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1.
Vector Borne Zoonotic Dis ; 23(11): 576-582, 2023 11.
Article in English | MEDLINE | ID: mdl-37695815

ABSTRACT

Background: The objective of this study was to isolate Staphylococcus spp. and to characterize the resistance profile in nasal samples from pigs slaughtered for consumption. Material and Methods: Intranasal swabs were collected from 100 pigs immediately after bleeding in a slaughterhouse located in the largest pork production region in Brazil, these samples were cultured and isolated to identify Staphylococcus spp. in coagulase positive (CoPS) and coagulase negative (CoNS) and molecular identification of Staphylococcus aureus and then subjected to the disk-diffusion test to identify the bacterial resistance profile and search for the mecA gene. Results: Of the 100 samples collected, it was possible to isolate 79 Staphylococcus spp., of these, 72.15% were classified as CoNS and 27.85% of the isolates classified as CoPS. Among the CoPS isolates, 77.27% were identified as S. aureus. Through the disk-diffusion test, it was possible to verify isolates resistant to clindamycin and erythromycin (98.73%), chloramphenicol (93.67%), and doxycycline (89.87%). There was amplification of the mecA gene in 30.38% of Staphylococcus spp. Conclusion: The results of this study highlight the need for the careful use of antibiotics in swine production, in addition to aiming at continuous surveillance in relation to the rate of multiresistant microorganisms within these environments, focused on large industrial centers; such results also indicate the importance of understanding, through future studies, possible pathways to transmission of these microorganisms directly, or indirectly, through meat products derived from these pigs, which can be considered neglected diffusers of variants of Staphylococcus spp. resistant to antibiotics or carriers of important resistance genes related to One Health.


Subject(s)
Staphylococcal Infections , Swine Diseases , Animals , Swine , Staphylococcus/genetics , Staphylococcus aureus/genetics , Coagulase/metabolism , Bacterial Proteins/genetics , Microbial Sensitivity Tests/veterinary , Anti-Bacterial Agents/pharmacology , Staphylococcal Infections/epidemiology , Staphylococcal Infections/veterinary , Staphylococcal Infections/microbiology , Swine Diseases/epidemiology
2.
Semina Ci. agr. ; 39(1): 167-176, jan.-fev. 2018. tab, mapas
Article in English | VETINDEX | ID: vti-728475

ABSTRACT

Dogs can act as intermediary hosts, reservoirs, and sentinel animals for zoonotic diseases such as brucellosis, toxoplasmosis and leptospirosis, and human contact with domestic animals can spread these infections. These diseases are globally distributed, and are a uniquely severe health issue, since they can infect a great range of animals, including humans. The purpose of this work was to determine the prevalence of anti-Leptospira spp., anti-Brucella spp., and anti-Toxoplasma gondii antibodies in stray dogs. Blood samples were collected from 181 stray dogs and used for serological diagnosis. Of the analyzed samples, 36.46%, 16.57%, and 9.39% were positive for T. gondii, Leptospira spp., and B. canis. The results indicate that these zoonotic diseases are highly prevalent in stray dogs in the northwestern region of the state of Paraná. The high infection rates for these zoonotics in the canine population is an indication that the environment is contaminated with a variety of different microorganisms, exposing both humans and dogs to different sources of infection.(AU)


O contato do homem com alguns animais domésticos faz com que algumas zoonoses como brucelose, toxoplasmose e leptospirose, tornem-se infecções importantes e, com isso os cães atuam como hospedeiros intermediários, reservatórios e animais sentinelas para estas infecções. Essas enfermidades tem distribuição mundial, e é um grave problema de saúde pública, podendo infectar uma grande variedade de animais, entres os quais, se destacam o cão e o homem. O objetivo deste trabalho foi detectar a ocorrência de anticorpos contra Leptospira spp., Brucella spp. eToxoplasma gondii em cães errantes Amostras de sangue colheram amostras de sangue de 181 cães errantes para a realização dos diagnósticos sorológicos. Das amostras analisadas, 36,46% foram reagentes para Tgondii, 16,57% para Leptospira spp. e9,39% para Brucella canis.A partir dos resultados obtidos no presente trabalho, verificou-se que essas zoonoses apresentam elevadaprevalência nos cães errantes do estudo. O elevado índice de infecção da população canina para estas zoonoses, torna-seum indicativo de que o ambiente está contaminado com diferentes micro-organismos representando um problema para a saúde única, uma vez que tanto o homem como o cão estão expostos a diferentes fontes de infecção.(AU)


Subject(s)
Animals , Dogs , Antibodies , Leptospira interrogans serovar canicola/immunology , Brucella canis/immunology , Toxoplasma/immunology , Leptospirosis , Toxoplasmosis, Animal , Brucellosis/veterinary , Zoonoses
3.
Semina ciênc. agrar ; 39(1): 167-176, jan.-fev. 2018. tab, map
Article in English | VETINDEX | ID: biblio-1501066

ABSTRACT

Dogs can act as intermediary hosts, reservoirs, and sentinel animals for zoonotic diseases such as brucellosis, toxoplasmosis and leptospirosis, and human contact with domestic animals can spread these infections. These diseases are globally distributed, and are a uniquely severe health issue, since they can infect a great range of animals, including humans. The purpose of this work was to determine the prevalence of anti-Leptospira spp., anti-Brucella spp., and anti-Toxoplasma gondii antibodies in stray dogs. Blood samples were collected from 181 stray dogs and used for serological diagnosis. Of the analyzed samples, 36.46%, 16.57%, and 9.39% were positive for T. gondii, Leptospira spp., and B. canis. The results indicate that these zoonotic diseases are highly prevalent in stray dogs in the northwestern region of the state of Paraná. The high infection rates for these zoonotics in the canine population is an indication that the environment is contaminated with a variety of different microorganisms, exposing both humans and dogs to different sources of infection.


O contato do homem com alguns animais domésticos faz com que algumas zoonoses como brucelose, toxoplasmose e leptospirose, tornem-se infecções importantes e, com isso os cães atuam como hospedeiros intermediários, reservatórios e animais sentinelas para estas infecções. Essas enfermidades tem distribuição mundial, e é um grave problema de saúde pública, podendo infectar uma grande variedade de animais, entres os quais, se destacam o cão e o homem. O objetivo deste trabalho foi detectar a ocorrência de anticorpos contra Leptospira spp., Brucella spp. eToxoplasma gondii em cães errantes Amostras de sangue colheram amostras de sangue de 181 cães errantes para a realização dos diagnósticos sorológicos. Das amostras analisadas, 36,46% foram reagentes para Tgondii, 16,57% para Leptospira spp. e9,39% para Brucella canis.A partir dos resultados obtidos no presente trabalho, verificou-se que essas zoonoses apresentam elevadaprevalência nos cães errantes do estudo. O elevado índice de infecção da população canina para estas zoonoses, torna-seum indicativo de que o ambiente está contaminado com diferentes micro-organismos representando um problema para a saúde única, uma vez que tanto o homem como o cão estão expostos a diferentes fontes de infecção.


Subject(s)
Animals , Dogs , Antibodies , Brucella canis/immunology , Brucellosis/veterinary , Leptospira interrogans serovar canicola/immunology , Leptospirosis , Toxoplasma/immunology , Toxoplasmosis, Animal , Zoonoses
4.
Semina Ci. agr. ; 36(3,supl.1): 1999-2004, 2015. mapas
Article in English | VETINDEX | ID: vti-26996

ABSTRACT

This study aimed to determine the prevalence of Toxoplasma gondii in swine slaughtered in Iporã, Northwest Paraná state. Blood samples were obtained from 500 finishing swine. All animals were raised under intensive farming; the blood samples were analyzed using the immunofluorescence antibody test (IFAT). Animals with titers of ?64 were considered positive for T. gondii infection. Of the tested samples, 63 (12.6%) were positive by IFAT; 58 (92.06%) of these showed titers of 64 (4.7%), with titers 256 and two (3.1%) titers of 1024. These pigs might be considered a source of T. gondii infection for humans.(AU)


O trabalho teve como objetivo determinar a soroprevalência de Toxoplasma gondii em suínos de um matadouro frigorífico em Iporã, região noroeste do Paraná. Amostras sanguíneas de 500 suínos de terminação, provenientes de granjas de criação intensiva, foram processadas utilizando-se a Reação de Imunofluorescência Indireta (RIFI). Os animais com título ? 64 foram considerados positivos. Dentre as amostras testadas, 63 (12,6%) foram positivas pela IFI. Sendo 58 (92,06%) com título 64 (4,7%) com título 256 e duas (3,1%) com titulação 1024. Estes suínos podem ser considerados uma fonte de infecção ao homem.(AU)


Subject(s)
Animals , Toxoplasma , Toxoplasmosis, Animal/epidemiology , Swine Diseases , Abattoirs
5.
Semina ciênc. agrar ; 36(3,supl.1): 1999-2004, 2015. map
Article in English | VETINDEX | ID: biblio-1500009

ABSTRACT

This study aimed to determine the prevalence of Toxoplasma gondii in swine slaughtered in Iporã, Northwest Paraná state. Blood samples were obtained from 500 finishing swine. All animals were raised under intensive farming; the blood samples were analyzed using the immunofluorescence antibody test (IFAT). Animals with titers of ?64 were considered positive for T. gondii infection. Of the tested samples, 63 (12.6%) were positive by IFAT; 58 (92.06%) of these showed titers of 64 (4.7%), with titers 256 and two (3.1%) titers of 1024. These pigs might be considered a source of T. gondii infection for humans.


O trabalho teve como objetivo determinar a soroprevalência de Toxoplasma gondii em suínos de um matadouro frigorífico em Iporã, região noroeste do Paraná. Amostras sanguíneas de 500 suínos de terminação, provenientes de granjas de criação intensiva, foram processadas utilizando-se a Reação de Imunofluorescência Indireta (RIFI). Os animais com título ? 64 foram considerados positivos. Dentre as amostras testadas, 63 (12,6%) foram positivas pela IFI. Sendo 58 (92,06%) com título 64 (4,7%) com título 256 e duas (3,1%) com titulação 1024. Estes suínos podem ser considerados uma fonte de infecção ao homem.


Subject(s)
Animals , Swine Diseases , Abattoirs , Toxoplasma , Toxoplasmosis, Animal/epidemiology
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