Temporomandibular disorder pain in older adolescents-young adults: Interrelationship with somatic burden, mental ill-being, and well-being.

OBJECTIVES: The associations of Temporomandibular disorder (TMD) pain with somatic symptoms, mental ill-being/distress, and well-being were explored, along with the mental correlates of TMD (TS) and somatic symptom (SS) burden in older adolescents-young adults. METHODS: TMD/somatic symptoms were appraised with the five quintessential symptoms (5Ts) of the DC/TMD/Somatic Symptom Scale-8, whereas mental distress/well-being was assessed with the Depression, Anxiety, Stress Scales-21/Scales of Psychological Well-being-18. Data were examined using Chi-square/non-parametric tests and multivariate analyses (α = .05). RESULTS: Among the 366 participants, 51.4%, 28.1%, 6.8%, and 13.7% had no TMD (NT), TMD pain (TP), TMD dysfunction (TD), and combined TMD (CT) respectively. Though mental distress varied substantially (CT, TP>NT, TD), no significant differences in well-being were discerned. SS burden, but not TS burden, was moderately correlated to distress. CONCLUSIONS: The prospect of TMD pain was increased by being female, depressed, and anxious but reduced by "positive relations with others" and "self-acceptance".

Stress and emotional distress: their associations with somatic and temporomandibular disorder-related symptoms.

Although Asians are posited to be predisposed to somatization, studies on the inter-relationships between emotional and physical symptoms in Asian youths are still limited. This study determined the associations between emotional, somatic, and Temporomandibular Disorder (TMD)-related symptoms in Asian youths. Youths were recruited from a local polytechnic and the prevalence/severity of emotional distress was ascertained with the Depression, Anxiety, and Stress Scale-21. Somatic and TMD-related symptoms were appraised with the Patient Health Questionnaire-15 and Fonseca Anamnestic Index. Data were analysed using non-parametric statistical and logistic regression analysis (p < 0.05). The study sample (n = 400; 209 females) had a mean age of 18.7 ± 1.7 years. Moderate-to-severe depression, anxiety, and stress were present in 31.0%, 36.8%, and 16.5% of the subjects respectively. Subjects with moderate-to-severe emotional distress had significantly higher somatic and TMD symptom scores (p < 0.001). Correlations between emotional and somatic/TMD symptom scores were moderately strong (rs = 0.46-0.61). Stepwise logistic regression indicated that moderate-to-severe somatization was associated with the female gender, anxiety, and stress, and moderate-to-severe TMDs were related to somatization and stress. Asian youths with elevated emotional distress experienced significantly higher levels of somatic and TMD-related symptoms. The stress construct appears to pose a greater risk for somatization and TMDs than depression and anxiety.

Somatic and temporomandibular disorder symptoms - Idioms of psychological distress in Southeast Asian youths.

OBJECTIVE: To evaluate the prevalence/severity of somatic and temporomandibular disorder (TMD) symptoms in Southeast Asian youths and determine their associations with psychological distress. METHODS: Demographic information, Patient Health Questionnaire-15 (PHQ-15), Fonseca Anamnestic Index (FAI), and Depression, Anxiety, Stress Scale-21 (DASS-21) responses were gathered electronically and analyzed using non-parametric statistical and logistic regression analysis (p < 0.05). RESULTS: Of 400 youths (mean age 18.7 ± 1.7 years; 52.3% females), 65.0%/47.0% reported somatic/TMD symptoms, and 10.5% had TMDs. Significant differences in psychological distress were observed among the varying severity of somatic/TMD symptoms. Correlations between PHQ-15/FAI and DASS-21 scores were weak to moderately strong (rs = 0.30-0.61). Stepwise logistic regression indicated that female gender, TMD symptoms, and stress were risk factors for somatic symptoms, while somatic symptoms and stress were probable factors for TMDs. CONCLUSION: Somatic and TMD symptoms are common in Southeast Asian youths and may be a manifestation of psychological distress.

Inter-relationships between pain-related temporomandibular disorders, somatic and psychological symptoms in Asian youths.

OBJECTIVE: This cross-sectional, observational study assessed the inter-relationships between painful TMDs, somatic and psychological symptoms in Asian youths. METHODS: Study subjects were recruited from a local Polytechnic. Pain-related TMDs were established with the TMD pain screener (TPS), while somatic and psychological symptoms were assessed with the Patient Health Questionnaire-15 (PHQ-15) and Depression, Anxiety and Stress Scales-21 (DASS-21). Demographic information, TPS, PHQ-15 and DASS-21 responses were gathered with the CDRSS system and analysed using the binomial-exact test, Mann-Whitney U test, Spearman's rho correlation and logistic regression analysis (P < .05). RESULTS: Data from a total of 400 participants were collected and examined. The "no TMDs" (NT) group comprised of 366 subjects (180 males; 186 females) with a mean age of 18.72 ± 1.49 years, while the "with TMDs" (WT) group consisted of 34 subjects (11 males; 23 females) aged 19.26 ± 1.89 years. Significant differences in median PHQ-15 and DASS-21 scores were observed between the NT and WT groups (P ≤ .007). The WT group was observed to have moderate somatic symptoms, moderate depression, moderate-to-severe anxiety and mild stress. For subjects with pain-related TMDs, correlation between somatic and psychological symptoms was moderate-to-strong (rs  = .55-.69). Stepwise logistic regression indicated that somatisation and anxiety were possible risk factors for painful TMDs with odds ratios of 1.08 and 1.07, respectively. CONCLUSIONS: The prevalence of pain-related TMDs was 8.5%. Subjects with painful TMDs had significantly higher levels of somatic and psychological distress. In addition, somatic symptoms were strongly correlated with anxiety and stress but less so with depression.

Functional, physical and psychosocial impact of Temporomandibular Disorders in adolescents and young adults

BACKGROUND: This community-based study investigated the functional, physical and psychosocial impact of Temporomandibular Disorders (TMDs) in adolescents and young adults. It also determined the discriminative capacity of a TMDs-specific oral health related quality of life (OHRQoL) instrument and compared three formats of appraising OHRQoL data. MATERIAL AND METHODS: Subjects were recruited from a local Polytechnic. The presence of TMDs was established with the Fonseca Anamnestic Index (FAI), whilst TMDs-specific OHRQoL was evaluated with the Oral Health Impact Profile-TMDs (OHIP-TMDs). Demographic information, FAI and OHIP-TMDs responses were gathered with an on-line questionnaire. Data was analysed using Mann-Whitney U-test, chi-square test and Spearman's rho correlation with significance level set at 0.05. RESULTS: Data from a total of 244 participants were compiled and examined. The "no TMDs" (NT) group consisted of 140 subjects (119 females; 21 males) with a mean age of 20.41 ± 3.29 years, while the "with TMDs" (WT) group composed of 104 subjects (88 females; 16 males) aged 19.82 ± 3.04 years. Significant differences in median severity scores were observed between subjects with and without TMDs for all OHIP-TMDs domains and total OHIP (p values < 0.001). For appraisal of extent and prevalence, significant differences were again observed (p values < 0.05) with the exception of the functional limitation and handicap domains. CONCLUSIONS: TMDs impacted physical and psychosocial well-being of adolescents and young adults. OHIP-TMDs, preferably appraised by severity, extent and prevalence, was able to discriminate between subjects with and without TMDs. It holds promise as a TMDs-specific OHRQoL instrument for epidemiological studies

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Innate Immune Response of Human Embryonic Stem Cell-Derived Fibroblasts and Mesenchymal Stem Cells to Periodontopathogens.

Periodontitis involves complex interplay of bacteria and host immune response resulting in destruction of supporting tissues of the tooth. Toll-like receptors (TLRs) play a role in recognizing microbial pathogens and eliciting an innate immune response. Recently, the potential application of multipotent stem cells and pluripotent stem cells including human embryonic stem cells (hESCs) in periodontal regenerative therapy has been proposed. However, little is known about the impact of periodontopathogens on hESC-derived progenies. This study investigates the effects of heat-killed periodontopathogens, namely, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, on TLR and cytokine expression profile of hESC-derived progenies, namely, fibroblasts (hESC-Fib) and mesenchymal stem cells (hESC-MSCs). Additionally, the serotype-dependent effect of A. actinomycetemcomitans on hESC-derived progenies was explored. Both hESC-Fib and hESC-MSCs constitutively expressed TLR-2 and TLR-4. hESC-Fib upon exposure to periodontopathogens displayed upregulation of TLRs and release of cytokines (IL-1ß, IL-6, and IL-8). In contrast, hESC-MSCs were largely nonresponsive to bacterial challenge, especially in terms of cytokine production. Further, exposure of hESC-Fib to A. actinomycetemcomitans serotype c was associated with higher IL-8 production than serotype b. In contrast, the hESC-MSCs displayed no serotype-dependent response. Differential response of the two hESC progenies implies a phenotype-dependent response to periodontopathogens and supports the concept of immunomodulatory properties of MSCs.

Bioactivity, physical and chemical properties of MTA mixed with propylene glycol.

OBJECTIVE: To investigate the physical (setting time, hardness, flowability, microstructure) and chemical (pH change, calcium release, crystallinity) properties and the biological outcomes (cell survival and differentiation) of mineral trioxide aggregate (MTA) mixed using different proportions of propylene glycol (PG) and water. MATERIAL AND METHODS: White MTA was mixed with different water/PG ratios (100/0, 80/20 and 50/50). Composition (XRD), microstructure (SEM), setting time (ASTM C266-13), flowability (ANSI/ADA 57-2000), Knoop hardness (100 g/10 s) and chemical characteristics (pH change and Ca2+ release for 7 days) were evaluated. Cell proliferation, osteo/odontoblastic gene expression and mineralization induced by MTA mixed with PG were evaluated. MTA discs (5 mm in diameter, 2 mm thick) were prepared and soaked in culture medium for 7 days. Next, the discs were removed and the medium used to culture dental pulp stem cells (DPSC) for 28 days. Cells survival was evaluated using MTS assay (24, 72 and 120 h) and differentiation with RT-PCR (ALP, OCN, Runx2, DSPP and MEPE) and alizarin red staining (7 and 14 days). Data were analysed using one-way ANOVA and Tukey's post-hoc analysis (a=0.05). RESULTS: The addition of PG significantly increased setting time, flowability and Ca2+ release, but it compromised the hardness of the material. SEM showed that 50/50 group resulted porous material after setting due to the incomplete setting reaction, as shown by XRD analysis. The addition of PG (80/20 and 50/50) was not capable to improve cell proliferation or to enhance gene expression, and mineralized deposition of DPSC after 7 and 14 days as compared to the 100/0. CONCLUSION: Except for flowability, the addition of PG did not promote further improvements on the chemical and physical properties evaluated, and it was not capable of enhancing the bioactivity of the MTA.

Bioactivity, physical and chemical properties of MTA mixed with propylene glycol

AbstractObjective To investigate the physical (setting time, hardness, flowability, microstructure) and chemical (pH change, calcium release, crystallinity) properties and the biological outcomes (cell survival and differentiation) of mineral trioxide aggregate (MTA) mixed using different proportions of propylene glycol (PG) and water.Material and Methods White MTA was mixed with different water/PG ratios (100/0, 80/20 and 50/50). Composition (XRD), microstructure (SEM), setting time (ASTM C266-13), flowability (ANSI/ADA 57-2000), Knoop hardness (100 g/10 s) and chemical characteristics (pH change and Ca2+ release for 7 days) were evaluated. Cell proliferation, osteo/odontoblastic gene expression and mineralization induced by MTA mixed with PG were evaluated. MTA discs (5 mm in diameter, 2 mm thick) were prepared and soaked in culture medium for 7 days. Next, the discs were removed and the medium used to culture dental pulp stem cells (DPSC) for 28 days. Cells survival was evaluated using MTS assay (24, 72 and 120 h) and differentiation with RT-PCR (ALP, OCN, Runx2, DSPP and MEPE) and alizarin red staining (7 and 14 days). Data were analysed using one-way ANOVA and Tukey’s post-hoc analysis (a=0.05).Results The addition of PG significantly increased setting time, flowability and Ca2+ release, but it compromised the hardness of the material. SEM showed that 50/50 group resulted porous material after setting due to the incomplete setting reaction, as shown by XRD analysis. The addition of PG (80/20 and 50/50) was not capable to improve cell proliferation or to enhance gene expression, and mineralized deposition of DPSC after 7 and 14 days as compared to the 100/0.Conclusion Except for flowability, the addition of PG did not promote further improvements on the chemical and physical properties evaluated, and it was not capable of enhancing the bioactivity of the MTA.