Surgical Correction of Ascending Aortic Aneurysm Without Coronary Dilatation After Kawasaki Disease in a 3-Year-Old Child

Abstract Kawasaki disease (KD) is an inflammatory condition that may affect genetically predisposed individuals in pediatric ages after infectious/environmental triggering. An infrequent finding associated with KD is ascending aortic aneurysm during or after the acute phase of the disease. In this Multimedia presentation, we describe a three-year-old girl submitted to surgical treatment.

Surgical Correction of Ascending Aortic Aneurysm Without Coronary Dilatation After Kawasaki Disease in a 3-Year-Old Child.

Kawasaki disease (KD) is an inflammatory condition that may affect genetically predisposed individuals in pediatric ages after infectious/environmental triggering. An infrequent finding associated with KD is ascending aortic aneurysm during or after the acute phase of the disease. In this Multimedia presentation, we describe a three-year-old girl submitted to surgical treatment.

A longitudinal survey of gastrointestinal parasites of the black-eared opossum Didelphis aurita at an urban-sylvatic interface area in Southeast Brazil: a morphological and ecological characterization based on helminth eggs.

Parasites are important organisms in ecosystem dynamics and take part in the regulation and structure of host populations. The prevalence, abundance, and distribution of parasites in their host species may be affected by biotic and abiotic factors. Most studies of helminths of Brazilian marsupials are taxonomic descriptions or re-descriptions and records of occurrence. The use of noninvasive techniques for studying parasitic worms of vertebrate hosts is more common in large or threatened species. The aims of this study were to describe and identify the helminth morphotypes and to analyse the parasitological parameters of gastrointestinal helminth eggs obtained in faecal samples of marsupial Didelphis aurita at the Fiocruz Atlantic Forest Biological Station and surroundings, municipality of Rio de Janeiro, southeast Brazil. The common opossums were sampled during a capture-mark-recapture study. Faecal samples collected from each animal were analysed for helminth egg diagnosis using the methods of flotation in sugar solution and sedimentation. Eggs were compared with samples obtained from the uterus of adult worms obtained from a previous study carried out in the same area. Eleven helminth egg morphotypes were found. Among them, seven were identified at the species level. The highest values of parasitic load and prevalence were observed for the families Viannaiidae and Trichuridae. A significant relationship was found between the number of morphotypes and host body size in females. The influence of parasite load on host condition factor or body size was not observed.

Fiabilidad y validez factorial del instrumento para medir calidad de vida en el trabajo "CVT-Gohisalo" (versión breve) / Reliability and the Factorial Validity of the Instrument for Measuring Quality of Work Life "CVT-GOHISALO" (brief)

Resumen Objetivo: Validar el cuestionario CVT-Gohisalo versión breve para su uso en trabajadores ecuatorianos. Diseño: Análisis factorial exploratorio del cuestionario CVT-Gohisalo con una población de trabajadores ecuatorianos. Métodos: La población se constituyó con 400 sujetos que laboran en diversos frentes laborales de la economía formal en Ecuador. El análisis estadístico se basó en la determinación del coeficiente alfa de Cronbach, porcentaje de varianza explicado y los valores Eigen de cada ítem de la escala. Resultados: El coeficiente α de Cronbach del cuestionario en general fue de 0,911, de manera individual la mayoría de los 7 factores del cuestionario obtuvieron valores satisfactorios; los que presentaron alfa de Cronbach por debajo de 0,7 fueron: integración al puesto de trabajo, que califica como adecuada con déficits (α = 0,6), e igualmente seguridad en el trabajo, cuya calificación se dio como inadecuada (α = 0,5). Conclusiones: Es posible utilizar la versión breve del Cuestionario CVT-Gohisalo con fines de investigación y de evaluación en las empresas en Ecuador.

Abstract Objective: To validate the questionnaire CVT-GOHISALO - short version for use in Ecuadorian workers. Design: Exploratory factor analysis of the questionnaire CVT-GOHISALO with a population of Ecuadorian workers. Methods: The population was composed of 400 subjects who work in various labor shifts in the formal economy in Ecuador. The statistical analysis was based on the determination of the Cronbach's alpha coefficient, proportion of variance explained and eigen values of each item of the scale. Results: The Cronbach's α coefficient of the questionnaire in general was of 0.911. Individually, most of the 7 factors of the questionnaire obtained satisfactory values; the ones that present a Cronbach's alpha coefficient below 0.7 were, "Integration to the job", which qualifies as appropriate with deficits (α =0.6) and "Job reliability", which qualifies as inadequate (α =0.5). Conclusions: It is possible to use the questionnaire CVT-GOHISALO - short version for the purposes of research and evaluation in firms in Ecuador.

Composição e estrutura das comunidades de helmintos de roedores sigmodontíneos no Parque Estadual da Serra do Tabuleiro, Mata Atlântica do estado de Santa Catarina, Brasil / Composição e estrutura das comunidades de helmintos de roedores sigmodontíneos no Parque Estadual da Serra do Tabuleiro, Mata Atlântica do estado de Santa Catarina, Brasil

Apesar da ampla ocorrência de endoparasitos em animais silvestres, ainda há uma carência de informações sobre a helmintofauna destes animais em condições naturais, principalmente de estudos que abordam aspectos ecológicos. O objetivo geral deste trabalho foi descrever a composição de espécies e a estrutura das comunidades de helmintos dos roedores sigmodontíneos Oligoryzomys nigripes, Akodon montensis e Euryoryzomys russatus nas escalas de infracomunidade e comunidades componentes no Parque Estadual da Serra do Tabuleiro, Município de Santo Amaro da Imperatriz, Estado de Santa Catarina, Brasil. Este estudo é o primeiro relato de helmintofauna de roedores em nível específico para esta região, onde foi encontrada e descrita uma nova espécie do gênero Stilestrongylus. Além disso, é o primeiro estudo a analisar a estrutura da metacomunidade de helmintos de A. montensis. Os helmintos foram coletados nas vísceras e nas cavidades torácica e abdominal. Todos os helmintos foram contados e identificados. Em O. nigripes foram encontradas as espécies Guerrerostrongylus zetta e Stilestrongylus lanfrediae (Nematoda, Heligmonellidae)

A helmintofauna de A. montensis incluiu as espécies G. zetta, Trichofreitasia lenti (Nematoda, Heligmonellidae), Trichuris navonae (Nematoda, Trichuridae), Angiostrongylus sp. (Nematoda, Angiostrongylidae), Litomosoides chagasfilhoi (Nematoda, Onchocercidae) e Rodentolepis akodontis (Plathyhelmintes, Cestoda). No roedor E. russatus, três espécies foram encontradas, G. zetta, uma espécie nova denominada de Stilestrongylus rolandoi (Nematoda, Heligmonellidae) e Raillietina guaricanae (Plathyhelmintes, Cestoda). Stilestrongylus rolandoi é distinto de outras espécies do gênero Stilestrongylus pelo seu raio 6 ser curto em relação aos raios 4 e 5, que são longos e robustos, e pelo padrão de bolsa caudal do tipo 1-4 em ambos os lobos. Esta nova espécie tem 27 cristas no meio do corpo em machos e 24 nas fêmeas, e tem uma das maiores razões comprimento do espículo / comprimento do corpo (21-33%) deste gênero. A. montensis apresentou maior riqueza total de helmintos, porém, a riqueza média foi maior em O. nigripes. A espécie de helminto mais abundante e única espécie comum entre os hospedeiros foi G. zetta, que apresentou maiores valores de abundância e prevalência em O. nigripes e maior valor de intensidade em E. russatus. A estrutura da metacomunidade de helmintos de A. montensis na escala de infracomunidade apresentou um padrão aleatório de distribuição das espécies, sendo caracterizado por uma estrutura não coerente ao longo do gradiente ambiental. (AU)

Protein extraction and gel-based separation methods to analyze responses to pathogens in carnation (Dianthus caryophyllus L).

We are currently using a 2-DE-based proteomics approach to study plant responses to pathogenic fungi by using the carnation (Dianthus caryophyllus L)-Fusarium oxysporum f. sp. dianthi pathosystem. It is clear that the protocols for the first stages of a standard proteomics workflow must be optimized to each biological system and objectives of the research. The optimization procedure for the extraction and separation of proteins by 1-DE and 2-DE in the indicated system is reported. This strategy can be extrapolated to other plant-pathogen interaction systems in order to perform an evaluation of the changes in the host protein profile caused by the pathogen and to identify proteins which, at early stages, are involved or implicated in the plant defense response.