Effect of Crude Glycerin in the Feed of Lactating Goats on Concentrations of Essential and Toxic Metals in Serum, Urine, Milk, and Artisanal "Coalho" Cheese.

Goat farming is concentrated in semi-arid and tropical regions in Brazil. From 2006 to 2017, the number of goats sold in the country increased by 65.7%. The dairy products from these animals present higher digestibility, high vitamin A and B content, hypoallergenicity, and less lactose compared to dairy products from cows, in addition to having a higher sales value. Since corn and soybean meal generate an expense for feed management, crude glycerin, originating from the manufacture of biodiesel, has been studied as an energy substitute. However, this product contains heavy metals, posing risks to animal and human health. Few data are available on trace elements in biological samples and products derived from goats' milk with the dietary introduction of glycerin. The objective was to quantify aluminum (Al), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), molybdenum (Mo), nickel (Ni), lead (Pb), and zinc (Zn) in the serum, urine, milk, and artisanal "coalho" cheese of dairy goats fed different levels of crude glycerin in feed management. In total, 16 Saanen goats were selected, lactating females, that were distributed in four Latin squares and randomly treated with different levels of crude glycerin (0, 5, 10, and 15%). After the end of each experiment cycle, serum, urine, milk, and artisanal "coalho" cheese samples were collected at the four moments. The samples were submitted to digestion assisted by microwave radiation. The multi-element analysis was carried out using inductively coupled plasma optical emission spectrometry (ICP OES). There was no influence of crude glycerin levels replacing corn in serum, milk, urine, and artisanal "coalho" cheese. The serum concentration of the metals Cu, Fe, Zn, and Mn; urine concentrations of Cu, Zn, Mn, Mo, and Cr; and milk and artisanal "coalho" cheese concentrations of Cu, Zn, and Mn remained within the standards found in the literature. The inclusion of 5 to 15% of crude glycerin, derived from cotton oil, in the diets of dairy goats in partial replacement of corn, does not alter the concentration of essential and toxic metals in serum, urine, milk, and artisanal "coalho" cheese and does not, therefore, present a risk factor for intoxication.

Cactus Cladodes Opuntia or Nopalea and By-Product of Low Nutritional Value as Solutions to Forage Shortages in Semiarid Areas.

We aimed to evaluate the effect of the cactus cladodes Nopalea cochenillifera (L). Salm-Dyck. (NUB) and cactus cladodes Opuntia stricta (Haw.) Haw. (OUB), both combined with sugarcane bagasse (SB) plus urea, Tifton hay (TH), corn silage (CS), and sorghum silage (SS) plus urea on nutrient intake and digestibility, ruminal dynamics, and parameters. Five male sheep, fistulated in the rumen, were assigned in a 5 × 5 Latin square design. The NUB provided a higher intake of dry matter (DM) and any nutrients than SS. TH provided larger pools of DM and iNDF. The OUB and CS provided a higher DM degradation. CS provided a higher NDF degradation rate. OUB provided a lower ruminal pH. Depending on the collection time, the lowest pH value was estimated at 3.79 h after the morning feeding. There was an interaction between treatments and collection time on VFA concentrations. Due to the high degradation rate, greater energy intake, less change in rumen pH, greater volatile fatty acid production, and feasibility, we recommend using cactus associated with sugarcane bagasse plus urea in sheep diets.

Palatability and nycterohemeral patterns of ingestive behavior of sheep fed different roughage sources.

The aim of this study was to evaluate the nycterohemeral patterns of ingestive behavior at 24, 48, and 72 h (Trial I) of sheep fed different roughages and the palatability index (Trial II) for sheep and goats fed different roughages used in a semiarid region. In the study, five sources of roughage were evaluated, namely: (1) Nopalea cochenillifera (L.) Salm-Dyck cladodes + urea + sugarcane bagasse (NUB); (2) Opuntia stricta (Haw.) Haw cladodes + urea + sugarcane bagasse (OUB); (3) Tifton hay; (4) corn silage; and (5) sorghum silage. Five Soinga sheep were used and arranged in a 5 × 5 Latin square design in Trial I. Six female Santa Ines sheep and six female Saanen goats were used to study the preferences for different roughages in Trial II, and three tests were carried out to determine the palatability index, comparing two roughage types at a time: test 1 - corn silage versus Nopalea cladodes + urea + sugarcane bagasse (NUB); test 2 - corn silage versus Opuntia cladodes + urea + sugarcane bagasse (OUB); and test 3 - NUB versus OUB. The highest dry matter (DM) intake was for diets composed by NUB. Feeding time (min/d) was higher for sorghum silage (291 min d-1), while rumination time was higher (P < 0.05) for corn silage (604.0 min d-1), sorghum silage (561.7 min d-1), and Tifton hay (529.7 min d-1). No difference (P > 0.05) was observed for feeding, rumination, and idleness time between species (sheep and goats), and there was no roughage × species interaction (P > 0.05). The palatability indexes were 76 and 77% (tests 1 and 2) for NUB and OUB, respectively. Sheep fed with roughage containing cactus cladodes associated with urea and sugarcane bagasse spent less time on ingestion and rumination activities. The ingestive behavior can be evaluated in a period of 48 h, using instantaneous scanning.

Nutritive Value, In Vitro Fermentation, and Methane Production of Cactus Cladodes, Sugarcane Bagasse, and Urea.

This study evaluated the effect of different roughages fed to sheep on nutrient and water intake, ingestive behavior, nitrogen balance, microbial protein synthesis, fermentation parameters, and methane production using an in vitro gas production system. The treatments consisted of five diets: cactus cladodes Nopalea (NUB) and Opuntia (OUB), both with the addition of sugarcane bagasse (SB) and urea/ammonium sulfate (urea/as); Tifton hay (TH); corn silage (CS); and sorghum silage (SS), also with added urea/as. The NUB provided greater (p ≤ 0.03) intakes of dry matter (1024 g/d), digestible organic matter (670 g/d), and crude protein (161 g/d) than those on the SS. The NUB provided greater (p < 0.01) dietary water intake (3023 g/d) than TH. The time spent on rumination was shorter (p < 0.01), and the idle time was longer in animals fed NUB and OUB than TH and CS. Microbial protein synthesis was not affected (p = 0.27). The final pH (6.4) of the incubation fluid and the concentration of NH3-N (39.05 mg/dL) were greater for NUB and OUB. Ruminal parameters and methane production were little or not affected by tested forages. We recommend using cactus cladodes in combination with sugarcane bagasse and urea/as in sheep diets.

Prediction of carcass composition through measurements in vivo and measurements of the carcass of growing Santa Inês sheep.

In vivo and carcass measurements were evaluated to predict carcass physical and chemical composition and to list the measurements that best fit the prediction of the composition of growing Santa Inês sheep carcasses. Thirty-three animals were used to measure the loin eye area by ultrasound in vivo (LEAu) and in the carcass. We used 39 animals for biometric measurement in vivo and 42 sheep for morphometric measurement in the carcass. For the physical and chemical compositions of carcasses, dissection of the half left carcass was carried out in 42 animals. The data were submitted to Pearson's correlation analysis and t test. Simple and multiple linear regressions were performed using a stepwise procedure. All correlations between in vivo measurements and the physical and chemical compositions of carcasses (in kg) were significant, except for LEAu. Biometric measurements and hot (HCW) and cold (CCW) carcass weights were considered as predictors of the carcasses' physical and chemical compositions. Slaughter body weight (SBW) was the variable that most influenced the equations in the assessment of in vivo measurements and HCW and CCW most influenced the equations for measurements on carcasses. Biometric measurements of Santa Inês sheep can be used together with the SBW to estimate the physical and chemical compositions of carcasses, with emphasis on body compactness index, breast width, wither height, and croup height. The morphometric measurements can be used together with carcass weight to estimate the physical and chemical compositions of carcasses, with emphasis on croup width, carcass compactness index, croup perimeter, external and internal carcass lengths, chest width, and leg length and perimeter. The HCW can be used to predict the physical and chemical composition of carcasses without affecting the accuracy of the prediction model.

Effects of additives in wet brewery residue silage on lamb carcass traits and meat quality.

The objective was to evaluate the use of wet brewery residue (WBR) silage additives on carcass characteristics and sheep meat quality. Thirty-two Santa Inês male sheep uncastrated with initial body weight of 22.61 ± 7.2 kg were allocated to a completely randomized design with four treatments: (1) WBR silage without additive (WBRS), (2) WBR silage with milled corn (WBRS + MC), (3) WBR silage with wheat bran (WBRS + WB), and (4) WBR silage with cassava flour (WBRS + CF) and eight replicates. WBRS + WB resulted in lower cold carcass weight than WBRS + CF; however, this reduction was not sufficient to alter the carcass commercial yield or loin-eye area. The leg cut of animals fed WBRS + WB showed less value than those animals fed with WBRS + CS. The meat lightness of WBRS was higher that of WBRS + MC, WBRS + WB, and WBRS + CF. The cooking loss for WBRS + WB was less than those animals fed with WBRS + CS. However, meat protein, meat cholesterol, and shear force were similar among treatments (17.69%, 42.46 mg/100 g of meat, and 2.48 kgf/cm2, respectively). The use of additives in wet brewery residue silage does not improve carcass characteristics or the quality of sheep meat, and it is therefore recommended to use WBR silage without additives.

Performance of growing lambs supplemented with ground licuri (Syagrus coronata).

OBJECTIVE: The aim of this study was to determine the effect of dietary ground licuri on lamb performance. METHODS: Forty male lambs were used in a completely randomized design to test the effects of 0, 5, 10, and 15 g/kg of ground licuri added to diets. The trial lasted for 75 days. Intake, digestibility, physically effective neutral detergent fiber, and chewing activity were estimated. Blood samples were taken on day 45 to determine the concentrations of glucose, urea, nonesterified fatty acids, and triglycerides. Average daily gain (ADG) were determined on the last day of the experimental trial. RESULTS: Licuri inclusion markedly increased dietary neutral detergent fiber and ether extract (EE) content, but it decreased dry matter (DM) intake. However, the intake and digestibility of EE linearly increased. The ADG decreased linearly (p<0.05) with licuri inclusion. Licuri had no effect (p>0.05) on the concentrations of blood metabolites; however, blood urea increased (p<0.05), while serum glucose decreased (p<0.05). CONCLUSION: The physically effective fiber of ground licuri is similar to Tyfton hay and licuri inclusion decreases lamb performance due to a decreased in DM intake.

Cactus cladodes associated with urea and sugarcane bagasse: an alternative to conserved feed in semi-arid regions.

The objective of this study was to evaluate the nutritional value of different roughage sources as an exclusive feed for goats and sheep from the determination of nutrient intake and digestibility. Five goats and five sheep were used and arranged in a double 5 × 5 Latin square design. Treatments consisted of cactus Nopalea cochenillifera (L.) Salm-Dyck cladodes (Nopalea) + urea + sugarcane bagasse (NUB), cactus Opuntia stricta (Haw.) Haw cladodes (Opuntia) + urea + sugarcane bagasse (OUB), Tifton hay (TH), maize silage (MS), and forage sorghum silage (SS). Dry matter (DM) and crude protein (CP) intakes of NUB were greater than SS (0.620 and 0.058 versus 0.416 and 0.040 kg/day). Metabolizable energy (ME) intake was higher for NUB (1.52 Mcal/day). The DM digestibility did not change between the roughages (59%). The NUB, OUB, and MS organic matter digestibility (OMD; 62.4%) were greater than that of SS (57.4%). The roughage consisting of cactus cladodes associated with urea and sugarcane bagasse presented a greater nutritional value, similar to MS and TH, and higher than SS. Cactus cladodes associated with urea and sugarcane bagasse are recommended as an alternative to conserved feed.

Effects of Saccharomyces cerevisiae and monensin on digestion, ruminal parameters, and balance of nitrogenous compounds of beef cattle fed diets with different starch concentrations.

This study was carried out aiming to evaluate the effects of yeast or monensin supplementation on dry matter intake, nutrients digestibility, ruminal volatile fatty acids profile, ruminal pH and ammonia concentration, microbial protein synthesis, and the balance of nitrogen compounds of cattle fed high concentrate diet (80 % dry matter (DM) basis) with two different levels of starch. Eight crossbred beef steers fitted with rumen cannula were assigned to two simultaneous 4 × 4 Latin squares arranged in a 4 × 2 factorial design. Two different starch levels (23 and 38 % of DM) were assigned to each Latin square, independently. Within each Latin square, four treatments were randomly assigned to the experimental animals (control; monensin; 1-g yeast [1 g/100 kg body weight (BW)/day] treatment; and 2.5-g yeast [2.5 g/100 kg BW/day] treatment). Feed additives did not influence ruminal pH (P > 0.05). Total ruminal volatile fatty acids (VFA) concentration was greater (P<0.05) in the diet with the lowest starch level. Similarly, monensin and 1-g yeast treatments resulted in greater (P<0.05) VFA concentration in the rumen. Monensin inclusion in the diet with the highest starch level led to a decrease (P<0.05) in lactate concentration in the rumen. However, acetate levels were increased (P<0.05) by the inclusion of 1 g of yeast in the diet with lowest starch level. Ruminal concentrations of propionate and butyrate, and ammonia-N were not influenced (P>0.05) by none of the additives evaluated. However, propionate concentration was greater (P<0.05) in the low-starch diets. Low-starch diets resulted in lower ruminal ammonia-N concentration and greater neutral detergent fiber digestibility (P<0.05). The excretion of urinary nitrogenous compounds, purine derivatives, synthesis of microbial protein, microbial efficiency, and balance of nitrogenous compounds were not affected by treatments evaluated (P>0.05). Monensin or yeast inclusion in high concentrate beef cattle diets in tropical regions as in Brazil is not justified by do not alter nutrient digestibility, nitrogen balance, and main ruminal parameters.

Performance and meat quality traits of beef heifers fed with two levels of concentrate and ruminally undegradable protein.

The effects of two levels of concentrate and ruminally undegradable protein (RUP) on performance, intake, digestibility, carcass characteristics, meat quality traits, and commercial cuts yield were assessed. Twenty crossbred heifers (240 kg average body weight) were used. At the beginning of the trial, four animals were slaughtered as reference group and the 16 remaining animals were randomly assigned to four treatments, in a 2 × 2 factorial design: two levels of concentrate (40% and 80%, dry matter (DM) basis) and two levels of RUP (48.79% and 27.19% of CP). At the end of the trial, all the animals were slaughtered. There was no interaction (P > 0.05) between concentrate and RUP levels. Dry matter intake and nutrients digestibility was not affected (P > 0.05) by RUP level. Heifers fed the highest RUP level had greater (P < 0.05) average daily gain (ADG). Concentrate level did not affect (P > 0.05) DMI and ADG. Heifers fed diets with 80% concentrate had greater intake of TDN and EE, and lower intake of NDF (P < 0.05). The digestibilities of all nutrients, except the NDF, were greater (P < 0.05) for the 80% concentrate diets. The RUP levels did not affect (P > 0.05) the carcass characteristics and carcass gain composition. Heifers fed 80% concentrate diets had larger (P < 0.05) longissimus muscle area and lower gain of bones (P < 0.05). Concentrate level did not affect (P > 0.05) the composition of carcass gain. There was no effect (P > 0.05) of RUP and concentrate levels on meat quality traits and commercial cut yields.

Características estruturais de perfilhos vegetativos e reprodutivos em pastos diferidos de capim-braquiária / Structural characteristics of vegetative and reproductive tillers on diferred signalgrass pastures

Este trabalho foi desenvolvido com o objetivo de comparar as características estruturais de perfilhos vegetativos e reprodutivos em pastos de Brachiaria decumbens cv. Basilisk diferidos por 73, 95 e 116 dias. O delineamento experimental foi em blocos casualizados, com três repetições, no esquema de parcelas subdivididas. O período de diferimento não influenciou (P>0,10) o número de perfilhos vegetativos (PV) e incrementou (P0,10) pelo período de diferimento. Os PR possuíram (P<0,05) maior comprimento do pseudocolmo, maior NFM e menor NFV do que o PV. Os PR possuíram maiores (P<0,05) percentuais de colmo e folha morta, bem como menor percentual de folha viva (P<0,05) do que os PV. O PV possui melhor composição morfológica do que o PR. O período de diferimento altera as características estruturais e piora a composição morfológica dos perfilhos em pastos diferidos de Brachiaria decumbens cv. Basilisk

The aim of this work was to compare the structural characteristics of vegetative and reproductive tillers on Brachiaria decumbens cv. Basilisk pastures diferred for 73, 95 and 116 days. Randomized block design in a split plot scheme with three replications was used. The stockpiling period did not influence (P>0.10) the number of vegetative tillers (VT) but increased (P0.10) by the deferring period. The RT revealed (P<0.05) a higher pseudostem length, higher number of dead leaves (NDL) and lower NLL when compared to VT. RT showed (P<0.05) higher percentages of stem and dead leaf as well as a lower percentage of living leaf (P<0.05) than VT. VT showed a better morphological composition than RT. The deferring period alters the structural characteristics and worsens the morphological composition of tillers on deferred Brachiaria decumbenscv. Basilisk pastures.

Características estruturais de perfilhos vegetativos e reprodutivos em pastos diferidos de capim-braquiária / Structural characteristics of vegetative and reproductive tillers on diferred signalgrass pastures

Este trabalho foi desenvolvido com o objetivo de comparar as características estruturais de perfilhos vegetativos e reprodutivos em pastos de Brachiaria decumbens cv. Basilisk diferidos por 73, 95 e 116 dias. O delineamento experimental foi em blocos casualizados, com três repetições, no esquema de parcelas subdivididas. O período de diferimento não influenciou (P>0,10) o número de perfilhos vegetativos (PV) e incrementou (P<0,10) o número de perfilhos reprodutivos (PR) (de 16 para 117 perfilhos/m2). Em pastos diferidos por até 95 dias, PR foi mais pesado (P<0,05) do que PV. Contrariamente ao PV, a relação entre número e peso de PR foi positiva (P<0,10). Verificou-se incremento linear (P<0,01 e P<0,10) do comprimento do pseudocolmo dos perfilhos em função do período de diferimento da pastagem. O número de folhas vivas (NFV) por perfilho não foi incrementado (P>0,10) pelo período de diferimento. Os PR possuíram (P<0,05) maior comprimento do pseudocolmo, maior NFM e menor NFV do que o PV. Os PR possuíram maiores (P<0,05) percentuais de colmo e folha morta, bem como menor percentual de folha viva (P<0,05) do que os PV. O PV possui melhor composição morfológica do que o PR. O período de diferimento altera as características estruturais e piora a composição morfológica dos perfilhos em pastos diferidos de Brachiaria decumbens cv. Basilisk(AU)

The aim of this work was to compare the structural characteristics of vegetative and reproductive tillers on Brachiaria decumbens cv. Basilisk pastures diferred for 73, 95 and 116 days. Randomized block design in a split plot scheme with three replications was used. The stockpiling period did not influence (P>0.10) the number of vegetative tillers (VT) but increased (P<0.10) the number of reproductive tillers (RT) (from 16 to 117 tillers/m2). On pastures diferred for up to 95 days, RT were heavier (P<0.05) than VT. Contrarily to VT, the relation between the number and weight of reproductive tillers was positive (P<0.10). A linear increase (P<0.01 and P<0.10) for tiller pseudostem length in relation to the diferring periods of the pasture was found. The number of living leaves (NLL) per tiller was not increased (P>0.10) by the deferring period. The RT revealed (P<0.05) a higher pseudostem length, higher number of dead leaves (NDL) and lower NLL when compared to VT. RT showed (P<0.05) higher percentages of stem and dead leaf as well as a lower percentage of living leaf (P<0.05) than VT. VT showed a better morphological composition than RT. The deferring period alters the structural characteristics and worsens the morphological composition of tillers on deferred Brachiaria decumbenscv. Basilisk pastures.(AU)