A Reliable Stem Cell Carrier: An Experimental Study in Wistar Rats.

INTRODUCTION: Treatments based on cell biology need reliable and precise carriers for reaching the desired targets. For that reason, a PDO-based cell carrier was idealized, with the purpose of carrying stem cells to distant sites at room temperature. MATERIALS AND METHODS: Three modalities of the same carrier were evaluated: one containing undifferentiated human dental pulp stem cells (DPSCs); one loaded with stem cells induced to neurogenic differentiation (DPSCNs); and one without cells (Blank). The carriers were implanted in sciatic nerve gaps in 48 Wistar rats that were divided in three groups. Two other rats were included in a SHAM control group. Immunohistochemical, histological and clinical analyses were performed in two, four, six and eight weeks of time. RESULTS: Efficacy of human stem cell transportation at room temperature to rats was attested. Moreover, it was possible to confirm that those cells show tropism for inflamed environments and are also prone to induction of neurogenesis in the first two weeks, vanishing after that period. CONCLUSION: Clinical evaluation of the animals' gait recovery shows a promising perspective of success with the inclusion of stem cell-loaded PDO tubes in nerve gaps, which may be positively compared to previously published studies. NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors - www.springer.com/00266.

Um novo instrumento para marcação intraoperatória / A new tool for intraoperative marking

Este artigo descreve a adaptação de um mandril de micro-punch - normalmente empregado em transplantes capilares - para uso em marcações intraoperatórias. O mandril foi usado como cabo para manuseio de palitos de dente esterilizados nas demarcações de procedimentos cirúrgicos diversos, como abdominoplastias, mamoplastias e ritidoplastias. O emprego deste instrumento oferece um meio confortável, acessível, preciso e de baixo custo para todos os tipos de marcações cirúrgicas em cirurgia plástica, sendo mais barato e eficiente do que canetas demarcatórias descartáveis.

This article describes the adaptation of a micro-punch mandrel, which is usually used in hair transplants, in intraoperative markings. The mandrel was used as a wire for sterile toothpick handling in different surgical marking procedures, such as those in abdominoplasties, mammoplasties, and rhytidectomies. The use of this instrument offers a comfortable, affordable, precise, and low-cost tool for all types of surgical marking performed in plastic surgery. It is cheaper and more efficient than disposable pens.

Mesenchymal stromal cells from adipose tissue attached to suture material enhance the closure of enterocutaneous fistulas in a rat model.

BACKGROUND AIMS: Surgical treatment for enterocutaneous fistulas (EF) frequently fails. Cell therapy may represent a new approach to treatment. Mesenchymal stromal cells (MSCs) have high proliferative and differentiation capacity. This study aimed to investigate whether MSCs could adhere to suture filament (SF), promoting better EF healing. METHODS: MSCs, 1 × 10(6), from adipose tissue (ATMSCs) were adhered to a Polyvicryl SF by adding a specific fibrin glue formulation. Adhesion was confirmed by confocal and scanning electron microscopy (SEM). A cecal fistula was created in 22 Wistar rats by incising the cecum and suturing the opening to the surgical wound subcutaneously with four separate stitches. The animals were randomly allocated to three groups: control (CG)-five animals, EF performed; injection (IG)-eight animals 1 × 10(6) ATMSCs injected around EF borders; and suture filament (SG): nine animals, sutured with 1 × 10(6) ATMSCs attached to the filaments with fibrin glue. Fistulas were photographed on the operation day and every 3 days until the 21st day and analyzed by two observers using ImageJ Software. RESULTS: Confocal and SEM results demonstrated ATMSCs adhered to SF (ATMSCs-SF). The average reduction size of the fistula area at 21st day was greater for the SG group (90.34%, P < 0.05) than the IG (71.80%) and CG (46.54%) groups. CONCLUSIONS: ATMSCs adhered to SF maintain viability and proliferative capacity. EF submitted to ATMSCs-SF procedure showed greater recovery and healing. This approach might be a new and effective tool for EF treatment.

Método de avaliação da suspensão do SMAS no rejuvenescimento facial / A method to evaluate the SMAS suspension in facelift

INTRODUÇÃO: A suspensão do sistema musculoaponeurótico superficial (SMAS) é um procedimento amplamente aceito nas ritidoplastias. Com o objetivo de avaliar a eficácia de tal suspensão na técnica de tratamento do SMAS em round block com cicatrizes curtas, foi proposto método em que a redução da área descolada é avaliada ao final da cirurgia, assegurando sua eficácia, baseada em clara evidência. MÉTODO: Foram analisadas 20 cirurgias consecutivas de ritidoplastia, todas realizadas pelo mesmo cirurgião, com os mesmos padrões. Foram tomadas 12 medidas em cada lado da face, 7 antes e 5 após a realização da suspensão, permitindo a elaboração de 40 diagramas compreendendo as áreas avaliadas antes e após o procedimento. Foi utilizado o software ImageJ (NIH Image) para cálculo das áreas. RESULTADOS: A área descolada nas 40 medidas apresentou média de 52,6 cm², mudando para 32,6 cm² após a suspensão, com redução média de 38%. Os diagramas obtidos refletiram a exata atuação nos diversos pontos da face. CONCLUSÕES: O método descrito confirma que a técnica é eficiente quanto à suspensão do SMAS, com redução significativa da área descolada e consequente diminuição do espaço morto pós-operatório, e pode servir como parâmetro para comparação de diferentes técnicas.

BACKGROUND: The superficial musculo-aponeurotic system (SMAS) suspension is a globally accepted procedure in rhytidoplasties. In order to evaluate the efficacy of suspension performed with the round block SMAS treatment, the authors planned a method in which the wound area reduction is measured to assure its functionality, based in clear evidence. METHODS: Twenty consecutive rhytidoplasties were evaluated, all of them done by the same surgeon, using the same standards. Twelve measures were taken in each side of the face, 7 before suspension and 5 after, allowing for the elaboration of 40 diagrams comprising the areas evaluated before and after suspension. The software ImageJ (NIH Image) was used to evaluate the surfaces. RESULTS: The undermined area in the 40 measures had a mean of 52.6 cm², which changed to a mean of 32.6 cm² after suspension, with a mean reduction of 38%. The diagrams also showed the exact handled points of the face. CONCLUSIONS: The described methodology confirms that the round block SMAS treatment technique is efficient regarding the suspension of the superficial muscular aponeurotic system, with significant reduction of the undermined area, consequently reducing the dead space, and may be a parameter to comparison of different techniques.

Stem cells from the fat tissue of rabbits: an easy-to-find experimental source.

Stem cells, because of their pluripotentiality and unlimited capacity for self-renewal, project great promise for tissue engineering and are expected to allow significant advances for distinct reconstructive procedures. This study aimed to establish a versatile experimental model with potential application to distinct lines of research in plastic surgery. The search was guided by the need for mesenchymal stem cells that can be easily obtained for laboratory research. The authors studied the anatomy of a number of animals as potential donors and indicated the white New Zealand rabbit (Oryctolagus cuniculus) for this purpose. The animals of this species present definite areas of lipid tissue on their back, allowing easy access to the tissue by either exeresis or liposuction.

Shortscar face-lift with the RoundBlock SMAS treatment: a younger face for all.

BACKGROUND: Performing a face-lift for smokers, bald people, and those looking for a quick recovery is always a challenge. The submucosal aponeurotic system (SMAS) plication technique with limited undermining provides great suspension and has proved to be a safe and fast way to perform a face-lift surgery. METHODS: The procedure is a suspension technique for face-lifting based on a purse-string suture around the ear that allows all the soft tissues of the face and neck to be elevated and anchored in the strong structures of the cranial muscles and aponeurosis. This allows a harmonic volumetric repositioning and results in almost no dead space because the circular plication diminishes the surgical undermining when stretched. A total of 225 face-lifts were performed. RESULTS: Because this procedure offers quick recovery and long-lasting results with minimal complications, patients become fearless about face-lift surgery. CONCLUSIONS: The RoundBlock SMAS treatment is a suspension method for face-lifts that is safe and fast, results in reduced scars and no dead space, requires no drains, and has a quick recovery time.