Service Dogs and Persons with Disabilities: When COVID-19 Lockdown Changes Their Relationship.

Persons with disabilities, who own service dogs, develop strong relationships with them. Since the COVID-19 pandemic decreased the possibility of social contact and modified human relationships, we hypothesized that the COVID-19 lockdown would influence people with disabilities-service dog relationships. An online survey was conducted during the first COVID-19 lockdown in France and included information (e.g., MONASH score) both in the general context prior to and during the COVID-19 lockdown. Seventy owners participated. Compared to the general context, scores for the Perceived Emotional Closeness and Perceived Costs subscales were significantly higher during the COVID-19 lockdown, while scores for the Dog-Owner Interaction subscale were significantly lower during the COVID-19 lockdown. Our study confirmed that service dogs, like other pets, were a source of emotional support for their owners during the COVID-19 lockdown. However, people with disabilities found their relationship with their service dog costlier (e.g., my dog makes too much mess). Our study highlights that, in extreme situations, characteristics of a human-animal relationship can be exacerbated in both positive and negative ways.

Cognitive performance is linked to survival in free-living African striped mice.

Cognition is shaped by evolution and is predicted to increase fitness. However, the link between cognition and fitness in free-living animals is unresolved. We studied the correlates of cognition and survival in a free-living rodent inhabiting an arid environment. We tested 143 striped mice (Rhabdomys pumilio) using a battery of cognitive tests, including: (i) an attention task, (ii) two problem-solving tasks, (iii) a learning and reversal learning task, and (iv) an inhibitory control task. We related cognitive performance with days of survival. Better problem-solving and inhibitory control performance were significant correlates of survival. Surviving males showed greater reversal learning which may be related to sex-specific behavioural and life-history characteristics. Specific cognitive traits and not a composite measure of general intelligence underpins fitness in this free-living rodent population, enhancing our understanding of the evolution of cognition in non-human animals.

Horses' Tactile Reactivity Differs According to the Type of Work: The Example of Equine-Assisted Intervention.

Tactile perception in humans varies between individuals and could depend on extrinsic factors such as working activity. In animals, there is no study relating the influence of animals' work and their tactile reactivity per se. We investigated horses' tactile reactivity using von Frey filament in different body areas and compared horses working only in equine-assisted interventions (EAI), in riding school (RS) lessons, and in both activities (EAI-RS). We further compared tactile actions by people with or without mental and/or developmental disorders during brushing sessions. The results indicated that EAI horses showed higher tactile reactivity compared to EAI-RS and RS horses, both in terms of number of reactions overall, and especially when the test involved thin filaments. All horses showed high tactile reactivity when tested on the stifle, and this was particularly true for EAI horses. These differences could be related to humans' actions, as participants diagnosed with disorders brushed more the hindquarters and showed more fragmented actions. This study opens new lines of thought on the influence of EAI working activity on horses' tactile reactivity, and hence, on horses' sensory perception. Tactile reactivity outside work, may be directly (via tactile stimulations) or indirectly (via the welfare state), influenced by working conditions.

Horses' attentional characteristics differ according to the type of work.

Attention is a central process of cognition and influences the execution of daily tasks. In humans, different types of work require different attentional skills and sport performance is associated with the ability to attention shift. Attention towards humans varies in dogs used for different types of work. Whether this variation is due to the recruitment of individuals suitable for specific types of work, or to the characteristics of the work, remains unclear. In the present study, we hypothesized that domestic horses (Equus caballus) trained for different types of work would also demonstrate different attentional characteristics but we also explored other possible factors of influence such as age, sex and breed. We exposed more than sixty horses, working in 4 different disciplines, and living in two types of housing conditions, to a visual attention test (VAT) performed in the home environment. Individual attentional characteristics in the test were not significantly influenced by age, sex, breed or conditions of life but were strongly related to the type of work. Riding school horses showed longer sequences and less fragmented attention than all other horses, including sport horses living in the same conditions. Interestingly, sport performance was correlated with attention fragmentation during the test in eventing horses, which may need more attention shifting during the competitions. Working conditions may influence attention characteristics indirectly through welfare, or directly through selection and training. Our study opens new lines of thought on the determinants of animal cognition and its plasticity and constitutes a further step towards understanding the interrelationship between working conditions and cognition.

Characteristics influencing local enhancement in free-living striped mice.

Social learning is widespread across species; however, we still know little about the impact of individual differences in behaviour on social transmission. We aimed to investigate factors influencing social learning in free-living Rhabdomys pumilio, a group-living, arid-adapted mouse. We studied 52 mice in a lid opening task in a field laboratory. We created observer-demonstrator dyads with demonstrators either opening lids or not. We measured success of observers to open lids, their attention and latency to open and time spent interacting with the device. We also considered influences of observer age, sex, group size and personality traits. Demonstrator success did not influence observer success, although attention towards the demonstrator did impact the observers' time spent with the device. Males were more successful than females and more active/explorative observers interacted with the device faster and for a longer time compared to less active/explorative counterparts. We found no influence of age and group size on mouse success. Striped mice appeared to use cues from other individuals to learn how to solve the task and it was influenced by sex and personality. Striped mice in this studied population may use local enhancement to acquire information socially.

Seasonal changes in problem-solving in wild African striped mice.

Innovative problem-solving ability is a predictor of whether animals can successfully cope with environmental changes. These environmental changes can test the limits of animals, for example when energy availability decreases seasonally and, hence, problem-solving performance decreases because less energy is available for cognitive processes. Here, we investigated: (1) how problem-solving performance changed between seasons that differed significantly in food availability; (2) whether these changes were related to environmentally induced physiological changes in blood glucose and ketone levels, indicators of energy availability; and (3) whether individual variation in problem-solving was related to sex differences. We studied 99 free-ranging African striped mice, Rhabdomys pumilio, in the Succulent Karoo, South Africa, 55 during the hot dry summer with low food availability and 44 during the cold wet winter with higher food availability. We measured their problem-solving abilities using a food extraction task and found no seasonal differences in problem-solving success. However, mice solved the problem faster in summer versus winter. In summer, food availability was reduced and blood ketones increased but there was no seasonal difference in blood glucose levels. There were no correlation between problem-solving performance and blood glucose or ketone levels. Overall, more males solved the task than females. It appears that in striped mice cognitive functions can be maintained under harsh environmental conditions.

Seasonal variation in reversal learning reveals greater female cognitive flexibility in African striped mice.

Cognitive flexibility describes the ability of animals to alter cognitively mediated behaviour in response to changing situational demands, and can vary according to prevailing environemental conditions and individual caracteristics. In the present study, we investigated (1) how learning and reversal learning performance changes between seasons, and (2) how cognitive flexibility is related to sex in a free-living small mammal. We studied 107 African striped mice, Rhabdomys pumilio, in an arid semi-desert, 58 during the hot dry summer with low food availability, and 49 during the cold wet winter with higher food availability. We used an escape box task to test for learning and reversal learning performance. We found that learning and reversal learning efficiency varied seasonally by sex: females tested in summer were faster at solving both learning and reversal tasks than males tested in winter. Performance varied within sex: males tested in winter showed faster learning compared to males tested in summer. During reversal learning, females tested in summer were more efficient and solve the task faster compared to females tested in winter. We suggest that seasonal cognitive performance could be related to sex-specific behavioural characteristics of the species, resulting in adaptation for living in harsh environmental conditions.

Interest in Humans: Comparisons between Riding School Lesson Equids and Assisted-Intervention Equids.

Little is known about the impact of equine-assisted interventions (EAI) on equids' perception of humans. In this study 172 equids, living in 12 riding centres, were submitted to a standardised human-horse relationship test: the motionless person test. Age, sex, type (horse/pony), housing, and feeding conditions of subjects were recorded. Overall, 17 equids worked in EAI, 95 in riding school lessons (RS), and 60 in both (EAI-RS). There were high inter-individual variations in the number of interactive behaviours directed towards the experimenter: negative binomial general linear models showed that activity was the most important factor: RS equids performed more interactive behaviours than EAI (p = 0.039) and EAI-RS (p < 0.001) equids. Daily quantity of hay appeared as the second most important factor (equids with more than 3 kg interacted more than equids with less than 3 kg, p = 0.013). Individual characteristics were also important as horses interacted more than ponies (p = 0.009), geldings more than mares (p = 0.032), and 3-15-year-old equids more than equids over 15 years (p = 0.032). However, there was no interaction between factors. The lower number of interactive behaviours of EAI equids leads to different hypotheses-namely, selection on temperament, specific training, or compromised welfare (apathy). In any case, our results raised new lines of questions on EAI.

Do alternative reproductive tactics predict problem-solving performance in African striped mice?

In changing environments, animals face unexpected problems to solve. Not all individuals in a population are equally able to solve new problems. It still remains unclear what factors (e.g. age and body condition) influence the propensity of problem solving. We investigated variation in problem-solving performance among males following alternative reproductive tactics (ARTs). We studied a free-ranging population of the African striped mouse (Rhabdomys pumilio). Adult male striped mice can employ 3 ARTs: (1) dominant group-living breeders, (2) philopatric living in their natal group, and (3) solitary-living roamers. ARTs in male striped mice reflect differences in competitiveness, sociality and physiology which could influence their problem-solving performance. We tested a total of 48 males in 2 years with two tasks: a string-pulling task to reach food and a door-opening task to reach the nest. Since male striped mice differ in personality traits independent of ARTs, we also measured activity, boldness and exploration. In addition, we assessed the association of body condition and age with problem solving. Problem solving was related the interaction of age and ARTs. The younger philopatrics had better performance in a food-extraction task whereas the older breeders were faster at solving the door-opening task. Individual differences in traits related to personality were significant correlates of problem-solving performance: pro-active mice (i.e. more active and explorative and bolder) performed better in both tasks. Finally, problem-solving performance was not consistent between the two tasks. Our study provides evidence of correlates of ARTs, age and personality on problem-solving abilities.

An Ambulatory Electroencephalography System for Freely Moving Horses: An Innovating Approach.

Electroencephalography (EEG) that has been extensively studied in humans presents also a large interest for studies on animal brain processes. However, since the quality of the recordings is altered by muscular activity, most EEG recordings on animals are obtained using invasive methods with deeply implanted electrodes. This requires anesthesia and can thus only be used in laboratory or clinical settings. As EEG is a very useful tool both for detecting brain alterations due to diseases or accidents and to evaluate the arousal and attentional state of the animal, it seemed crucial to develop a tool that would make such recordings possible in the horse's home environment, with a freely moving horse. Such a tool should neither be invasive nor cause discomforts to the horse as the usual other practice which consists, after shaving the zone, in gluing the electrodes to the skin. To fulfill these requirements, we developed a novel EEG headset adapted to the horse's head that allows an easy and fast positioning of the electrodes and that can be used in the home environment on a freely moving horse. In this study, we show that this EEG headset allows to obtain reliable recordings, and we propose an original evaluation of an animal's "EEG profile" that allows comparisons between individuals and situations. This EEG headset opens new possibilities of investigation on horse cognition, and it can also become a useful tool for veterinarians to evaluate cerebral disorders or check the anesthesia level during a surgery.

Depressive-like behavioral profiles in captive-bred single- and socially-housed rhesus and cynomolgus macaques: a species comparison.

BACKGROUND: To unravel the causes of major depressive disorder (MDD), the third leading cause of disease burden around the world, ethological animal models have recently been proposed. Our previous studies highlighted a depressive-like profile among single- and socially-housed farm-bred cynomolgus macaques. Although phylogenetically close, cynomolgus and rhesus macaques, the two most commonly used macaque species in biomedical research, differ on several levels such as patterns of aggression, reconciliation, temperament, or dominance styles. The question of whether one captive macaque species was more vulnerable than another in the development of a pathological profile reminiscent of MDD symptoms was explored. METHODS: Behavioral data (including body postures, orientations, gaze directions, inter-individual distances, and locations in the cage) were collected in farming conditions. Using an unbiased validated ethological scan-sampling method, followed by multiple correspondence and hierarchical clustering analyses, 40 single- and 35 socially-housed rhesus macaques were assessed. Independently, for each housing condition, inter-species comparisons were made with previously acquired data on farm-bred cynomolgus monkeys. RESULTS: Consistent with our previous studies, we found depressive-like characteristics (e.g., inactivity, low level of investigation and maintenance, long time spent inactive while facing the wall) among single- and socially-housed rhesus macaques. Species-specificities were reported in non-depressive time budgets and in the prevalence of the pathological profiles. CONCLUSIONS: Our results suggest that rhesus may be more vulnerable to developing a despair-like state than cynomolgus macaques, both in single- and in social-housing conditions. Therefore, rhesus macaques are more suitable for use as a "spontaneous" model of depressive disorders.

Birth origin differentially affects depressive-like behaviours: are captive-born cynomolgus monkeys more vulnerable to depression than their wild-born counterparts?

BACKGROUND: Adverse early-life experience might lead to the expression of abnormal behaviours in animals and the predisposition to psychiatric disorder (e.g. major depressive disorder) in Humans. Common breeding processes employ weaning and housing conditions different from what happens in the wild. METHODS: The present study, therefore, investigated whether birth origin impacts the possible existence of spontaneous atypical/abnormal behaviours displayed by 40 captive-born and 40 wild-born socially-housed cynomolgus macaques in farming conditions using an unbiased ethological scan-sampling analysis followed by multifactorial correspondence and hierarchical clustering analyses. RESULTS: We identified 10 distinct profiles (groups A to J) that significantly differed on several behaviours, body postures, body orientations, distances between individuals and locations in the cage. Data suggest that 4 captive-born and 1 wild-born animals (groups G and J) present depressive-like symptoms, unnatural early life events thereby increasing the risk of developing pathological symptoms. General differences were also highlighted between the captive- and wild-born populations, implying the expression of differential coping mechanisms in response to the same captive environment. CONCLUSIONS: Birth origin thus impacts the development of atypical ethologically-defined behavioural profiles, reminiscent of certain depressive-like symptoms. The use of unbiased behavioural observations might allow the identification of animal models of human mental/behavioural disorders and their most appropriate control groups.