ABSTRACT
BACKGROUND: The worldwide seroprevalence of human BK polyomavirus (BKV) in adults is 80%. About 10%-60% of renal transplant recipients experience BKV infection, nephropathy of the graft may occur in 5% of the cases, and up to 45% lose the graft. The aim of this work was to describe the prevalence of BK viruria during the 1st year after transplantation. METHODS: An epidemiologic multicenter cross-sectional study was carried out in consecutive patients at each site with kidney transplantation from August 2011 to July 2012. Clinically significant viruria was defined as >10(7) copies/mL. Viral DNA was extracted with the use of silica columns. Quantification was performed with the use of real-time polymerase chain reaction with primers that amplify a fragment of the large T-antigen gene and with a specific Taqman-MGB probe for BKV. For each assay, a standard curve with a quantified plasmid was included. RESULTS: Of 402 renal transplant recipients at 18 renal transplant sites, we analyzed 382; median age was 46.33 years, and 46.40% were female. The median of the temporal distribution for urine samples was 153 days. BK virus was detected in 50/382 samples (13%), 18 with values >10(7) copies/mL (4.7%). The median of the distribution of positive values was 123 days and the highest frequency of positive values was in months 3-7. The conditions of recipient older than 34 years and donor older than 41 years were the only ones that showed statistically significant association with BK viruria. No association with any specific immunosuppressive drug was observed. CONCLUSIONS: This is the first multicenter study conducted in Argentina to determine the prevalence of BK viruria in renal transplant recipients. Because of the growing number of the population susceptible to this infection, it is important to register and describe data about its epidemiology and associated risk factors.
Subject(s)
BK Virus/isolation & purification , Kidney Transplantation , Opportunistic Infections/epidemiology , Polyomavirus Infections/epidemiology , Postoperative Complications/epidemiology , Tumor Virus Infections/epidemiology , Adult , Argentina , BK Virus/genetics , Cross-Sectional Studies , DNA, Viral/analysis , Female , Humans , Immunosuppressive Agents/adverse effects , Male , Middle Aged , Opportunistic Infections/diagnosis , Opportunistic Infections/etiology , Polyomavirus Infections/diagnosis , Polyomavirus Infections/etiology , Postoperative Complications/diagnosis , Prevalence , Real-Time Polymerase Chain Reaction , Risk Factors , Tumor Virus Infections/diagnosis , Tumor Virus Infections/etiologyABSTRACT
Numerosos autores han informado sobre la influencia del polimorfismo del complejo principal de histocompatibilidad en una correcta o defectuosa presentación antígena, y su relación con la posible susceptibilidad o resistencia a desarrollar enfermedades neoplásicas. Objetivos: 1) Determinación de variantes alélicas del HLA clase I, y su posible vinculación a la susceptibilidad o resistencia en el desarrollo de tumores mamarios y de cérvix uterino. 2) Comparar nuestros resultados con los reportados por otros autores. Material y método: evaluamos 78 pacientes (ptes.), 46 con cáncer de mama, 32 con cáncer de cérvix y 94 testigos, los cuales fueron tipificados para los locus A y B del HLA clase I, utilizando amplificación genérica por PCR, y analizados por hibridación con sondas de oligonucleótidos alelo específica...
Subject(s)
Humans , Female , Breast Neoplasms , Disease Susceptibility , Genetic Predisposition to Disease , Histocompatibility Antigens , Uterine Cervical Neoplasms , Alleles , Histocompatibility Antigens Class I , Major Histocompatibility Complex , Risk FactorsABSTRACT
Numerosos autores han informado sobre la influencia del polimorfismo del complejo principal de histocompatibilidad en una correcta o defectuosa presentación antígena, y su relación con la posible susceptibilidad o resistencia a desarrollar enfermedades neoplásicas. Objetivos: 1) Determinación de variantes alélicas del HLA clase I, y su posible vinculación a la susceptibilidad o resistencia en el desarrollo de tumores mamarios y de cérvix uterino. 2) Comparar nuestros resultados con los reportados por otros autores. Material y método: evaluamos 78 pacientes (ptes.), 46 con cáncer de mama, 32 con cáncer de cérvix y 94 testigos, los cuales fueron tipificados para los locus A y B del HLA clase I, utilizando amplificación genérica por PCR, y analizados por hibridación con sondas de oligonucleótidos alelo específica...(AU)
Subject(s)
Humans , Female , Breast Neoplasms/genetics , Uterine Cervical Neoplasms/genetics , Disease Susceptibility , Histocompatibility Antigens , Genetic Predisposition to Disease , Alleles , Risk Factors , Histocompatibility Antigens Class I , Major Histocompatibility ComplexABSTRACT
Host genetic factors, such as HLA alleles, are important in human immunodeficiency virus (HIV) infection and its progression to AIDS. HLA class I gene products are involved in peptide presentation, and each allele is responsible for presenting a different set of peptides to cytotoxic T lymphocytes. The increase or decrease in the frequency of certain alleles in HIV-1-positive versus control subjects would suggest that alleles play a role in susceptibility to or protection against this viral infection. In the present study, 56 HIV-1-positive patients in Chaco Province, Argentina, were typed for the HLA-A and HLA-B loci, using polymerase chain reaction and sequence-specific oligonucleotide probes. The frequency of alleles A*24, B*18, and B*39 was increased in HIV-1-positive subjects, suggesting that these alleles play a role in susceptibility to HIV-1 infection. Alleles B*44 and B*55 were not found in HIV-1-positive subjects, suggesting that they have a protective effect against the disease. The P values of the alleles B*39 and B*44 were statistically significant (P<.05).