ABSTRACT
Microwave (MW) therapy is an emerging therapy with high efficiency and deep penetration to combat the crisis of bacterial resistance. However, as the energy of MW is too low to induce electron transition, the mechanism of MW catalytic effect remains ambiguous. Herein, a cerium-based metal-organic framework (MOF) is fabricated and used in MW therapy. The MW-catalytic performance of CeTCPP is largely dependent on the ions in the liquid environment, and the electron transition is achieved through a "tribovoltaic effect" between water molecules and CeTCPP. By this way, CeTCPP can generate reactive oxygen species (ROS) in saline under pulsed MW irradiation, showing 99.9995 ± 0.0002% antibacterial ratio against Staphylococcus aureus (S. aureus) upon two cycles of MW irradiation. Bacterial metabolomics further demonstrates that the diffusion of ROS into bacteria led to the bacterial metabolic disorders. The bacteria are finally killed due to "amino acid starvation". In order to improve the applicability of CeTCPP, It is incorporated into alginate-based hydrogel, which maintains good MW catalytic antibacterial efficiency and also good biocompatibility. Therefore, this work provides a comprehensive instruction of using CeTCPP in MW therapy, from mechanism to application. This work also provides new perspectives for the design of antibacterial composite hydrogel.
ABSTRACT
Currently, titanium and its alloys have emerged as the predominant metallic biomaterials for orthopedic implants. Nonetheless, the relatively high post-operative infection rate (2-5%) exacerbates patient discomfort and imposes significant economic costs on society. Hence, urgent measures are needed to enhance the antibacterial properties of titanium and titanium alloy implants. The titanium dioxide nanotube array (TNTA) is gaining increasing attention due to its topographical and photocatalytic antibacterial properties. Moreover, the pores within TNTA serve as excellent carriers for chemical ion doping and drug loading. The fabrication of TNTA on the surface of titanium and its alloys can be achieved through various methods. Studies have demonstrated that the electrochemical anodization method offers numerous significant advantages, such as simplicity, cost-effectiveness, and controllability. This review presents the development process of the electrochemical anodization method and its applications in synthesizing TNTA. Additionally, this article systematically discusses topographical, chemical, drug delivery, and combined antibacterial strategies. It is widely acknowledged that implants should possess a range of favorable biological characteristics. Clearly, addressing multiple needs with a single antibacterial strategy is challenging. Hence, this review proposes systematic research into combined antibacterial strategies to further mitigate post-operative infection risks and enhance implant success rates in the future.
ABSTRACT
Large doses and long duration are often required for herbal medicines to kill bacteria effectively. Herein, a photoacoustic interfacial engineering strategy was utilized to endow curcumin (Cur, a kind of herbal medicine) with rapid and highly effective bacteria-killing efficacy, in which Cur was combined with CuS to form a hybrid material of CuS/Cur with tight contact through in situ nucleation and growth on the petaloid CuS surface. Due to the different work functions of CuS and Cur, the interfacial electrons were redistributed, i.e., a large number of electrons gathered on the side of CuS. In contrast, the holes gathered on the side of Cur after contact. An internal electric field was formed to drive the excited electrons to transfer from CuS to Cur, thus enhancing the separation of electron-hole pairs. Besides exerting the drug nature of Cur itself, the CuS/Cur hybrid also had photo-sono responsive ability, which endowed the hybrid with photothermal, photodynamic, and sonodynamic effects. Therefore, this Cur-based hybrid killed 99.56% of Staphylococcus aureus and 99.48% of Escherichia coli under 808 nm near-infrared light irradiation and ultrasound successively for 15 min, which was ascribed to the synergy of ROS, hyperthermia, and released Cu2+ together with the drug properties of Cur. This work provides a strategy to enhance the therapeutic effects of herbal medicines against pathogenic bacterial infections by exciting the intrinsic properties of herbal medicines as materials through a photo-sono interfacial engineering strategy.
Subject(s)
Nanomedicine , Nanoparticles , Copper/pharmacology , Phototherapy , Staphylococcus aureus , Escherichia coli , Nanoparticles/therapeutic useABSTRACT
Knowledge on the impacts of microplastics (MPs) pollution on freshwater environments and biota remains limited. Meanwhile, freshwater ecosystems have been threatened by elevated temperatures caused by climate change. To date, no information exists on how MPs-especially under elevated temperature conditions-affect predatory performance, digestive processes and metabolic pathways in freshwater organisms. Here, we examined MPs, elevated temperature and their combined effects on juveniles (0+ group) of an Amazonian cichlid, the discus fish (Symphysodon aequifasciatus). For 30â¯days, fish were exposed to ambient or elevated temperatures (i.e., 28 or 31⯰C) in the absence or presence of MPs (i.e., 0 or 200⯵g/L). The following metrics were quantified: MPs accumulation; predatory performance; and biomarkers involved in neurotransmission, digestion and energy production. The results showed that survival rate and body length were not affected by MPs, elevated temperatures or their combination. Elevated temperatures resulted in an increase in MP concentrations in fish bodies. Exposure to MPs decreased the post-exposure predatory performance (PEPP) at ambient temperatures but not at elevated temperatures. Elevated temperatures, however, had no effect on the PEPP but antagonistically interacted with MPs, leading to similar predatory performances under present and future conditions. Acetylcholinesterase (AChE) activity was only affected by MPs and decreased in the presence of MPs, indicating adverse effects in nervous and neuromuscular function and, thus, potentially in predatory performance. Trypsin activity was only influenced by MPs and decreased during exposure to MPs. Elevated temperatures or MPs alone increased the amylase activity but interacted antagonistically. Lipase activity was not influenced by either of the two stressors. In contrast, alkaline phosphatase (ALP) activity was affected by MPs or elevated temperatures alone and decreased with both stressors. Such results indicate deficits in the digestive capabilities of early-stage S. aequifasciatus under elevated temperature conditions and especially during exposure to MPs. Electron transport system (ETS) activity was not influenced by either of the two stressors. Both elevated temperatures and MPs alone increased LDH activity; however, the interaction between the two stressors cancelled activity but was still higher than activity in present conditions. Citrate synthase (CS) activity decreased with elevated temperature but increased during exposure to MPs. Cytochrome c oxidase (COX) activity was only influenced by MPs and increased in the presence of MPs. Thus, S. aequifasciatus juveniles exposed to elevated temperatures and MPs not only relied on anaerobic glycolysis for energy production but also depended on aerobic metabolism in the presence of MPs. Overall, these findings suggested that MPs showed a greater impact than elevated temperatures on the predatory performance, digestion and energy production of S. aequifasciatus. Nevertheless, juvenile survival and growth were minimally impacted, and thus, S. aequifasciatus could cope with near-future temperature increases and MP exposure.
Subject(s)
Cichlids/physiology , Digestion/drug effects , Energy Metabolism/drug effects , Plastics/toxicity , Predatory Behavior/drug effects , Temperature , Acetylcholinesterase/metabolism , Analysis of Variance , Animals , Cichlids/growth & development , Climate Change , Environmental Monitoring , Enzymes/metabolism , Water Pollutants, Chemical/toxicityABSTRACT
A statistics of sky survey for interstellar H2O and OH masers is presented. These masers can be classified into 3 categories. The total number of sources in each category is of the same order of magnitude, and they can maintain--10(5) years. But there are fewer than half masers with detectable H II regions. An improved radiative pumping mechanism is proposed, and it overcame the known drawback of former radiative models. The observationally statistical results can be interpreted by both the new mechanism and the evolutional model with gravitational force of star responsible for the H II region.