Subject(s)
Chromosome Mapping , Meat , Polymorphism, Genetic , Sus scrofa/genetics , Animals , Quantitative Trait LociSubject(s)
Calcium-Binding Proteins/genetics , Chromosome Mapping , Extracellular Matrix Proteins/genetics , GTP-Binding Proteins/genetics , Microfilament Proteins/genetics , Muscle, Skeletal/embryology , Osteonectin/genetics , Proteoglycans/genetics , Swine/genetics , Animals , Cell Adhesion Molecules/genetics , Decorin , Female , Fetus , Fibrillins , Gestational Age , Muscle, Skeletal/physiology , Pregnancy , CalponinsABSTRACT
The lamins are components of nuclear lamina and they have a profound influence on nuclear structure and functions. They are encoded by three genes, LMNA, LMNB1 and LMNB2. A genomic fragment of the porcine LMNA gene (822 bp; from exons 7 to 9) was amplified by polymerase chain reaction and comparatively sequenced. Four single nucleotide polymorphisms (SNPs) were identified in intronic sequences: G162A, G208A, T367G and C618T. The SNPs are within the restriction sites for enzymes Bsh1236I, HpaII, AluI and Bsh1236I respectively. Allele frequencies at SNPs G208A, T367G and C618T were determined by using eight pig breeds. Linkage analysis in the Hohenheim Meishan x Piétrain family placed the LMNA gene in the chromosome 4q linkage group, between MEF2D and GBA (MEF2D - 3.0 cM - LMNA - 0.2 cM - GBA). In radiation hybrid mapping LMNA was most significantly linked to SW270 on chromosome 4 (39 cR; LOD = 7.86). The LMNA gene is located in the quantitative trait loci region for some carcass traits on chromosome 4q.
Subject(s)
Chromosomes, Mammalian/genetics , Genetic Linkage/genetics , Lamin Type A/genetics , Polymorphism, Single Nucleotide/genetics , Radiation Hybrid Mapping/veterinary , Swine/genetics , Alleles , AnimalsABSTRACT
The porcine orthologues of human chromosome HSA9q22.31 genes osteoglycin (OGN) and asporin (ASPN) were mapped to porcine chromosome SSC3 using linkage analysis and a somatic cell hybrid panel. This mapping was refined to SSC3q11 using fluorescence in situ hybridization. These results confirm the existence of a small conserved synteny group between SSC3 and HSA9. Polymorphisms were revealed in both genes, including a pentanucleotide microsatellite (SCZ003) in OGN and two single nucleotide polymorphisms (AM181682.1:g.780G>T and AM181682.1:g.825T>C) in ASPN. The two genes were included in a set of markers for quantitative trait loci (QTL) mapping on SSC3 in the Hohenheim Meishan x Piétrain F2 family. Major QTL for growth and carcass traits were centred in the ASPN-SW902 region.