Development of Optical Sensors Based on Quantum Dots Using Molecularly Imprinted Polymers for Determination of Prilocaine.

Optical sensors are analytical tools that able to provide analyte information. There are several ways to design optical sensors. This chapter presents an interesting optical sensor to detect prilocaine, a medicine, using quantum dots (QDs) combined with molecularly imprinted polymers (QDs@MIPs). This sensor simultaneously takes advantage of QDs and molecular imprinting technology, which enables the optical device to measure prilocaine with high selectivity and sensitivity. To prepare the optical sensor, CdTe QDs were used as fluorescent probes, and an imprinted silica polymer, as the recognition system, has been constructed on the QDs via sol-gel process to increase sensor selectivity.

Design a fluorometric aptasensor based on CoOOH nanosheets and carbon dots for simultaneous detection of lysozyme and adenosine triphosphate.

Simultaneous detection of biomarkers and biomolecules with great analytical performance still is challenging. A simple fluorometric dual-functional aptasensor was designed to detect Lysozyme (LYS) and adenosine triphosphate (ATP) as models of a protein and a small molecule simultaneously. The sensing principle of the aptasensor is based on the interactions between cobalt oxyhydroxide CoOOH nanosheets as fluorescence quencher and carbon dots (CDs) as fluorophores. The aptamer labeled with CDs was able to assemble on CoOOH nanosheets and consequently, the fluorescence signal was quenched. With addition target analytes to the system, the aptamers folded around of targets with a strong and specific affinity. Therefore, the labeled aptamer with CDs was detached from CoOOH nanosheets and the fluorescence signal was restored. The fluorescence spectral overlap of these two CDs is the main limitation for the simultaneous analysis. The least squared support vector machine (LS-SVM) was applied to resolve this problem. Under optimal conditions, when LS-SVR was used, detection limits were found 4.0 and 1.8 nmol L-1 for ATP and LYS. The parallel biosensor is capable of monitoring ATP and LYS levels in the biological samples with satisfactory results.

Fluorometric label-free aptasensor for detection of the pesticide acetamiprid by using cationic carbon dots prepared with cetrimonium bromide.

A fluorometric aptamer-based method is described for sensitive detection of the pesticide acetamiprid. Cationic carbon dots (cCDs) with blue fluorescence were synthesized from cetrimonium bromide (CTAB) by a hydrothermal method. In the presence of the acetamiprid aptamers with a negative charge, the aptamers bind to the surface of the cCDs due to electrostatic attraction. As a result, the fluorescence of the cCDs is quenched partially (the best measurement was done at excitation/emission wavelengths of 360/445 nm). If acetamiprid is added to the above system, the aptamer binds to acetamiprid as a target with strong and specific affinity. Therefore, fluorescence increases proportionally to the acetamiprid concentrations. The aptasensor has a detection limit of 0.3 nM with a dynamic range from 1.6 to 120 nM which reveals that the method is sensitive in comparison to the other techniques. The selectivity of the method towards various pesticides was also studied and found to be adequate. The sensor was applied for the determination of acetamiprid in (spiked) wastewater, tap water, and tomatoes to underpin its practicability. Graphical abstract Cationic CDs (cCDs) were synthesized from cetrimonium bromide by a hydrothermal method. The addition of the negatively charged acetamiprid aptamer to a solution containing cCDs, the cCDs will be coated by the aptamer. This causes the blue fluorescence of the cCDs partially is quenched. If acetamiprid (ACP) is then added, the aptamer will bind to acetamiprid with strong and specific affinity. Hence, fluorescence will be gradually restored.

DNA G-quadruplexes binding and antitumor activity of palladium aryl oxime ligand complexes encapsulated in either albumin or algal cellulose nanoparticles.

The interactions between two Pd complexes, designated as [Pd3(C,N-(C6H4C(Cl) = NO)-4)6] (complex 1) and [Pd3(C12H8C = NO)6] (complex 2), with the human telomeric G-quadruplex DNA, 5'-G3(T2AG3)3-3' (HTG21), were monitored using spectroscopic, biological, and molecular modeling studies. According to the UV-vis results, these complexes can strongly induce and stabilize G-quadruplex DNA structure with Kb1 = 4.5(±0.3) × 106 M-1 and Kb2 = 1.0(±0.2) × 107 M-1via groove mode in comparison with duplex DNA. The release mechanism of the Pd complexes from BSA nanoparticles followed a biphasic pattern unlike that of algal cellulose nanoparticles in vitro. In addition, the cytotoxicity of these complexes on MCF-7 cancer cells and PBMC normal cells was evaluated and compared with cisplatin under similar experimental conditions. Furthermore, to determine and verify the interaction mode of these compounds with G-quadruplex, the molecular docking technique was also performed. Our data clearly demonstrated that complex 2 had higher activity and cytotoxicity than that of complex 1 and could be further investigated in the future as a drug discovery platform.

A fluorometric aptasensor for methamphetamine based on fluorescence resonance energy transfer using cobalt oxyhydroxide nanosheets and carbon dots.

Cobalt oxyhydroxide (CoOOH) nanosheets are efficient fluorescence quenchers due to their specific optical properties and high surface area. The combination of CoOOH nanosheets and carbon dots (CDs) has not been used in any aptasensor based on fluorescence quenching so far. An aptamer based fluorometric assay is introduced that is making use of fluorescent CDs conjugated to the aptamer against methamphetamine (MTA), and of CoOOH nanosheets which reduce the fluorescence of the CDs as a quencher. The results revealed that the conjugated CDs with aptamers were able to enclose the CoOOH nanosheets. Consequently, fluorescence is quenched. If the aptamer on the CD binds MTA, the CDs are detached from CoOOH nanosheets. As a result, fluorescence is restored proportionally to zhe MTA concentration. The fluorometric limit of detection is 1 nM with a dynamic range from 5 to 156 nM. The method was validated by comparing the results obtained by the new method to those obtained by ion mobility spectroscopy. Theoretical studies showed that the distance between CoOOH nanosheet and C-Ds is approximately 7.6 Å which can illustrate the possibility of FRET phenomenon. The interactions of MTA and the aptamer were investigated using molecular dynamic simulation (MDS). Graphical abstract Carbon dots (C-Ds) were prepared from grape leaves, conjugated to aptamer, and adsorbed on CoOOH nanosheets. So, the fluorescence of C-Ds is quenched. On addition of MTA, fluorescence is restored.

A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer-magnetic nanoparticles and its single-stranded complementary DNA labeled carbon dots.

A new fluorimetric aptasensor was designed for the determination of adenosine triphosphate (ATP) based on magnetic nanoparticles (MNPs) and carbon dots (CDs). In this analytical strategy, an ATP aptamer was conjugated on MNPs and a complementary strand of the aptamer (CS) was labeled with CDs. The aptamer and its CS were hybridized to form a double helical structure. The hybridized aptamers could be used for the specific recognition of ATP in a biological complex matrix using a strong magnetic field to remove the interfering effect. In the absence of ATP, no CDs-CS could be released into the solution and this resulted in a weak fluorescence signal. In the presence of ATP, the target binds to its aptamer and causes the dissociation of the double helical structure and liberation of the CS, such that a strong fluorescence signal was generated. The increased fluorescence signal was proportional to ATP concentration. The limit of detection was estimated to be 1.0 pmol L-1 with a dynamic range of 3.0 pmol L-1 to 5.0 nmol L-1 . The specific aptasensor was applied to detect ATP in human serum samples with satisfactory results. Moreover, molecular dynamic simulation (MDS) studies were used to analyze interactions of the ATP molecule with the aptamer.