Self-assembly and dynamic exchange of cuboctahedral metal-organic cages.

Due to their unique physical and chemical properties, metal-organic cage structures have great potential for applications in various fields. However, current studies have mainly focused on highly symmetric structures assembled from single metal ions and organic ligands, limiting their diversity and complexity, and there are still relatively few studies on the dynamic formation process of metal-organic cages. Herein, we constructed a series of metal-organic cages with different sizes assembled from the highly-stable coordination of 2,2':6',2''-terpyridine-based tetratopic ligands and various metals ions such as Zn, Cu, Co and Fe. Furthermore, the intermolecular exchange process between the metal-organic cages was explored through the dynamic exchange of ligands, and the formation of a series of hybrid supramolecular nanocages together with their final tendency to form a predominant structure of M24L14L28 was observed. In addition, the binding of metal-organic cages with 5,10,15,20-tetrakis(3,4,5-trimethoxyphenyl) porphyrin-Zn was also investigated. This study not only expands the complexity and diversity of metal-organic cages, but also provides a new perspective for studying the dynamic behaviour of metal-organic cages.

Newborn concurrent hearing and genetic screening for hearing impairment: A systematic review and meta­analysis.

Hearing loss is the most prevalent neurosensory disorder in humans, with significant implications for language, social and cognitive development if not diagnosed and treated early. The present systematic review and meta-analysis aimed to determine the rate of hearing screening pass and genetic screening failure [universal newborn hearing screening (UNHS) pass/genetic failure] and to investigate the advantages of combining newborn hearing and genetic screening for newborn hearing impairment. The PubMed, Embase and Cochrane databases were searched from inception to September 2023 to identify studies reporting the combination of neonatal hearing screening with genetic screening. Duplicate literature, unpublished literature, studies with incomplete data, animal experiments, literature reviews and systematic studies were excluded. All the data were processed by STATA15.1 statistical software. A total of nine cross-sectional studies were included in this meta-analysis. The sample sizes ranged from 1,716 to 180,469, and there were a total of 377,688 participants. The pooled results revealed that the prevalence of passing the UNHS while failing genetic screening was 0.31% (95% CI, 0.22-0.41%). The prevalence of UNHS pass and gap junction protein beta 2 and solute carrier family 26 member 4 variant screen failure was 0.01% (95% CI, 0.00-0.02%) and 0.00% (95% CI, 0.00%), respectively, while the prevalence of mitochondrially encoded 12S RRNA variant screening failure and UNHS pass was 0.21% (95% CI, 0.18-0.26%). Combined screening has a significant advantage over pure hearing screening, especially in terms of identifying newborns with mitochondrial gene mutations that render them sensitive to certain medications. In clinical practice, decision-makers can consider practical circumstances and leverage the benefits of combined newborn hearing and genetic screening for early diagnosis, early counseling, and early intervention in patients with hearing loss.

Flexible/Regenerative Nanosensor with Automatic Sweat Collection for Cytokine Storm Biomarker Detection.

The real-time monitoring of low-concentration cytokines such as TNF-α in sweat can aid clinical physicians in assessing the severity of inflammation. The challenges associated with the collection and the presence of impurities can significantly impede the detection of proteins in sweat. This issue is addressed by incorporating a nanosphere array designed for automatic sweat transportation, coupled with a reusable sensor that employs a Nafion/aptamer-modified MoS2 field-effect transistor. The nanosphere array with stepwise wettability enables automatic collection of sweat and blocks impurities from contaminating the detection zone. This device enables direct detection of TNF-α proteins in undiluted sweat, within a detection range of 10 fM to 1 nM. The use of an ultrathin, ultraflexible substrate ensures stable electrical performance, even after up to 30 extreme deformations. The findings indicate that in clinical scenarios, this device could potentially provide real-time evaluation and management of patients' immune status via sweat testing.

Chitosan/polyvinyl alcohol food packaging incorporated with purple potato anthocyanins and nano-ZnO: Application on the preservation of hairtail (Trichiurus haumela) during chilled storage.

The objective of this work was to evaluate the potential application of chitosan/PVA food packaging films incorporating nano-ZnO and purple potato anthocyanins for preserving chilled hairtail pieces. The hairtail pieces were packaged with chitosan/PVA (CP) and chitosan/PVA/nano-ZnO/purple potato anthocyanins (CPZP), respectively, and Control named without any packaging. The changes in pH, total volatile basic nitrogen (TVB-N), total bacterial colony (TVC), thiobarbituric acid (TBA), color value, and sensory evaluation scores of hairtail pieces were periodically determined. Notably, pH, TVC, TVB-N and TBA values of CPZP group on day 15 were 11.67 %, 23.71 %, 80.73 %, and 35.07 %, respectively, lower than Control group. In addition, CPZP group also performed the best in color and sensory evaluation. These results indicated that CPZP, an active food packaging, could extend the shelf-life of hairtail at least 6 days. Overall, chitosan/PVA food films incorporated with nano-ZnO and purple potato anthocyanins (180 mg/100 mL) provides a potential application in food preservation.

A two-stage maintenance trial of cetuximab-based treatment in RAS and BRAF wild-type unresectable metastatic colorectal cancer: a retrospective real-world study.

Background: To investigate the effectiveness and safety of maintenance regimens based on cetuximab, we conducted a real-world, single-arm, retrospective study at a single center. Methods: In Fujian Medical University Union Hospital, patients with unresectable metastatic colorectal cancer (mCRC) who received cetuximab-based maintenance therapy between December 2020 and December 2021 were included. All patients had RAS and BRAF wild-type. The maintenance regimen consisted of 6-12 cycles of cetuximab plus irinotecan (Phase 1) and cetuximab (Phase 2). Patients could receive reintroduction therapy in case of disease progression during Phase 2. Progression-free survival (PFS), overall survival (OS), and safety data were collected. Results: According to the inclusion and exclusion criteria of the study, a total of 108 subjects who received maintenance therapy were included- 51 experienced disease progression during Phase 1, with PFS (1) of 7.3 months. Among the 52 patients who entered Phase 2, 17 were still in this phase at the end of follow-up, with PFS (2) of 10.1 months. In Phase 2, 35 patients experienced disease progression, of whom 24 received reintroduction therapy, with PFS (3) of 6.7 months. The overall PFS (total) during the maintenance period was 11.9 months, and the OS was 39.2 months. Grade III or higher adverse events were 4.6% during Phase 1 and 0% during Phase 2. Conclusion: Innovative cetuximab-based maintenance therapy showed a trend toward improving the prognosis of mCRC patients with RAS and BRAF wild-type, while the toxic side effects of maintenance therapy were manageable. Clinical trial registration: https://www.chictr.org.cn, identifier ChiCTR2000040940.

Effect of lidocaine pumped through hepatic artery to relieve pain of hepatic artery infusion chemotherapy.

Background: This study aims to explore the analgesic effect of lidocaine administered through the hepatic artery during hepatic artery infusion chemotherapy (HAIC) for hepatocellular carcinoma (HCC). Methods: A total of 45 HCC patients were randomly divided into a study group and a control group. Both groups received oxaliplatin (OXA) based FOLFOX protocol via electronic infusion pump. The study group was continuously infused with 100 mg of lidocaine during HAIC, while 5% glucose solution was infused in the same way as described above. Changes in vital signs, visual analogue score (VAS) and general comfort score (GCQ scale) were recorded before surgery (Time point 0), at the end of infusion (Time point 01), 1 h after HAIC (Time point 02), 3 h after HAIC (Time point 03) and 6 h after HAIC (Time point 04). Results: At each point of time from Time point 0 through Time point 04, the differences in MAP, RR and SPO2 between the two groups were not statistically significant (P > 0.05). At each point of time from Time point 01 through Time point 04, the mean VAS scores in the study group were smaller and GCQ scores were higher than those in the control group, and the differences were both statistically significant (P < 0.05). Conclusions: Lidocaine infusion through the hepatic artery during HAIC effectively reduces intraoperative and postoperative pain and improves patient satisfaction with pain management, making it a valuable technique for clinical practice.

A bibliometric analysis of radiation-induced brain injury: a research of the literature from 1998 to 2023.

BACKGROUND: Radiation-induced brain injury (RIBI) is a debilitating sequela after cranial radiotherapy. Research on the topic of RIBI has gradually entered the public eye, with more innovations and applications of evidence-based research and biological mechanism research in the field of that. This was the first bibliometric analysis on RIBI, assessing brain injury related to radiation articles that were published during 1998-2023, to provide an emerging theoretical basis for the future development of RIBI. METHODS: Literature were obtained from the Web of Science Core Collection (WOSCC) from its inception to December 31, 2023. The column of publications, author details, affiliated institutions and countries, publication year, and keywords were also recorded. RESULTS: A total of 2543 journal articles were selected. The annual publications on RIBI fluctuated within a certain range. Journal of Neuro-oncology was the most published journal and Radiation Oncology was the most impactful one. LIMOLI CL was the most prolific author with 37 articles and shared the highest h-index with BARNETT GH. The top one country and institutions were the USA and the University of California System, respectively. Clusters analysis of co-keywords demonstrated that the temporal research trends in this field primarily focused on imaging examination and therapy for RIBI. CONCLUSION: This study collects, visualizes, and analyzes the literature within the field of RIBI over the last 25 years to map the development process, research frontiers and hotspots, and cutting-edge directions in clinical practice and mechanisms related to RIBI.

Applications of Artificial Intelligence in Psychiatric Nursing: A Scope Review.

Rapid advances in artificial intelligence (AI) have reshaped healthcare, including psychiatric nursing, to address the limitations of traditional approaches and meet escalating mental health challenges. A scoping review analyzed 48 articles examining the application of AI in psychiatric nursing across different technologies and topics, noting trends in publications and countries involved. The articles covered different aspects of mental health using AI technologies such as machine learning and robotics, and primarily explored AI applications in mental health, specifically dementia, autism and schizophrenia. These studies highlighted the role of AI in personalized care plans, symptom monitoring and risk assessment. AI is promising, but faces challenges such as data bias and ethical concerns. Future research needs to focus on long-term studies, diverse populations, patient interaction and personalized treatments for practical integration into psychiatric nursing.

Multidisciplinary-Based Design of a Nursing Informatics Curriculum.

This paper explores the development of a multidisciplinary nursing informatics curriculum. The aim is to integrate multiple disciplines to meet the educational objectives of undergraduate nursing students. The curriculum design included a thorough needs assessment, curriculum development, and evaluation. Of the 29 undergraduate nursing students enrolled in the course, 21 completed the course evaluation with a response rate of 72.4%. The comprehensive assessment of the course (part 1) scored 93.7 out of 100, while the quality of the teacher's teaching (part 2) scored 93.4 out of 100. In addition, in response to the subjective questions, students expressed satisfaction with the course design and teaching methods, and emphasized the usefulness of the course for their future nursing practice.

Understanding Nursing Informatics: A Survey of Nurses' Perception.

This paper aims to explore nurses' understanding of nursing informatics (NI). The structured and open-ended questionnaires were used to explore their knowledge of NI. Between 17 and 18 October 2023, the survey was conducted via a web portal and targeted 124 nurses attending in a nursing management training programme at the West China Hospital, Sichuan University. A total of 57.3% (71/124) of the nurses completed the survey. Of these, 29.6% (21/71) were unaware of NI and 70.4% (50/71) were aware of NI. However, only one of the nurses gave an accurate definition of NI. The results of this study suggest that there is a limited understanding of NI among senior nurses in mainland China, indicating a need to improve NI education for nurses.

Nursing Undergraduates' Perceptions of Nursing Informatics Courses.

The aim of this paper is to explore how nursing undergraduates perceive a nursing informatics (NI) course. Between 7 and 14 September 2023, a survey was conducted with 51 nursing undergraduates who enrolled in 2021. A structured and open-ended questionnaire was used to explore their perceptions of the course. A total of 90.2% (46/51) of the students completed the survey. Of the respondents, 69.6% (32/46) were unaware of NI, while 30.4% (14/46) were aware of it. Furthermore, 93.5% (43/46) of the respondents supported the introduction of a nursing informatics course. The results of this study will guide the development of NI curricula.

Evaluation of a Nursing Informatics Course by Nurses: A Continuing Education.

This study aimed to evaluate the effectiveness of a nursing informatics continuing education course and nurses' perceptions of it. This study investigates the evaluation and satisfaction of 103 nurses who attended the course on 10-11 June 2023. The survey was divided into two parts: the first part focused on the evaluation of teaching and the second part focused on the evaluation of the course. The first part contained 7 structured questions and 1 open-ended question, while the second part contained 11 structured questions. The results show a high level of satisfaction, with the teaching receiving a score of 9.9 out of 10. 95% of the participants were "very satisfied" and 5% were "satisfied".

Perspectives of Multidisciplinary Faculty on the Design of Nursing Informatics Curriculum.

This study aimed to understand the experiences and challenges of interdisciplinary collaboration in nursing informatics curriculum design. The study was conducted from 9-20 August 2023 with 14 multidisciplinary faculty members. Surveys and interviews focused on perceptions of interdisciplinary communication, importance of collaboration, challenges encountered, and training needs to identify barriers to effective curriculum development. Participation was voluntary and anonymous. The results revealed significant challenges, including differences in terminology and thought processes between disciplines, role ambiguity, and cultural/methodological differences. The results of the study highlight the importance of collaboration, which is critical to the development of an effective and cohesive nursing informatics curriculum.

Perception of ChatGPT by Nursing Undergraduates.

The study aimed to investigate the awareness and use of ChatGPT among undergraduate nursing students. A structured questionnaire was used to assess the awareness and use of ChatGPT. The questionnaire was undergraduate nursing students enrolled in 2021. The response rate to the survey was 90.2% (46/51). Of the respondents, 45 students were aware of ChatGPT, and only one student was not aware of ChatGPT. In terms of usage, 23 students responded. Among them, 16 used ChatGPT to enhance their learning experience, six for homework, five for chatting, four for essay writing, and one for other purposes. This study provides valuable insights for the better use of ChatGPT in nursing education.

Nurses' Perspectives on ChatGPT: A Survey Study.

The aim of this study was to assess nurses' awareness and use of ChatGPT. The study was conducted in October 2023 with an online questionnaire for 124 nurses in the nursing education programme at West China Hospital. The questionnaire included participants' demographic information, awareness of ChatGPT, and actual experience of using it. A total of 57.3% (71/124) of the nurses completed the survey. Of these, 56.3% (40/71) were aware of ChatGPT and 43.7% (31/71) were not aware of ChatGPT. In terms of use, of the 20 who used ChatGPT, 13 used it for studying, 10 for essay writing, five for research and two for chatting. This study highlights the potential of ChatGPT to improve nurses' professional competence and effectiveness. Further research will focus on how ChatGPT can be used more effectively to support nurses' professional development and growth.

Evaluation of Acupuncture Efficacy in Modulating Brain Activity With Periodic-Aperiodic EEG Measurements.

Acupuncture is an important therapeutic method of traditional Chinese medicine and can effectively modulate brain disorders. The therapeutic efficacy of acupuncture is hard to evaluate due to lacking of effective measurements of brain activity. In this work, we design an EEG-based monitoring system to evaluate therapeutic effect of acupuncture on human brain by extracting periodic-aperiodic features. Power spectral density is estimated to compute the adjusted power of periodic oscillatory rhythm in EEG under acupuncture stimulation. It is exhibited that the brain activity in alpha band (8-12 Hz) is significantly enhanced during acupuncture, especially in parietal and occipital lobe regions. To probe the modulatory effect of acupuncture on aperiodic brain activity, we calculate the aperiodic exponent based on the parameterization of EEG power spectra. The aperiodic exponent decreases along with acupuncture process, which is more significant in central and frontal lobe regions. Furthermore, sensitivity of different brain regions to acupuncture is assessed by the integration of adjusted power and aperiodic exponent. Experimental results demonstrate the effectiveness of proposed periodic-aperiodic measurements of EEG signals, by which different effects of four acupuncture manipulations are precisely evaluated and a knowledge graph is established. The monitoring system provides a new perspective to quantitatively evaluate acupuncture effect on human brain and improve its therapeutic efficacy in clinical applications for neural disorders.

Research progresses on mitochondrial-targeted biomaterials for bone defect repair.

In recent years, the regulation of the cell microenvironment has opened up new avenues for bone defect repair. Researchers have developed novel biomaterials to influence the behavior of osteoblasts and immune cells by regulating the microenvironment, aiming to achieve efficient bone repair. Mitochondria, as crucial organelles involved in energy conversion, biosynthesis and signal transduction, play a vital role in maintaining bone integrity. Dysfunction of mitochondria can have detrimental effects on the transformation of the immune microenvironment and the differentiation of stem cells, thereby hindering bone tissue regeneration. Consequently, targeted therapy strategies focusing on mitochondria have emerged. This approach offers a wide range of applications and reliable therapeutic effects, thereby providing a new treatment option for complex and refractory bone defect diseases. In recent studies, more biomaterials have been used to restore mitochondrial function and promote positive cell differentiation. The main directions are mitochondrial energy metabolism, mitochondrial biogenesis and mitochondrial quality control. In this review, we investigated the biomaterials used for mitochondria-targeted treatment of bone defect repair in recent years from the perspective of progress and strategies. We also summarized the micro-molecular mechanisms affected by them. Through discussions on energy metabolism, oxidative stress regulation and autophagy regulation, we emphasized the opportunities and challenges faced by mitochondria-targeted biomaterials, providing vital clues for developing a new generation of bone repair materials.

Exploring the temporal dynamics of a disease suppressive rhizo-microbiome in eggplants.

The rhizosphere microbiome is important for plant health, yet their contributions to disease resistance and assembly dynamics remain unclear. This study employed rhizosphere microbiome transplantation (RMT) to delineate the impact of the rhizosphere microbiome and the immune response of eggplant (Solanum melongena) on resistance to bacterial wilt caused by Ralstonia solanacearum. We first identified disease-suppressive and disease-conducive rhizosphere microbiome in a susceptible tomato recipient. Using a non-destructive rhizobox and 16S rRNA amplicon sequencing, we monitored the dynamics of both microbiome types during the eggplant development. Most differences were observed at the early stage and then diminished over time. The suppressive microbiome maintained a higher proportion of initial community members throughout eggplant development and exhibited stronger deterministic processes in the early stage, underscoring the importance of plant selection in recruiting protective microbes for rhizosphere immunity. Our study sheds light on the development of microbiome-based strategies for plant disease management and resistance breeding.

A novel bioaccessibility-based probabilistic risks assessment of potentially toxic elements (PTEs) in earthworm.

Introduction: Early risk assessment studies usually based on total heavy metal (loid) contents, inevitably leading to an overestimation of the health risks. In addition, inputs are represented as single-point estimates in deterministic models, leading to underestimation or overestimation of the health risks. Methods: To overcome these barriers, a novel probabilistic risk assessment strategy based on the combinational use of bioaccessibility and Monte Carlo simulation was developed to assess heavy metal (loid) associated health risks of earthworms in this study. To obtain a realistic and robust probabilistic risk assessment, heavy metal (loid) exposure duration and frequency were determined using our questionnaire data. Results: As a result, the mean gastrointestinal bioaccessibility was in the order: Cd > As > Cu > Hg. The mean hazard index (HI) values for investigated metal (loid)s were 0.65 and 0.59 for male and female, respectively, demonstrating an acceptable health risk in an average community. However, the 90th percentile of HI values was 1.87 and 1.65 for male and female, respectively. And the total non-cancer risks of heavy metal (loid) exposure exceeded the acceptable threshold for 19.9% and 17.8% of male and female, respectively. In addition, the total cancer risk (TCR) value through co-exposure to As and Cd suggested that the carcinogenic risks may be of concern for average exposure population. Sensitivity analyses revealed that the exposure frequency and bioaccessible As concentration were the dominant contributors to the total risk variance, which provided meaningful implications for environmental management. Conclusion: Altogether, the refined strategy based on bioaccessibility and Monte Carlo simulation is the first of its kind, such effort attempts to scientifically guide the rational clinic use of TCM and the improvement of population-health.

PhieDBEs: a DBD-containing, PAM-flexible, high-efficiency dual base editor toolbox with wide targeting scope for use in plants.

Dual base editors (DBEs) enable simultaneous A-to-G and C-to-T conversions, expanding mutation types. However, low editing efficiency and narrow targeting range limit the widespread use of DBEs in plants. The single-strand DNA binding domain of RAD51 DBD can be fused to base editors to improve their editing efficiency. However, it remains unclear how the DBD affects dual base editing performance in plants. In this study, we generated a series of novel plant DBE-SpGn tools consisting of nine constructs using the high-activity cytidine deaminase evoFERNY, adenosine deaminase TadA8e and DBD in various fusion modes with the PAM-flexible Streptococcus pyogenes Cas9 (SpCas9) nickase variant SpGn (with NG-PAM). By analysing their editing performance on 48 targets in rice, we found that DBE-SpGn constructs containing a single DBD and deaminases located at the N-terminus of SpGn exhibited the highest editing efficiencies. Meanwhile, constructs with deaminases located at the C-terminus and/or multiple DBDs failed to function normally and exhibited inhibited editing activity. We identified three particularly high-efficiency dual base editors (C-A-SpGn, C-A-D-SpGn and A-C-D-SpGn), named PhieDBEs (Plant high-efficiency dual base editors), capable of producing efficient dual base conversions within a narrow editing window (M5 ~ M9, M = A/C). The editing efficiency of C-A-D-SpGn was as high as 95.2% at certain target sites, with frequencies of simultaneous C-to-T and A-to-G conversions as high as 81.0%. In summary, PhieDBEs (especially C-A-D-SpGn) can produce diverse mutants and may prove useful in a wide variety of applications, including plant functional genomics, precise mutagenesis, directed evolution and crop genetic improvement, among others.