Multi-environment testing for G×E interactions and identification of high-yielding, stable, medium-duration pigeonpea genotypes employing AMMI, GGE biplot, and YREM analyses.

Pigeonpea [Cajanus cajan (L.) Millspaugh] is a widely grown pulse with high seed protein content that contributes to food and nutritional security in the Indian subcontinent. The majority of pigeonpea varieties cultivated in India are of medium duration (<180 days to maturity), which makes it essential for breeders to focus on the development of stable high-yielding varieties. The diverse agroecological regime in the Indian subcontinent necessitates an efficient multi-environment study by taking into consideration genotype (G) × environment (E) interaction (GEI) that has a significant impact on traits like grain yield (GY) in developing high-yielding and widely adaptable varieties. In the present study, 37 pigeonpea genotypes were evaluated during the 2021 rainy season at ARS Badnapur, ARS Tandur, BAU Ranchi, GKVK Bengaluru, and ICRISAT Patancheru. The GEI was significant on the grain yield (p < 0.01), and hence, genotype + genotype × environment (GGE) and additive main effects and multiplicative interaction (AMMI) biplots along with AMMI stability value (ASV) and yield relative to environmental maximum (YREM) statistics were used to identify stable high-yielding genotypes. The interaction principal component analysis 1 and 2 (IPC1 and IPC2) explained 40.6% and 23.3% variations, respectively. Based on the rankings of genotypes, G37 (ICPL 20205), G35 (ICPL 20203), G8 (ICPL 19404), G17 (ICPL 19415), and G9 (ICPL 19405) were identified as ideal genotypes. Discriminativeness vs. representativeness identified GKVK Bengaluru as an ideal environment for comprehensive evaluation of test genotypes. However, ICPL 19405 was identified as the potentially stable high-yielding genotype for further testing and release across the test environments based on its mean grain yield (1,469.30 kg/ha), least ASV (3.82), and low yield stability index (YSI) of 13.

Genomic patterns of selection in morphometric traits across diverse Indian cattle breeds.

This study seeks a comprehensive exploration of genome-wide selective processes impacting morphometric traits across diverse cattle breeds, utilizing an array of statistical methods. Morphometric traits, encompassing both qualitative and quantitative variables, play a pivotal role in characterizing and selecting livestock breeds based on their external appearance, size, and physical attributes. While qualitative traits, such as color, horn structure, and coat type, contribute to adaptive features and breed identification, quantitative traits like body weight and conformation measurements bear a closer correlation with production characteristics. This study employs advanced genotyping technologies, including the Illumina BovineSNP50 Bead Chip and next-generation sequencing methods like Reduced Representation sequencing, to identify genomic signatures associated with these traits. We applied four intra-population methods to find evidence of selection, such as Tajima's D, CLR, iHS, and ROH. We found a total of 40 genes under the selection signature, that were associated with morphometric traits in five cattle breeds (Kankrej, Tharparkar, Nelore, Sahiwal, and Gir). Crucial genes such as ADIPDQ, DPP6, INSIG1, SLC35D2 in Kankrej, LPL, ATP6V1B2, CDC14B in Tharparkar, HPSE2, PLAG1 in Nelore, PCSK1, PRKD1 in Sahiwal, and GNAQ, HPCAL1 in Gir were identified in our study. This approach provides valuable insights into the genetic basis of variations in body weight and conformation traits, facilitating informed selection processes and offering a deeper understanding of the evolutionary and domestication processes in diverse cattle breeds.

Development and evaluation of Fusarium wilt-resistant and high-yielding chickpea advanced breeding line, KCD 11.

Fusarium wilt (FW) is the most severe soil-borne disease of chickpea that causes yield losses up to 100%. To improve FW resistance in JG 11, a high-yielding variety that became susceptible to FW, we used WR 315 as the donor parent and followed the pedigree breeding method. Based on disease resistance and yield performance, four lines were evaluated in station trials during 2017-2018 and 2018-2019 at Kalaburagi, India. Further, two lines, namely, Kalaburagi chickpea desi 5 (KCD 5) and KCD 11, which possesses the resistance allele for a specific single-nucleotide polymorphism marker linked with FW resistance, were evaluated across six different locations (Bidar, Kalaburagi, Raichur, Siruguppa, Bhimarayanagudi and Hagari) over a span of 3 years (2020-2021, 2021-2022 and 2022-2023). KCD 11 exhibited notable performance, showcasing yield advantages of 8.67%, 11.26% and 23.88% over JG 11, and the regional checks Super Annigeri 1 (SA 1) and Annigeri 1, respectively, with enhanced FW resistance in wilt sick plot. Further, KCD 11 outperformed JG 11, SA 1 and Annigeri 1 in multi-location trials conducted across three seasons in the North Eastern Transition Zone, North Eastern Dry Zone, and North Dry Zones of Karnataka. KCD 11 was also tested in trials conducted by All India Coordinated Research Project on chickpea and was also nominated for state varietal trials for its release as a FW-resistant and high-yielding variety. The selected line is anticipated to cater the needs of chickpea growers with the dual advantage of yield increment and disease resistance.

Comparative evaluation of hand and rotary file systems on dentinal microcrack formation during pulpectomy procedure in primary teeth: an in vitro study.

PURPOSE: Pulpectomy can be used for the management of deep dentinal carious lesions in primary teeth which can be restored. Mechanical preparation of root canals can be performed using hand or NiTi rotary files. However, this may cause dentinal stress and consequently dentinal microcracks. Hence, the aim was comparative evaluation of hand and rotary file systems on dentinal microcrack formation during pulpectomy procedure in primary teeth. METHODS: 60 extracted primary molar teeth were selected comprising of 80 root canals. Simple random sampling was used to divide root canals into four groups (n = 20): Group A-Hedstrom file, Group B-Pro AF Baby Gold rotary, Group C-ProTaper Next rotary, and Group D-unprepared group. Assessment was conducted on presence or absence of microcracks using Chi square test (p < 0.05). RESULTS: The total number of microcracks in Group A: one (5%), Group B: four (20%), Group C: nine (45%) and Group D: zero (0%) which was statistically significant (p = 0.002). At cervical third, the number of microcracks seen with Group A: one (5%), Group B: zero (0%), Group C: five (25%) and Group D: zero (0%) (p = 0.005). At the middle third, the number of microcracks seen in Group A: zero (0%), Group B: four (20%), Group C: four (20%) and Group D: zero (0%) (p = 0.029). CONCLUSION: The study concluded that dentinal microcracks are formed with both hand and rotary file systems in primary teeth. ProTaper Next showed significantly higher number of microcracks, followed by ProAF Baby Gold and H files.

Excited state spectroscopy and spin splitting in single layer MoS2 quantum dots.

Semiconducting transition metal dichalcogenides (TMDCs) are very promising materials for quantum dots and spin-qubit implementation. Reliable operation of spin qubits requires the knowledge of the Landé g-factor, which can be measured by exploiting the discrete energy spectrum on a quantum dot. However, the quantum dots realized in TMDCs are yet to reach the required control and quality for reliable measurement of excited state spectroscopy and the g-factor, particularly in atomically thin layers. Quantum dot sizes reported in TMDCs so far are not small enough to observe discrete energy levels on them. Here, we report on electron transport through discrete energy levels of quantum dots in a single layer MoS2 isolated from its environment using a dual gate geometry. The quantum dot energy levels are separated by a few (5-6) meV such that the ground state and the first excited state transitions are clearly visible, thanks to the low contact resistance of ∼700 Ω and relatively low gate voltages. This well-resolved energy separation allowed us to accurately measure the ground state g-factor of ∼5 in MoS2 quantum dots. We observed a spin-filling sequence in our quantum dots under a perpendicular magnetic field. Such a system offers an excellent testbed to measure the key parameters for evaluation and implementation of spin-valley qubits in TMDCs, thus accelerating the development of quantum systems in two-dimensional semiconducting TMDCs.

Ethical Responsibilities for Companies That Process Personal Data.

It has become increasingly difficult for individuals to exercise meaningful control over the personal data they disclose to companies or to understand and track the ways in which that data is exchanged and used. These developments have led to an emerging consensus that existing privacy and data protection laws offer individuals insufficient protections against harms stemming from current data practices. However, an effective and ethically justified way forward remains elusive. To inform policy in this area, we propose the Ethical Data Practices framework. The framework outlines six principles relevant to the collection and use of personal data-minimizing harm, fairly distributing benefits and burdens, respecting autonomy, transparency, accountability, and inclusion-and translates these principles into action-guiding practical imperatives for companies that process personal data. In addition to informing policy, the practical imperatives can be voluntarily adopted by companies to promote ethical data practices.

Comparative evaluation of shear bond strength of three different glass ionomer cement (conventional, zirconium-reinforced and advanced glass hybrid) in primary molars: an in vitro study.

PURPOSE: The success of restorative materials is largely dependent on their capacity to adhere to the tooth structure and withstand the various forces present in the oral cavity. So, the aim of present study was to evaluate and compare the shear bond strength (SBS) of Type IX Glass Ionomer Cement (GIC), Zirconomer, and Gold Label Hybrid GIC in primary molars. METHODS: Thirty primary molars were selected based on inclusion and exclusion criteria. The molars were polished to provide a flat dentin surface after being inserted in auto polymerizing acrylic resin. The samples were randomly divided into three groups, equally and were bonded to GIC. On the dentin surface, restoration cylinders were made using a plastic mould that had an internal diameter and height of 5 mm and 3 mm, respectively. The cement was manipulated according to the manufacturer's directions through the plastic mould. Then, the samples were stored at room temperature for 10 days to mimic oral conditions. The Universal Testing Machine was used to test SBS. One-way ANOVA and the post hoc Tukey test were used to statistically assess the collected data. RESULTS: A statistically significant difference was found in all three groups (p < 0.01), with Zirconomer demonstrating highest SBS, followed by Type IX GIC and Gold Label Hybrid GIC. CONCLUSION: The SBS value of Zirconomer was better when compared to Type IX GIC and Gold Label Hybrid GIC.

Amorphous nickel tungstate films prepared by SILAR method for electrocatalytic oxygen evolution reaction.

Development of electrocatalyst using facile way from non-noble metal compounds with high efficiency for effective water electrolysis is highly demanding for production of hydrogen energy. Nickel based electrocatalysts were currently developed for electrochemical water oxidation in alkaline pH. Herein, amorphous nickel tungstate (NiWO4) was synthesized using the facile successive ionic layer adsorption and reaction method. The films were characterized by X-ray diffraction, Raman spectroscopy, Fourier transfer infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and transmission electron microscopy techniques. The electrochemical analysis showed 315 mV of overpotential at 100 mA cm-2 with lowest Tafel slope of 32 mV dec-1 for oxygen evolution reaction (OER) making films of NiWO4 compatible towards electrocatalysis of water in alkaline media. The chronopotentiometry measurements at 100 mA cm-2 over 24 h showed 97% retention of OER activity. The electrochemical active surface area (ECSA) of NW120 film was 25.5 cm-2.

Diagnostic performance of the BHBCheck ß-hydroxybutyrate meter for hyperketonaemia in Indian cows and buffaloes.

The objective of the study was to evaluate the diagnostic performance of the electronic handheld BHBCheck meter (BHM) (PortaCheck, Inc., USA) to determine whole blood, plasma and serum ß-hydroxybutyrate (BHB) against serum BHB determined using reference laboratory method of Randox D-3 Hydroxybutyrate Ranbut assay (RSM) in Indian dairy cows and buffaloes. Blood samples were collected by puncturing coccygeal vessels for determining whole blood, serum and plasma BHB using BHM and serum BHB using RSM from 217 cows (Gir breed; median 42 DIM and 3rd lactation) and 223 buffaloes (non-descript; median 39 DIM and 3rd lactation) from nearby herds. The Pearson's correlation between whole blood (0.988; 0.987), plasma (0.985; 0.983) and serum (0.985; 0.983) BHB determined using the BHM and serum BHB determined with the RSM in Indian cows and buffaloes, respectively, were significant. Bland-Altman plot demonstrated an excellent agreement between whole blood, plasma and serum BHB determined with BHM, against the serum BHB determined with RSM in Indian cows and buffaloes, respectively. For hyperketonaemia with reference serum BHB cut-off values ≥ 1.2 and 1.4 mmol/L determined with RSM, it recorded optimized BHB thresholds, sensitivity and specificity for whole blood (≥ 0.9 to 1.0 mmol/L; 91 to 95% and 88 to 98%), plasma (≥ 0.9 to 1.0 mmol/L; 91 and 100%) and serum (≥ 0.9 to 1.0 mmol/L; 92 to 100% and 85 to 94%) with BHM in cows and buffaloes, respectively. In conclusion, BHB determined with BHM demonstrated an excellent correlation, agreement and test characteristics with BHB determined with RSM and hence can accurately determine whole blood, plasma and serum BHB in cows and buffaloes.

Cold Plasma: Clean Technology to Destroy Pathogenic Micro-organisms.

Atmospheric pressure cold plasma is a promising technology in fighting pathogenic micro-organisms. In times of Covid-19 pandemic, it was decided to modify two types of cold plasma devices to study their effectiveness in the killing of pathogenic micro-organisms. These studies have shown that both the devices are efficient in this purpose. While pencil like microwave based device can destroy Aeromonas bacteria and its bacteriophage from 6 cm distance in 2 min, the larger (~ 40 cm2) RF plasma based device could do the similar killing ability for the larger possible area in 4 min. Optical Emission Spectroscopy (OES) studies revealed that both these devices produce OH radicals which helped in the destruction of both bacteria and its bacteriophage. With suitable modifications, these devices, especially the larger area device may even be implemented for the elimination of Covid-19 affected wards of hospital without using any sensitive chemical process.

Isophthaloyl-Based Selective Fluorescence Receptor for Zn (II) Ion in Semi-Aqueous Medium.

A novel Isophthaloyl-based symmetrical (12E,21E)-N1',N3'-bis(2-hydroxybenzylidene) isophthalohydrazide, receptor (1) was synthesized and characterized using various spectroscopic technique. The reorganization ability of receptor (1) was evaluated in semi-aqueous medium and shows significant enhancement in fluorescence intensity for Zn (II) ion over various metal ions in CH3CN:H2O (1:1, v/v). The 1:2 binding stoichiometry between receptor (1) and Zn (II) ion was established using Job's plot and the proposed complex structure was calculated by applying Density Functional Theory (DFT) method. The binding constant (Ka) of receptor (1) with Zn (II) ion was established with the Benesi-Hildebrand plot, Scatchard and Connor's plot and the values are 1.00 × 104 M-1, 1.05× 104 M-1 and 1.05× 104 M-1 respectively. The limit of detection (LOD) and limit of quantification (LOQ) of receptor (1) and Zn (II) ion was 0.292 µM and 0.974 µM respectively. The binding mode was due to photo-induced electron transfer (PET) and the coordination of Zn (II) ion with C = N hydroxyl group of receptor (1). Electrochemical analysis of metal free receptor (1) and with Zn (II) ion also confirmed the formation of complex.

Selective modification of fluciclovine (18F) transport in prostate carcinoma xenografts.

We investigated if previously demonstrated inhibition of fluciclovine (18F) in vitro could be replicated in a PC3-Luc xenograft mouse model. Following intratumoral injection of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), alpha-(methylamino)isobutyric acid (MeAIB) or saline, fluciclovine PET tumor-to-background activity was 43.6 (± 5.4)% and 25.3 (± 5.2)% lower in BCH (n = 6) and MeAIB (n = 5) injected PC3 Luc xenografts, respectively, compared to saline-injected controls (n = 2). Partial inhibition of fluciclovine uptake by BCH and MeAIB can be demonstrated in vivo similar to previous in vitro modeling.

Fibrosis in ulcerative colitis is directly linked to severity and chronicity of mucosal inflammation.

BACKGROUND: Fibrosis in ulcerative colitis has remained largely unexplored despite its clinical implications. AIMS: This cross-sectional study was aimed at characterising the presence, anatomical location and degree of ulcerative colitis-associated fibrosis and its possible link to clinical parameters. METHODS: Seven hundred and six individual tissue cross-sections derived every 10 cm along the length of 89 consecutive Ulcerative colitis colectomy specimens were examined and compared to Crohn's disease colitis, diverticular disease and uninvolved areas from colorectal cancer patients. Degree of inflammation, fibrosis and morphometric measurements of all layers of the intestinal wall were evaluated. Three gastrointestinal pathologists independently assessed colon sections stained with haematoxylin and eosin, Masson trichrome and Sirius red. Clinical data were collected prospectively. RESULTS: Submucosal fibrosis was detected in 100% of ulcerative colitis colectomy specimens, but only in areas affected by inflammation. Submucosal fibrosis was associated with the severity of intestinal inflammation (Spearman correlations rho (95% confidence interval): 0.58 (P < 0.001) and histopathological changes of chronic mucosal injury, but not active inflammation. Colectomy for refractory disease rather than presence of dysplasia was associated with increased fibrosis and a thicker muscularis mucosae, whereas a thinner muscularis mucosae was associated with anti-tumour necrosis factor therapy. No feature on endoscopic mucosal biopsies could predict the underlying amount of fibrosis or the thickness of the muscularis mucosae. CONCLUSIONS: A significant degree of fibrosis and muscularis mucosae thickening should be considered as common complications of chronic progressive ulcerative colitis. These features may have clinical consequences such as motility abnormalities and increased wall stiffness.

Clinical studies on progressive retinal atrophy in 31 dogs.

During a 2-year period, 31 cases of a hereditary retinal degeneration in dogs bred in India were found mainly suspected for progressive retinal atrophy (PRA) with typical history of initial nyctalopia followed by hemeralopia. Out of 31 PRA suspected dogs, 8 dogs (26%) were from the age group of 1-5 years, 15 (48%) 6-10 years and the rest (26%) 11-15 years. The most predominant breed was Spitz (18 dogs, 58%). Detailed ophthalmologic examinations included Schirmer's tear test, fluorescein stain, applanation tonometry, slit lamp biomicroscopy and ocular ultrasound in appropriate cases. Ophthalmoscopic and fundoscopic changes included hyperreflectivity and discoloration of the tapetal area, marked attenuation of retinal vessels, depigmentation in non-tapetal area and optic disc atrophy with scalloped borders. Electroretinograms (ERG) recorded in 13 PRA-affected cases revealed non-recordable extinguished (flatline) ERG responses. A reduction mainly of a- and b-wave amplitudes in the ERG indicated a generalized photoreceptor disease.

Quasiballistic quantum transport through Ge/Si core/shell nanowires.

We study signatures of ballistic quantum transport of holes through Ge/Si core/shell nanowires at low temperatures. We observe Fabry-Pérot interference patterns as well as conductance plateaus at integer multiples of 2e 2/h at zero magnetic field. Magnetic field evolution of these plateaus reveals relatively large effective Landé g-factors. Ballistic effects are observed in nanowires with silicon shell thickness of 1-3 nm, but not in bare germanium wires. These findings inform the future development of spin and topological quantum devices which rely on ballistic sub-band-resolved transport.