Leaf morphometric analysis and potential distribution modelling contribute to taxonomic differentiation in the Quercus microphylla complex.

Mexico is a major center of evolutionary radiation for the genus Quercus, with oak species occurring across different habitat types and showing a wide variation in morphology and growth form. Despite representing about 20% of Mexican species, scrub oaks have received little attention and even basic aspects of their taxonomy and geographic distribution remain unresolved. In this study, we analyzed the morphological and climatic niche differentiation of scrub oak populations forming a complex constituted by six named species, Quercus cordifolia, Quercus frutex, Quercus intricata, Quercus microphylla, Quercus repanda, Quercus striatula and a distinct morphotype of Q. striatula identified during field and herbarium work (hereafter named Q. striatula II). Samples were obtained from 35 sites covering the geographic distribution of the complex in northern and central Mexico. Morphological differentiation was analyzed through geometric morphometrics of leaf shape and quantification of trichome traits. Our results indicated the presence of two main morphological groups with geographic concordance. The first was formed by Q. frutex, Q. microphylla, Q. repanda and Q. striatula, distributed in the Trans-Mexican Volcanic Belt, the Sierra Madre Occidental and a little portion of the south of the Mexican Altiplano (MA). The second group consists of Q. cordifola, Q. intricata and Q. striatula II, found in the Sierra Madre Oriental and the MA. Therefore, our evidence supports the distinctness of the Q. striatula II morphotype, indicating the need for a taxonomic revision. Within the two groups, morphological differentiation among taxa varied from very clear to low or inexistent (i.e. Q. microphylla-Q. striatula and Q. cordifolia-Q. striatula II) but niche comparisons revealed significant niche differentiation in all pairwise comparisons, highlighting the relevance of integrative approaches for the taxonomic resolution of complicated groups such as the one studied here.

Nuclear and chloroplast DNA phylogeography reveals high genetic diversity and postglacial range expansion in Quercus mexicana.

PREMISE: Phylogeographical studies are fundamental for understanding factors that influence the spatial distribution of genetic lineages within species. Population expansions and contractions, distribution shifts, and climate changes are among the most important factors shaping the genetic compositions of populations. METHODS: We investigated the phylogeography of an endemic oak, Quercus mexicana (Fagaceae), which has a restricted distribution in northeastern Mexico along the Sierra Madre Oriental and adjacent areas. Nuclear and chloroplast DNA microsatellite markers were used to describe the genetic diversity and structure of 39 populations of Q. mexicana along its entire distribution area. We tested whether population expansion or contraction events influenced the genetic diversity and structure of the species. We also modeled the historical distributional range of Q. mexicana (for the Mid Holocene, the Last Glacial Maximum, and the Last Interglacial) to estimate the extent to which climate fluctuations have impacted the distribution of this oak species. RESULTS: Our results revealed high genetic diversity and low genetic structure in Q. mexicana populations. Ecological niche models suggested historical fluctuations in the distributional range of Q. mexicana. Historical range changes, gene flow, and physical barriers seem to have played an important role in shaping the phylogeographic structure of Q. mexicana. CONCLUSIONS: Our study indicates that the genetic structure of Q. mexicana may have been the result of responses of oak trees not only to heterogeneous environments present in the Sierra Madre Oriental and adjacent areas, but also to elevational and latitudinal shifts in response to climate changes in the past.

Síntomas depresivos, ansiosos e insomnio, y posibles factores asociados en personal de salud, en un Hospital General en Suramérica, durante la pandemia por COVID-19 en 2020 / Depressive and anxious symptoms and insomnia, and possible associated factors in health workers, in a General Hospital in South America, during the COVID-19 pandemic in 2020

La pandemia por el nuevo coronavirus COVID- 19 ha tenido impacto en la salud mental del personal médico y de enfermería en todo el mundo. Objetivo: identificar la frecuencia de síntomas depresivos, ansiosos e insomnio y los factores posiblemente relacionados con estos desenlaces en el personal sanitario de un hospital de Suramérica durante el primer pico de la pandemia. Materiales y métodos: se aplicaron las escalas PHQ-9 para depresión, GAD- 7 para ansiedad, ISI - 7 para insomnio en 876 trabajadores de la salud del Hospital Pablo Tobón Uribe en la ciudad de Medellín. Resultados: de los 876 participantes (29,2% médicos, 21.2% profesionales de enfermería y 49,5% auxiliares de enfermería), 357 (40.8%) presentaron síntomas depresivos, 300 (34.2%) síntomas ansiosos y 317 (36.2%) insomnio. Se observaron síntomas de depresión, ansiedad e insomnio, con mayor frecuencia en quienes no tenían las necesidades básicas satisfechas y en quienes se sentían estigmatizados por ser personal de salud. Además, la depresión se presentó con más frecuencia en mujeres, la ansiedad en menores de 44 años y el insomnio en personas separadas. Conclusiones: la frecuencia de problemas de salud mental en el personal de salud es considerable. Estos hallazgos demuestran la necesidad de atención en la salud mental de los profesionales médicos y de enfermería durante la pandemia por COVID-19 y la búsqueda de estrategias para mitigar el riesgo en esta población.

Background: pandemic due to novel coronavirus COVID-19 has impacted on the mental health of health care workers all around the world. Material and Methods: this is a cross sectional study in which questionnaires PHQ-9 for depression, GAD-7 for anxiety, ISI-7 for insomnia were virtually and self administered by 876 health care workers laboring in hospital Pablo Tobón Uribe in Medellin city. Results: from 876 participants (29.2% physicians, 21.2% nurses and 49.5% technical nurses), 357 (40.8%) developed depressive symptoms, 300 (34.2%) anxious symptoms and 317 (36.2%) insomnia. Symptoms of depression, anxiety and insomnia were more frequently found in those who did not have basic needs satisfied and in those who felt stigmatized due to being health personal. Besides, depressive symptoms were more frequent in women, anxious symptoms in people younger than 44 years old and insomnia in divorced people. Conclusions: the frequency of mental health problems in health care workers is significant, these findings bring to light the needs for mental health attention in nurses and doctors during COVID-19 pandemic and the research of strategies to mitigate the risk on this population. Feeling stigmatized and not having basic needs satisfied were associated with symptoms of anxiety, depression and insomnia.

First insight into plastics ingestion by fish in the Gulf of California, Mexico.

Plastic particle occurrence in the digestive tracts of fishes from a tropical estuarine system in the Gulf of California was investigated. A total of 1095 fish were analysed, representing 15 species. In total 1384 particles of plastic debris were recovered from the gastrointestinal tracts of 552 specimens belonging to 13 species, and all consisted of threads, the majority of which were small microplastics (0.23 to 1.89), followed by large microplastics (2.07 to 4.49), and few mesoplastics (5.4 to 19.86). Plastic particles were identified using ATR-FTIR spectroscopy. The mean frequency of occurrence of plastics in the gastrointestinal tracts of fishes from this system was 50.5%, which is higher than frequencies reported in similar systems in other areas. The polymers identified by ATR-FTIR were polyamide (51.2%), polyethylene (36.6%), polypropylene (7.3%), and polyacrylic (4.9%). These results show the first evidence of plastic contamination for estuarine biota in the Gulf of California.

A systematic review and trial sequential analysis of intravenous vs. oral peri-operative paracetamol.

Postoperative pain might be different after intravenous vs. oral paracetamol. We systematically reviewed randomised controlled trials in patients >15 years that compared intravenous with oral paracetamol for postoperative pain. We identified 14 trials with 1695 participants. There was inconclusive evidence for an effect of route of paracetamol administration on postoperative pain at 0-2 h (734 participants), 2-6 h (766 participants), 6-24 h (1115 participants) and >24 h (248 participants), with differences in standardised mean (95%CI) pain scores for intravenous vs. oral of -0.17 (-0.45 to 0.10), -0.09 (-0.24 to 0.06), 0.06 (-0.12 to 0.23) and 0.03 (-0.22 to 0.28), respectively. Trial sequential analyses suggested that a total of 3948 participants would be needed to demonstrate a meaningful difference in pain or its absence at 0-2 h. There were no differences in secondary outcomes. Intravenous paracetamol is more expensive than oral paracetamol. Substitution of oral paracetamol in half the patients given intravenous paracetamol in our hospital would save around £ 38,711 (€ 43,960 or US$ 47,498) per annum.

Análisis Biomecánico de una Prótesis de Cadera mediante Elementos Finitos / Biomechanical Analysis of a Hip Prosthesis Using a Finite Element

Resumen En el presente trabajo se plantea un análisis biomecánico de una prótesis de cadera bajo condiciones de cargas estáticas asociadas a actividades cotidianas, en el cual se comparan tres materiales metálicos para la fabricación de una prótesis personalizada a partir de imágenes médicas. Se utilizaron plataformas en la nube de diseño asistido por computadora y de análisis por elementos finitos. Se diseñaron dos modelos de la prótesis a analizar, uno hueco y otro sólido mediante curvas spline paramétricas. Para el análisis biomecánico se requirió un tamaño de malla de 2,537,684 de elementos tetraédricos y 471,335 nodos para estudiar siete casos de posturas para una persona de 75 kg de peso, mismos que se analizaron tomando como materiales base acero inoxidable 316L, aleación Ti-6AL-4V y L-605. Se observó que con actividades tales como trotar, subir y bajar escaleras los materiales 316L y L-605, presentan el riesgo de deformación plástica e inclusive fractura. Los resultados mostraron que el material más idóneo para la fabricación de este tipo de prótesis es el Ti-6Al-4V, además de que este nos permite realizar modelos tanto sólidos como huecos, suponiendo este último, un ahorro de material y proporcionando mayor ligereza en la prótesis.

Abstract This paper shows a biomechanical analysis of a hip prosthesis under conditions of static loads associated with daily activities. For which it compared three metallic materials for the manufacture of a customized prosthesis from medical images, it was used cloud platforms with computer-aided design and finite element analysis. Two models of prosthesis one hollow and the other one solid using parametric spline curves were designed and analyzed. The biomechanical analysis required a mesh size consisting of 2,537,684 tetrahedral elements and 471,335 nodes to study seven cases of postures for a person weighing 75 kg. These cases were analyzed based on 316L stainless steel, Ti-6AL-4V alloy, and another L-605 alloy. It was observed that with activities such as jogging, climbing and descending stairs, materials 316L, and L-605 present the risk of plastic deformation and even fracture. The results show that the most suitable material for the manufacture of this type of prosthesis is the Ti-6Al-4V, which allows us to make both solid and hollow models. Assuming this last material is saved and improves the prosthesis lightness.

Low intensity sonosynthesis of iron carbide@iron oxide core-shell nanoparticles.

Here we demonstrate a simple method for the organic sonosynthesis of stable Iron Carbide@Iron Oxide core-shell nanoparticles (ICIONPs) stabilized by oleic acid surface modification. This robust synthesis route is based on the sonochemistry reaction of organometallic precursor like Fe(CO)5 in octanol using low intensity ultrasonic bath. As obtained, nanoparticles diameter sizes were measured around 6.38 nm ±â€¯1.34 with a hydrodynamic diameter around 25 nm and an estimated polydispersity of 0.27. Core-Shell structure of nanoparticles was confirmed using HR-TEM and XPS characterization tools in which a core made up of iron carbide (Fe3C) and a shell of magnetite (γ-Fe2O3) was found. The overall nanoparticle presented ferromagnetic behavior at 4 K by SQUID. With these characteristics, the ICIONPs can be potentially used in various applications such as theranostic agent due to their properties obtained from the iron oxides and iron carbide phases.

Allosteric modulation model of the mu opioid receptor by herkinorin, a potent not alkaloidal agonist.

Modulation of opioid receptors is the primary choice for pain management and structural information studies have gained new horizons with the recently available X-ray crystal structures. Herkinorin is one of the most remarkable salvinorin A derivative with high affinity for the mu opioid receptor, moderate selectivity and lack of nitrogen atoms on its structure. Surprisingly, binding models for herkinorin are lacking. In this work, we explore binding models of herkinorin using automated docking, molecular dynamics simulations, free energy calculations and available experimental information. Our herkinorin D-ICM-1 binding model predicted a binding free energy of -11.52 ± 1.14 kcal mol-1 by alchemical free energy estimations, which is close to the experimental values -10.91 ± 0.2 and -10.80 ± 0.05 kcal mol-1 and is in agreement with experimental structural information. Specifically, D-ICM-1 molecular dynamics simulations showed a water-mediated interaction between D-ICM-1 and the amino acid H2976.52, this interaction coincides with the co-crystallized ligands. Another relevant interaction, with N1272.63, allowed to rationalize herkinorin's selectivity to mu over delta opioid receptors. Our suggested binding model for herkinorin is in agreement with this and additional experimental data. The most remarkable observation derived from our D-ICM-1 model is that herkinorin reaches an allosteric sodium ion binding site near N1503.35. Key interactions in that region appear relevant for the lack of ß-arrestin recruitment by herkinorin. This interaction is key for downstream signaling pathways involved in the development of side effects, such as tolerance. Future SAR studies and medicinal chemistry efforts will benefit from the structural information presented in this work.

Coexistencia de vitiligo y psoriasis, serie de casos y perspectivas etiopatogénicas / Coexistence of vitiligo and psoriasis, case series and etiopathogenic perspectives

El vitiligo y la psoriasis se observan con frecuencia en la consulta dermatológica; sin embargo, pese a su alta prevalencia, no es frecuente que ambas se encuentren en un mismo paciente. Presentamos los casos de tres pacientes que exhiben esta situación y realizamos una revisión de la literatura, en cuanto a los aspectos fisiopatogénicos comunes, que podrían contribuir a generar la coexistencia de estas entidades.

Vitiligo and psoriasis are commonly seen in the dermatology clinic; yet, despite their high prevalence, it is infrequent to find them together in the same patient. We report three cases exhibiting this situation and we do a literature review regarding common pathophysiologic aspects that could generate the coexistence of these entities.

Coexistencia de vitiligo y psoriasis, serie de casos y perspectivas etiopatogénicas / Coexistence of vitiligo and psoriasis, case series and etiopathogenic perspectives

El vitiligo y la psoriasis se observan con frecuencia en la consulta dermatológica; sin embargo, pese a su alta prevalencia, no es frecuente que ambas se encuentren en un mismo paciente. Presentamos los casos de tres pacientes que exhiben esta situación y realizamos una revisión de la literatura, en cuanto a los aspectos fisiopatogénicos comunes, que podrían contribuir a generar la coexistencia de estas entidades.(AU)

Vitiligo and psoriasis are commonly seen in the dermatology clinic; yet, despite their high prevalence, it is infrequent to find them together in the same patient. We report three cases exhibiting this situation and we do a literature review regarding common pathophysiologic aspects that could generate the coexistence of these entities.(AU)

Eficacia de la Lamotrigina en pacientes epilépticos fármaco resistentes a tratamientos en un centro neurológico de referencia en la ciudad de Cali / Efficacy of Lamotrigine in epilepsy patients resistant to drug tratments in a neurological reference center in the city of Cali

Introducción: la epilepsia es una enfermedad neurológica frecuente, con una incidencia estimada de 50 por cada 100,000 habitantes y una prevalencia de cinco a diez por 1,000 habitantes en los países desarrollados. Se le llama refractaria cuando las crisis epilépticas son tan frecuentes que limitan la habilidad del paciente para vivir plenamente o cuando los efectos secundarios del manejo tratamiento son limitantes para el desarrollo normal de la persona pese al mejor manejo médico instaurado. La Lamotrigina es un antiepiléptico que inhibe los canales de sodio voltaje-dependientes, modulando la liberación presináptica de transmisores excitatorios de tipo glutamato y aspartato. Objetivo: determinar la efectividad de la Lamotrigina como monoterapia o en asociación con fármacos antiepilépticos convencionales en pacientes con Epilepsia Refractaria. Materiales y métodos: estudio descriptivo. Periodo de tiempo: mayo 2009 a noviembre 2012. Análisis de las variables por medio de frecuencias, promedios y proporciones. Resultados: muestra: 115 historias clínicas de pacientes con epilepsia refractaria. El 97,4% (112) tiene reducción en el número de crisis, siendo en el 85,2% (98) mayor o igual al 50%. Hubo un promedio de 9,45 crisis por mes, con una mediana de seis episodios, previo al manejo con este medicamento, mientras que tras la administración del medicamento, el promedio de episodios fue de 3,65 por mes, con mediana de 1 episodio/mes. El promedio de fármacos utilizados antes de introducir la Lamotrigina fue de 1,9 con un uso mínimo de 1 y máximo 6 y a partir del inicio de la Lamotrigina el promedio fue 2,3, con uso mínimo de 1 y máximo de 4 medicamentos. Conclusión: la Lamotrigina es un medicamento de última generación con adecuada efectividad y pocos efectos adversos que puede reducir de forma efectiva la frecuencia de crisis epilépticas en los pacientes de difícil manejo con refractariedad farmacológica.

Introduction: Epilepsy is a common neurological condition with an estimated incidence of 50 per 100,000 population and a prevalence five to ten per 1,000 population in developed countries. It is considered refractory when seizures are so frequent that the patient's ability to live fully is limited, when treatment does not control seizures, or when side effects are limiting for the normal development of the person. Lamotrigine is an antiepileptic drug that inhibits the sodium channel voltage-dependent presynaptic modulating excitatory transmitter glutamate and aspartate type. Objective: To determine the effectiveness of Lamotrigine as monotherapy or in combination with conventional antiepileptic drugs in patients with refractory epilepsy. Materials and methods: A descriptive study. Period May 2009 to November 2012. Variable analysis by frequency, averages and ratios. Results: Sample: 115 medical records. 97.4% (112) of the patients have reduced the number of seizures, so than 85.2% (98) have had a reduction greater than or equal to 50%. There was an average of 9.45 attacks per month, with a median of 6 episodes, prior to treatment with this medicine, whereas after administering medication, the average was 3.65 episodes per month, with a median of 1 crisis/month. The average drugs used before initiating the Lamotrigine was 1.9 with a minimum of 1 and maximum of 6 and from the beginning of the Lamotrigine the average was 1.54, with minimum use of 1 and maximum of 4 drugs. Conclusion: Lamotrigine is a last generation drug with great effectiveness and few side effects, which can effectively reduce the frequency of seizures in patients with refractory epilepsy.

Polymorphisms in the GSTT1 and GSTM1 genes are associated with increased risk of preeclampsia in the Mexican mestizo population.

Preeclampsia is a pregnancy-specific disorder in humans and a major cause of maternal and neonatal morbidity and mortality. Increasing evidence suggests that oxidative stress plays an important role in the pathogenesis of preeclampsia. The aim of this study was to investigate the relationship between null alleles of the glutathione S-transferases (GST) M1 and T1 genes and the risk of preeclampsia. This case-control study involved 112 preeclamptic and 233 normoevolutive pregnant women. The null polymorphisms were genotyped by multiplex polymerase chain reaction (PCR). Our results showed an increased risk of preeclampsia in patients with the GSTT1 null genotype [odds ratio (OR) = 2.21; 95% confidence interval (CI) = 1.14-4.27; P = 0.018]. Our data further showed that a combination of deletion genotypes of the GSTM1 and GSTT1 genes conferred an even higher risk of preeclampsia (OR = 4.56, 95%CI = 1.59-13.09; P = 0.005). Our results provide the first evidence suggesting that a GSTT1 null polymorphism might be associated with preeclampsia in the Mexican mestizo population, and that this risk increases with the combination of both GSTT1 and GSTM1 null polymorphisms.

First Report of Monilinia fructicola Causing Brown Rot on Stored Japanese Plum Fruit in Chile.

In autumn 2013, fruit of Japanese plum (Prunus salicina) cvs. Angelino and Black Kat developed an unusual brown and soft rot after 2 months in cold storage (0°C) on nearly 1% of the fruit. Fruit showed small, circular, light brown spots that eventually destroyed the entire fruit. Small sporodochia appeared on the fruit surface. Fruit was harvested from orchards located near San Francisco de Mostazal (33°59' S, 70°41' W), Chile. Small pieces of diseased tissue were selected from margins of lesions of surface disinfected (96% ethanol) fruit (n = 7) and placed on acidified potato dextrose agar (PDA) plates for 5 days at 20°C. Light brown colonies with even margins and concentric rings of spores were obtained. The conidia of five isolates were one-celled, hyaline, lemon-shaped, (min. 10.7) 14.9 ± 1.5 (max. 18.6) × (min. 8.1) 9.4 ± 0.8 (max. 10.8) µm (n = 30), and borne in branched monilioid chains. This fungus was identified as Monilinia fructicola (G. Winter) Honey (1). Identification was confirmed by amplifying and sequencing the ribosomal ITS1-5.8S-ITS2 region using ITS1 and ITS4 primers (3). BLAST analysis of Chilean plum isolates (GenBank Accession Nos. KF148610 and KF148611) were 99 to 100% identical to isolates of M. fructicola originating from the United States (DQ314727 and HQ846966, respectively) and 100% identical to the first Chilean isolate (JN001480) found in nectarines originating from California at the supermarkets in Santiago in June 2009. Koch's postulates were fulfilled by reproducing brown rot symptoms on mature wounded Japanese plums cv. Angelino (n = 8) inoculated with 10 µl of a conidial suspension (105 conidia/ml) or with a mycelium plug (5-mm diameter). After 2 days in humid chambers (>80% relative humidity) at 25°C, all inoculated fruit developed brown rot symptoms with necrotic lesion means of 15.8 and 21.5 mm in diameter in fruit inoculated with conidia and mycelium, respectively. Non-inoculated control fruit remained healthy. Re-isolations were performed on PDA and the presence of M. fructicola was morphologically confirmed in 100% of the symptomatic fruits. To our knowledge, this is the first report demonstrating the presence of M. fructicola causing brown rot in stored Japanese plums in Chile after its first interception in 2009 in Chile, suggesting that this pathogen has been established in the field. Currently, M. fructicola is a quarantine organism under official control, restricted to Prunus orchards between Santiago and Nancagua in central Chile (2). References: (1) EPPO. EPPO Bull. 39:337, 2009. (2) Servicio Agrícola y Ganadero, SAG, Ministerio de Agricultura, Gobierno de Chile. www.sag.cl , accessed 15 November 2013. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, NY, 1990.

Millimeter-scale resolution of trace metal distributions in microbial mats from a hypersaline environment in Baja California, Mexico.

Microbial mats from two ponds with different salinities from the saltern of Guerrero Negro (Mexico) points toward millimeter-scale coherent variations in trace metal (Me) concentrations (Cd, Co, Cu, Fe, Mn, Ni, Pb, Zn). Total, HCl-leachable and pyrite-associated Me showed a trend of increasing concentrations with increasing depth suggesting gradual addition of reactive Me probably as a result of metal sulfide precipitation at depth. The trends in Me profiles can be ascribed to the establishment and maintenance of microzones that promote geochemical processes, bacterial population distributions, and differential mass transport within the mats. Degrees of trace metal pyritization (1 ± 1% for Zn to 24 ± 7% for Cd) as well as metals associated with the pyrite fraction (<1.4-36 ± 18 nmol g(-1) for Zn and Mn, respectively) were low, as expected from a reactive Fe-limited system like Guerrero Negro. Calculated enrichment factors showed that Ni (2.6 ± 2.1), Co (5.5 ± 4.0), Pb (9.4 ± 7.4), and Cd (57 ± 39) were, on average, enriched in the microbial mats of Guerrero Negro. Natural enrichments of Cd, Pb, and Co in sediments along the coast of Baja California and metabolical requirements of Co and Ni by the predominant cyanobacteria in the Guerrero Negro mats may explain these enrichments. Metal characteristics in microbial mats could be advantageously used as biosignatures to identify their presence in the geological record or in other planetary systems.

[Evolution of the intake and nutritional status of zinc, iron and copper in women undergoing bariatric surgery until the second year after surgery]. / Evolución de la ingesta y del estado nutricional de zinc, hierro y cobre en mujeres sometidas a cirugía bariátrica hasta el segundo año postoperatorio.

INTRODUCTION: Bariatric surgery allows a significant reduction in weight and improvement of comorbidities associated with obesity in the long term, but it can also adversely affect the nutritional status of some micronutrients. OBJECTIVES: To evaluate changes in intake and parameters of nutritional status of zinc, iron and copper in patients undergoing Roux-en-Y gastric bypass (GBP) or sleeve gastrectomy (SG), until the second postoperative year. METHODS: We prospectively studied 45 women undergoing GBP or SG (mean age 35.2 ± 8.4 years, mean BMI 39.8 ± 4.0 kg/m²), every 6 months We measured intake and status indications nutritional zinc, iron and copper, and annually evaluated body composition. The contribution of minerals through supplements represented twice the recommended intake for a healthy woman in patients undergoing GT and three times for GBP. RESULTS: 20 women underwent GBP and 25 SG. In both groups there was a significant reduction in weight and body fat percentage, which was maintained until the second postoperative year. Women who have had a greater commitment GBP nutritional status of zinc, iron and copper, that patients undergoing SG. CONCLUSIONS: Gastric bypass Roux-Y produces a greater commitment of nutritional status of zinc, iron and copper sleeve gastrectomy. It should evaluate whether administration of supplementation fractional improve the absorption of these nutrients.

Aportes de los nuevos enfoques para la conformación de la salud pública alternativa / Contributions of new approaches to the formation of alternative public health

This article discusses the possibility of a different way from traditional to raise health problems in public health context, answering its guiding question: How the contemporary approaches to public heath help to understand it and size its projection in the coming years? Initially, we will make a brief characterization of conventional public health, in order to show its limitations to answer people’s health problems. Then we enter to the contemporary approaches or tendencies, specifically those developed in the critical school framework, because it’s on them where the approaches that significantly contribute to the health public conformation converge. Among the selected currents we have: the critical theory, the social medicine, the collective health, social determinants of health, the gender approach and the complexity theory, approaches that contribute to a different way of public health working and to have always in mind the importance of the subject in the boarding of health problems. Likewise, the technologic reason directed knowledge must be changed in order to reach the multiculturalism, this accepting other knows as valid to the construction of know.

Tromboembolismo venoso postoperatorio: grave riesgo prevenible / Postoperatory venous thromboembolism: a serious preventable risk

La trombosis venosa profunda y el tromboembolismo pulmonar postoperatorio son complicaciones que tienen influencia en la morbimortalidad. Múltiples investigaciones evidencian sus cifras y la reducción del riesgo con la tromboprofilaxis. Han sido evaluados diferentes medicamentos y su nivel de protección, pero el seguimiento de las guías de práctica clínica publicadas no es óptimo debido a la falta de claridad en el manejo de pacientes con factores de riesgo intermedio. La prevención demostrada por la tromboprofilaxis obliga a implementar estrategias para detectar pacientes en riesgo y seguir las guías.

Postoperative deep venous thrombosis and pulmonary thromboembolism are complications which have incidence on morbimortality. Many researches evidence their figures and how thromboprophylaxis can reduce these risks. Different drugs and their level of protection have been evaluated, but the adherence to the published clinical practice guidelines is not optimal because of the lack of clarity in the management of patients with intermediate risk factors. The proved prevention with thromboprophylaxis forces to implement strategies to detect the patients at risk and to follow the guidelines.

[Alopecia in women with severe and morbid obesity who undergo bariatric surgery]. / Alopecia en mujeres con obesidad severa y mórbida sometidas a cirugía bariátrica.

INTRODUCTION: Bariatric surgery leads to a significant body weigh reduction although it is associated to a higher risk of presenting some nutritional deficiencies. A common complication, little studied and mainly related to zinc deficiency is alopecia. OBJECTIVES: To compare the nutritional status of zinc, iron, copper, selenium and protein-visceral in women with different degrees of hair loss at 6 months after gastric bypass or tubular gastrectomy. METHODS: The patients were categorized into two groups according to the degree of hair loss: group 1 or mild loss (n = 42) and group 2 or severe hair loss (n = 45). Zinc, iron, copper, and selenium, as well as the indicators of the nutritional status of zinc, iron, copper, and proteinvisceral were assessed before and after 6 months of the surgery. RESULTS: In both groups there was a significant body weight reduction at 6 months post-surgery (-38.9% ± 16.4%). Patients in group 1 presented a significantly higher intake of zinc (20.6 ± 8.1 vs. 17.1 ± 7.7 mg/d) and iron (39.7 ± 35.9 vs. 23.8 ± 21.3 mg/d.), and lower compromise in the nutritional status of zinc and iron than group 2. However, patients in group 2 had lower compromise in the nutritional status of copper. There were no differences regarding the plasma concentrations of albumin. CONCLUSIONS: The patients having lower hair loss at six months after surgery had higher zinc and iron intake and lower compromise of the nutritional status of both minerals.

First Report of Nothofagus macrocarpa Dieback Caused by Phytophthora citrophthora and P. nicotianae in Chile.

The genus Nothofagus, family Nothofagaceae, comprises 36 species of trees that are native to the Southern Hemisphere. N. macrocarpa (DC.) F.M. Vásquez & R.A. Rodríguez (Roble de Santiago) is an important deciduous tree, endemic to central Chile (32 to 35°S), and found above 800 m altitude. There is an increasing interest in N. macrocarpa as an ornamental. However, a general dieback (40 to 50% prevalence) was observed at a commercial nursery in Santiago in 2009, limiting its multiplication. Symptoms are wilting, partial defoliation, reddish brown cankers on the crowns, and root necrosis. The purpose of this work was to study the etiology of the dieback in nurseries. Phytophthora was isolated from the roots and cankers of symptomatic plants (n = 3) and soil samples (using apples and avocados as baits) on amended corn meal agar (3) at 20°C for 5 days in the dark. Morphologically, P. citrophthora (Smith & Smith) Leonian, and P. nicotianae Breda de Haan were identified (2). On V8 juice agar (V8) (1), P. citrophthora formed petaloid colonies, grew between 5 and 30°C (optimum of 25°C), and produced deciduous, mono- or bipapillated sporangia of (28.1) 45.0 to 64.1 × (18.8) 32.0 to 39.2 µm. On V8, P. nicotianae produced cottony colonies, grew between 10 and 30°C (optimum of 25°C), and produced spherical, intercalary chlamydospores (mean diameter of 19.6 µm) and persistent, papillate, spherical to ovoid, ellipsoid, obpyriform sporangia of (33.2) 47.5 to 67.6 × (24.1) 30.0 to 48.9 µm. Isolates of P. citrophthora were sexually sterile, but P. nicotianae formed oogonia with amphigenous antheridia in dual cultures with P. cinnamomi (A2 compatibility type). BLAST analysis of the internal transcribed spacer (ITS) region of rDNA of isolates identified as P. citrophthora (IMI 399056 and IMI 399054, GenBank Accession Nos. JF699756 and JF699755) and P. nicotianae (IMI 399055, Accession No. JF699757), amplified by PCR using ITS universal primers (4), revealed 100% similarity with reference isolates of P. citrophthora (Accession Nos. GU259324.1 and GU259317.1) and P. nicotianae (Accession No. GU983635.1). P. citrophthora (n = 2) and P. nicotianae (n = 1) were pathogenic when wounded detached twigs (n = 5) of N. macrocarpa and N. obliqua were inoculated with 20 µl of a mycelial suspension (106 CFU/ml) of either Phytophthora spp. Twigs were placed in a moist chamber at 20°C for 12 days prior to determine the length of the necrotic lesions that developed. An equal number of noninoculated twigs were left as control. Reisolation of P. citrophthora and P. nicotianae from inoculated material was 100%. The length of the necrotic lesions (13 to 80 mm) from inoculated N. macrocarpa and N. obliqua was significantly greater (P < 0.05) compared with the controls. Regardless of Phytophthora isolates, necrotic lesions (53.9 ± 15.8 mm) in infected N. macrocarpa were significantly longer than in N. obliqua (28.6 ± 13.1 mm) (P < 0.0001). To our knowledge, this is the first report of P. citrophthora and P. nicotianae associated with dieback on N. macrocarpa in Chile. Therefore, there is a potential risk of Phytophthora dieback in N. macrocarpa in nature. References: (1) J. Ampuero et al. Plant Dis. 92:1529, 2008. (2) D. C. Erwin and O. K. Ribeiro. Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN, 1996. (3) B. A. Latorre and R. Muñoz. Plant Dis. 77:715, 1993. (4) T. J. White et al. PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.