Characterizing leaf-deposited particles: Single-particle mass spectral analysis and comparison with naturally fallen particles.

The size and composition of particulate matter (PM) are pivotal in determining its adverse health effects. It is important to understand PM's retention by plants to facilitate its atmospheric removal. However, the distinctions between the size and composition of naturally fallen PM (NFPM) and leaf-deposited PM (LDPM) are not well-documented. Here we utilize a single-particle aerosol mass spectrometer, coupled with a PM resuspension chamber, to analyze these differences. We find that LDPM particles are 6.8-97.3 % larger than NFPM. Employing a neural network algorithm based on adaptive resonance theory, we have identified distinct compositional profiles: NFPM predominantly consists of organic carbon (OC; 31.2 %) and potassium-rich components (19.1 %), whereas LDPM are largely composed of crustal species (53.9-60.6 %). Interestingly, coniferous species retain higher OC content (11.5-13.7 %) compared to broad-leaved species (0.5-1.2 %), while the levoglucosan content exhibit an opposite trend. Our study highlights the active role of tree leaves in modifying PM composition beyond mere passive capture, advocating for a strategic approach to species selection in urban greening initiatives to enhance PM mitigation. These insights provide guidance for urban planners and environmentalists in implementing nature-based solutions to improve urban air quality.

Uneven PM2.5 dispersion pattern across an open-road vegetation barrier: Effects of planting combination and wind condition.

The increased traffic-induced emissions contribute to the exacerbation of airborne particulate matter (PM) pollution. The vegetation barrier (VB) provides a means of reducing the traffic-induced pollutants. However, the effects of VB configuration and local environment on PM dispersion and reduction remain unclear, and thereby needs further advancement on VB design and characteristics. This study constructed a 3D numerical model based on field survey in an open-road VB of Shanghai urban area, and then simulated PM2.5 dispersion under various VB configurations and wind conditions. The results consolidated that the presence of the VB reduced PM2.5 concentration by over 15 % across the VB. A greater bush coverage (2/3 and more) reduces over 14 % more PM2.5 pollution across the VB than that for a greater arbor coverage, and reduces 6 % more PM2.5 pollution in the sidewalk canyon. Given a certain bush planting coverage, planting bushes in the windward area is beneficial to the overall PM2.5 reduction by approximately 4-14 %. The wind directions determine the overall pattern of PM2.5 dispersion across the VB plot, decreasing trends for perpendicular winds but fluctuating curves for parallel winds Wind velocities largely contribute to the changing rates of PM2.5 concentration, the increased wind speed from 1 m/s to 7 m/s accumulated 5-11 % more PM2.5 pollution across the VB plot. This study provides practical insights for effective VB designs in order to mitigate the PM pollution and the human's exposure to PM2.5 in urban open-road environments.

Decoupling between carbon source and sink induced by responses of daily stem growth to water availability in subtropical urban forests.

Urban forests are anticipated to offer sustainable ecosystem services, necessitating a comprehensive understanding of the ways in which trees respond to environmental changes. This study monitored stem radius fluctuations in Cinnamomum camphora and Taxodium distichum var. imbricatum trees using high-resolution dendrometers at two sites, respectively. Gross primary production (GPP) was measured using eddy-covariance techniques and aggregated to daily sums. Hourly and daily stem radius fluctuations were estimated across both species, and the responses of stems to radiation (Rg), air temperature (Tair), vapor pressure deficit (VPD), and soil humidity (SoilH) were quantified using Bayesian linear models. The diel growth patterns of the monitored trees showed similar characteristics at the species level. Results revealed that trees growth occurred primarily at night, with the lowest hourly contribution to total growth and probability for growth occurring in the afternoon. Furthermore, the Bayesian models indicated that VPD was the most important driver of daily growth and growth probability. After considering the potential constraints imposed by VPD, a modified Gompertz equation showed good performance, with R2 ranging from 0.94 to 0.99 for the relationship between accumulative growth and time. Bayes-based model-independent data assimilation using advanced Markov chain Monte Carlo (MCMC) algorithms provided deeper insights into nonlinear model parameterization. Finally, the quantified relationship between GPP and stem daily growth revealed that the decoupling between carbon source and sink increased with VPD. These findings provided direct empirical evidence for VPD as a key driver of daily growth patterns and raise questions about carbon neutrality accounting under future climate change given the uncertainties induced by increased water stress limitations on carbon utilization.

Phosphorus elevation erodes ectomycorrhizal community diversity and induces divergence of saprophytic community composition between vegetation types.

Phosphorus (P) is a critical macronutrient that is essential for many life-sustaining processes. Despite decades of work on plant performance under P deficiency and the importance of microbes in ecosystem processes, little is known about how bacterial and fungal flora respond to P gradients and determine the vegetation health. In current study, we examined soil edaphic conditions and microbial communities in 39 untouched natural forests representing phosphorous deficient (Pp) and phosphorus rich (Pr) soils (due to naturally occurring phosphate rocks) in Yunnan Province, China. We also considered the effect of plant functional types by including the dominant tree species. Bacterial and fungal diversity was greater across the Pp sites compared with Pr sites. The relative abundance of Actinobacteria and Gemmatimonadetes was higher across Pp sites, while Chlamydiae and Verrucomicrobia showed the opposite pattern, with greater relative abundance across the Pr sites. Bacterial taxa that were observed in low P soils were more likely having oligotrophic life history strategies. Interestingly, ectomycorrhizal (ECM) fungal diversity was promoted in the Pp sites, indicating that the decreasing soil P concentration and the increasing host P demand foster stimulated the ECM species for hyphal soil exploration. Moreover, the high P level caused saprophytic fungi (SAP) to diverge, causing its enrichment only under Q. variabilis compared to low P soil, where there is no difference in relative abundance of SAP between the two tree species. This likely resulted in an enhanced decomposition process by SAP and elevation of soil properties (Carbon and Nitrogen) under Q. variabilis across the Pr sites. Taken together, our findings highlight the highly diverse microbiome in low P soils. The higher soil P caused shifts of fungal functional guilds, which likely influence tree growth and health (ECM), along with divergence of ecosystem services between tree functional types.

Photosynthesis and Related Physiological Parameters Differences Affected the Isoprene Emission Rate among 10 Typical Tree Species in Subtropical Metropolises.

Volatile organic compound (VOCs) emission is an important cause of photochemical smog and particulate pollution in urban areas, and urban vegetation has been presented as an important source. Different tree species have different emission levels, so adjusting greening species collocation is an effective way to control biogenic VOC pollution. However, there is a lack of measurements of tree species emission in subtropical metropolises, and the factors influencing the species-specific differences need to be further clarified. This study applied an in situ method to investigate the isoprene emission rates of 10 typical tree species in subtropical metropolises. Photosynthesis and related parameters including photosynthetic rate, intercellular CO2 concentration, stomatal conductance, and transpiration rate, which can influence the emission rate of a single species, were also measured. Results showed Salix babylonica always exhibited a high emission level, whereas Elaeocarpus decipiens and Ligustrum lucidum maintained a low level throughout the year. Differences in photosynthetic rate and stomatal CO2 conductance are the key parameters related to isoprene emission among different plants. Through the establishment of emission inventory and determination of key photosynthetic parameters, the results provide a reference for the selection of urban greening species, as well as seasonal pollution control, and help to alleviate VOC pollution caused by urban forests.

Individual effects of trichomes and leaf morphology on PM2.5 dry deposition velocity: A variable-control approach using species from the same family or genus.

Urban green infrastructure is closely linked to the alleviation of pollution from atmospheric particulate matter. Although particle deposition has been shown to depend on leaf characteristics, the findings from earlier studies are sometimes ambiguous due to the lack of controlling variables. In this study, we investigated the impact of leaf morphological characteristics on PM2.5 dry deposition velocity by employing a control-variable approach. We focused on four indices: trichome density, petiole length, aspect ratio (width-to-length ratio), and fractal deviation. For each index, tree species were chosen from the same family or genus to minimize the influence of other factors and make a group of treatments for an individual index. The dry deposition velocities of PM2.5 were determined through application of an indirect method. The results revealed that the presence of leaf trichomes had a positive effect on PM2.5 dry deposition velocity, and a higher trichome density also led to a greater particle deposition velocity. Lower leaf aspect ratio, shorter petioles, and higher leaf fractal deviation were associated with greater PM2.5 dry deposition velocity. The control-variable approach allows to investigate the correlation between deposition velocity and a certain leaf characteristic independently while minimizing the effects of others. Thus, our study can clarify how a single leaf characteristic affects particle deposition velocity, and expound its potential mechanism more scientifically than the published studies. Our research points out the importance of controlling variables, and also provides ideas for future researches on related factors to be found. Meanwhile the results would help provide insight into design improvements or adaptive management for the alleviation of air pollution.

Bacterial Communities Are More Sensitive to Water Addition Than Fungal Communities Due to Higher Soil K and Na in a Degraded Karst Ecosystem of Southwestern China.

Precipitation is predicted to become more intense in Southern China in the context of climate change; however, the responses of microbial communities to variations in soil moisture have not been well documented for karst areas. The climate is typically in a subtropical monsoon category with two different seasons: a dry season (December-May) and a wet season (June-November). Based on a randomized complete block design (RCBD), a water addition experiment (0, +20, +40, and +60% relative to local precipitation) was established in April 2017, with five replicates, in a degraded grass-shrub community. Sampling was performed in May and at the end of August of 2017. Macroelements (C, H, N, P, K, Ca, Mg, and S), microelements (Mn, Fe, Zn, and Cu), and non-essential elements (Na, Al, and Si) were quantified in the soil. The total DNA of the soil samples was analyzed through 16S rRNA amplicon by Illumina Miseq. Subsequent to the addition of water during both the dry and wet seasons, the concentrations of non-metal elements (C, H, N, S, and P, except for Si) in the soil remained relatively stable; however, metal elements (K, Na, Fe, and Mg, along with Si) increased significantly, whereas Zn and Ca decreased. During the dry season, fungal and bacterial communities were significantly distinct from those during the wet season along the PC axis 1 (p < 0.001). Water addition did not alter the compositions of bacterial or fungal communities during the dry season. However, during the wet season, water addition altered the compositions of bacterial rather than fungal community based on principal component analysis. At the phylum level, the relative abundance of Actinobacteria increased with water addition and had a significantly positive correlation with K+ (r 2 = 0.70, p < 0.001) and Na+ (r 2 = 0.36, p < 0.01) contents, whereas that of Acidobacteria, Planctomycetes, and Verrucomicrobia decreased and showed negative correlation with soil K and Na content, and no changes were observed for the fungal phyla. This suggests that the karst bacterial communities can be influenced by the addition of water during the wet season likely linked to changes in soil K and Na contents. These findings implied that increased rainfall might alter the elemental compositions of karst soils, and bacterial communities are likely to be more sensitive to variations in soil moisture in contrast to their fungal counterparts.

Roles of metabolic regulation in developing Quercus variabilis acorns at contrasting geologically-derived phosphorus sites in subtropical China.

BACKGROUND: Phosphorus (P) -rich soils develop in phosphorite residing areas while P-deficient soils are ubiquitous in subtropical regions. Little has been reported that how metabolites participate in the seed development and the processes involved in their coping with contrasting-nutrient environments. RESULTS: Here we quantified the metabolites of Quercus variabilis acorns in the early (July), middle (August), late (September) development stages, and determined element (C, H, O, N, P, K, Ca, Mg, S, Fe, Al, Mn, Na, Zn, and Cu) concentrations of acorns in the late stage, at geologically-derived contrasting-P sites in subtropical China. The primary metabolic pathways included sugar metabolism, the TCA cycle, and amino acid metabolism. Most metabolites (especially C- and N-containing metabolites) increased and then decreased from July to September. Acorns between the two sites were significantly discriminated at the three stages, respectively, by metabolites (predominantly sugars and organic acids). Concentrations of P, orthophosphoric acid and most sugars were higher; erythrose was lower in late-stage acorns at P-rich sites than those at P-deficient sites. No significant differences existed in the size and dry mass of individual acorns between oak populations at the two sites. CONCLUSIONS: Oak acorns at the two sites formed distinct metabolic phenotypes related to their distinct geologically-derived soil conditions, and the late-stage acorns tended to increase P-use-efficiency in the material synthesis process at P-deficient sites, relative to those at P-rich sites.

Effects of the leaf functional traits of coniferous and broadleaved trees in subtropical monsoon regions on PM2.5 dry deposition velocities.

Plants can intercept airborne particulate matter through deposition. Different types of plants exhibit different functional leaf traits, which can affect the dry deposition velocity (Vd). However, the most crucial leaf traits of coniferous and broadleaved trees remain unidentified. In this study, we selected 18 typical plants from the subtropical monsoon regions, where PM2.5 (fine particulate matter with a diameter of ≤2.5 µm) concentrations are relatively high, and classified them into coniferous and broadleaved categories. Subsequently, we analyzed the relationships between Vd and leaf surface free energy (SFE), single leaf area (LAs), surface roughness (SR), specific leaf area (SLA), epicuticular wax content (EWC), and width-to-length ratio (W/L). The results indicated that most coniferous trees exhibited a high Vd. The correlation analysis revealed that SFE, SR, LAs, and W/L were the key factors that affected the Vd of all the tested species. SFE and SLA had the strongest influence on the Vd of broadleaved trees, whereas LAs and SLA had the strongest effect on that of coniferous trees. Most coniferous trees had a high SLA, which can reduce water loss and hinder particle deposition. However, the stiff leaves of coniferous trees fluttered less, resulting in a larger leaf area that enhanced the capture efficiency. The leaf structure of broadleaved trees is more flexible, resulting in erratic flutter, which may impede deposition and lead to high resuspension. Coniferous and broadleaved trees may have different dominant leaf traits that affect particle deposition.

Polycyclic aromatic hydrocarbons in leaves of Cinnamomum camphora along the urban-rural gradient of a megacity: Distribution varies in concentration and potential toxicity.

Rapid urbanization and industrialization have precipitated the significant urban-rural gradient involving various aspects of human-related activities especially in megacities. Anthropogenic activities are the main source of polycyclic aromatic hydrocarbon (PAH) contamination, and the rising awareness concerning PAH potential toxicity to human health promotes a further understanding of its spatial distribution pattern in cities. Whether the distribution of PAH concentration and potential toxicity respond to the urban-rural gradient still requires investigation. This study applied a grid sampling method to investigate PAH concentration using Cinnamomum camphora leaves as bioindicators which were obtained from 84 sampling sites in a megacity, Shanghai. The potential toxicity of PAHs in leaves was calculated by toxicity factor equivalent method. Results revealed the patterns of PAH distribution in the city varied in concentration and potential toxicity: the total concentration of PAHs in leaves decreased along the urban-rural gradient, while the potential toxicity peaked at junction areas. The trend of PAH concentration along the distance from urban center corresponded to that of population density. The spatial distribution of potential toxicity did not correspond with the gradient but was influenced by high benzo(a)pyrene concentration originated from the industry districts nearby. Higher potential toxicity of PAHs was observed at the urban-suburban-rural junction areas of megacities, advocating health-risk attention and appropriate plan for land use of these transition areas in cities.

Coagulation effect of aero submicron particles on plant leaves: Measuring methods and potential mechanisms.

Aero submicron particles (d < 1 µm) have attracted widely attention due to their difficulty in removal from the air and serious threat to human health. Leaves are considered as important organs to purify particulate matter and alleviate air pollution. However, the current research mainly focuses on the removal capacity of particulate matter by urban plants at different scales, there are relatively few studies on the change of particle diameter at the air-leaf interface during this process. This study is one of the first to propose the existence of coagulation effect of aero submicron particles on the leaves, and a sweep-resuspension method and X-ray microscope were used to measure such size changes of two typical subtropical broad-leaf plants. The results showed that the size of submicron particles increased significantly during the migration from atmosphere to leaf surface: the average particle size increased from 0.48 µm at emission to 3.40 µm on the leaf surface, while the proportion of submicron particles decreased from 95% to less than 20%. The sweep-resuspension method was easy to implement, the data was easy to obtain, and the cost was low, therefore it could be widely used in the determination of the coagulation effect. The coagulation effect was also inferred as an important mechanism used by plants to reduce particulate matter. In the process of particulate removal: coagulation effect and dry deposition are actually two steps that occur simultaneously and interact. This finding refined the understanding of particulate removal processing, and laid a foundation for further research on factors affecting coagulation, which can be helpful for optimizing tree species selection and plant arrangement.

Differential stoichiometric responses of shrubs and grasses to increased precipitation in a degraded karst ecosystem in Southwestern China.

The elemental concentrations of both plants and soils are sensitive to variations in precipitation due to the limiting roles of water on soil processes and plant growth in karst ecosystems of Southwestern China; however, precipitation is predicted to increase in this region. Nevertheless, it is unclear how the elemental composition of soils and plants might respond to such increases in moisture. Particularly, how potassium (K) may behave as a key mediator in the regulation of the water potential of plants. For this study, the responses of the elemental composition of both soils and plants to the variable addition of water were investigated. Two grasses (Cymbopogon distans and Arundinella setosa) and two shrubs (Carissa spinarum and Bauhinia brachycarpa) were investigated under four levels of watering treatments 0%, +20%, +40%, and +60%, relative to the annual rainfall, respectively. Compared to the control (CK), the soil water content (SWC) increased to 3.75, 3.86, and 4.34 mg g-1 in T1, T2, and T3 groups, respectively (p < 0.05). Non-metal elements (C, H, N, S, and P, except for Si) in the soil were relatively stable with water addition; however, metal elements (Al, Na, Mg, Fe, and K, along with Si) increased significantly, whereas Zn and Ca decreased (p < 0.05). With water addition, leaf N and P remained unchanged in all four species, while K, Mg, and S decreased in both shrubs (higher C:K, N:K, and P:K). Increases in Fe, Si, and K were observed in both grasses (lower C:K, N:K, and P:K), which suggested that K played distinct roles for water regulation in shrubs and grasses. These findings implied that the elemental compositions of both soils and plants might be altered with increasing precipitation in the future, where different plant types may adopt distinct K-regulation strategies to cope with variable soil moisture.

Correlated metabolic and elemental variations between the leaves and seeds of oak trees at contrasting geologically derived phosphorus sites.

The leaves and seeds of plants frequently function as the source and sink organs for distinct metabolites, which can interactively vary in response to adverse site conditions. Subtropical soils are typically characterized as having deficient phosphorus (P), calcium (Ca), and magnesium (Mg), with enriched aluminum (Al) and iron (Fe), while Al and manganese (Mn) are toxic at low pH. It remains largely unknown how leaf- and seed-sourced metabolites are synergistically linked to adapt to P-variable soils for trees in subtropical areas. Here we quantified the metabolic and elemental profiling in the mature leaves and immature seeds of Quercus variabilis at contrasting geologically-derived phosphorus sites in subtropical China. The results revealed that carbon (C) and nitrogen (N) based metabolites (primarily sugars and organic acids), as well as enzyme- and protein/nucleic acid-related elements (N, P, Mg, and Mn) played important roles toward characterizing the profiling of metabolites and ionomes in leaves and seeds at two site types, respectively. These metabolites (sugars, amino acids, and fatty acids) and elements (N, P, Mg, and Mn) of seeds were closely related to the sugars, organic acids, and elements (N, P, Mg, and Mn) of leaves at the two site types. For the most part, the content of N and P in the soil affected the accumulation of materials (such as, starchs and proteins) in seeds, as well as N and P assimilation in leaves, by influencing C- and N-containing metabolites in leaves. These results suggested that correlated disparities of C- and N-containing metabolites, along with enzyme- and protein/nucleic acid-related elements in both leaves and seeds played important roles in plants to facilitate their adaptation to nutrient-variable sites in subtropical zones.

Differential metabolic responses of shrubs and grasses to water additions in arid karst region, southwestern China.

Increasing precipitation has been predicted to occur in the karst areas in southwestern regions of China. However, it is little known how various plants respond to increasing precipitation in this region. Here we determined the impacts of water addition on leaf metabolites of grasses (Cymbopogon distans and Arundinella sitosa) and shrubs (Carissa spinarum and Bauhinia brachycarpa) in this area. Four levels of water additions (CK, T1, T2 and T3 indicating 0%, +20%, +40% and +60% relative to the current monthly precipitation, respectively) were designed. Sphingolipids substantially increased in the leaves of all four species with increasing water supply which suggests that these plants adopted biochemical strategy to tolerate the wet stress. However, both shrubs showed decreases in valine and threonine (amino acids), threonate, succinate and ascorbic acid (organic acids), galactose and rhamnose (sugars) and epicatchin and oleamides (secondary metabolites) with increasing water supply. Both grasses increased in the total metabolites at T1, but the total metabolites in A. sitosa significantly decreased at T2 and T3 while remains unchanged in C. distans. Tri-carboxylic acid cycle and amino acid metabolism in shrubs and shikimate pathway in grasses were strongly affected with water supply. Overall, shrubs and grasses respond differentially to variation in water addition in terms of metabolomics, which is helpful in understanding how plants respond to climate change.