ABSTRACT
Biochemical and molecular analysis was used for identification of different kefir yeasts species from Brazil, Canada and the United States of America. The sugar/ethanol-resistant activity of the yeasts was evaluated. Saccharomyces cerevisiae and Kluyveromyces marxianus had the highest growth rates, suggesting biotechnological applications possible for these strains.
ABSTRACT
Biochemical and molecular analysis was used for identification of different kefir yeasts species from Brazil, Canada and the United States of America. The sugar/ethanol-resistant activity of the yeasts was evaluated. Saccharomyces cerevisiae and Kluyveromyces marxianus had the highest growth rates, suggesting biotechnological applications possible for these strains.
Subject(s)
Base Sequence , Cultured Milk Products , Ethanol/analysis , Genome, Bacterial , In Vitro Techniques , Yeasts/genetics , Yeasts/isolation & purification , Phenotype , Polymerase Chain Reaction/methods , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/isolation & purification , Electrophoresis , Genetic Variation , Genotype , MethodsABSTRACT
Biochemical and molecular analysis was used for identification of different kefir yeasts species from Brazil, Canada and the United States of America. The sugar/ethanol-resistant activity of the yeasts was evaluated. Saccharomyces cerevisiae and Kluyveromyces marxianus had the highest growth rates, suggesting biotechnological applications possible for these strains.(AU)
Subject(s)
Biochemistry , Ethanol/analysis , Sugars , Biotechnology/trends , Yeasts/ultrastructure , Saccharomyces cerevisiaeABSTRACT
The ovarian cystoadenofibroma are tumors not very frequently of superficial epithelium. It can appears at all ages. It can form fibrotic nodules of strong consistency that initially appear with the papillae and it forms nodule when the time goes by; generally, they are unilateral. We studied 10 cases of cystoadenofibromas in a period of 5 years, the patients' age was from 18 to 68 years old being more frequent in the adult age; seven cases were unilateral, three were bilateral, two of these appeared clinically as acute abdomen due to rupture of the tumor the third one was a hysterectomy is finding. In seven cases there was association with uterine leiomiomatosis mainly in older patients. Macroscopically it was seen that in younger patients these tumors were cystic with small papillae, and in older patients they formed fibrotic nodules. The immunohystochemical study with Vimentin and Desmin showed that the Desmin was focally immunopositive in form of fine fibers at the union with superficial epithelium and greatly positive fibrous portion for Vimentina.