ABSTRACT
The influence of light spectral properties on circadian rhythms is of substantial interest to laboratory-based investigation of the circadian system and to field-based understanding of the effects of artificial light at night. The trade-offs between intensity and spectrum regarding masking behaviors are largely unknown, even for well-studied organisms. We used a custom LED illumination system to document the response of wild-type house mice (Mus musculus) to 1-h nocturnal exposure of all combinations of four intensity levels (0.01, 0.5, 5 and 50â lx) and three correlated color temperatures (CCT; 1750, 1950 and 3000â K). Higher intensities of light (50â lx) suppressed cage activity substantially, and consistently more for the higher CCT light (91% for 3000â K, 53% for 1750â K). At the lowest intensity (0.01â lx), mean activity was increased, with the greatest increases for the lowest CCT (12.3% increase at 1750â K, 3% increase at 3000â K). Multiple linear regression confirmed the influence of both CCT and intensity on changes in activity, with the scaled effect size of intensity 3.6 times greater than that of CCT. Activity suppression was significantly lower for male than for female mice. Assessment of light-evoked cFos expression in the suprachiasmatic nucleus at 50â lx showed no significant difference between high and low CCT exposure. The significant differences by spectral composition illustrate a need to account for light spectrum in circadian studies of behavior, and confirm that spectral controls can mitigate some, but certainly not all, of the effects of light pollution on species in the wild.