The evolution of structural characteristics and redox properties of humin during the composting of sludge and corn straw.

Humins (HMs), the insoluble faction of humic substances (HSs), play a pivotal role in the bioremediation of pollutants by acting as electron shuttles that modulate the interactions between microorganisms and pollutants. This crucial function is intricately linked to their structural composition and electron transfer capabilities. However, the dynamics of the electron transfer capacity (ETC) of HM extracted during the composting process and its determinants have yet to be fully elucidated. This study undertakes a comprehensive analysis of the ETC of HM derived from composting, employing electrochemical techniques alongside spectroscopic methods and elemental analysis to explore the influencing factors, including the electron accepting capacity (EAC), electron donating capacity (EDC), and electron reversible rate (ERR). Our findings reveal substantial variations in the EAC and EDC of HM throughout the composting process, with EAC values ranging from 133.03-220.98 µmol e- gC-1 and EDC values from 111.17-229.33 µmol e- gC-1. Notably, the composting process enhances the ERR and EDC of HM while diminishing their EAC. This shift is accompanied by an augmented presence of aromatic structures, polar functional groups, quinones, and nitrogen - and sulfur-containing moieties, thereby boosting the HM's EDC. Conversely, the reduction in EAC is associated with a decline in lignin carbon content and the abundance of oxygen-containing moieties, as well as the diminishment of visible fulvic-like and protein-like substances within HM. Importantly, humic-like substances and nitrogen-containing moieties within HM demonstrated the capacity for repeated electron transfer, underscoring their significance in the context of environmental remediation.

The art of fixing a ticking time bomb: Combined phacoemulsification and amniotic membrane transplantation.

Bleb leakage is a notorious complication of glaucoma filtration surgery which increases the risk of sight-threatening conditions. A 25-year-old female with severe bilateral juvenile open-angle glaucoma was treated for blebitis and exogenous endophthalmitis secondary to chronic bleb leak after undergoing XEN implantation, followed by multiple rounds of bleb needling, and augmented trabeculectomy. In the right eye, visual acuity was hand movement with cataract, intraocular pressure was 6 mmHg and the bleb was large, highly elevated from 10 to 1 o'clock, avascular, thin wall, and cystic with leaking points. Combined surgery of low-setting phacoemulsification and amniotic membrane transplantation without excising and manipulating the bleb was performed in the same setting. At postoperative 1 month, 6 months, and 1 year, her right vision had improved to 6/24, and the intraocular pressure was 12-14 mmHg, and the bleb leakage had resolved. This successful treatment was accomplished by maintaining the bleb's viability, preventing additional injury, and promoting wound healing.

Application value of multi-gene mutation detection in the clinical management of pediatric papillary thyroid carcinoma: a preliminary exploration.

Objectives: Thyroid cancer rarely occurs in children and adolescents. Molecular markers such as BRAF, RAS, and RET/PTC have been widely used in adult PTC. It is currently unclear whether these molecular markers have equivalent potential for application in pediatric patients. This study aims to explore the potential utility of a multi-gene conjoint analysis based on next-generation targeted sequencing for pediatric papillary thyroid carcinoma (PTC). Materials and methods: The patients diagnosed with PTC (aged 18 years or younger) in the pediatrics department of Lishui District Hospital of Traditional Chinese Medicine were retrospectively screened. A targeted enrichment and sequencing analysis of 116 genes associated with thyroid cancer was performed on paraffin-embedded tumor tissues and paired paracancerous tissue of fifteen children (average age 14.60) and nine adults (average age 49.33) PTC patients. Demographic information, clinical indicators, ultrasonic imaging information and pathological data were collected. The Kendall correlation test was used to establish a correlation between molecular variations and clinical characteristics in pediatric patients. Results: A sample of 15 pediatric PTCs revealed a detection rate of 73.33% (11/15) for driver gene mutations BRAF V600E and RET fusion. Compared to adult PTCs, the genetic mutation landscape of pediatric PTCs was more complex. Six mutant genes overlap between the two groups, and an additional seventeen unique mutant genes were identified only in pediatric PTCs. There was only one unique mutant gene in adult PTCs. The tumor diameter of pediatric PTCs tended to be less than 4cm (p<0.001), and the number of lymph node metastases was more than five (p<0.001). Mutations in specific genes unique to pediatric PTCs may contribute to the onset and progression of the disease by adversely affecting hormone synthesis, secretion, and action mechanisms, as well as the functioning of thyroid hormone signaling pathways. But, additional experiments are required to validate this hypothesis. Conclusion: BRAF V600E mutation and RET fusion are involved in the occurrence and development of adolescent PTC. For pediatric thyroid nodules that cannot be determined as benign or malignant by fine needle aspiration biopsy, multiple gene combination testing can provide a reference for personalized diagnosis and treatment by clinical physicians.

Identification of a novel genomic variance of BRAF1 in papillary thyroid carcinoma: A case report.

RATIONALE: Papillary thyroid carcinoma (PTC), the predominant subtypes accounting for approximately 85% of thyroid carcinomas, has a rapidly increasing global incidence rate. Statistically, approximately 74.6% PTC patients had the genomic variants of BRAF, especially BRAFV600E mutation, which has been reported to stratify patients and guide clinic-therapies. However, some PTC patients may carry other nonclassical mutation patterns of BRAF, due to the complex of genomic instability. And the spectrum of BRAF mutation was not fully characterized. We reported a novel BRAF mutation pattern of PTC. PATIENT CONCERNS: A 59-year-old woman was admitted to our hospital because of the slight enlargement of bilateral cervical lymph nodes in July 2023. DIAGNOSIS: Ultrasonography revealed that the bilateral thyroid nodules of the patients both presented 1 hypoechoic nodule, which was graded as 3 of the elastic score, and the small calcification in the right lobe (Chinese-Thyroid Imaging Reporting and Data System 4c). Pathological diagnosis showed the interstitial collagen change and focal follicular epithelial papillary hyperplasia with atypical hyperplasia of the bilateral thyroid. Further puncture pathology showed that the patient had a malignant thyroid lesion with the phenotypes of papillary carcinoma and diagnosed with malignancy subsequently. Additionally, the patient harbored a novel insert on BRAF exon 15, a 6-base fragment AGACAG inserting between c.1798 and c.1799. INTERVENTIONS: The patient was undergone on microwave ablation of thyroid carcinoma on July 28, 2023. After the surgery, the patient was treated on anti-infection, cold saline external application of bilateral thyroid swelling supportive treatment. OUTCOMES: No postoperative complications or recurrence and metastasis were found. LESSONS: This is the first case of the novel nonclassical genomic variant of BRAF. Our study extends the spectrum of BRAF mutations. The patient had a favorable response to microwave ablation, indicating that in spite of the association between this mutation and high-grade malignant phenotype, this genomic variant of BRAF did not have a detrimental effect on the response of clinical treatment.

Electric field applications enhance the electron transfer capacity of dissolved organic matter in sludge compost.

Dissolved organic matter (DOM) plays an important role in heavy metal passivation and organic pollutant degradation owing to its redox ability. The structure and composition of DOM are determinants of redox ability changes during composting. Electric field-assisted aerobic composting (EAAC) has been shown to promote the degradation and humification of organic matter in compost. However, how EAAC affects the redox ability of DOM remains unclear. Hence, electron transfer capacity (ETC) of DOM extracted from EAAC was studied using the electrochemical method. Various spectral methods, such as excitation-emission matrix and ultraviolet and visible spectrophotometry were used to study the relationship of ETC with the compositional and structural changes of DOM. Results indicated that EAAC enhanced ETC of DOM at the later stage of composting, and ETC of DOM extracted from the final EAAC product was 10.4% higher than that of the control group. Spectral and correlation analyses showed that EAAC resulted in structural and compositional changes of DOM, and humification degree, aromatic compounds, molecular weight, and fulvic- and humic-like substance contents were improved in EAAC. This conversion increased ETC of DOM. Results of this study will contribute to the understanding of the redox of DOM and in expanding the application of EAAC products.

Effect of ventilation quantity on electron transfer capacity and spectral characteristics of humic substances during sludge composting.

Humic substances (HSs) can ameliorate soil pollution by mediating electron transfer between microorganisms and contaminants. This capability depends on the redox-active functional structure and electron transfer capacity (ETC) of HS. This study mainly aimed to analyze the effects of different ventilation quantities on the ETC and spectral characteristics of HS (including humic acids (HAs) and fulvic acids (FAs)) during sludge composting. HS was extracted from compost with different ventilation quantities (0.1, 0.2, and 0.3 L kg-1 dry matter min-1, denoted as VQ1, VQ2, and VQ3, respectively). The ETC of HS was measured by electrochemical method. Excitation-emission matrix (EEM) spectroscopy, ultraviolet and visible (UV-Vis) spectrophotometry, and Fourier transform infrared (FT-IR) spectroscopy were conducted to understand the evolution of HS composition during composting. Results indicated that the ETC of HA and FA increased during composting, and VQ2 had stronger ETC and electron recycling rate than VQ1 and VQ3 at the end of composting. UV-Vis analysis revealed that the humification degree, aromatization degree, and molecular weight of HA and FA increased during composting, while the content of lignin decreased. EEM-PARAFAC results suggested that VQ2 accelerated the degradation of protein-like substances. FT-IR revealed a decrease trend in polysaccharide and aliphatic, and the carboxyl content increased in VQ2 and VQ3 while decreased in VQ1. Correlation analysis was used to study the relationship between HS components and ETC. The results advance our further understanding of the pollution remediation mechanism of HS.

Enhanced removal of antibiotic resistance genes and mobile genetic elements during sewage sludge composting covered with a semi-permeable membrane.

Transmission of antibiotic resistance genes (ARGs) via air media, such as particulate matter, has been intensively investigated due to human exposure through inhalation. However, whether particulate matter originating from the atmospheric environment of composting plants can impact ARG abundance during composting is unknown. Here, we investigated the effects of the atmospheric environment of composting plants on ARG abundance during sewage sludge composting using semi-permeable membrane-covered thermophilic composting (smTC) and conventional thermophilic composting (cTC). After smTC treatment, the total abundances of ARGs and mobile genetic elements (MGEs) decreased by 42.1 % and 38.1 % compared with those of the initial phase, respectively, but they increased by 4.5- and 1.6-fold after cTC, respectively. This result suggested that smTC was more efficient at decreasing ARGs and MGEs than cTC, mainly due to a significant reduction in bacterial contamination from the atmospheric environment of composting plants that accelerated the resurgence of ARGs and MGEs. Furthermore, culture experiments demonstrated that the abundance and diversity of antibiotic-resistant bacteria during the mature phase of smTC were also significantly (P <  0.05) lower than those in the cTC treatment. Thus, covering composting with a semi-permeable membrane could decrease the risk of ARGs spreading.