ABSTRACT
Ultrastructural characteristics of vegetative and zoosporangial stages of cultured Perkinsus marinus, a pathogen of the eastern oyster, Crassostrea virginica, were examined by transmission electron microscopy. An axenic cell culture was propagated from infected Chesapeake Bay oyster hemolymph. Different stages of the in vitro cell cycle, including schizonts and different size trophonts, were examined. Trophonts had spherical nuclei with wide perinuclear spaces, mitochondria with tubular cristae, and vacuoles with vacuoplasts. There were micropores on the inside of cell walls. A tubular network in the cytoplasm connected lomasomes to vacuoles, and contained vacuoplast precursor material. Vacuoplasts and precursor material diminished when cell cultures were not fed, suggesting a function in metabolite storage. Cells divided by schizogony or binary fission. Daughter cells in a schizont were not alike, and may specialize for different functions. Some of the daughter cells in a schizont died. Some hypnospores, directly isolated from infected oyster hemolymph enlarged in Ray's fluid thioglycollate medium, and were induced to zoosporulate. Zoosporangia contained varicose, hypha-like structures, whose apical tips gave rise to prezoospores. Ultrastructural characteristics of the vegetative and zoosporangial stages did not resemble any apicomplexan parasites other than members of the genus Perkinsus.
Subject(s)
Apicomplexa/growth & development , Ostreidae/parasitology , Animals , Apicomplexa/ultrastructure , Cell Cycle/physiology , Microscopy, ElectronABSTRACT
The haplosporidian oyster parasite MSX (Multinucleated Sphere X) Haplosporidium nelsoni was transmitted to eastern oysters Crassostrea virginica. Hatchery-raised, MSX-free juvenile oysters were placed in upweller tanks. Water to the tanks was filtered through a screen with 1 mm2 openings and originated from the water column overlaying naturally infected oysters beds (MSX prevalence 17 to 57%). MSX was diagnosed by histopathological analysis. MSX-disease (57% prevalence) with increased mortality (19%) was observed 11 wk after the beginning of the exposure and mortality of 80% after 16 wk. The study demonstrates transmission of MSX via water-borne infectious agents capable of passing through a 1 mm filter.
Subject(s)
Aquaculture/methods , Eukaryota/physiology , Ostreidae/parasitology , Animals , ConnecticutABSTRACT
Persistent generalized lymphadenopathy often develops during HIV-infection. It is characterized by follicular hyperplasia which progresses over time to follicular involution and finally lymphocyte depletion. To determine whether activated cytotoxic T cells (CD8+) are present in the hyperplastic germinal centres, light and electronmicroscopic immunogold labelling with monoclonal antibodies were used to localize two cytotoxic molecules, perforin and TIA-1. Perforin and TIA-1-positive cells were detected in the follicles and paracortex of lymph nodes from HIV-infected patients, whereas labelling was seen only in cells of the paracortex in the hyperplastic lymph nodes from HIV-negative patients. Cytotoxic granules, staining positive for perforin and TIA-1, were identified by transmission electronmicroscopy, often in proximity to follicular dendritic cells within the hyperplastic germinal centres of only HIV-positive patients. These cytotoxic cells may play a role in the follicular dendritic cell loss and concomitant follicular involution that occur during the evolution of HIV disease.
Subject(s)
HIV Infections/immunology , HIV Infections/pathology , HIV-1/immunology , Lymph Nodes/pathology , Lymph Nodes/ultrastructure , Lymphocyte Activation , Proteins , T-Lymphocytes, Cytotoxic/immunology , T-Lymphocytes, Cytotoxic/ultrastructure , Adult , Aged , Female , Granulocyte Colony-Stimulating Factor/analysis , HIV Infections/metabolism , Humans , Lymph Nodes/chemistry , Male , Membrane Glycoproteins/analysis , Membrane Proteins/analysis , Microscopy, Immunoelectron , Middle Aged , Perforin , Poly(A)-Binding Proteins , Pore Forming Cytotoxic Proteins , RNA-Binding Proteins/analysis , T-Cell Intracellular Antigen-1ABSTRACT
OBJECTIVE: To study whether free gp120 can be detected on the plasma membranes of apoptotic CD4+ T lymphocytes in lymph nodes from HIV-positive patients. METHODS: Lymph-node cell suspensions prepared from three HIV-positive patients were studied by pre-embedding, double-immunogold-labeling to identify cell type, determine cell morphology, and detect the presence of bound gp120 molecules. Cells were classified by their surface antigens as helper/inducer T lymphocytes (CD4+), cytotoxic/suppressor T lymphocytes (CD8+), B cells (CD20+), and total lymphocytes [CD45+, leukocyte common antigen (LCA)+]. RESULTS: gp120 colabelled with both apoptotic and normal CD4+ T lymphocytes and LCA+ cells, but not with either apoptotic or normal CD8+ T lymphocytes or B cells. gp120 was more often identified on apoptotic than on normal CD4+ T lymphocytes. The gp120 and CD45 label were often colocalized. HIV particles were not identified to be associated with or budding from either normal or apoptotic lymphocytes. CONCLUSIONS: Free gp120 is found bound to CD4+ T cells in lymph nodes of HIV-infected individuals and potentially mark them for premature death by apoptosis.
Subject(s)
CD4-Positive T-Lymphocytes/virology , HIV Envelope Protein gp120/analysis , HIV Infections/immunology , HIV-1 , Lymph Nodes/pathology , Apoptosis , Cell Membrane/virology , HIV Infections/virology , Humans , Immunophenotyping , Leukocyte Common Antigens/analysis , Lymph Nodes/immunology , Microscopy, ImmunoelectronABSTRACT
Despite intensive investigation, no clearly defined mechanism explaining human immunodeficiency virus (HIV)-induced cell killing has emerged. HIV-1 infection is initiated through a high-affinity interaction between the HIV-1 external envelope glycoprotein (gp120) and the CD4 receptor on T cells. Cell killing is a later event intimately linked by in vitro genetic analyses with the fusogenic properties of the HIV envelope glycoprotein gp120 and transmembrane glycoprotein gp41. In this report, we describe aberrancies in cell cycle regulatory proteins initiated by cell-cell contact between T cells expressing HIV-1 envelope glycoproteins and other T cells expressing CD4 receptors. Cells rapidly accumulate cyclin B protein and tyrosine-hyperphosphorylated p34cdc2 (cdk1) kinase, indicative of cell cycle arrest at G2 phase. Moreover, these cells continue to synthesize cyclin B protein, enlarge and display an abnormal ballooned morphology, and disappear from the cultures in a pattern previously described for cytotoxicity induced by DNA synthesis (S phase) inhibitors. Similar changes are observed in peripheral blood mononuclear cells infected in vitro with pathogenic primary isolates of HIV-1.
Subject(s)
Apoptosis , G2 Phase , HIV Envelope Protein gp120 , HIV Infections/pathology , HIV-1 , T-Lymphocytes/pathology , CD4 Antigens/metabolism , CD4-Positive T-Lymphocytes/immunology , CDC2 Protein Kinase/metabolism , Cell Communication , Cell Separation/methods , Cyclins/metabolism , Cytopathogenic Effect, Viral , Fluorescent Antibody Technique , HIV-1/isolation & purification , Humans , Leukocytes, Mononuclear/pathology , Phosphorylation , T-Lymphocytes/immunologyABSTRACT
1. Serum proteins from sarcomatous soft shell clams, Mya arenaria L., enhanced transmission of sarcoma. 2. Sarcoma cells were isolated and administered to the recipients at the same cell density in different sarcoma-protein-free diluents: seawater, serum from normal clams, heat-treated sarcoma serum or protease-digested sarcoma serum. 3. Transmission in these groups was significantly slower than in the group where cells were administered in intact sarcoma serum, demonstrating that the tumor promoting factors in the serum were heat-sensitive proteins. 4. Normal hemocytes administered in sarcoma serum caused mortality but not sarcoma transmission, suggesting the presence of cytotoxic factors in sarcoma serum.
Subject(s)
Bivalvia , Blood Proteins/analysis , Carcinogens/analysis , Sarcoma, Experimental/blood , Animals , Bivalvia/chemistry , Hemolymph/chemistry , KineticsABSTRACT
1. Serum proteins showed quantitative and qualitative differences between sarcomatous and healthy soft shell clams, Mya arenaria L. 2. Total protein concentration was significantly higher in the serum of sarcomatous clams than of healthy clams. 3. According to SDS-PAGE, more serum proteins with more variability distinguished sarcomatous clams from healthy ones. 4. Sarcomatous clams had unique serum proteins of M(r)23,000, 45,000 and 54,000. Healthy clams had unique serum proteins of M(r) 24,000, 103,000 and 105,000. 5. During disease progression, sarcoma-specific proteins appeared while normal proteins disappeared. 6. We propose that some sarcoma-associated proteins may have tumor promoting and/or cytotoxic functions and that some normal proteins which disappear during disease progression may be involved in the humoral defense system.