers. Differences between the mineral compositions of isotonic and energy drinks were evaluated and discussed. The highest content of Na was found in both isotonic and energy drinks, whereas quite high concentrations of Mg were found in isotonic drinks, and the highest amount of calcium was quantified in energy drinks. The concentrations of B, Co, Cu, Ni, and P were higher in isotonic drinks, but energy drinks contained greater quantities of Ag, Cr, Zn, Mn, and Mo and toxic elements, as Cd and Pb. A comparison of element contents with micronutrient intake and tolerable levels was performed to evaluate contribution of the investigated beverages to the daily diet. The consumption of 250 cm(3) of an isotonic drink provides from 0.32% (for Mn) up to 14.8% (for Na) of the recommended daily intake. For the energy drinks, the maximum recommended daily intake fulfillment ranged from 0.02% (for V) to 19.4 or 19.8% (for Mg and Na).

Mineral composition of human fascia lata.

The mineral composition of pathologically unchanged human fascia lata was examined here using inductively coupled plasma optical emission spectrometry (ICP-OES) method for the first time. The total concentrations of Ag, Al, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, P, Pb, Sr, Ti, V and Zn were simultaneously measured in the tissue secured during autopsy. The age-related changes and between-gender differences in mineral composition of the examined tissue were investigated and discussed.

Metal concentrations in deciduous tree leaves from urban areas in Poland.

Accumulation of metals in deciduous tree foliage from urban areas in western-south Poland was monitored during the vegetation season of 2000 year. Concentrations of Al, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Ni, Pb, Sr, Ti and Zn were measured in birch, willow, linden and maple leaves using the ICP-AES method. Seasonal variations of metal concentrations and their relations with sampling site were investigated. The most dynamic accumulation of Al, Cd, Cr, Ni and Pb was observed for examined species. The highest differences in element concentrations for investigated sites were found for Ba, Cd, Mn and Ni. Interelement correlations were investigated. In all foliar samples synergistic relationships between Al-Cr and Ca-Sr were found. Statistically significant negative correlations were observed only for Cd and Ti in birch leaves.