[Adoptive transfer of immunity against Nippostrongylus brasiliensis in mice. In vitro restimulation of immune cells before their transfer]. / Transfert adoptif de l'immunité contre Nippostrongylus brasiliensis chez la souris. Effet d'une restimulation in vitro des cellules immunes avant leur transfert.

When mesenteric lymph node cells from infected mice were stimulated during an in vitro culture with exoantigens or with a purified protective antigen of Nippostrongylus brasiliensis, a drop was noted in the number of cells required to transfer protection to new mice. A maximal effect was already obtained after 4 hrs. of culture, but irradiated cells or cells from another mouse strain were unable to mediate this transfer. T cells were more effective than B cells in transferring the protection.

Partial purification of protective antigens from Nippostrongylus brasiliensis in mice.

The purification of antigens from Nippostrongylus brasiliensis, through their ability to provoke cellular proliferation of immune cells and through their recognition by antibodies, led to an antigenic preparation which was extracted from adult worms and which contained only two proteins (MW 14 and 43 Kd). Mice which were vaccinated by the oral route after the entrapment of these two proteins in liposomes were strongly protected.

Vaccination of mice with liposome-entrapped adult antigens of Nippostrongylus brasiliensis.

An immunization procedure was developed to induce protection of mice against the gastrointestinal helminth Nippostrongylus brasiliensis. Mice immunized by the oral route with antigens which were released by adult worms during their in vitro survival in a detergent-containing medium and which were entrapped in liposomes were protected against a challenge infection.

Antigenic analyses of the adult worms of Nippostrongylus brasiliensis by crossed immunoelectrophoresis.

In order to identify and to characterize the antigens from Nippostrongylus brasiliensis which are involved in the in vitro blastogenesis of T cells from infected animals, we have established by crossed immunoelectrophoresis a two dimensional antigenic map of the adult worm homogenate. Polyclonal precipitating rabbit antiserum recognized 26 antigens including five enzymes in these homogenates. The antigenic enzymes, the precipitates of which are only stained by specific staining, are: aldolase, malic enzyme, acid phosphatase, peroxydase and cholinesterase. This antigenic map enable us to identify 26 antigens in adult worm homogenates and to compare them with those which will be contained in purification fractions from these homogenates or in homogenates from other parasites.