Nutritional aspects of non-conventional edible plants from Brazil: Caruru (Amaranthus spinosus L) and trapoeraba (Commelina benghalensis).

Caruru (Amaranthus spinosus L) and trapoeraba (Commelina benghalensis) are NCEPs introduced into Brazil and are widely used by certain communities. Given the lack of information on carotenoids, vitamins, and minerals present in A. spinosus and C. benghalensis grown in Brazil, this study aimed to determine the proximate composition and the micronutrient profile of these two NCEPs obtained from family farming in the Middle Doce River (Médio Rio Doce) region in the state of Minas Gerais, Brazil. The proximate composition was evaluated using AOAC methods, vitamin E by HPLC with fluorescence detection, vitamin C and carotenoids by HPLC-DAD, and minerals by atomic emission spectrometry with inductively coupled plasma. In summary, the leaves of A. spinosus exhibited a high content of dietary fiber (10.20 g.100 g-1), potassium (708.8 mg·100 g-1), iron (4.0 mg·100 g-1) and ß-carotene (6.94 mg·100 g-1), while the leaves of C. benghalensis were sources of potassium (1399.31 mg·100 g-1), iron (5.7 mg·100 g-1), calcium (163 mg·100 g-1), zinc (1.3 mg·100 g-1), ascorbic acid (23.61 mg·100 g-1), and ß-carotene (31.33 mg·100 g-1). It was therefore concluded that C. benghalensis and A. spinosus, especially, presented excellent potential as important nutritional sources for human consumption, highlighting the gap existing between the available technical and scientific material, thus making them an important and necessary axis of research.

Proximate composition and characterization of the vitamins and minerals of dandelion (Taraxacum officinale) from the Middle Doce River region - Minas Gerais, Brazil.

This study aimed to determine the chemical composition and the vitamin, carotenoid, and mineral profile in dandelion (Taraxacum officinale) collected from the Middle Doce River region (Médio Rio Doce) in the state of Minas Gerais, Brazil. To accomplish this, the physicochemical parameters, such as titratable acidity, pH, and soluble solids were determined, in addition to the evaluation of the plants' proximate composition (moisture, ash, proteins, dietary fibers, and lipids). The vitamin E, carotenoids and vitamin C were determined by HPLC and the minerals were analysed by inductively coupled plasma atomic emission spectrometry. The T. officinale samples presented a low content of macronutrients, a total energy value of 27.88 kcal.100 g-1 FW, a high fiber content (3.7 g.100 g-1 FW), low levels of total vitamin E (43.67 µg.100 g-1 FW), total carotenoids of 11.95 g.100 g-1 FW, and did not present vitamin C in detectable levels. The mineral analysis revealed a high concentration of iron, manganese, copper, zinc, and selenium, and small amounts or traces of aluminium, cadmium, nickel, and chromium. In conclusion, T. officinale was shown to be an important source of nutrients, especially fiber, iron and manganese.

Chemical Composition and Nutritional Value of Three Sonchus Species.

Species of unconventional food plants of the genus Sonchus are widely consumed in rural populations living in the Brazilian Atlantic Forest. This study investigated the nutritional composition of S. oleraceus, S. asper, and S. arvensis species. The centesimal composition was investigated according to the norms of the Association of Official Analytical Chemists, the occurrence and concentration of carotenoids and vitamins through High-Performance Liquid Chromatography, and minerals with the aid of atomic emission spectrometry in inductively coupled plasma. There was no significant difference between the water content found in the three species. However, S. asper showed higher concentrations of lipids (1.32 g/100 g), carbohydrates (0.34 g/100 g), total carotenoids (5.58 mg/100 g), and Ca (96.25 mg/100 g), while S. arvensis had the highest concentration of vitamins E (72.98 µg/100 g) and K (604.85 mg/100 g). S. oleraceus showed higher concentrations of Fe (23.74 mg/100 g). Statistically, fibers and ash presented the same proportions in S. asper and S. arvensis, as well as proteins in S. oleraceus and S. asper species. The availabilities of these vegetables together with their high nutritional value are important factors that contribute to ensuring food security for families that have these species in their diet.

Chemical composition, vitamins, and minerals of family farming biribiri (Averrhoa bilimbi L) in the middle Doce River region, Minas Gerais, Brazil / Composição química, vitaminas e minerais do biribiri (Averrhoa bilimbi L) da agricultura familiar da região do médio Rio Doce, Minas Gerais, Brasil

This study evaluated physical characteristics, chemical composition, content of vitamin C, vitamin E, carotenoids, and minerals in biribiri fruits (Averrhoa bilimbi) from the Middle Doce River region (Minas Gerais, Brazil). Titratable acidity was determined by volumetric neutralization, pH by direct potentiometry, soluble solids by refractometry, humidity by gravimetry, ash by calcination in muffle, proteins by the micro-Kjeldahl method, dietary fiber by non-enzymatic gravimetric method and lipids using a Soxhlet extractor. Carotenoids and vitamin C were analyzed by high-performance liquid chromatography (HPLC) and vitamin E by HPLC with fluorescence detector. Fourteen minerals were analyzed by inductively coupled plasma atomic emission spectrometry. Biribiri showed high yield of edible portion (100%), low lipid, protein and carbohydrate content, and; consequently, low total energy value (25.36 kcal 100 g-¹). The fruit also showed low dietary fiber content (0.62 g 100 g-¹), total vitamin E (17.62 µg 100 g-¹), total carotenoids (0.32 g 100 g-¹), and high vitamin C, zinc, copper, iron content, manganese, molybdenum and chrome content. Regarding the heavy metals, the fruit showed no cadmium, and traces of aluminum and nickel. In conclusion, biribiri presented low energy value and expressive contents of dietary fibers, vitamin C, iron, manganese, molybdenum, chromium, zinc, and copper.

Este estudo teve como objetivo avaliar as características físicas, a composição química, teor de vitamina C, vitamina E, carotenoides e minerais em frutos de biribiri (Averrhoa bilimbi) do território do Médio Rio Doce (Minas Gerais, Brasil). A acidez titulável foi determinada por neutralização volumétrica, o pH por potenciometria direta, os sólidos solúveis por refratometria, a umidade por gravimetria, as cinzas por calcinação em mufla, as proteínas pelo método micro-Kjeldahl, as fibras alimentares pelo método gravimétrico não enzimático e os lipídios usando um extrator Soxhlet. Os carotenoides e a vitamina C foram analisados por cromatografia líquida de alta eficiência (CLAE). A vitamina E foi analisada por CLAE com detector de fluorescência e quatorze minerais foram analisados por espectrometria de emissão atômica com plasma indutivamente acoplado. O biribiri apresentou alto rendimento de porção comestível (100%), baixos teores de lipídios, proteínas e carboidratos e, consequentemente, baixo valor total de energia (25,36 kcal 100 g-¹). Os frutos também apresentaram baixos teores de fibra alimentar (0,62 g 100 g-¹), vitamina E total (17,62 µg 100 g-¹), carotenoides totais (0,32 g 100 g-¹) e altos teores de vitamina C, zinco, cobre, ferro, manganês, molibdênio e conteúdo cromado. Em relação aos metais pesados, os frutos não apresentaram cádmio e tiveram vestígios de alumínio e níquel. Em conclusão, o biribiri apresentou baixo valor energético e conteúdos expressivos de fibras alimentares, vitamina C, ferro, manganês, molibdênio, cromo, zinco e cobre.

Comparison of bioactive compounds and nutrient contents in whey protein concentrate admixture of turmeric extract produced by spray drying and foam mat drying.

We developed a whey protein admixture of turmeric extract by spray drying (TWPC-SD) and by foam mat drying (TWPC-FMD) and compared its bioactive compounds and nutrients contents. TWPC samples were evaluated for preference and acceptability. Vitamins and carotenoids were determined by high-performance liquid chromatography. Total phenolics, curcumin and antioxidant capacity were determined by spectrophotometry. Centesimal composition was performed according to the Association of Official Analytical Chemists. Chemical elements were determined by inductively coupled plasma optical emission spectrometry. TWPC containing 3.6 mg of curcumin showed good acceptability. Carotenoids and riboflavin were not detected in either TWPC. Vitamin C content was maintained, and antioxidant capacity was increased in both products (p < 0.05). TWPC-SD showed higher total phenolic and curcumin contents compared to TWPC-FMD (p < 0.05). Thus, the TWPC-SD is a good alternative for human consumption since it showed good sensory acceptability and its nutrients and bioactive compounds can contribute to human health.

Stability of B vitamins, vitamin E, xanthophylls and flavonoids during germination and maceration of sorghum (Sorghum bicolor L.).

The impact of maceration and germination on the concentration of bioactive compounds still needs to be evaluated. The stability of B complex vitamins (thiamine, riboflavin, pyridoxine), vitamin E (α, ß, γ, δ tocopherols and tocotrienols), xanthophylls (lutein and zeaxanthin) and flavonoids (3-deoxyanthocyanidins-3-DXAs, flavones and flavanones) was evaluated in sorghum grains subjected to maceration and germination, using High Performance Liquid Chromatography. Maceration and germination reduced thiamine and pyridoxine concentrations (retentions ranging from 3.8 to 50.2%). Riboflavin and Vitamin E concentrations were not affected by maceration. Germination increased riboflavin and reduced vitamin E. 3-DXAs were sensitive to maceration and germination (retentions of 69.6% and 69.9%, respectively). Flavones contents decreased with germination. Our results indicate that, after germination and/or maceration, sorghum had important nutritional and functional value. Thus, its intake, mainly in macerated forms, should be encouraged, since concentrations of riboflavin, vitamin E and flavones were not altered during this processing.

Nutritional value of non-conventional vegetables prepared by family farmers in rural communities / Valor nutricional de hortaliças não convencionais preparadas por agricultores familiares em comunidades rurais

Four most consumed non-conventional vegetables were analyzed raw and after cooking techniques routinely used by family farmers: ora-pro-nobis (Pereskia aculeata Mill.); wild mustard (Sinapis arvensis L.), serralha (Sonchus arvensis L), and capiçova (Erechtites valeriana). Chemical composition was determined according to AOAC. Vitamin C, vitamin E and carotenoids were determined by high-performance liquid chromatography, and phenolic compounds and minerals by spectrophotometry. Vitamin E and carotenoids concentrations were higher in stir fried wild mustard (7.68 mg.100 g-1 and 7.45 mg.100 g-1, respectively). Cooking reduced some minerals concentration in the non-conventional vegetables, but increased vitamins and carotenoids concentrations. The vegetables presented high content of minerals but low protein concentration and total energy content. Non-conventional vegetables can be considered of excellent nutritional value and frequent consumption of these vegetables can contribute to improve the feeding of farmers and their families.(AU)

Quatro hortaliças não convencionais mais consumidas foram analisadas cruas e após as técnicas de cocção utilizadas rotineiramente pelos agricultores familiares: ora-pro-nobis (Pereskia aculeata Mill.); mostarda selvagem (Sinapis arvensis L.), serralha (Sonchus arvensis L) e capiçova (Erechtites valeriana). A composição química foi determinada de acordo com a AOAC. A vitamina C, vitamina E e os carotenoides foram determinados por cromatografia líquida de alta eficiência (CLAE), e compostos fenólicos e minerais foram determinados por espectrofotometria. As concentrações de vitamina E e carotenoides foram maiores na mostarda selvagem (7,68 mg.100 g-1e 7,45 mg.100 g-1, respectivamente). A cocção reduziu a concentração de alguns minerais nas hortaliças não convencionais, mas aumentou as concentrações de vitaminas e carotenoides. As hortaliças apresentaram alto teor de minerais, mas baixa concentração protéica e valor energético total. As hortaliças não convencionais podem ser consideradas de excelente valor nutricional. O consumo frequente dessas hortaliças pode contribuir para melhorar a alimentação dos agricultores e suas famílias.(AU)

Quali-Quantitative Profile of Native Carotenoids in Kumquat from Brazil by HPLC-DAD-APCI/MS.

In this study the native carotenoids composition in kumquat (Fortunella margarita) (peel + pulp) from Brazil was determined for the first time by a HPLC-DAD-APCI/MS (high performance liquid chromatography-diode array detector-atmospheric pressure chemical ionization/mass spectrometry), methodology. Eleven carotenoids were successfully identified and quantified in kumquat: four carotenoids in the free form and seven carotenoids in the esterified form. ß-citraurin-laurate was the carotenoid found in the highest content (607.33 µg/100 g fresh matter), followed by ß-cryptoxanthin-laurate (552.59 µg/100 g). The different esterified forms of ß-citraurin and ß-cryptoxanthin represented 84.34% of the carotenoids found, which demonstrates the importance of esterification in natural fruits. ß-carotene and free xanthophylls (ß-cryptoxanthin, lutein and zeaxanthin) represented 5.50% and 14.96%, respectively, of total carotenoids in kumquat. The total carotenoid content of kumquat from Brazil was very high (2185.16 µg/100 g), suggesting that this fruit could contribute significantly to the intake of important bioactive compounds by the population.

Nutritional value of non-conventional vegetables prepared by family farmers in rural communities / Valor nutricional de hortaliças não convencionais preparadas por agricultores familiares em comunidades rurais

ABSTRACT: Four most consumed non-conventional vegetables were analyzed raw and after cooking techniques routinely used by family farmers: ora-pro-nobis (Pereskia aculeata Mill.); wild mustard (Sinapis arvensis L.), serralha (Sonchus arvensis L), and capiçova (Erechtites valeriana). Chemical composition was determined according to AOAC. Vitamin C, vitamin E and carotenoids were determined by high-performance liquid chromatography, and phenolic compounds and minerals by spectrophotometry. Vitamin E and carotenoids concentrations were higher in stir fried wild mustard (7.68 mg.100 g-1 and 7.45 mg.100 g-1, respectively). Cooking reduced some minerals concentration in the non-conventional vegetables, but increased vitamins and carotenoids concentrations. The vegetables presented high content of minerals but low protein concentration and total energy content. Non-conventional vegetables can be considered of excellent nutritional value and frequent consumption of these vegetables can contribute to improve the feeding of farmers and their families.

RESUMO: Quatro hortaliças não convencionais mais consumidas foram analisadas cruas e após as técnicas de cocção utilizadas rotineiramente pelos agricultores familiares: ora-pro-nobis (Pereskia aculeata Mill.); mostarda selvagem (Sinapis arvensis L.), serralha (Sonchus arvensis L) e capiçova (Erechtites valeriana). A composição química foi determinada de acordo com a AOAC. A vitamina C, vitamina E e os carotenoides foram determinados por cromatografia líquida de alta eficiência (CLAE), e compostos fenólicos e minerais foram determinados por espectrofotometria. As concentrações de vitamina E e carotenoides foram maiores na mostarda selvagem (7,68 mg.100 g-1e 7,45 mg.100 g-1, respectivamente). A cocção reduziu a concentração de alguns minerais nas hortaliças não convencionais, mas aumentou as concentrações de vitaminas e carotenoides. As hortaliças apresentaram alto teor de minerais, mas baixa concentração protéica e valor energético total. As hortaliças não convencionais podem ser consideradas de excelente valor nutricional. O consumo frequente dessas hortaliças pode contribuir para melhorar a alimentação dos agricultores e suas famílias.

Consumption of a drink containing extruded sorghum reduces glycaemic response of the subsequent meal.

PURPOSE: Glycaemic control is essential to prevent the manifestation of diabetes in predisposed individuals and the development of associated comorbidities. It is believed that sorghum may modulate the glucose response. In this study, we investigated the effect of extruded sorghum consumption, and the profile of bioactive compounds, on postprandial glycaemia of a subsequent meal in normal weight and normoglycaemic subjects. METHODS: This was a randomized, single-blind, crossover designed study. After a 12 h overnight fasting, ten subjects reported to the laboratory to participate in four experimental sessions, and consumed one of three sorghum test drinks: sorghum P 3-DXAs (with proanthocyanidins-P and rich in 3-deoxyanthocyanidins-3-DXAs); 3-DXAs (without proanthocyanidins and rich in 3-DXAs); and control (low in 3-DXAs and without proanthocyanidins); or a non-sorghum drink. 30 min later, the subjects consumed a glucose solution (25 g glucose). Glycaemic response was monitored at times 0 (before glucose solution), 15, 30, 45, 60, 90, 120 min (after glucose solution consumption). The incremental areas under the glycaemic curve (iAUC) were calculated by the trapezoidal method. RESULTS: Intake of P 3-DXAs drink before the glucose solution resulted in a postprandial iAUC lower than the other sorghum test drinks. Sorghum drinks minimized the postprandial glycaemia peak. CONCLUSION: Sorghum drinks consumption, especially the P 3-DXAs drink, 30 min before the glucose solution resulted in lower iAUC compared to the non-sorghum drink, leading to a lower glycaemic response.

Chemical composition of Brazilian chia seeds grown in different places.

This study investigated and compared the occurrence and concentration of macronutrients, moisture, ash, dietary fiber, fatty acids, minerals, carotenoids, vitamins, flavonoids, phenolic compounds, antioxidant activity, phytate and tannin in Brazilian chia seeds grown in the states of Mato Grosso (MT) and Rio Grande do Sul (RS). High concentrations of lipids (31.2g.100g-1, on average), proteins (18.9g.100g-1, on average), dietary fiber (35.3g.100g-1, on average), vitamin E (8,203.6µg.100g-1, on average) were observed. Similar values for total phenolic compounds and phytic acid in chia seeds from both regions were observed. Chia grown in RS showed higher antioxidant activity than chia grown in MT, and the tannin concentrations were higher in chia seeds grown in Mato Grosso (19.08±1.08eq.catequina/gsample). In conclusion, Brazilian chia seeds showed high concentrations of lipids, proteins, total dietary fiber, minerals and vitamin E.

Sorghum (Sorghum bicolor L.): Nutrients, bioactive compounds, and potential impact on human health.

Sorghum is the fifth most produced cereal in the world and is a source of nutrients and bioactive compounds for the human diet. We summarize the recent findings concerning the nutrients and bioactive compounds of sorghum and its potential impact on human health, analyzing the limitations and positive points of the studies and proposing directions for future research. Sorghum is basically composed of starch, which is more slowly digested than that of other cereals, has low digestibility proteins and unsaturated lipids, and is a source of some minerals and vitamins. Furthermore, most sorghum varieties are rich in phenolic compounds, especially 3-deoxyanthocyanidins and tannins. The results obtained in vitro and in animals have shown that phenolics compounds and fat soluble compounds (polycosanols) isolated from sorghum benefit the gut microbiota and parameters related to obesity, oxidative stress, inflammation, diabetes, dyslipidemia, cancer, and hypertension. The effects of whole sorghum and its fractions on human health need to be evaluated. In conclusion, sorghum is a source of nutrients and bioactive compounds, especially 3-deoxyanthocyanidins, tannins, and polycosanols, which beneficially modulate, in vitro and in animals, parameters related to noncommunicable diseases. Studies should be conducted to evaluate the effects of different processing on protein and starch digestibility of sorghum as well as on the profile and bioavailability of its bioactive compounds, especially 3-deoxyanthocyanidins and tannins. Furthermore, the benefits resulting from the interaction of bioactive compounds in sorghum and human microbiota should be studied.

Quinoa: Nutritional, functional, and antinutritional aspects.

We have prepared a review of the physical-chemical composition and the functional and anti-nutritional properties of quinoa (Chenopodium quinoa Willd.). It is a plant of the Chenopodiaceae family, originally from the Andean regions, adaptable to different types of soils and climatic conditions. Its composition has attracted the attention of scientific community for its high nutritional value, being rich in proteins, lipids, fibers, vitamins, and minerals, with an extraordinary balance of essential amino acids. It is also gluten-free, a characteristic that enables its use by celiac patients. In spite of all these attributes, quinoa is not widely used by consumers due to the high cost of imported grain and little knowledge of its benefits. More studies are required to increase knowledge about this "pseudo-cereal" to demonstrate its functional and nutritional benefits and to study its anti-nutritional effects, since it presents high commercial value and excellent nutritional quality.

Effect of cooking methods on the stability of thiamin and folic acid in fortified rice.

Rice fortification offers great potential to deliver essential micronutrients to a large part of the world population. However, high temperatures required for cooking rice are deleterious to thermally labile micronutrients. This study evaluated the content and stability of thiamin and folic acid in fortified rice after different cooking methods (stir-frying, boiling, cooking in a microwave oven and boiling in a Food Service). The analyses were performed by High Performance Liquid Chromatography (HPLC). The fortified rice showed the highest thiamin content (0.97 mg/kg) and retention (65.4%) when cooked in microwave oven, and for folic acid the lowest content (0.17 mg/kg) and the highest retention (96.11%) when cooked in a Food Service and stir-fried, respectively. In conclusion, the stability of thiamin and folic acid varied according to the cooking method and the stability of folic acid presented higher percentages in relation to thiamin in the different methods. [Formula: see text].

Diet Quality and Adequacy of Nutrients in Preschool Children: Should Rice Fortified with Micronutrients Be Included in School Meals?

Feeding is indicative of the nutritional status of children, however micronutrient deficiency is common in this age group. We evaluated the impact of inclusion of rice (Ultra Rice(®) (UR(®))) fortified with iron, zinc, thiamin and folic acid on laboratory measurements and the nutrient intake of children. Ninety-nine preschoolers (2-6 years; 42.6% male) from two preschools participated, one of which received UR(®) added to polished rice as part of school meals (test preschool) and the other received only polished rice (control preschool). Biochemical evaluations were performed before and after four months of intervention. Feeding was assessed by direct weighing of food, complemented by 24-h recalls, and the diet was assessed by the Healthy Eating Index (HEI) adapted to the Brazilian reality. The fortified rice improved the levels of zinc (p < 0.001), thiamine (p < 0.001), folic acid (p = 0.003), mean corpuscular hemoglobin (p < 0.001) and mean corpuscular hemoglobin concentration (p < 0.001). The inadequacy percentages of thiamine, folic acid and iron were lower among preschoolers from the test preschool. This study demonstrated the effectiveness of using UR(®) on laboratory measurements of children. The inadequate intake of thiamine, folic acid and iron was also reduced, making the fortified rice an interesting strategy in school feeding programs.

Avaliação da estabilidade da capacidade antioxidante e de parâmetros físico-químicos de néctares de frutas caseiros / Assessment of the antioxidant capacity and of the physicalchemicalsparameters stability in homemade fruit nectars

Este estudo avaliou a capacidade antioxidante e os indicadores físico-químicos de néctares caseirosde laranja, manga e maracujá, mantidos sob refrigeração (5 ± 2 oC) por 24 horas. Os néctaresforam preparados em laboratório e mantidos sob refrigeração, simulando as condições domésticas.As análises foram realizadas após o preparo (T0) e durante o acondicionamento sob refrigeração(1 h, 4 h e 24 h). Os sólidos solúveis, pH e cor foram determinados respectivamente por refratometria,potenciometria e colorimetria. Carotenoides e ácido ascórbico foram analisados por cromatografialíquida de alta eficiência; a concentração de compostos fenólicos foi determinada utilizando-seo reagente de Folin Ciocalteau e a atividade antioxidante pelo teste do DPPH. Ácido ascórbico,β-caroteno e compostos fenólicos foram identificados em todos os néctares. Foram encontradosα-caroteno e β-criptoxantina no néctar de laranja e licopeno no néctar de manga. Durante 24 horasde refrigeração, os compostos analisados e a atividade antioxidante mantiveram-se estáveis. De formageral, os parâmetros físico-químicos também se mantiveram estáveis durante o período avaliado.Em conclusão, sob as condições utilizadas no presente estudo, os néctares não apresentaramalteração da capacidade antioxidante, podendo ser considerados fontes de carotenoides e vitamina C,mesmo se consumidos após 24 horas de preparo.

The objective of the study was to evaluate the antioxidant capacity and the physical-chemicalindicators of homemade nectars of orange, mango and passion fruit, kept under refrigeration(5 ± 2 °C) for 24 hours. The nectars were prepared in laboratory and kept under refrigerationsimulating the domestic conditions. The samples analyses were performed after their preparations(T0) and during the refrigerated storage (1 h, 4 h and 24 h). Soluble solids, pH and color weredetermined by refractometry, colorimetry and potentiometry, respectively. Carotenoids andascorbic acid were analyzed by high performance liquid chromatography, the concentrationof phenolic compounds were determined by using Folin Ciocalteau reagent, and the antioxidantactivity by the DPPH test. Ascorbic acid, β-carotene and phenolic compounds were identified inall of the analyzed nectars samples. The α-carotene and β-cryptoxanthin were found in orangenectar and the lycopene in mango nectar samples. During the refrigeration for 24 hours,the analyzed compounds and the antioxidant activity remained stable. In general, thephysical-chemicals parameters also remained stable during the storage for 24 hours. In conclusion,under the conditions used in this study, the nectars might be considered as antioxidant sources,even if consumed after being prepared 24 hours before.

Avaliação da estabilidade da capacidade antioxidante e de parâmetros físico-químicos de néctares de frutas caseiros / Assessment of the antioxidant capacity and of the physicalchemicals parameters stability in homemade fruit nectars

Este estudo avaliou a capacidade antioxidante e os indicadores físico-químicos de néctares caseiros de laranja, manga e maracujá, mantidos sob refrigeração (5 ± 2 o C) por 24 horas. Os néctares foram preparados em laboratório e mantidos sob refrigeração, simulando as condições domésticas. As análises foram realizadas após o preparo (T0) e durante o acondicionamento sob refrigeração (1 h, 4 h e 24 h). Os sólidos solúveis, pH e cor foram determinados respectivamente por refratometria, potenciometria e colorimetria. Carotenoides e ácido ascórbico foram analisados por cromatografia líquida de alta eficiência; a concentração de compostos fenólicos foi determinada utilizando-se o reagente de Folin Ciocalteau e a atividade antioxidante pelo teste do DPPH. Ácido ascórbico, β-caroteno e compostos fenólicos foram identificados em todos os néctares. Foram encontrados α-caroteno e β-criptoxantina no néctar de laranja e licopeno no néctar de manga. Durante 24 horas de refrigeração, os compostos analisados e a atividade antioxidante mantiveram-se estáveis. De forma geral, os parâmetros físico-químicos também se mantiveram estáveis durante o período avaliado. Em conclusão, sob as condições utilizadas no presente estudo, os néctares não apresentaram alteração da capacidade antioxidante, podendo ser considerados fontes de carotenoides e vitamina C, mesmo se consumidos após 24 horas de preparo.

The objective of the study was to evaluate the antioxidant capacity and the physical-chemical indicators of homemade nectars of orange, mango and passion fruit, kept under refrigeration (5 ± 2 °C) for 24 hours. The nectars were prepared in laboratory and kept under refrigeration simulating the domestic conditions. The samples analyses were performed after their preparations (T0) and during the refrigerated storage (1 h, 4 h and 24 h). Soluble solids, pH and color were determined by refractometry, colorimetry and potentiometry, respectively. Carotenoids and ascorbic acid were analyzed by high performance liquid chromatography, the concentration of phenolic compounds were determined by using Folin Ciocalteau reagent, and the antioxidant activity by the DPPH test. Ascorbic acid, β-carotene and phenolic compounds were identified in all of the analyzed nectars samples. The α-carotene and β-cryptoxanthin were found in orange nectar and the lycopene in mango nectar samples. During the refrigeration for 24 hours, the analyzed compounds and the antioxidant activity remained stable. In general, the physical-chemicals parameters also remained stable during the storage for 24 hours. In conclusion, under the conditions used in this study, the nectars might be considered as antioxidant sources, even if consumed after being prepared 24 hours before.

Estado nutricional relativo ao ferro, zinco e vitamina A de pré-escolares inseridos em um programa de educação alimentar e nutricional / Nutritional status relative to iron, zinc and vitamin A to preschool children enrolled in a program of food and nutrition education

O objetivo deste trabalho foi avaliar o estado nutricional de ferro, zinco e vitamina A de pré-escolares inseridos em um programa de educação alimentar e nutricional. Foi realizado um programa de educação alimentar e nutricional que consistiu de 6 intervenções com 54 crianças de 2 a 6 anos de idade, pais e funcionários de creches de tempo integral. Parâmetros bioquímicos (hemoglobina, ferritina, ferro sérico, zinco plasmático e eritrocitário e retinol plasmático), antropométricos (E/I, P/I, P/E e IMC/I) e dietéticos foram avaliados após 6 meses de intervenção. A maioria das crianças apresentou adequação nos índices E/I, P/I e P/E (99%, 92% e 66%, respectivamente). Segundo o IMC/I, 73% estavam eutróficos, 16% apresentaram risco de sobrepeso, 5% sobrepeso e 6% obesos. A prevalência da deficiência de anemia reduziu de 25% para 11%, a adequação do zinco plasmático aumentou de 16% para 56%, enquanto os níveis marginais de vitamina A aumentaram de 5% para 39%. Não houve mudanças nos parâmetros antropométricos e observou-se maior frequência de consumo de alimentos fontes de ferro, zinco e vitamina A. As ações de educação nutricional contribuíram para a redução da deficiência de minerais, coexistente com o excesso de peso. Intervenções em longo prazo são necessárias para a formação de hábitos alimentares saudáveis, controle do peso e redução de carências de micronutrientes.

Rice and Bean Targets for Biofortification Combined with High Carotenoid Content Crops Regulate Transcriptional Mechanisms Increasing Iron Bioavailability.

Iron deficiency affects thousands of people worldwide. Biofortification of staple food crops aims to support the reduction of this deficiency. This study evaluates the effect of combinations of common beans and rice, targets for biofortification, with high carotenoid content crops on the iron bioavailability, protein gene expression, and antioxidant effect. Iron bioavailability was measured by the depletion/repletion method. Seven groups were tested (n = 7): Pontal bean (PB); rice + Pontal bean (R + BP); Pontal bean + sweet potato (PB + SP); Pontal bean + pumpkin (PB + P); Pontal bean + rice + sweet potato (PB + R + P); Pontal bean + rice + sweet potato (PB + R + SP); positive control (Ferrous Sulfate). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency (HRE), gene expression of divalente metal transporter 1 (DMT-1), duodenal citocromo B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The test groups, except the PB, showed higher HRE (p < 0.05) than the control. Gene expression of DMT-1, DcytB and ferroportin increased (p < 0.05) in the groups fed with high content carotenoid crops (sweet potato or pumpkin). The PB group presented lower (p < 0.05) TAC than the other groups. The combination of rice and common beans, and those with high carotenoid content crops increased protein gene expression, increasing the iron bioavailability and antioxidant capacity.

B vitamins related to homocysteine metabolism in adults celiac disease patients: a cross-sectional study.

BACKGROUND: The only treatment for celiac disease is the gluten-free diet. Few studies have assessed the nutritional adequacy of this diet, especially of B vitamins related to homocysteine metabolism. The aim of this study was to assess the nutritional status and serum concentrations of B vitamins involved in homocysteine metabolism, and to determine whether the dietary intake of these vitamins are meeting Dietary Reference Intakes in celiac patients. METHODS: A cross-sectional study enrolled a total of 20 celiac patients (36.3 ± 13.7 years old; 65% women), following strict gluten-free diet (GFD) and 39 healthy controls matched by sex and age. The dietary intake was assessed by 3-day food records, and serum concentrations of homocysteine and vitamins B6, B12, and folate were determined after overnight fasting. Comparisons between the two groups were performed by Student's t test or Mann-Whitney U-test, for continuous variables. Pearson's chi-square test or Fisher's exact test was used for categorical variables. An alpha level of 5% were considered significant. RESULTS: Celiac patients had lower serum folate concentrations (7.7 ± 3.5 ng/mL, P < 0.05) than controls. All celiac patients had folate intake below the Estimated Average Requirement (EAR) (130.8 ± 53.6 µg/d). However, only a small proportion of celiac patients had hyperhomocysteinemia. CONCLUSIONS: Celiac patients treated with GFD presented inadequacy of dietary folate intake and low-serum concentrations of folate, suggesting that more attention should be given to the quality of the nutrients offered by the GFD, as it constitutes a lifelong treatment.