A Microwave Voyage into Swelling Phenomenon: Investigation of Polydimethylsiloxane and VOCs Interaction.

When exposed to specific gases, polymers undergo swelling, leading to physiochemical changes that can significantly affect their performance. Monitoring this swelling phenomenon requires innovative approaches. This study focuses on investigating the real-time resonant microwave behavior of two polydimethylsiloxane (PDMS) structures (solid and porous) in interaction with tetrahydrofuran (THF) and acetone, which are primary swelling agents. A microwave measurement method is proposed using an 8.63 GHz planar split ring resonator (SRR). The device's resonant frequency downshifts to 7.75 and 8.42 GHz when solid and porous PDMS blocks are placed on the split ring gap. Interaction of the solid PDMS and porous PDMS with target gases caused a change in PDMS structure resulting in alterations in the dielectric properties of the PDMS/gas system, as evidenced by the resonator's transmission amplitude and resonant frequency shifts. The magnitude of these shifts depends on the type and concentration of the solvent gas. The PDMS-integrated SRR exhibits a sensitivity of 25.3 MHz/1 ppt THF and 7 MHz/1 ppt acetone. Additionally, the solid block demonstrates response times of 6800 and 4200 s for swelling and deswelling, respectively, when in exposure to 25 ppt concentrations of THF and acetone. Overall, this study underscores the substantial potential of microwave resonators as versatile tools for investigating the physical changes in polymers during their interaction with gases, contributing to the understanding of polymer-gas interactions and opening avenues for further research and diverse applications.

A comprehensive study on Industry 4.0 in the pharmaceutical industry for sustainable development.

The four evolutionary stages have brought us to Industry 4.0. Industry 4.0 is nothing but the 4th Industrial Revolution which will change the production processes. The implementation of Industry 4.0 in the pharmaceutical sector will make the manufacturing of complex drugs easier. The arrival of Industry 4.0 and its advanced technologies such as artificial intelligence (AI), robotics, and the Internet of Things (IoT) makes the processes flexible. Industry 4.0 was introduced to reduce the human workforce and make the complicated processes unchallenging. It is used in all aspects of pharmaceutical sector like analysis, diagnosis, manufacturing, and packaging. The main aim of this paper is to comprehensively elucidate how Industry 4.0 has played a significant role in sustainable development (SD). Industry 4.0 in sustainability decreases the research efforts and examines the research sector's opportunities. This paper also discusses the impact of Industry 4.0 on sustainable development. Industry 4.0 constructs a bridge between industry and sustainability leading to sustainable development. Sustainability can be achieved by adopting innovative techniques of Industry 4.0 in manufacturing. Moreover, Industry 4.0 provides potential benefits for enhancing pharmaceutical production concerning flexibility, expenses, standards, and safety. It is noticed that Industry 4.0 has a beneficial impact on sustainable development by implementing advanced technologies leading to flexible manufacturing processes.

PANI-wrapped BaFe12O19 and SrFe12O19 with rGO composite materials for electromagnetic interference shielding applications.

We report electromagnetic interference (EMI) shielding efficiency in the PANI-wrapped BaFe12O19 and SrFe12O19 with rGO composites. Barium and strontium hexaferrites were synthesized using the nitrate citrate gel combustion method. These hexaferrites were polymerized in situ with aniline. The PANI-coated ferrite-based composite materials were developed along with reduced graphene oxide (rGO) in acrylonitrile butadiene styrene (ABS) polymer, and their shielding effectiveness was assessed in X-band frequency range (8.2-12.4 GHz). The reflection (SER) and absorption (SEA) mechanism of shielding effectiveness was discussed with the different rGO concentrations. The results reveal that 5 wt% of rGO with PANI-coated barium and strontium hexaferrite polymer composites exhibit shielding efficiency of 21.5 dB and 19.5 dB, respectively, for 1 mm thickness composite. These hexaferrite polymer-based composite materials can be used as an attractive candidate for EM shielding materials in various technological applications.

Single Breath Counting for Diagnosis and Prediction of Severity of Acute Asthma Exacerbation in Children.

OBJECTIVES: To find correlation between single breath counting (SBC) and spirometry in acute exacerbation of asthma in children. METHODS: A cross-sectional observational study including all children aged 6-12 y age, who presented in outpatient department or emergency room with acute exacerbation of asthma, not fitting in exclusion criteria were enrolled. SBC and spirometry parameters were obtained before treatment and 1 h after initiating treatment (GINA 2017 Guidelines). RESULTS: Significant correlation was observed in SBC and FVC (r = 0.349), FEV1 (r = 0.439), FEF25-75 (r = 0.424), and PEF (r = 0.593). Cutoff value of SBC of 23 was found to be suggestive of severe/life-threatening asthma with sensitivity of 83.3% and specificity of 84.4%. No significant association was found between percent change in SBC and spirometry parameters in response to treatment. CONCLUSIONS: SBC can be used to predict PEF, FEV1, FVC, and FEF25-75 of a patient aged between 6 and 12 y during acute asthma exacerbation. The observed cutoff value of 23 of SBC score has a good sensitivity and specificity to identify life-threatening/severe exacerbation. SBC score significantly increases in response to therapy.

Pattern of pediatric emergency visits during lockdown -Impact of COVID-19 pandemic in Jodhpur City, India.

Objectives: To study the impact of lockdown during novel coronavirus disease-19 (COVID-19) pandemic on Pediatric Emergency visits. Methods: In this retrospective study, we compared the data regarding demographic details, triage category, underlying primary organ system involvement and outcomes among pediatric emergency visits of April-May 2019 and 2020. Results are expressed in means and proportions. Chi-square test was applied to compare the proportion in two groups. Results: We observed decrease in number of patients during lockdown period as compared to previous year (792 vs 506, decrease by 36%). There was significant increase in respiratory (P = 0.001, CI 2.66-11.6), and hematological emergencies (P < 0.001, CI 2.96-9.23) Road traffic accident (RTA) were decreased significantly (P = 0.005, CI 0.6-3.06). Unintentional injuries were comparable to previous year (P = 0.14, CI -0.86-6.19), though children being at home. Conclusion: During lockdown, number of pediatric emergency visits was reduced and also lead to delayed presentation. Parents should be informed about utilizing telemedicine services available during this period and avoid delaying in going to hospitals in emergency conditions.

Spatial Transcriptomics of Nematodes Identifies Sperm Cells as a Source of Genomic Novelty and Rapid Evolution.

Divergence of gene function and expression during development can give rise to phenotypic differences at the level of cells, tissues, organs, and ultimately whole organisms. To gain insights into the evolution of gene expression and novel genes at spatial resolution, we compared the spatially resolved transcriptomes of two distantly related nematodes, Caenorhabditis elegans and Pristionchus pacificus, that diverged 60-90 Ma. The spatial transcriptomes of adult worms show little evidence for strong conservation at the level of single genes. Instead, regional expression is largely driven by recent duplication and emergence of novel genes. Estimation of gene ages across anatomical structures revealed an enrichment of novel genes in sperm-related regions. This provides first evidence in nematodes for the "out of testis" hypothesis that has been previously postulated based on studies in Drosophila and mammals. "Out of testis" genes represent a mix of products of pervasive transcription as well as fast evolving members of ancient gene families. Strikingly, numerous novel genes have known functions during meiosis in Caenorhabditis elegans indicating that even universal processes such as meiosis may be targets of rapid evolution. Our study highlights the importance of novel genes in generating phenotypic diversity and explicitly characterizes gene origination in sperm-related regions. Furthermore, it proposes new functions for previously uncharacterized genes and establishes the spatial transcriptome of Pristionchus pacificus as a catalog for future studies on the evolution of gene expression and function.

Graphene templated growth of copper sulphide 'flowers' can suppress electromagnetic interference.

With increasing usage of electronic gadgets in various fields, the problem of electromagnetic interference (EMI) has become eminent. To suppress this interference, lightweight materials that are non-corrosive in nature and easy to fabricate, design, integrate and process are in great demand. In the present study, we have grown copper sulphide 'flowers' on graphene oxide by a facile one pot hydrothermal technique. The growth time of the "flower-like" structure was optimised based on structural (XRD) and morphological analysis (SEM). Then, the as-prepared structures were dispersed in a PVDF matrix using melt blending. The bulk AC electrical conductivity and EMI shielding ability of the prepared composite were assessed, and it was observed that the nanocomposites exhibited an EMI shielding effectiveness up to -25 dB manifesting in 86% absorption of the incoming EM waves at a thickness of only 1 mm. Moreover, it was also observed that addition of hybrid nanoparticles has a better effect on the electromagnetic (EM) shielding performance compared to when the nanoparticles are added separately in terms of both total shielding effectiveness as well as absorption performance. A minimum skin depth of 0.38 mm was observed in the case of the hybrid nanostructure.

Crowdsourcing and the feasibility of manual gene annotation: A pilot study in the nematode Pristionchus pacificus.

Nematodes such as Caenorhabditis elegans are powerful systems to study basically all aspects of biology. Their species richness together with tremendous genetic knowledge from C. elegans facilitate the evolutionary study of biological functions using reverse genetics. However, the ability to identify orthologs of candidate genes in other species can be hampered by erroneous gene annotations. To improve gene annotation in the nematode model organism Pristionchus pacificus, we performed a genome-wide screen for C. elegans genes with potentially incorrectly annotated P. pacificus orthologs. We initiated a community-based project to manually inspect more than two thousand candidate loci and to propose new gene models based on recently generated Iso-seq and RNA-seq data. In most cases, misannotation of C. elegans orthologs was due to artificially fused gene predictions and completely missing gene models. The community-based curation raised the gene count from 25,517 to 28,036 and increased the single copy ortholog completeness level from 86% to 97%. This pilot study demonstrates how even small-scale crowdsourcing can drastically improve gene annotations. In future, similar approaches can be used for other species, gene sets, and even larger communities thus making manual annotation of large parts of the genome feasible.

Environmental influence on Pristionchus pacificus mouth form through different culture methods.

Environmental cues can impact development to elicit distinct phenotypes in the adult. The consequences of phenotypic plasticity can have profound effects on morphology, life cycle, and behavior to increase the fitness of the organism. The molecular mechanisms governing these interactions are beginning to be elucidated in a few cases, such as social insects. Nevertheless, there is a paucity of systems that are amenable to rigorous experimentation, preventing both detailed mechanistic insight and the establishment of a generalizable conceptual framework. The mouth dimorphism of the model nematode Pristionchus pacificus offers the rare opportunity to examine the genetics, genomics, and epigenetics of environmental influence on developmental plasticity. Yet there are currently no easily tunable environmental factors that affect mouth-form ratios and are scalable to large cultures required for molecular biology. Here we present a suite of culture conditions to toggle the mouth-form phenotype of P. pacificus. The effects are reversible, do not require the costly or labor-intensive synthesis of chemicals, and proceed through the same pathways previously examined from forward genetic screens. Different species of Pristionchus exhibit different responses to culture conditions, demonstrating unique gene-environment interactions, and providing an opportunity to study environmental influence on a macroevolutionary scale.