Probiotics enhance alveolar bone microarchitecture, intestinal morphology and estradiol levels in osteoporotic animals.

BACKGROUND AND OBJECTIVE: Osteoporosis is associated with bone microarchitecture alterations, and the depletion of estrogen during menopause is a major contributing factor to its development. The literature highlights the noteworthy role of gut microbiota in bone metabolism, particularly in the progression of osteoporosis. Periodontal disease leads to alveolar bone loss, which may be influenced by estrogen deficiency, and this mechanism is intricately associated with an imbalance in systemic microbiota. The aim of this study was to evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (B. lactis HN019) and Lacticaseibacillus casei 01 (L. casei 01) administrations on an osteoporosis animal model. MATERIALS AND METHODS: Thirty-three female rats were randomly divided into three groups: control (C-OVX), C-OVX-HN019 and C-OVX-LC01. All animals were ovariectomized. In groups C-OVX-HN019 and C-OVX-LC01, the probiotics were administered for 4 months. All animals were euthanized after 16 weeks from ovariectomy. Microtomographic, histopathological and immunohistochemical examinations were conducted on periodontal tissues, whereas histomorphometry, histopathological and immunohistochemical analyses were carried out on the intestine. The levels of estradiol were assessed in blood using an immunoenzymatic assay. The data were subjected to statistical analyses (p < .05). RESULTS: The C-OVX-LC01 group exhibited a significant reduction in alveolar bone porosity and an increase in connective tissue density compared to C-OVX (p < .05). The C-OVX-HN019 and C-OVX-LC01 groups presented reduced expression of TRAP and RANKL compared to the C-OVX (p < .05). The C-OVX group presented villi defects, mild neutrophil infiltration, decrease in both villous height and intestinal crypts and reduced expression of intestinal junctional epithelium markers e-cadherin and claudin 01 compared to C-OVX-HN019 and C-OVX-LC01 (p < .05). The C-OVX group had lower estradiol levels than C-OVX-HN019 and C-OVX-LC01 (p < .05). CONCLUSION: The probiotic therapy promoted a reduction in alveolar bone destruction and intestinal permeability as well as an increase in estradiol levels in ovariectomized rats. Specifically, the probiotic strain Lacticaseibacillus casei 01 exhibited greater effectiveness compared to Bifidobacterium animalis subsp. lactis HN019, indicating strain-dependent outcomes.

Effects of Bifidobacterium animalis subsp. lactis HN019 on ligature-induced periodontitis in rats with experimental rheumatoid arthritis.

The purpose of this study was to evaluate the effects of systemic administration of the probiotic Bifidobacterium animalis subsp. lactis HN019 (HN019) on ligature-induced periodontitis in rats with experimental rheumatoid arthritis (RA). 28 rats were divided into four groups (n=7): RA (rheumatoid arthritis), RA/PROB (probiotic), RA/EP (experimental periodontitis) and RA/EP/PROB. From day zero, HN019 was added daily to the water of the PROB groups animals until the end of the experiment. From day seven, RA was induced. On day 28, in EP groups, ligatures were positioned around mandibular first molars and remained in position for 11 days, in order to induce periodontitis. The animals were euthanised on day 39. Microtomographic, histomorphometric, immunoenzymatic and microbiological analyses were performed. Data were statistically analysed (P<0.05). Group RA/EP/PROB presented reduced alveolar bone loss, tumour necrosis factor-α and interleukin (IL)-6 levels and increased IL-17 levels when compared with group RA/EP. There were no significant differences regarding connective tissue attachment level and IL-10 levels between groups RA/EP and RA/EP/PROB. Group RA/PROB showed decreased anti-citrullinated protein antibodies levels when compared with groups RA and RA/EP. Group RA/EP/PROB presented a higher rate of aerobic/anaerobic bacteria than group RA/EP. Systemic administration of HN019 promoted a protective effect against periodontal tissue destruction, decreasing both bone loss and inflammatory mediators and increasing the proportion of bacteria compatible with periodontal health, in rats with experimental RA and EP.

Postprandial glycemia in healthy subjects: Which probiotic dairy food is more adequate?

The consumption of probiotic-enriched dairy products has been associated with many health benefits, including anti-hyperglycemic activity. The effect on health is dependent on the type of probiotic culture used and the dairy product consumed. This study evaluated the effect of different probiotic-enriched dairy matrices (Minas Frescal cheese, Prato cheese, and whey dairy beverage) containing Lactobacillus casei on in vitro and in vivo anti-hyperglycemic activity. For this purpose, in vitro anti-hyperglycemic activity was determined by the inhibition of α-glucosidase and α-amylase activities, and a human study was performed with healthy individuals (n = 15, consumption of bread as a control; bread + Minas Frescal cheese; bread + Prato cheese; bread + dairy beverage) to assess the effects of different probiotic foods on postprandial glycemia. In vitro data showed that Prato cheese presented the highest lipid (36.9 g/100 g) and protein (26.5 g/100 g) contents as well as the highest α-amylase (60.7%) and α-glucosidase (52.6%) inhibition. The consumption of Prato cheese resulted in a lesser increase in blood glucose level (13 mg/dL) compared with the consumption of bread alone (19 mg/dL), Minas Frescal cheese (20 mg/dL), and whey dairy beverage (30 mg/dL), with glycemic indices similar to that observed for the control. The present results demonstrated a good correlation between in vitro and in vivo data, in which the type of dairy matrix affects the anti-hyperglycemic activity. It is concluded that the consumption of probiotic Prato cheese can contribute to the reduction of postprandial glycemia in healthy individuals.

Análise bioeconômica de períodos de suplementação proteico-energética na estação seca para novilhas Nelore em pastagem diferida de Urochloa decumbens / Bioeconomic analysis of protein-energy supplementation periods in dry season for Nellore heifers in stockpiled pastures of Urochloa decumbens

A suplementação proteico-energética (SPE) é uma estratégia adequada à bovinocultura de corte no período seco do ano, embora sua adoção equivocada possa comprometer seu benefício/custo. O objetivo deste estudo foi avaliar o desempenho de novilhas Nelore em pastagem diferida de Urochloa decumbens sob dois períodos de SPE, e realizar análise econômica desses sistemas de produção. O experimento foi conduzido sob delineamento inteiramente ao acaso, com 147 e 55 dias de SPE (sistemas 1 e 2, respectivamente), de 21/06 a 15/11/2016, com 16 e 20 novilhas nos sistemas 1 e 2, respectivamente. Os dados de desempenho foram analisados utilizando-se o PROC MIXED do SAS®, e as médias comparadas por PDIFF (P<0,05). Os pesos médios foram semelhantes (P>0,05) entre os sistemas de produção nas pesagens de 21/06, 12/07, 02/08, 23/08 e 15/11/2016. Os valores presentes líquidos foram positivos sob a taxa anual de desconto de 4%, sendo viáveis financeiramente. Por apresentarem taxa interna de retorno superior à taxa mínima de atratividade, os sistemas foram atrativos para investimento. Foi obtido balanço econômico positivo de R$ 25,26/animal para substituição do sistema 1 pelo sistema 2. A redução de 92 dias na SPE proporciona desempenho semelhante entre novilhas e maior eficiência econômica do sistema de produção.(AU)

Protein-energy supplementation (SPE) is an adequate strategy for beef cattle during drought season of the year, although the wrong adoption might compromise its cost/benefit ratio. The aim of this study was to evaluate Nellore heifers' performance under SPE periods and to draw economic analysis of production systems. The experiment was conducted under a completely randomized design, with two systems: 147 and 55 days of SPE (Systems 1 and 2), from 06/21/2016 to 11/15/2016, with 16 and 20 heifers to Systems 1 and 2, respectively. Performance data were analyzed by SAS® PROC MIXED and means compared by PDIFF (P< 0.05). The weight means were similar between the production systems (P> 0.05) at weighing of 06/21, 07/12, 08/02, 08/23 and 11/15/2016. The net present values were positive under annual discount rate of 4%, being financially viable. By presenting internal return rate higher than the minimum attractiveness rate, the systems were attractive for investment. Positive economic balance was obtained of R$ 25.26/animal to replace System 1 by 2. The reduction of 92 days in SPE provides similar performance between Nellore heifers and greater economic efficiency of the production system.(AU)

Análise bioeconômica de períodos de suplementação proteico-energética na estação seca para novilhas Nelore em pastagem diferida de Urochloa decumbens / Bioeconomic analysis of protein-energy supplementation periods in dry season for Nellore heifers in stockpiled pastures of Urochloa decumbens

A suplementação proteico-energética (SPE) é uma estratégia adequada à bovinocultura de corte no período seco do ano, embora sua adoção equivocada possa comprometer seu benefício/custo. O objetivo deste estudo foi avaliar o desempenho de novilhas Nelore em pastagem diferida de Urochloa decumbens sob dois períodos de SPE, e realizar análise econômica desses sistemas de produção. O experimento foi conduzido sob delineamento inteiramente ao acaso, com 147 e 55 dias de SPE (sistemas 1 e 2, respectivamente), de 21/06 a 15/11/2016, com 16 e 20 novilhas nos sistemas 1 e 2, respectivamente. Os dados de desempenho foram analisados utilizando-se o PROC MIXED do SAS®, e as médias comparadas por PDIFF (P<0,05). Os pesos médios foram semelhantes (P>0,05) entre os sistemas de produção nas pesagens de 21/06, 12/07, 02/08, 23/08 e 15/11/2016. Os valores presentes líquidos foram positivos sob a taxa anual de desconto de 4%, sendo viáveis financeiramente. Por apresentarem taxa interna de retorno superior à taxa mínima de atratividade, os sistemas foram atrativos para investimento. Foi obtido balanço econômico positivo de R$ 25,26/animal para substituição do sistema 1 pelo sistema 2. A redução de 92 dias na SPE proporciona desempenho semelhante entre novilhas e maior eficiência econômica do sistema de produção.(AU)

Protein-energy supplementation (SPE) is an adequate strategy for beef cattle during drought season of the year, although the wrong adoption might compromise its cost/benefit ratio. The aim of this study was to evaluate Nellore heifers' performance under SPE periods and to draw economic analysis of production systems. The experiment was conducted under a completely randomized design, with two systems: 147 and 55 days of SPE (Systems 1 and 2), from 06/21/2016 to 11/15/2016, with 16 and 20 heifers to Systems 1 and 2, respectively. Performance data were analyzed by SAS® PROC MIXED and means compared by PDIFF (P< 0.05). The weight means were similar between the production systems (P> 0.05) at weighing of 06/21, 07/12, 08/02, 08/23 and 11/15/2016. The net present values were positive under annual discount rate of 4%, being financially viable. By presenting internal return rate higher than the minimum attractiveness rate, the systems were attractive for investment. Positive economic balance was obtained of R$ 25.26/animal to replace System 1 by 2. The reduction of 92 days in SPE provides similar performance between Nellore heifers and greater economic efficiency of the production system.(AU)

Effect of curcumin on bone tissue in the diabetic rat: repair of peri-implant and critical-sized defects.

This study determined the effect of curcumin on bone healing in animals with diabetes mellitus (DM). One hundred rats were divided into five groups: DM+PLAC, DM+CURC, DM+INS, DM+CURC+INS, and non-DM (CURC, curcumin; PLAC, placebo; INS, insulin). Critical calvarial defects were created and titanium implants were inserted into the tibiae. Calvarial defects were analyzed histometrically, and BMP-2, OPN, OPG, RANKL, Runx2, Osx, ß-catenin, Lrp-5, and Dkk1 mRNA levels were quantified by PCR. The implants were removed for a torque evaluation, the peri-implant tissue was collected for mRNA quantification of the same bone-related markers, and the tibiae were submitted to micro-computed tomography. The DM+CURC+INS and non-DM groups exhibited greater closure of the calvaria when compared to the DM+PLAC group (P<0.05). Increased retention of implants was observed in the DM+CURC, DM+CURC+INS, and non-DM groups when compared to the DM+PLAC group (P<0.05). CURC improved bone volume and increased bone-implant contact when compared to DM+PLAC (P<0.05). In calvarial samples, CURC favourably modulated RANKL/OPG and Dkk1 and improved ß-catenin levels when compared to DM+PLAC (P<0.05). In peri-implant samples, Dkk1 and RANKL/OPG were down-regulated and BMP-2 up-regulated by CURC when compared to DM+PLAC (P<0.05). CURC reverses the harmful effects of DM in bone healing, contributing to the modulation of bone-related markers.

Perfil de ácidos graxos no leite de vacas holandês em pastagens tropicais suplementadas com dois níveis de concentrado / Milk fatty acids profile of Holstein cows grazing tropical forages supplemented with two concentrate levels

Milk fatty acids profile was evaluated using 12 Holstein cows producing 15 kg/day of milk with 4.0% of fat. The animals were assigned to four treatments in a 2 x 2 factorial arrangement (two tropical grasses - Brachiaria brizantha cv. Xaraés and Panicum maximum cv. Tanzania, supplemented with two concentrate levels - 3 and 6 kg/cow/day) in a completely randomized block design. There was no effect of forage and concentrate level (P>0.05) on concentrations of rumenic, vaccenic, (C18: 1 trans-11) and oleic acids in milk fat (mean values of 1.01, 1.79, and 22.55 g/100 g of total fatty acids, respectively). The concentrations of lauric (C12:0), myristic (C14:0), and palmitic (C16:0) acids were also unaffected by treatments (mean values of 2.63, 9.77, and 27.44g/100g of total fatty acids, respectively). Overall, the results illustrate the potential of tropical grasses in producing milk containing a more desirable fatty acids profile in terms of human health.

Perfil de ácidos graxos no leite de vacas Holandês em pastagens tropicais suplementadas com dois níveis de concentrado / Milk fatty acids profile of Holstein cows grazing tropical forages supplemented with two concentrate levels

Milk fatty acids profile was evaluated using 12 Holstein cows producing 15 kg/day of milk with 4.0% of fat. The animals were assigned to four treatments in a 2 x 2 factorial arrangement (two tropical grasses - Brachiaria brizantha cv. Xaraés and Panicum maximum cv. Tanzania, supplemented with two concentrate levels - 3 and 6 kg/cow/day) in a completely randomized block design. There was no effect of forage and concentrate level (P>0.05) on concentrations of rumenic, vaccenic, (C18: 1 trans-11) and oleic acids in milk fat (mean values of 1.01, 1.79, and 22.55 g/100 g of total fatty acids, respectively). The concentrations of lauric (C12:0), myristic (C14:0), and palmitic (C16:0) acids were also unaffected by treatments (mean values of 2.63, 9.77, and 27.44g/100g of total fatty acids, respectively). Overall, the results illustrate the potential of tropical grasses in producing milk containing a more desirable fatty acids profile in terms of human health.(AU)