Conjoint analysis of physio-biochemical, transcriptomic, and metabolomic reveals the response characteristics of solanum nigrum L. to cadmium stress.

Cadmium (Cd) is a nonessential element in plants and has adverse effects on the growth and development of plants. However, the molecular mechanisms of Cd phytotoxicity, tolerance and accumulation in hyperaccumulators Solanum nigrum L. has not been well understood. Here, physiology, transcriptome, and metabolome analyses were conducted to investigate the influence on the S. nigrum under 0, 25, 50, 75 and 100 µM Cd concentrations for 7 days. Pot experiments demonstrated that compared with the control, Cd treatment significantly inhibited the biomass, promoted the Cd accumulation and translocation, and disturbed the balance of mineral nutrient metabolism in S. nigrum, particularly at 100 µM Cd level. Moreover, the photosynthetic pigments contents were severely decreased, while the content of total protein, proline, malondialdehyde (MDA), H2O2, and antioxidant enzyme activities generally increased first and then slightly declined with increasing Cd concentrations, in both leaves and roots. Furthermore, combined with the previous transcriptomic data, numerous crucial coding-genes related to mineral nutrients and Cd ion transport, and the antioxidant enzymes biosynthesis were identified, and their expression pattern was regulated under different Cd stress. Simultaneously, metabolomic analyses revealed that Cd treatment significantly changed the expression level of many metabolites related to amino acid, lipid, carbohydrate, and nucleotide metabolism. Metabolic pathway analysis also showed that S. nigrum roots activated some differentially expressed metabolites (DEMs) involved in energy metabolism, which may enhance the energy supply for detoxification. Importantly, central common metabolism pathways of DEGs and DEMs, including the "TCA cycle", "glutathione metabolic pathway" and "glyoxylate and dicarboxylate metabolism" were screened using conjoint transcriptomics and metabolomics analysis. Our results provide some novel evidences on the physiological and molecular mechanisms of Cd tolerance in hyperaccumulator S. nigrum plants.

Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019.

Introduction: Paphiopedilum barbigerum is currently the rarest and most endangered species of orchids in China and has significant ornamental value. The mature seeds of P. barbigerum are difficult to germinate owing to the absence of an endosperm and are highly dependent on mycorrhizal fungi for germination and subsequent development. However, little is known about the regulation mechanisms of symbiosis and symbiotic germination of P. barbigerum seeds. Methods: Herein, transcriptomics and proteomics were used to explore the changes in the P. barbigerum seeds after inoculation with (FQXY019 treatment group) or without (control group) Epulorhiza sp. FQXY019 at 90 days after germination. Results: Transcriptome sequencing revealed that a total of 10,961 differentially expressed genes (DEGs; 2,599 upregulated and 8,402 downregulated) were identified in the control and FQXY019 treatment groups. These DEGs were mainly involved in carbohydrate, fatty acid, and amino acid metabolism. Furthermore, the expression levels of candidate DEGs related to nodulin, Ca2+ signaling, and plant lectins were significantly affected in P. barbigerum in the FQXY019 treatment groups. Subsequently, tandem mass tag-based quantitative proteomics was performed to recognize the differentially expressed proteins (DEPs), and a total of 537 DEPs (220 upregulated and 317 downregulated) were identified that were enriched in processes including photosynthesis, photosynthesis-antenna proteins, and fatty acid biosynthesis and metabolism. Discussion: This study provides novel insight on the mechanisms underlying the in vitro seed germination and protocorm development of P. barbigerum by using a compatible fungal symbiont and will benefit the reintroduction and mycorrhizal symbiotic germination of endangered orchids.

Symbiotic seed germination and seedling growth of mycorrhizal fungi in Paphiopedilum hirsutissimun (Lindl.Ex Hook.) Stein from China.

Similar to other orchid species, Paphiopedilum hirsutissimum (Lindl.ex Hook.) Stein, relies on nutrients provided by mycorrhizal fungus for seed germination and seedling development in the wild owing to a lack of endosperm in its seeds. Therefore, obtaining suitable and specialized fungi to enhance seed germination, seedling formation, and further development is considered a powerful tool for orchid seedling propagation, reintroduction, and species conservation. In this study, we investigated the diversity, abundance, and frequency of endophytic fungal strains in the root organs of P. hirsutissimum. One family and five genera of the fungi were isolated and identified through rDNA-ITS sequencing. The ability of isolated fungi to germinate in vitro from the seeds of this species was evaluated, and the development of P. hirsutissimum protocorm has been described. The findings showed that the treatments inoculated with endophytic fungal DYXY033 may successfully support the advanced developmental stage of seedlings up to stage 5. In addition, scanning electron microscopy (SEM) revealed that the mycelium of this strain began to invade from either end of the seeds up to the embryo, extending rapidly from the inside to the outside. Its lengthening resulted in the bursting of the seed coat to form protocorms, which developed into seedlings. The results showed that DYXY033 has a high degree of mycobiont specificity under in vitro symbiotic seed germination conditions and is a representative mycorrhizal fungus with ecological value for the species. In summary, this strain may particularly be significant for the protection of P. hirsutissimum species that are endangered in China. In the long run, it may also contribute to global efforts in reintroducing orchid species and in realizing in situ restorations of threatened orchid populations.

Unraveling the role of multi-walled carbon nanotubes in a corn-soil system: Plant growth, oxidative stress and heavy metal(loid)s behavior.

In the age of nanotechnological advancement, carbon nanotubes (CNTs) are drawing global attention. However, few studies have been published on the crop growth responses to CNTs in heavy metal(loid)s contaminated environments. A pot experiment was conducted to assess the effect of multi-walled carbon nanotubes (MWCNTs) on plant development, oxidative stress, and heavy metal(loid)s behavior in a corn-soil system. Corn (Zea mays L.) seedlings were cultivated in soil containing Cadmium (Cd) and Arsenic (As) that had been primed with 0, 100, 500, and 1000 mg kg-1 MWCNTs. The application of 100 and 500 mg kg-1 MWCNTs improved shoot length by 6.45% and 9.21% after 45 days, respectively. Total plant dry biomass increased by 14.71% when treated with 500 mg kg-1 MWCNTs but decreased by 9.26% when exposed to 1000 mg kg-1 MWCNTs. MWCNTs treatment did not affect Cd accumulation in plants. On the other hand, the bio-concentration factor of As was inversely associated with plant growth (p < 0.05), which was declined in MWCNTs treatments. Oxidative stress was aggravated when plants were exposed to MWCNTs, thus activating the antioxidant enzymes system in the corn. In contrast, TCLP-extractable Cd and As in soil significantly decreased than in the control. Additionally, the soil nutrients were changed under MWCNTs treatments. Our findings also revealed that a particular concentration of MWCNTs can mitigate the toxicity of Cd and As in corn seedlings. Therefore, these results suggest the prospective application of CNTs in agricultural production, ensuring environmental and soil sustainability.

Spatial Heterogeneity of Soil Bacterial Community Structure and Enzyme Activity along an Altitude Gradient in the Fanjingshan Area, Northeastern Guizhou Province, China.

Changes in altitude can cause regional microclimate changes, leading to the spatial heterogeneity of environmental factors and soil bacteria. However, the internal driving process and mechanism remain unclear. Here, we selected Fanjingshan, a typical nature reserve in the subtropical region of south China with a clear altitudinal belt, to reveal the response mechanisms of microbial populations with altitude changes. We examined the physiochemical and biological properties (pH and soil enzyme activities) of 0~10 cm soil layers, soil bacterial diversity, and community structure across the 2.1 km belt (consisting of six altitude ranges). Our results showed that soil pH was highest at the altitude range below 900 m and decreased with altitude thereafter. Soil enzyme activities showed an overall decreasing trend with altitude rising. The soil sucrase and catalase activity was highest (48.35 mg.g-1.d-1 and 23.75 µmol.g-1, respectively) at altitudes below 900 m; the soil urease activity was highest (704.24 µg.g-1.d-1) at 900~1200 m; and the soil acid phosphatase activity was highest (57.18 µmol.g-1) at 1200~1500 m. In addition, the soil bacterial community diversity showed a linear increasing trend, with the maximum abundance at 1500~1800 m. Soil pH was correlated with enzyme activity and bacterial community composition and structure, and the correlation was the strongest between pH and the distribution of bacterial diversity at altitudes below 900 m. Overall, soil enzyme activities and soil bacterial diversity showed spatial heterogeneity along the altitude gradient, and their community structure and composition were affected by altitude as a result of changes in soil physicochemical factors. This study provides a better and deeper understanding of the spatial succession of soil in the Fanjingshan area and the distribution pattern of soil microorganisms in central subtropical mountain ecosystems.

Insights into growth-promoting effect of nanomaterials: Using transcriptomics and metabolomics to reveal the molecular mechanisms of MWCNTs in enhancing hyperaccumulator under heavy metal(loid)s stress.

Carbon nanotubes present potential applications in soil remediation, particularly in phytoremediation. Yet, how multi-walled carbon nanotubes (MWCNTs) induced hyperaccumulator growth at molecular level remains unclear. Here, physio-biochemical, transcriptomic, and metabolomic analyses were performed to determine the effect of MWCNTs on Solanum nigrum L. (S. nigrum) growth under cadmium and arsenic stresses. 500 mg/kg MWCNTs application significantly promoted S. nigrum growth, especially for root tissues. Specially, MWCNTs application yields 1.38-fold, 1.56-fold, and 1.37-fold enhancement in the shoot length, root length, and fresh biomass, respectively. Furthermore, MWCNTs significantly strengthened P and Fe absorption in roots, as well as the activities of antioxidative enzymes. Importantly, the transcriptomic analysis indicated that S. nigrum gene expression was sensitive to MWCNTs, and MWCNTs upregulated advantageous biological processes under heavy metal(loid)s stress. Besides, MWCNTs reprogramed metabolism that related to defense system, leading to accumulation of 4-hydroxyphenylpyruvic acid (amino acid), 4-hydroxycinnamic acid (xenobiotic), and (S)-abscisic acid (lipid). In addition, key common pathways of differentially expressed metabolites and genes, including "tyrosine metabolism" and "isoquinoline alkaloid biosynthesis" were selected via integrating transcriptome and metabolome analyses. Combined omics technologies, our findings provide molecular mechanisms of MWCNTs in promoting S. nigrum growth, and highlight potential application of MWCNTs in soil remediation.

Two plant growth-promoting bacterial Bacillus strains possess different mechanisms in affecting cadmium uptake and detoxification of Solanum nigrum L.

Microorganisms affect cadmium (Cd) extraction by hyperaccumulators to varying degrees, but the potential mechanism has not been completely studied. Here, two plant growth-promoting bacteria (PGPB, Bacillus paranthracis NT1 and Bacillus megaterium NCT-2) were assessed for their influence on Cd uptake by Solanum nigrum L. and their influence mechanisms. The results showed that both two strains could regulate phytohormones secretion, alleviate oxidative stress and promote S. nigrum growth when exposed to Cd (dry weight was significantly increased by 21.51% (strain NCT-2) and 21.23% (strain NT1) compared with the control, respectively). Additionally, strain NCT-2 significantly elevated the translocation factor (TF) and bioconcentration factor (BCF), and thus significantly facilitated total Cd uptake by 41.80% of S. nigrum, whereas strain NT1 significantly reduced the BCF and TF, resulting in insignificant effect on total Cd uptake of S. nigrum compared with the control. Results of qPCR illustrated that the two strains influenced the detoxification of Cd in S. nigrum by affecting the expression of antioxidant enzyme genes and gene PDR2. Moreover, the differential expression of heavy metal transport genes IRT1 and HMA may lead to the difference of Cd accumulation in S. nigrum. Principal component analysis and Pearson correlation coefficient analysis further verified the positive roles of salicylic acid and indole-3-acetic acid on Cd detoxification of S. nigrum, and the positive correlation relationship between transportation of Cd from underground to shoot, plant biomass and Cd uptake. Altogether, our results demonstrated that these two PGPB have great potential in helping plants detoxify Cd and could provide insights into the mechanism of PGPB-assisted phytoremediation of Cd-contaminated soil.

A sustainable approach for bioremediation of secondary salinized soils: Studying remediation efficiency and soil nitrate transformation by bioaugmentation.

Nitrate is the main nitrogen source for plant growth, but it can also pollute the environment. A major cause of soil secondary salinization is the rising level of nitrates in the soil, which poses a threat to the sustainability and fertility of global greenhouse soils. Herein, Bacillus megaterium NCT-2 was used as a microbial agent to remove nitrate by bioaugmentation, and the remediation efficiency of secondary salinized soil in different degrees was evaluated. The findings showed that the highest nitrate removal rate of 62.76% was in a medium degree of secondary salinized soil. Moreover, the results of 16S rRNA high-throughput sequencing and quantitative real-time PCR (qPCR) demonstrated that NCT-2 agent reduced the microbial diversity, increased the microbial community stability, and changed the composition and function of the microbial community were changed by NCT-2 agent in all districts soil. Further analysis demonstrated that the NCT-2 bacterial agent significantly increased the key enzyme genes of the assimilation pathway (nitrite reductase gene NasD, 87-404 times, and glutamine reduction enzyme gene GlnA, 13-52 times) and dissimilatory reduction to ammonium (DNRA) (nitrate reductase gene NarG, 14-56 times) in different degrees of secondary salinized soils. This proved that NCT-2 agent could promote the nitrate assimilation and the dissimilation and reduction to ammonium in secondary salinized soil. Thus, the current findings suggested that the NCT-2 agent has a significant potential for reducing excessive nitrate levels in secondary salinized soil. The remediation efficiency was related to the microbial community composition and the degree of secondary salinization. This study could provide a theoretical basis for the remediation of secondary salinized soil in the future.

Comparative cytology combined with transcriptomic and metabolomic analyses of Solanum nigrum L. in response to Cd toxicity.

Cadmium (Cd) triggers molecular alterations in plants, perturbs metabolites and damages plant growth. Therefore, understanding the molecular mechanism underlying the Cd tolerance in plants is necessary for assessing the persistent environmental impact of Cd. In this study, Solanum nigrum was selected as the test plant to investigate changes in biomass, Cd translocation, cell ultrastructure, metabolites and genes under hydroponic conditions. The results showed that the plant biomass was significantly decreased under Cd stress, and the plant has a stronger Cd transport capability. Transmission electron microscopy revealed that increased Cd concentration gradually damaged the plant organs (roots, stems and leaves) cell ultrastructure, as evidenced by swollen chloroplasts and deformed cell walls. Additionally, metabolomics analyses revealed that Cd stress mainly affected seven metabolism pathways, including 19 differentially expressed metabolites (DEMs). Moreover, 3908 common differentially expressed genes (DEGs, 1049 upregulated and 2859 downregulated) were identified via RNA-seq among five Cd treatments. Meanwhile, conjoint analysis found several DEGs and DEMs, including laccase, peroxidase, D-fructose, and cellobiose etc., are associated with cell wall biosynthesis, implying the cell wall biosynthesis pathway plays a critical role in Cd detoxification. Our comprehensive investigation using multiple approaches provides a molecular-scale perspective on plant response to Cd stress.

When nanoparticle and microbes meet: The effect of multi-walled carbon nanotubes on microbial community and nutrient cycling in hyperaccumulator system.

Carbon nanotubes can potentially stimulate phytoremediation of heavy metal contaminated soil by promoting plant biomass and root growth. Yet, the regulating mechanism of carbon nanotubes on the rhizosphere microenvironment and their potential ecological risks remain poorly characterized. The purpose of this study was to systematically evaluate the effects of multi-walled carbon nanotubes (MCNT) on the diversity and structure of rhizosphere soil bacterial and fungal communities, as well as soil enzyme activities and nutrients, in Solanum nigrum L. (S. nigrum)-soil system. Here, S. nigrum were cultivated in heavy metal(loid)s contaminated soils applied with MCNT (100, 500, and 1000 mg kg-1 by concentration, none MCNT addition as control) for 60 days. Our results demonstrated more significant urease, sucrase, and acid phosphatase activities in MCNT than in control soils, which benefit to promoting plant growth. Also, there were significant reductions in available nitrogen and available potassium contents with the treatment of MCNT, while the organic carbon and available phosphorus were not affected by MCNT application. Notably, the alpha diversity of bacterial and fungal communities in the MCNT treatments did not significantly vary relative to control. However, the soil microbial taxonomic compositions were changed under the application of MCNT. Compared to the control, MCNT application increased the relative abundances of the Micrococcaceae family, Solirubrobacteraceae family, and Conexibacter genus, which were positively correlated with plant growth. In addition, the non-metric multidimensional scaling (NMDS) analysis revealed that the community structure of bacterial and fungal communities did not significantly change among all the treatments, and bacterial community structure was significantly correlated with soil organic carbon. At the same time, sucrase activity had the highest relation to fungal community structure. This study highlighted soil microbes have strong resistance and adaptation ability to carbon nanotubes with existence of plants, and revealed linkage between the rhizosphere microenvironment and plant growth, which well improved our understanding of carbon nanotubes in heavy metal phytoremediation.

Targeting Cd coping mechanisms for stress tolerance in Brassica napus under spiked-substrate system: from physiology to remediation perspective.

Cadmium (Cd) is a prevalent, non-essential, carcinogenic, and hazardous heavy metal that reduces plant productivity and capacity of arable land area around the globe. In the present substrate-based pot study, seedlings of Brassica napus 180015 were grown equidistantly in the spiked-substrate medium for 60 days under increasing concentrations of Cd (0, 10, 20, 30, 40, 50 mg kg-1). Following harvest, the morpho-physio-biochemical, antioxidative, and Cd-induced tolerance responses were evaluated in B. napus under an increasing Cd stress regime. Additionally, these parameters were also investigated to select the plant's threshold tolerance limit for Cd under the spiked-substrate system. B. napus showed dynamic behavior regarding morpho-physio-biochemical attributes, including agronomic features, biomass, photosynthetic pigments, relative water content under increased Cd toxicity. Cd stress-induced hydrogen peroxide (H2O2) production with high MDA contents and passive EL, followed by the orchestration of both enzymatic (SOD, POD, APX, CAT, and GR) and non-enzymatic antioxidants (flavonoids, TPC, TPA, proline, and total soluble protein) up to a certain limit. In addition, Cd-induced stress upregulated transcriptional levels of antioxidative enzyme SOD, POD, APX, GR, and MT encoded genes in B. napus. The increasing trend of Cd accumulation in different tissues at the highest Cd concentration was as follows: root > leaf > stem. In spiked substrate system, B. napus demonstrated improved metal extractability performance and a high potential for phyto-management of low to moderate Cd contamination, implying that this study could be used for integrative breeding programs and decontaminating heavy metals in real contaminated scenarios.Novelty statementThis study provides an insight into Cd-coping mechanisms of oilseed rape involved in alleviating toxicity and simultaneous phyto-management of increasing Cd concentration under spiked substrate system. The current study is the first scientific evidence of using a Cd-spiked soilless substrate medium. The present study will further strengthen our understanding of Cd-instigated positive responses in B. napus. Furthermore, it will provide a useful basis for integrative breeding programs and decontaminating heavy metals in real contaminated scenarios.

Experimental Research on the Combustion Characteristics of Transformer Oil Jet Fires in Oil-Filled Equipment under Heat.

Under the action of heat, the jet fire of oil-filled equipment in substations produces intense heat radiation and flame impact, causing serious harm to the personnel and equipment. Therefore, this paper has carried out a series of oil-filled equipment transformer oil jet fire experiments with different nozzle diameters to study the characteristics of transformer oil jet flame combustion. The experiment measured the mass loss rate, axial centerline temperature of the fire plume, and radiant heat flux and processed the flame visualization image. The results show that the combustion process of the transformer oil jet fire can be divided into three stages. The functional relationship between the flame height and mass flow and the functional relationship between the flame temperature and axial height are derived. A prediction model of the radiant heat flux of the transformer oil jet fire for the oil-filled equipment of the substation was established. The research results are of great significance for improving the fire protection design of substations and improving fire management and control capabilities.

Bio-corrosion behavior, antibacterial property and interaction with osteoblast of laserin-situfabricated Ti-Si-Cu coatings on Ti-6Al-4V alloy.

Ti-Si-xCu coatings (TS-xC,x= 5, 10 and 15 wt.%) with advanced bio-corrosion resistance, excellent antibacterial property and biocompatibility were laser cladded on Ti-6Al-4V (TAV) substrate which is widely used as endosseous implants. The bio-corrosion resistance of the TAV substrate was improved due to the presence of Ti5Si3and TiCu phases in the coatings. The addition of Cu in the precursor contributes to the improvement of the antibacterial property of TAV substrate. Meanwhile, induced normal cytoskeleton, well-developed focal adhesion contacts, significant higher cell attachment and proliferation rate were observed for the TS-xC coated samples due to the formation of micro-textured morphology and presence of new phases. The bio-corrosion resistance and antibacterial property depend on Cu content addition in the TS-xC precursor. The results provide a way to fabricate such multiple functional biocoating that would improve the bio-corrosion resistance, antibacterial performance and biocompatibility of TAV.

Small structures with big impact: Multi-walled carbon nanotubes enhanced remediation efficiency in hyperaccumulator Solanum nigrum L. under cadmium and arsenic stress.

With the fast development of nanotechnology, nanomaterials are being increasingly applied for the remediation of contaminated soils. However, few researches have been reported on the complex interactions of carbon nanotubes with heavy metal (loid)s in phytoremediation. Here, we conduct a pot experiment to investigate the effects of multi-walled carbon nanotubes (MWCNTs) on the plant growth and behavior of heavy metal (loid)s in hyperaccumulator-soil system. Cd hyperaccumulator Solanum nigrum L. (S. nigrum) were cultivated in Cadmium (Cd) and Arsenic (As) contaminated soils amended with MWCNTs at 100, 500, and 1000 mg kg-1 for 60 days, respectively. The application of MWCNTs increased the shoot length and plant dry biomass by 5.56%∼25.13% and 5.23%∼27.97%. Whereas, root and leaf growth were inhibited in 1000 mg kg-1 MWCNTs treatments. Meanwhile, MWCNTs at 500 mg kg-1 significantly enhanced the accumulation of heavy metal (loid)s in S. nigrum（18.29% for Cd and 32.47% for As）and alleviated co-contamination induced toxicity, by motivating plant growth, stimulating antioxidant enzymatic activities, and increasing micronutrient content (p < 0.05). The bio-concentration factor of As was decreased (15.31-28.08%) under MWCNTs application, which plays an important role in the alleviation of phytotoxicity. Besides, bioavailable Cd and As were reduced in rhizosphere soils, and the most significant reduction (16.29% for Cd and 8.19% for As) were shown in 500 mg kg-1 MWCNTs treatment. These findings demonstrate that suitable concentration of MWCNTs can enhance remediation efficiency. Our study gives a strong evidence to promote the phytoremediation for co-contaminated soils by using nanomaterials.

Streptomyces griseorubens JSD-1 promotes rice straw composting efficiency in industrial-scale fermenter: Evaluation of change in physicochemical properties and microbial community.

The influence of Streptomyces griseorubens JSD-1 on microbial community succession during rice straw composting in an industrial-scale fermenter was assessed by high-throughput sequencing technology. Compared to uninoculated control, JSD-1 inoculation effectively raised composting temperature and improved other maturation indices. JSD-1 inoculation increased the relative abundance of Actinobacteria in thermophilic phase and Firmicutes in cooling and maturation phases. At the genus level, JSD-1 inoculation increased the abundance of organic matter degrading bacteria (Virgibacillus) and lignocellulose degrading fungi (Chaetomium and Melanocarpus); while it decreased the abundance of pathogenic fungi (Geosmithia and Acremonium). Moreover, JSD-1 changed microbes that differed significantly and altered the key connecting nodes of microbial community. Organic matter and temperature were the most significant indices that had mutual influences on bacterial and fungal communities, respectively. This study demonstrated that JSD-1 was an effective inoculant on rice straw fast composting in the industrial-scale fermenter.

Influence of Cd toxicity on subcellular distribution, chemical forms, and physiological responses of cell wall components towards short-term Cd stress in Solanum nigrum.

Solanum nigrum is a well-documented cadmium (Cd) hyperaccumulator; however, its Cd-induced tolerance capability and detoxification mechanism remain elusive. Hence, a short-term hydroponic experiment was performed in a multiplane glasshouse to determine the influence of Cd toxicity on subcellular distribution, chemical forms, and the physiological responses of cell wall towards Cd stress in a 4-week-old plant. The experiment was conducted following completely randomized design (CRD) with five treatments (n = 4 replicates). The results showed that Cd stress showed dose-dependent response towards growth inhibition. The subcellular distribution of Cd in S. nigrum was in the order of cell wall > soluble fractions > organelles, and Cd was predominantly extracted by 1 M NaCl (29.87~43.66%). The Cd contents in different plant tissues and cell wall components including pectin, hemicellulose 1 (HC1), hemicellulose 2 (HC2), and cellulose were increased with the increase in Cd concentrations; however, the percentage of Cd concentration decreased in pectin and cellulose. Results of the polysaccharide components such as uronic acid, total sugar contents, and pectin methylesterase (PME) activity showed Cd-induced dose-dependent increase relative to exposure Cd stress. The pectin methylesterase (PME) activity was significantly (p < 0.05) enhanced by 125.78% at 75 µM Cd in root, 105.78% and 73.63% at 100 µM Cd in stem and leaf, respectively. In addition, the esterification, amidation, and pectinase treatment of cell wall and Fourier transform infrared spectroscopy (FTIR) assay exhibited many functional groups that were involved in cell wall retention Cd, especially on carboxyl and hydroxyl groups of cell wall components that indicated that the -OH and -COOH groups of S. nigrum cell wall play a crucial role in Cd fixation. In summary, results of the current study will add a novel insight to understand mobilization/immobilization as well as detoxification mechanism of cadmium in S. nigrum.

Two plant growth promoting bacterial Bacillus strains possess different mechanisms in adsorption and resistance to cadmium.

Microorganisms are promising biosorbents for decontaminating cadmium-polluted soil or water systems, but the underlying remediation mechanisms are still unclear. In this study, the cadmium biosorption mechanisms and capabilities of plant growth-promoting microorganisms (Bacillus megaterium NCT-2 and Bacillus paranthracis NT1) were investigated. Batch biosorption experiments showed that the optimal biosorption conditions for B. megaterium NCT-2 and B. paranthracis NT1 were pH 6.0, a biomass dosage of 1.0 g L-1, and an initial Cd2+ concentration of 10 mg L-1, and pH 8.0, a biomass dosage of 1.0 g L-1, and an initial Cd2+ concentration of 10 mg L-1, respectively. The biosorption processes of both biosorbents were well described by the pseudo-second order kinetic model, which indicated that the biosorption of Cd2+ was mainly chemisorption. The intracellular accumulation portion of adsorbed Cd2+ in B. megaterium NCT-2 was much higher than in B. paranthracis NT1 (43.11% and 3.25%, respectively), which resulted in the lower cadmium tolerance (14 mg L-1 and 280 mg L-1, respectively) and higher cadmium removal efficiency (46.79% and 20.45%, respectively) of B. megaterium NCT-2 compared to B. paranthracis NT1. SEM-EDS and FTIR analysis suggested the probable interactions of Cd2+ with the biosorbent surface ligands, such as -OH, -NH, -SO3, CO and -COOH during surface adsorption. Results of qRT-PCR illustrated that the difference in cadmium resistant mechanism and adsorption performance between B. megaterium NCT-2 and B. paranthracis NT1 may be regulated by the genes cadA, zitB, khtT, and bshA and cadA, trkA, czcD, and bshA, respectively. Our results revealed that these two biosorbents have the potential for further use in the development of cadmium remediation technologies and could provide insight into the mechanisms of cadmium biosorption.

Pennisetum giganteum: An emerging salt accumulating/tolerant non-conventional crop for sustainable saline agriculture and simultaneous phytoremediation.

Soil salinity is a global threat to the environmental sustainability, in particular to the developing countries due to their limited resources for soil reclamation. In a greenhouse pot experiment, Pennisetum giganteum, was investigated for its tolerance to salt stress and simultaneous phytoremediation capability. 4 weeks post-germination, NaCl (10, 50, 150, 250, 350, 450 and 550 mM) and tap water (control) was applied after every 2 consecutive days for two weeks in a completely randomized design and their effects were established in the growth and physico-chemical aspects of these plants. Our results indicated that P. giganteum withstood high salt stress (with 550 mM NaCl tolerance threshold level). Interestingly, the plants grown under saline conditions had higher biomass yield when compared to the control. Furthermore, the antioxidant activity and proline content of plants under saline conditions were significantly (p < 0.05) higher than those of control plants, indicating their adaptability to high salt stress. Biochemical analysis such as chlorophyll contents, total soluble sugar, total phenol and protein contents revealed considerable differences between plants grown under higher NaCl stress compared to the control conditions. Additionally, significantly different ionic flux along with high K+/Na+ ratio was observed in plants grown under a range of saline conditions. The results obtained are therefore of value to indicate P. giganteum an eco-friendly alternate source for the phytoremediation of saline soils and may be used as base for future research on this plant. Effective strategies need to be adopted with this plant to reclaim saline-degraded as well as marginal soils.

Plant growth promotion and enhanced uptake of Cd by combinatorial application of Bacillus pumilus and EDTA on Zea mays L.

In developing countries, Cd contamination is ubiquitous which limits agriculture productivity. The current study was designed to investigate the efficacy of plant-Bacillus pumilus-ethylene diamine tetraacetic acid (EDTA) and plant-microbe-chelator (PMC) synergy for enhanced plant growth and Cd-uptake potential of Zea mays in industrially contaminated and cadmium (Cd) spiked soil. A pot experiment was conducted by growing Z. mays seedlings either inoculated with B. pumilus or un-inoculated along with the application of 5 mM EDTA. Plants were exposed to two levels of Cd contamination for 45 days. An increase in Cd uptake was observed in Z. mays inoculated with B. pumilus followed by EDTA treatment as compared to non-inoculated and un-treated ones. Zea mays showed improved values with PMC approach for different growth parameters including root length (41%), shoot length (40%), fresh weight (59%), dry weight (49%), chlorophyll contents (49%), and relative water contents (30%). Higher tolerance index (117%) was observed for plants grown in soil spiked with 300 mg kg-1 Cd (S2). PMC application markedly enhanced Cd uptake potential of Z. mays up to 12% and 68.8%, respectively, in S1 and S2 soil. While the PMC application increased Cd accumulation capacity of Z. mays by 71.2% and 52.5% in S1 and S2 soil. The calculated bioaccumulation and translocation factor revealed that Z. mays possess Cd uptake potential, and this ability can be significantly enhanced with PMC application.

Optimization of NPK fertilization combined with phytoremediation of cadmium contaminated soil by orthogonal experiment.

In the current experiment, influence of NPK composition on the Cd contaminated soil-plant (Solanum nigrum L.) system as well as the phytoremediation efficiency were comprehensively studied. The composition of NPK was optimized for a sustainable phytoremediation and simultaneous agronomic technique in Cd-contaminated soil by orthogonal (L14) experiment, aimed to achieve plant productivity and maximum phytoremediation potential enhancement. Results showed that different treatments of NPK composition enhanced soil properties including saccharase, urease, catalase and acid phosphatase activities as compared to the control treatment, however, soil pH was slightly decreased by 3.64%~6.67% with different composition of NPK treatments. Plant biomass and Cd concentration in the aboveground part (stem and leaves) of S. nigrum were significantly (P < 0.05) enhanced by 14.19%~48.97% and 38.50%~127.15% as compared to control plants with the addition of NPK fertilizers having different composition. Meanwhile, with the application of NPK fertilizer root/shoot Cd ratio and translocation factor (TF) was significantly decreased, however, bioconcentration factor (BCF) was increased as compared to control. Additionally, different composition of NPK fertilizers significantly increased photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids) and soluble protein in comparison to control. The activities of antioxidant enzymes in S. nigrum including ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and glutathione reductase (GR) were increased, while malonaldehyde (MDA) and proline contents were decreased. Principal component analysis (PCA) showed that N3P2K2 treatment had the highest comprehensive score amongst other studied treatments of NPK composition, owing to its optimal composition for the investigated soil-S. nigrum system. Moreover, it was found that optimal composition (N3P2K2) of fertilizer resulted in increase of the plant resistance to Cd and the efficiency of phytoextraction. Therefore, it is suggested to all the small-holder famers and scientific community that precise composition of NPK fertilizer should be utilized according to soil properties, environmental conditions and plant requirements under Cd-stress condition in order to achieve maximum biomass, Cd uptake efficiency as well phytoremediation potential in moderately Cd contaminated soil.