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1.
Front Vet Sci ; 11: 1322871, 2024.
Article in English | MEDLINE | ID: mdl-38988978

ABSTRACT

Epidural anesthesia in dogs is a locoregional anesthesia technique used in veterinary medicine, becoming an important integrated application in the anesthetic protocol to provide safer and more effective analgesia to patients. For this, professionals must adhere to rigorous guidelines and possess technical skills. In this context, in veterinary education, the development of practical clinical skills represents a crucial aspect in the training of these professionals. However, traditional teaching methods have proven insufficient to ensure a consistent level of competence among recent graduates. The introduction of non-animal alternatives for educational purposes has contributed to the development of simulation-based teaching, an innovative and accessible field capable of enhancing pre-clinical proficiency in students and reducing the use of live animals and cadavers. Despite its application in various areas of veterinary education, there are no conclusive results regarding the development of accessible simulators capable of effectively enhancing training in epidural anesthesia in dogs. Therefore, this article represents a pioneering study aimed at sharing a method for creating SimuVet, a realistic simulator for training epidural anesthesia in dogs. The simulator was fully developed by veterinary researchers with limited experience in 3D printing and, after preliminary analysis, demonstrated excellent performance and ultrasonographic anatomy. Future work will focus on the formal validation of this simulator with the aim of improving the teaching and learning process for students and experts in performing epidural anesthesia in companion animals.

2.
Microorganisms ; 7(11)2019 Nov 05.
Article in English | MEDLINE | ID: mdl-31694172

ABSTRACT

The genus Ehrlichia is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. Ehrlichia includes important pathogens affecting canids (E. canis, E. chaffeensis, and E. ewingii), rodents (E. muris), and ruminants (E. ruminantium). Ehrlichia minasensis, an Ehrlichia closely related to E. canis, was initially reported in Canada and Brazil. This bacterium has now been reported in Pakistan, Malaysia, China, Ethiopia, South Africa, and the Mediterranean island of Corsica, suggesting that E. minasensis has a wide geographical distribution. Previously, E. minasensis was found to cause clinical ehrlichiosis in an experimentally infected calf. The type strain E. minasensis UFMG-EV was successfully isolated from Rhipicephalus microplus ticks and propagated in the tick embryonic cell line of Ixodes scapularis (IDE8). However, the isolation and propagation of E. minasensis strains from cattle has remained elusive. In this study, the E. minasensis strain Cuiabá was isolated from an eight-month-old male calf of Holstein breed that was naturally infected with the bacterium. The calf presented clinical signs and hematological parameters of bovine ehrlichiosis. The in vitro culture of the agent was established in the canine cell line DH82. Ehrlichial morulae were observed using light and electron microscopy within DH82 cells. Total DNA was extracted, and the full genome of the E. minasensis strain Cuiabá was sequenced. A core-genome-based phylogenetic tree of Ehrlichia spp. and Anaplasma spp. confirmed that E. minasensis is a sister taxa of E. canis. A comparison of functional categories among Ehrlichia showed that E. minasensis has significantly less genes in the 'clustering-based subsystems' category, which includes functionally coupled genes for which the functional attributes are not well understood. Results strongly suggest that E. minasensis is a novel pathogen infecting cattle. The epidemiology of this Ehrlichia deserves further attention because these bacteria could be an overlooked cause of tick-borne bovine ehrlichiosis, with a wide distribution.

3.
Article in English | LILACS-Express | VETINDEX | ID: biblio-1457707

ABSTRACT

Background: Brachiaria spp. is the main pasture for ruminant productions in Brazil, but the limiting factor for its use is the toxicity due to the presence of steroidal saponins. Chronic ingestion of Brachiaria spp. by cattle may cause liver changes such as fibrosis, bile duct proliferation and clusters of foamy macrophages in the hepatic parenchyma. The main objective of the present study was to evaluate the most frequent histological changes and their frequencies in livers collected in abattoirs in Brazil from beef cattle raised exclusively on Brachiaria spp. and compare them with those observed in animals kept in Andropogon spp. grass and native pastures in Rio Grande do Sul.Materials, Methods & Results: Liver samples without macroscopic changes were collected in abattoirs from 561 healthy Nelore and Nelore crossbred cattle raised in Brachiaria spp. pastures from Mato Grosso (MT), Mato Grosso do Sul (MS), Minas Gerais (MG) and Pará (PA) States. Liver samples from 84 Angus cattle (Bos taurus) kept on native pastures in Rio Grande do Sul (RS) and from 60 Nelore and Nelore crossbreed cattle raised in Andropogon spp. pastures in Tocantins State (TO) were collected as control. Semi-quantitative analysis of the histopathological changes were proceeded: (-) = no change; (+) = slight or discreet change; (++) = moderate change and (+++) = marked change. The main histopathological

4.
Article in English | VETINDEX | ID: vti-733520

ABSTRACT

Background: Brachiaria spp. is the main pasture for ruminant productions in Brazil, but the limiting factor for its use is the toxicity due to the presence of steroidal saponins. Chronic ingestion of Brachiaria spp. by cattle may cause liver changes such as fibrosis, bile duct proliferation and clusters of foamy macrophages in the hepatic parenchyma. The main objective of the present study was to evaluate the most frequent histological changes and their frequencies in livers collected in abattoirs in Brazil from beef cattle raised exclusively on Brachiaria spp. and compare them with those observed in animals kept in Andropogon spp. grass and native pastures in Rio Grande do Sul.Materials, Methods & Results: Liver samples without macroscopic changes were collected in abattoirs from 561 healthy Nelore and Nelore crossbred cattle raised in Brachiaria spp. pastures from Mato Grosso (MT), Mato Grosso do Sul (MS), Minas Gerais (MG) and Pará (PA) States. Liver samples from 84 Angus cattle (Bos taurus) kept on native pastures in Rio Grande do Sul (RS) and from 60 Nelore and Nelore crossbreed cattle raised in Andropogon spp. pastures in Tocantins State (TO) were collected as control. Semi-quantitative analysis of the histopathological changes were proceeded: (-) = no change; (+) = slight or discreet change; (++) = moderate change and (+++) = marked change. The main histopathological

5.
Article in English | VETINDEX | ID: vti-732321

ABSTRACT

Background: Brachiaria spp. is the main pasture for ruminant productions in Brazil, but the limiting factor for its use is the toxicity due to the presence of steroidal saponins. Chronic ingestion of Brachiaria spp. by cattle may cause liver changes such as fibrosis, bile duct proliferation and clusters of foamy macrophages in the hepatic parenchyma. The main objective of the present study was to evaluate the most frequent histological changes and their frequencies in livers collected in abattoirs in Brazil from beef cattle raised exclusively on Brachiaria spp. and compare them with those observed in animals kept in Andropogon spp. grass and native pastures in Rio Grande do Sul.Materials, Methods & Results: Liver samples without macroscopic changes were collected in abattoirs from 561 healthy Nelore and Nelore crossbred cattle raised in Brachiaria spp. pastures from Mato Grosso (MT), Mato Grosso do Sul (MS), Minas Gerais (MG) and Pará (PA) States. Liver samples from 84 Angus cattle (Bos taurus) kept on native pastures in Rio Grande do Sul (RS) and from 60 Nelore and Nelore crossbreed cattle raised in Andropogon spp. pastures in Tocantins State (TO) were collected as control. Semi-quantitative analysis of the histopathological changes were proceeded: (-) = no change; (+) = slight or discreet change; (++) = moderate change and (+++) = marked change. The main histopathological

6.
Article in English | VETINDEX | ID: vti-731302

ABSTRACT

Background: Brachiaria spp. is the main pasture for ruminant productions in Brazil, but the limiting factor for its use is the toxicity due to the presence of steroidal saponins. Chronic ingestion of Brachiaria spp. by cattle may cause liver changes such as fibrosis, bile duct proliferation and clusters of foamy macrophages in the hepatic parenchyma. The main objective of the present study was to evaluate the most frequent histological changes and their frequencies in livers collected in abattoirs in Brazil from beef cattle raised exclusively on Brachiaria spp. and compare them with those observed in animals kept in Andropogon spp. grass and native pastures in Rio Grande do Sul.Materials, Methods & Results: Liver samples without macroscopic changes were collected in abattoirs from 561 healthy Nelore and Nelore crossbred cattle raised in Brachiaria spp. pastures from Mato Grosso (MT), Mato Grosso do Sul (MS), Minas Gerais (MG) and Pará (PA) States. Liver samples from 84 Angus cattle (Bos taurus) kept on native pastures in Rio Grande do Sul (RS) and from 60 Nelore and Nelore crossbreed cattle raised in Andropogon spp. pastures in Tocantins State (TO) were collected as control. Semi-quantitative analysis of the histopathological changes were proceeded: (-) = no change; (+) = slight or discreet change; (++) = moderate change and (+++) = marked change. The main histopathological

7.
Article in English | VETINDEX | ID: vti-730598

ABSTRACT

Background: Brachiaria spp. is the main pasture for ruminant productions in Brazil, but the limiting factor for its use is the toxicity due to the presence of steroidal saponins. Chronic ingestion of Brachiaria spp. by cattle may cause liver changes such as fibrosis, bile duct proliferation and clusters of foamy macrophages in the hepatic parenchyma. The main objective of the present study was to evaluate the most frequent histological changes and their frequencies in livers collected in abattoirs in Brazil from beef cattle raised exclusively on Brachiaria spp. and compare them with those observed in animals kept in Andropogon spp. grass and native pastures in Rio Grande do Sul.Materials, Methods & Results: Liver samples without macroscopic changes were collected in abattoirs from 561 healthy Nelore and Nelore crossbred cattle raised in Brachiaria spp. pastures from Mato Grosso (MT), Mato Grosso do Sul (MS), Minas Gerais (MG) and Pará (PA) States. Liver samples from 84 Angus cattle (Bos taurus) kept on native pastures in Rio Grande do Sul (RS) and from 60 Nelore and Nelore crossbreed cattle raised in Andropogon spp. pastures in Tocantins State (TO) were collected as control. Semi-quantitative analysis of the histopathological changes were proceeded: (-) = no change; (+) = slight or discreet change; (++) = moderate change and (+++) = marked change. The main histopathological

8.
Article in English | VETINDEX | ID: vti-730384

ABSTRACT

Background: Brachiaria spp. is the main pasture for ruminant productions in Brazil, but the limiting factor for its use is the toxicity due to the presence of steroidal saponins. Chronic ingestion of Brachiaria spp. by cattle may cause liver changes such as fibrosis, bile duct proliferation and clusters of foamy macrophages in the hepatic parenchyma. The main objective of the present study was to evaluate the most frequent histological changes and their frequencies in livers collected in abattoirs in Brazil from beef cattle raised exclusively on Brachiaria spp. and compare them with those observed in animals kept in Andropogon spp. grass and native pastures in Rio Grande do Sul.Materials, Methods & Results: Liver samples without macroscopic changes were collected in abattoirs from 561 healthy Nelore and Nelore crossbred cattle raised in Brachiaria spp. pastures from Mato Grosso (MT), Mato Grosso do Sul (MS), Minas Gerais (MG) and Pará (PA) States. Liver samples from 84 Angus cattle (Bos taurus) kept on native pastures in Rio Grande do Sul (RS) and from 60 Nelore and Nelore crossbreed cattle raised in Andropogon spp. pastures in Tocantins State (TO) were collected as control. Semi-quantitative analysis of the histopathological changes were proceeded: (-) = no change; (+) = slight or discreet change; (++) = moderate change and (+++) = marked change. The main histopathological

9.
Acta sci. vet. (Impr.) ; 41: 01-06, 2013.
Article in Portuguese | LILACS-Express | VETINDEX | ID: biblio-1457143

ABSTRACT

Background: Breast cancer is the most common cause of cancer-related death in canine female. Vascular endothelial growth factor is a major inducer of angiogenesis and has marked expression in mammary tumoral cells. The role of vascular endothelial growth factor that assumed to be most potent angiogenesis factor is ambiguous in breast cancer. Studies suggest that VEGF expression is higher in malignant than in benign tumors, and its correlation with invasion and growth of canine mammary tumors and unfavorable outcomes. The aim of this study was to determine and compare the immunohistochemical expression of VEGF in malignant and benign mammary tumors in bitches. Materials, Methods & Results: Fifty five malignant tumors were analyzed and sixteen benign tumors of dogs using a polyclonal antibody to determine the expression of VEGF by a semi-quantitative method (based on the sum of the ratio and intensity of color of the tumor cells) and a quantitative (performed by means of a computerized image capture and analysis). The marker VEGF positivity was determined by the presence of at least one tumor cell with brown cytoplasmic staining clear and unambiguous. The VEGF expression was compared between each histopathological group by Microsoft Office Excel 2007 and PHStat2 statistical software, version 2.0. We used the chi-squared test (2 ), to detect differences in the expression scor


O Fator de Crescimento Endotelial Vascular (Vascular Endothelial Growth Factor - VEGF) é o principal indutor da angiogênese tumoral, processo complexo, dinâmico e essencial para o crescimento de tumores que estimula a proliferação celular endotelial, migração e formação do tubo capilar, sendo um dos fatores mais estudados na oncologia humana. [...]

10.
Acta sci. vet. (Online) ; 41: 01-06, 2013.
Article in Portuguese | VETINDEX | ID: vti-480202

ABSTRACT

Background: Breast cancer is the most common cause of cancer-related death in canine female. Vascular endothelial growth factor is a major inducer of angiogenesis and has marked expression in mammary tumoral cells. The role of vascular endothelial growth factor that assumed to be most potent angiogenesis factor is ambiguous in breast cancer. Studies suggest that VEGF expression is higher in malignant than in benign tumors, and its correlation with invasion and growth of canine mammary tumors and unfavorable outcomes. The aim of this study was to determine and compare the immunohistochemical expression of VEGF in malignant and benign mammary tumors in bitches. Materials, Methods & Results: Fifty five malignant tumors were analyzed and sixteen benign tumors of dogs using a polyclonal antibody to determine the expression of VEGF by a semi-quantitative method (based on the sum of the ratio and intensity of color of the tumor cells) and a quantitative (performed by means of a computerized image capture and analysis). The marker VEGF positivity was determined by the presence of at least one tumor cell with brown cytoplasmic staining clear and unambiguous. The VEGF expression was compared between each histopathological group by Microsoft Office Excel 2007 and PHStat2 statistical software, version 2.0. We used the chi-squared test (2 ), to detect differences in the expression scor


O Fator de Crescimento Endotelial Vascular (Vascular Endothelial Growth Factor - VEGF) é o principal indutor da angiogênese tumoral, processo complexo, dinâmico e essencial para o crescimento de tumores que estimula a proliferação celular endotelial, migração e formação do tubo capilar, sendo um dos fatores mais estudados na oncologia humana. [...]

11.
Acta sci. vet. (Impr.) ; 40(2): 01-04, 2012.
Article in Portuguese | LILACS-Express | VETINDEX | ID: biblio-1456993

ABSTRACT

Background: Malignant catarrhal fever is an infectious pan-systemic viral disease, worldwide distribuition and highly fatal. The disease is described in many ruminant species, manly bovine. The lesions produced by malignant catarrhal fever in catlle affect upper respiratory and digestive tracts, lymph nodes, brain, eyes, kidney and urinary bladder. Affected animals present fever, depression, ocular and nasal discharge, erosions and ulcerations in the respiratory tract, keratoconjunctivitis, lymph node enlargement, hemorrhagic enteritis, encephalitis and arteritis. Four viruses are described as agents implicated on malignant catarrhal fever, although in Brazil, only ovine herpesvirus-2 has been described. Malignant catarrhal fever occurs in several regions of Brazil, including Mato Grosso state, where it was already reported in its southern region. The aim of this study was to describe the occurrence of malignant catarrhal fever in cattle in the northern region of the state of Mato Grosso.Case: Two affected bovine were necropsied. Tissue fragments were fixed with buffered formalin 10% and the hematoxylineosin sections were submitted to histopathology examination. Bovine DNA samples were extracted from paraffin embedded tissue fragments and submitted to nested PCR detection of ovine herpesvirus-2. The disease affected two bovine, one became clinically sick in October 2009 and the


Background: Malignant catarrhal fever is an infectious pan-systemic viral disease, worldwide distribuition and highly fatal. The disease is described in many ruminant species, manly bovine. The lesions produced by malignant catarrhal fever in catlle affect upper respiratory and digestive tracts, lymph nodes, brain, eyes, kidney and urinary bladder. Affected animals present fever, depression, ocular and nasal discharge, erosions and ulcerations in the respiratory tract, keratoconjunctivitis, lymph node enlargement, hemorrhagic enteritis, encephalitis and arteritis. Four viruses are described as agents implicated on malignant catarrhal fever, although in Brazil, only ovine herpesvirus-2 has been described. Malignant catarrhal fever occurs in several regions of Brazil, including Mato Grosso state, where it was already reported in its southern region. The aim of this study was to describe the occurrence of malignant catarrhal fever in cattle in the northern region of the state of Mato Grosso.Case: Two affected bovine were necropsied. Tissue fragments were fixed with buffered formalin 10% and the hematoxylineosin sections were submitted to histopathology examination. Bovine DNA samples were extracted from paraffin embedded tissue fragments and submitted to nested PCR detection of ovine herpesvirus-2. The disease affected two bovine, one became clinically sick in October 2009 and the

12.
R. bras. Ci. Vet. ; 19(2)2012.
Article in Portuguese | VETINDEX | ID: vti-712789

ABSTRACT

A miosite eosinofílica é uma doença imunomediada que acomete cães e caracteriza-se pela produção de anticorpos direcionadoscontra as fibras que constituem os músculos responsáveis pela mastigação. Esse distúrbio apresenta-se nas formas aguda oucrônica. A aguda é a mais comumente encontrada e envolve pseudotrismo, podendo progredir até a impossibilidade da aberturabucal. A forma crônica se caracteriza por atrofia e necrose dos músculos mastigatórios. A dosagem sérica de creatina quinase(CK) e o exame histopatológico do músculo envolvido são importantes para estabelecer o diagnóstico e avaliar a eficácia notratamento realizado. A resposta à terapia e o prognóstico mostram-se melhores quando a doença é tratada em sua forma aguda.Este artigo relata um caso de miosite dos músculos mastigatórios em um canino, fêmea, da raça Shar-Pei, atendido e tratado noHospital Veterinário da Universidade Federal de Mato Grosso (HOVET/UFMT), com curso de cinco meses. A CK apresentavaseelevada e ao exame histopatológico constatou-se necrose coagulativa, multifocal moderada de fibras musculares, infiltradoperivascular composto por células fagocíticas e frequente regeneração muscular. O animal foi tratado com prednisona (2 mg/kg),uma vez ao dia (SID), durante 20 dias consecutivos e apresentou melhora considerável. Conclui-se que a miosite dos músculosmastigatórios do animal em questão era de caráter i

13.
Acta sci. vet. (Online) ; 40(2): 01-04, 2012.
Article in Portuguese | VETINDEX | ID: vti-480259

ABSTRACT

Background: Malignant catarrhal fever is an infectious pan-systemic viral disease, worldwide distribuition and highly fatal. The disease is described in many ruminant species, manly bovine. The lesions produced by malignant catarrhal fever in catlle affect upper respiratory and digestive tracts, lymph nodes, brain, eyes, kidney and urinary bladder. Affected animals present fever, depression, ocular and nasal discharge, erosions and ulcerations in the respiratory tract, keratoconjunctivitis, lymph node enlargement, hemorrhagic enteritis, encephalitis and arteritis. Four viruses are described as agents implicated on malignant catarrhal fever, although in Brazil, only ovine herpesvirus-2 has been described. Malignant catarrhal fever occurs in several regions of Brazil, including Mato Grosso state, where it was already reported in its southern region. The aim of this study was to describe the occurrence of malignant catarrhal fever in cattle in the northern region of the state of Mato Grosso.Case: Two affected bovine were necropsied. Tissue fragments were fixed with buffered formalin 10% and the hematoxylineosin sections were submitted to histopathology examination. Bovine DNA samples were extracted from paraffin embedded tissue fragments and submitted to nested PCR detection of ovine herpesvirus-2. The disease affected two bovine, one became clinically sick in October 2009 and the


Background: Malignant catarrhal fever is an infectious pan-systemic viral disease, worldwide distribuition and highly fatal. The disease is described in many ruminant species, manly bovine. The lesions produced by malignant catarrhal fever in catlle affect upper respiratory and digestive tracts, lymph nodes, brain, eyes, kidney and urinary bladder. Affected animals present fever, depression, ocular and nasal discharge, erosions and ulcerations in the respiratory tract, keratoconjunctivitis, lymph node enlargement, hemorrhagic enteritis, encephalitis and arteritis. Four viruses are described as agents implicated on malignant catarrhal fever, although in Brazil, only ovine herpesvirus-2 has been described. Malignant catarrhal fever occurs in several regions of Brazil, including Mato Grosso state, where it was already reported in its southern region. The aim of this study was to describe the occurrence of malignant catarrhal fever in cattle in the northern region of the state of Mato Grosso.Case: Two affected bovine were necropsied. Tissue fragments were fixed with buffered formalin 10% and the hematoxylineosin sections were submitted to histopathology examination. Bovine DNA samples were extracted from paraffin embedded tissue fragments and submitted to nested PCR detection of ovine herpesvirus-2. The disease affected two bovine, one became clinically sick in October 2009 and the

14.
Article in Portuguese | LILACS-Express | LILACS, VETINDEX | ID: biblio-1491478

ABSTRACT

A miosite eosinofílica é uma doença imunomediada que acomete cães e caracteriza-se pela produção de anticorpos direcionadoscontra as fibras que constituem os músculos responsáveis pela mastigação. Esse distúrbio apresenta-se nas formas aguda oucrônica. A aguda é a mais comumente encontrada e envolve pseudotrismo, podendo progredir até a impossibilidade da aberturabucal. A forma crônica se caracteriza por atrofia e necrose dos músculos mastigatórios. A dosagem sérica de creatina quinase(CK) e o exame histopatológico do músculo envolvido são importantes para estabelecer o diagnóstico e avaliar a eficácia notratamento realizado. A resposta à terapia e o prognóstico mostram-se melhores quando a doença é tratada em sua forma aguda.Este artigo relata um caso de miosite dos músculos mastigatórios em um canino, fêmea, da raça Shar-Pei, atendido e tratado noHospital Veterinário da Universidade Federal de Mato Grosso (HOVET/UFMT), com curso de cinco meses. A CK apresentavaseelevada e ao exame histopatológico constatou-se necrose coagulativa, multifocal moderada de fibras musculares, infiltradoperivascular composto por células fagocíticas e frequente regeneração muscular. O animal foi tratado com prednisona (2 mg/kg),uma vez ao dia (SID), durante 20 dias consecutivos e apresentou melhora considerável. Conclui-se que a miosite dos músculosmastigatórios do animal em questão era de caráter i

15.
Semina Ci. agr. ; 32(1): 333-338, 2011.
Article in Portuguese | VETINDEX | ID: vti-472451

ABSTRACT

Among mammals, the canids are most often infected by Leishmania sp. This paper reports the occurrence of Leishmania (Leishmania) infantum chagasi as well as the presence of intranuclear inclusions resembling viral particles of canine adenovirus-1 in a bush dog (Speothos venaticus) kept in captivity at the zoo of Federal University of Mato Grosso, central area of Brazil.  The report demonstrates the importance of wild canids as reservoirs of agents of veterinary and public health importance, and it emphasizes the necessity of further studies with these animals concerning their habits, behavior and sanitary management. 


Dentre os mamíferos, os canídeos são os mais frequentemente infectados por Leishmania sp. O presente artigo relata a ocorrência de Leishmania (Leishmania) infantum chagasi e inclusões intranucleares sugestivas de adenovírus canino 1, em cachorro vinagre (Speothos venaticus) mantido no zoológico da Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso. O relato demonstra a importância dos canídeos silvestres como reservatórios de agentes de importância veterinária e de saúde pública, reforçando a necessidade de estudos com esses animais acerca de seus hábitos, comportamento e manejo sanitário.

16.
Exp Toxicol Pathol ; 62(2): 191-5, 2010 Mar.
Article in English | MEDLINE | ID: mdl-19428229

ABSTRACT

Human alpha-mannosidosis results from alpha-mannosidase deficiency and progressive accumulation of mannose-rich oligosaccharides in lysosomes. Two days before Saanen goats were fed with Sida carpinifolia, alpha-mannosidase activity in leukocytes was 128+/-28 nmoles4-MU/h/mgprotein (first trial) and 104+/-6 nmoles4-MU/h/mgprotein (second trial). At day 5, after the introduction of S. carpinifolia diet, the alpha-mannosidase activity in leukocytes was significantly increased, both in the first (288+/-13 nmoles4-MU/h/mgprotein) and in the second trial (303+/-45 nmoles4-MU/h/mgprotein), and it returned to normal levels 2 days after the withdrawal of the plant from the diet (114+/-7 nmoles4-MU/h/mgprotein in first trial, and 108+/-25 nmoles4-MU/h/mgprotein in the second one). Plasma alpha-mannosidase activity decreased significantly 4 days after animal exposure to the S. carpinifolia diet (769+/-167 nmoles4-MU/h/ml) and returned to normal values 10 days after the withdrawal of the plant from the diet (1289+/-163 nmoles4-MU/h/ml). Thin-layer chromatography showed an abnormal excretion of oligosaccharides in urine as of day 2 after diet exposure, which persisted until one day after the withdrawal of the plant. Animals presented neurological clinical signs beginning at day 37 (in the first trial) and at day 25 (in the second trial) after being fed with the plant. The results obtained herein suggest that oligosaccharides observed in urine are a result of a decrease in alpha-mannosidase activity in plasma. S. carpinifolia seems to have other compounds that act on alpha-mannosidase enzyme in leukocytes in a competitive manner with swainsonine. The increase in alpha-mannosidase enzyme in leukocytes could be attributed to one of these compounds present in S. carpinifolia.


Subject(s)
Leukocytes/enzymology , Malvaceae/toxicity , alpha-Mannosidase/metabolism , Animals , Chromatography, Thin Layer , Diet , Goats , Leukocytes/drug effects , Male , Malvaceae/chemistry , Oligosaccharides/urine , Swainsonine/chemistry , Swainsonine/toxicity , alpha-Mannosidase/analysis
17.
Acta sci. vet. (Impr.) ; 37(4): 363-366, 2009.
Article in Portuguese | LILACS-Express | VETINDEX | ID: biblio-1456744

ABSTRACT

Devido à deficiência de dados sobre a prevalência da infecção em suínos pelo Circovírus suíno tipo 2 (PCV-2) no Brasil e no mundo, e do impacto econômico no sistema de produção de suínos, este trabalho teve como objetivo estimar a ocorrência do PCV-2 em suínos abatidos no Estado do Mato Grosso. Para isso, foi realizada a técnica da Reação em Cadeia da Polimerase (PCR), em que foram avaliadas 121 tonsilas e 147 linfonodos mesentéricos de suínos abatidos no estado durante o período de janeiro de 2006 a dezembro de 2007. De todas as amostras analisadas, 58,21% (156/268) dos animais abatidos e 92,31% (12/13) dos municípios estudados estavam infectados pelo PCV-2. Mesmo apresentando resultados de prevalência inferiores aos encontrados em outros estados brasileiros e em outros países, o presente trabalho concluiu que é relevante a ocorrência do PCV-2 nos rebanhos mato-grossenses. Isto demonstra a importância de adotar medidas de controle mais eficazes para evitar que ocorra a Síndrome Multissistêmica do Definhamento dos Suínos (SMDS), e, com isso, amenizar o impacto econômico em sistemas de produção de suínos decorrente da mesma.

18.
Acta sci. vet. (Online) ; 37(4): 363-366, 2009.
Article in Portuguese | VETINDEX | ID: vti-733014

ABSTRACT

Devido à deficiência de dados sobre a prevalência da infecção em suínos pelo Circovírus suíno tipo 2 (PCV-2) no Brasil e no mundo, e do impacto econômico no sistema de produção de suínos, este trabalho teve como objetivo estimar a ocorrência do PCV-2 em suínos abatidos no Estado do Mato Grosso. Para isso, foi realizada a técnica da Reação em Cadeia da Polimerase (PCR), em que foram avaliadas 121 tonsilas e 147 linfonodos mesentéricos de suínos abatidos no estado durante o período de janeiro de 2006 a dezembro de 2007. De todas as amostras analisadas, 58,21% (156/268) dos animais abatidos e 92,31% (12/13) dos municípios estudados estavam infectados pelo PCV-2. Mesmo apresentando resultados de prevalência inferiores aos encontrados em outros estados brasileiros e em outros países, o presente trabalho concluiu que é relevante a ocorrência do PCV-2 nos rebanhos mato-grossenses. Isto demonstra a importância de adotar medidas de controle mais eficazes para evitar que ocorra a Síndrome Multissistêmica do Definhamento dos Suínos (SMDS), e, com isso, amenizar o impacto econômico em sistemas de produção de suínos decorrente da mesma.

19.
Acta sci. vet. (Online) ; 37(4): 363-366, 2009.
Article in Portuguese | VETINDEX | ID: vti-732419

ABSTRACT

Devido à deficiência de dados sobre a prevalência da infecção em suínos pelo Circovírus suíno tipo 2 (PCV-2) no Brasil e no mundo, e do impacto econômico no sistema de produção de suínos, este trabalho teve como objetivo estimar a ocorrência do PCV-2 em suínos abatidos no Estado do Mato Grosso. Para isso, foi realizada a técnica da Reação em Cadeia da Polimerase (PCR), em que foram avaliadas 121 tonsilas e 147 linfonodos mesentéricos de suínos abatidos no estado durante o período de janeiro de 2006 a dezembro de 2007. De todas as amostras analisadas, 58,21% (156/268) dos animais abatidos e 92,31% (12/13) dos municípios estudados estavam infectados pelo PCV-2. Mesmo apresentando resultados de prevalência inferiores aos encontrados em outros estados brasileiros e em outros países, o presente trabalho concluiu que é relevante a ocorrência do PCV-2 nos rebanhos mato-grossenses. Isto demonstra a importância de adotar medidas de controle mais eficazes para evitar que ocorra a Síndrome Multissistêmica do Definhamento dos Suínos (SMDS), e, com isso, amenizar o impacto econômico em sistemas de produção de suínos decorrente da mesma.

20.
Acta sci. vet. (Online) ; 37(4): 363-366, 2009.
Article in Portuguese | VETINDEX | ID: vti-731573

ABSTRACT

Devido à deficiência de dados sobre a prevalência da infecção em suínos pelo Circovírus suíno tipo 2 (PCV-2) no Brasil e no mundo, e do impacto econômico no sistema de produção de suínos, este trabalho teve como objetivo estimar a ocorrência do PCV-2 em suínos abatidos no Estado do Mato Grosso. Para isso, foi realizada a técnica da Reação em Cadeia da Polimerase (PCR), em que foram avaliadas 121 tonsilas e 147 linfonodos mesentéricos de suínos abatidos no estado durante o período de janeiro de 2006 a dezembro de 2007. De todas as amostras analisadas, 58,21% (156/268) dos animais abatidos e 92,31% (12/13) dos municípios estudados estavam infectados pelo PCV-2. Mesmo apresentando resultados de prevalência inferiores aos encontrados em outros estados brasileiros e em outros países, o presente trabalho concluiu que é relevante a ocorrência do PCV-2 nos rebanhos mato-grossenses. Isto demonstra a importância de adotar medidas de controle mais eficazes para evitar que ocorra a Síndrome Multissistêmica do Definhamento dos Suínos (SMDS), e, com isso, amenizar o impacto econômico em sistemas de produção de suínos decorrente da mesma.

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