ABSTRACT
A 38-year-old woman experienced a persistent dry cough and progressively worsening dyspnoea for 2 years. Spirometry testing revealed a moderate-to-severe restrictive abnormality. High-resolution chest computed tomography showed diffuse reticulonodular opacities. A lung biopsy disclosed alveolar parenchymal inflammation and fibrosis with bronchiolocentric features, prompting consideration of interstitial pneumonia. Following a thorough investigation of her occupational history and an on-site inspection, it was discovered that the patient had been grinding drill bits designed for printed circuit boards for 8 years, exposing her to hard metals. Mineralogical analyses confirmed excessive tungsten in urine, serum and hair, leading to a diagnosis of hard metal lung disease due to tungsten carbide-cobalt exposure. After discontinuing exposure and commencing corticosteroid therapy, her symptoms, pulmonary function and imaging showed modest improvement. This case highlights the significance of assessing occupational history in patients with interstitial pneumonia and understanding industrial hazards for accurate diagnosis and care.
Subject(s)
Lung Diseases, Interstitial , Occupational Diseases , Occupational Exposure , Humans , Female , Lung Diseases, Interstitial/diagnosis , Lung Diseases, Interstitial/chemically induced , Adult , Occupational Exposure/adverse effects , Occupational Diseases/diagnosis , Tomography, X-Ray Computed , Tungsten/adverse effects , Alloys/adverse effects , Cobalt/adverse effects , Lung/pathology , Lung/diagnostic imagingABSTRACT
Objective: To investigate the correlations between subjective nasal patency, nasal valve area size and aerodynamic parameters in normal nasal cavity by means of numerical simulation, and to explore the effect of nasal valve on nasal subjective sensation and nasal airflow regulation. Methods: A total of 52 healthy participants (31 males and 21 females) with the average age of 37.8 years, were recruited from the outpatient Department of Otorhinolaryngology Head and Neck Surgery, the Ninth People's Hospital Affiliated to the Medical College of Shanghai Jiao Tong University between January and August 2023. Visual Analog Scale (VAS) scores for unilateral nasal subjective sensation were obtained from all participants. Additionally, the aerodynamic characteristics of inspiratory airflow were simulated. A correlation matrix analysis was conducted to identify the correlation strength between these subjective and objective parameters. Results: VAS scores showed negative correlations with unilateral nasal valve cross-sectional area (r=-0.85, P<0.01) and unilateral intranasal airflow (r=-0.57, P<0.01), and was a positive correlation with unilateral nasal resistance (NR) at the front-end of inferior turbinate (r=0.61, P<0.01). The average cross-sectional area of unilateral nasal valve was (0.85±0.35) cm2. The cross-sectional area of unilateral nasal valve was negatively correlated with unilateral NR (r=-0.50, P<0.01), and positively correlated with unilateral nasal airflow (r=0.61, P<0.01). The NR at the nasal valve area accounted for (40.41±23.54)% of the total unilateral NR. Nearly half of the unilateral NR [(46.74±21.38)%] and air warming [(49.96±10.02)%] occurring before the front end of inferior turbinate were achieved. Conclusions: The nasal valve area plays a crucial role in influencing nasal NR, unilateral nasal airflow, and changes in nasal airflow temperature. Moreover, it is associated with subjective perception of nasal patency.
Subject(s)
Nasal Obstruction , Male , Female , Humans , Adult , Nasal Obstruction/surgery , Correlation of Data , China , Nasal Cavity , TurbinatesABSTRACT
BACKGROUND: The importance of early intravenous (IV) antibiotic use for Mycobacterium abscessus complex lung diseases (MABC-LD) treatment remains unknown. METHODS: A retrospective multi-centre observational study was conducted in Taiwan. Patients who were diagnosed with and received treatment for MABC-LD from January 2007 to April 2021 were included. Treatment outcome was defined as modified microbiological cure of MABC-LD.RESULTS: Of the 89 enrolled patients, 34 (38.2%) received IV antibiotics as part of the treatment regimen. The median time to IV initiation was 1 day (IQR 1???49); 24 (70.6%) of these patients received IV agents within 4 weeks, defined as early-use. Forty-two (47.2%) patients achieved modified microbiological cure. In the multivariable logistic analysis, early IV antibiotic use was an independent factor associated with modified microbiological cure (aOR 5.32, 95% CI 1.66???17.00), whereas high radiological score (aOR 0.86, 95% CI 0.73???1.00) demonstrated negative association.CONCLUSIONS: In the present study, early use of effective IV antibiotic was prescribed in a low percentage (27%) for MABC-LD. By contrast, early IV antibiotic use was correlated with higher microbiological cure than were late or non-use. Future larger and prospective studies are needed to validate the association.
Subject(s)
Lung Diseases , Mycobacterium Infections, Nontuberculous , Mycobacterium abscessus , Humans , Anti-Bacterial Agents/therapeutic use , Mycobacterium Infections, Nontuberculous/drug therapy , Mycobacterium Infections, Nontuberculous/microbiology , Lung Diseases/drug therapy , Lung Diseases/microbiology , Prospective Studies , Retrospective StudiesABSTRACT
Objective: To identify the internal nasal valve (INV) and to evaluate its key parameters in the established 3D models of nasal cavity space via Mimics from CT images, in order to provide evidence for quantitative diagnosis of nasal valve compromise. Methods: A total of 32 Han adults without nasal diseases who underwent maxillofacial CT test in Shanghai Ninth People's Hospital from January 2015 to December 2018 were retrospectively recruited, including 16 males and 16 females, with the age ranged from 20 to 80 years (50% age<50 years old). Maxillofacial CT images were used to create 3D model of nasal cavity space. The INV was identified and the following parameters were measured: the angle between the INV and the nasal bone (θINV-B), unilateral cross-sectional area of the INV (AINV-R, AINV-L), total cross-sectional area of the INV (AINV), unilateral height of the INV (HINV-R, HINV-L), unilateral nasal valve angle (αINV-R, αINV-L), and the sum of nasal valve angle (αINV). The AINV in our study was compared with the results of the previously adopted planes (PlaneC, perpendicular to the hard palate and PlaneB, plane perpendicular to the nasal bone). The parameters above were compared among genders, age and race groups. SPSS 26 and GraphPad Prism 9 software were used for statistical analysis and mapping of data. Results: The AINV in our study was (214.87±52.94) mm², which was significantly less than that of PlaneC (254.97±47.80) mm² and PlaneB (226.07±57.36) mm². The measured parameters were as follows: θINV-B was (82.07±7.06)°; AINV-R was (112.66±31.39) mm²; AINV-L was (102.21±27.14) mm²; AINV was (214.87±52.94) mm²; HINV-R was (24.87±4.62) mm; HINV-L was (24.35±4.86) mm; αINV-R was (20.48±2.99)°; αINV-L was (19.65±3.82)°; αINV was (40.13±6.24)°. The AINV-R was larger than AINV-L (t=2.33, P<0.05); The HINV, AINV-R, AINV-L and AINV of males were more than those of females (t value was 5.77, 3.21, 2.91 and 3.52, respectively, all P<0.01). The AINV of the young group (<50 years) was larger than that of the old group (t=2.83, P<0.01); The θINV-B was different between the Han people and the Caucasian (t=2.92,P<0.01). The αINV of the Han people was larger than that of Caucasians (Z=-6.92, P<0.01), but the HINV was smaller (Z=-3.89, P<0.01). Conclusion: The AINV carried out in 3D models of nasal cavity space is significantly smaller than that obtained by the previous methods of CT evaluation. INV static parameters differ among genders, age and race groups.
Subject(s)
Nasal Cavity , Nose , Adult , Female , Humans , Male , Young Adult , Middle Aged , Aged , Aged, 80 and over , Nasal Cavity/diagnostic imaging , Retrospective Studies , China , Nasal BoneABSTRACT
Objective: To evaluate the functional changes of lateral pterygoid muscle (LPM) in the patients with temporomandibular disorders (TMD) using histogram texture analysis. Methods: A total of 28 TMD patients, including 15 males and 13 females, aged (26.2±12.1) years (15-62 years), were enrolled in the outpatient clinic of Department of Radiology, Hainan Hospital of Chinese PLA General Hospital from May 2016 to April 2020. At the same time, 13 volunteers with normal temporomandibular joint [6 males and 7 females, aged (23.5±3.3) years (20-30) years] were recruited. All participants were performed with temporomandibular joint MRI scan, and the subjects were classified into 3 groups according to the disc displacement: TMD with disc without displacement (TMD-DwoD), TMD with disc displacement without reduction (TMD-DDwoR) and NC with disc displacement without reduction (NC-DwoD) for each temporomandibular joint. The area, signal intensity, coefficient of variation (CV) of mean signal intensity and histogram texture of the superior belly of LPM (SBLPM) and inferior belly of LPM (IBLPM) were measured using regions of interests. Results: The area of SILPM presented significantly smaller in TMD-DwoD [(67.36±30.23) mm2] compared with that in NC-DwoD [(91.42±45.01) mm2] (P<0.05) and in TMD-DDwoR group [(72.27±37.53) mm2] (P>0.05). The mean signal intensity of SBLPM was identified significantly higher in TMD-DwoD (304.20±29.90) and TMD-DDwoR (315.06±40.20) compared with that in NC-DwoD (269.79±25.54) (P<0.05). The CV of mean signal intensity of SBLPM presented significantly higher in TMD-DwoD (8.89±2.60) compared with that in NC-DwoD (6.76±1.82) and TMD-DDwoR (7.63±1.62) (P<0.05). The skew and kurtosis showed no significantly differences among three groups for SBLPM (P>0.05). The mean signal intensity and CV for IBLPM presented significantly higher in TMD-DwoD (286.69±23.48 and 9.96±2.24, respectively) and TMD-DDwoR (275.98±30.55 and 10.25±1.96, respectively) compared with that in NC-DwoD (243.56±17.04 and 8.49±1.94, respectively) (P<0.05). The skewness showed significantly lower in TMD-DwoD (0.90±0.94) and TMD-DDwoR (0.73±0.97) compared with that in NC-DwoD (1.40±1.03) (P<0.05). The area and kurtosis for IBLPM showed no significant differences among three groups (P>0.05). Conclusions: The MR histogram texture analysis could primarily be considered as a tool to evaluate the LPM function in TMD patients.
Subject(s)
Joint Dislocations , Temporomandibular Joint Disorders , Adolescent , Adult , Female , Humans , Magnetic Resonance Imaging , Male , Pterygoid Muscles/diagnostic imaging , Temporomandibular Joint , Temporomandibular Joint Disc , Temporomandibular Joint Disorders/diagnostic imaging , Young AdultABSTRACT
Objective: To evaluate the histological changes of the retrodiscal tissue in the patients with temporomandibular disorders (TMD). Methods: Thirty-three TMD patients were performed with temporomandibular joint (TMJ) MRI examinations from April 2018 to April 2020 in TMD clinic, Hainan Hospital of General Hospital of Chinese PLA. The patients groups were classified according to the disc position as follows: normal position (NP) (29 TMJ), disk displacement with reduction (DDwR) (14 TMJ) and disk displacement without reduction (DDwoR) (23 TMJ). The gray-level co-occurrence matrix (GLCM) analysis was applied with the retrodiscal tissue on the open oblique sagittal proton weighted images. The texture parameters included as follows: angular second moment (ASM), contrast, correlation, inverse difference moment (IDM) and entropy. The statistical methods mainly included Kruskal-Wallis H test, one-way analysis of variance and receiver operating characteristic curve (ROC), to analyze the texture characteristic parameters of the retrodiscal tissue are statistically significant. Results: There was no significant difference of the retrodiscal tissue's ASM, contrast, correlation and IDM among 3 groups (P>0.05). The entropy in NP group (3.98±0.44) and DDwR group (4.20±0.52) presented significant higher than that in DDwoR group (3.70±0.38) (P<0.05). ROC analysis for the entropy demonstrated that the area under the curve was 0.70 when differentiating NP and DDwR (cut-off value 0.38, sensitivity 82.61%, specificity 55.17%), and was 0.79 when differentiating DDwR and DDwoR (cut-off value 0.47, sensitivity 82.61%, specificity 64.29%). Conclusions: Texture entropy could be primarily used to evaluate the histological and pathological changes of the retrodiscal tissue in the TMD patients.
Subject(s)
Joint Dislocations/diagnostic imaging , Temporomandibular Joint Disorders/diagnostic imaging , Humans , Magnetic Resonance Imaging , Pilot Projects , Temporomandibular Joint , Temporomandibular Joint Disc/diagnostic imagingSubject(s)
Fractures, Bone/diagnosis , Fractures, Bone/therapy , Nasal Bone/pathology , China , HumansABSTRACT
Objective: To investigate the application and clinical outcomes of using 3D computer-assisted printing technique combined with plastic titanium mesh in the reconstruction of maxillary defect. Methods: Clinical data of 14 patients in Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Ninth People's Hospital from January 2016 to June 2018, who were treated for partial or total removal of the maxilla due to benign or malignant tumors and those acquired maxillary defects caused by severe compound trauma were analyzed retrospectively. Twelve males and 2 females were included, with the age ranging from 16 to 51 years old. The sinonasal malignant tumors included squamous carcinoma (n=2) while benign tumors included hemangioma (n=1), maxillary fibrous dysplasia (n=3), maxillary cyst (n=2) and giant cell tumor of the maxilla (n=1). Five cases of post-traumatic maxillary defect were also included. According to preoperative thin-layer CT scanning data, computer modeling data was transmitted to a 3D printer to print out the original model and the reconstructed model. Preoperative simulation of tumor removal and maxillary reconstruction was done on the patient's original model, and the titanium mesh was shaped on the reconstructed model in order to properly reconstruct the area needed to be repaired. The pre-made titanium mesh was implanted into the defect area, the soft tissue flaps were reset, layered stitching and the local pressured bandage were used after surgery. Through postoperative clinical and CT examination, the patient's maxillofacial shape, nasal function and complications were evaluated. The results were analyzed by descriptive statistical method. Results: Lesions could be completely removed within the predicted range on the preoperative 3D-printed models of all cases. After debridement, titanium mesh could be implanted easily without re-shaping and trimming during surgery as in trauma cases. Titanium mesh could completely cover the missing bone surface closely, with titanium nails fixed smoothly, and the implanted titanium mesh was solid and stable. After the follow-up of 6 to 20 months, all patients were satisfied with the facial symmetry and the function was recovered well. Conclusion: Using 3D computer-assisted printing technique combined with plastic titanium mesh in the reconstruction of maxillary defect can accurately restore the maxillary structure for soft tissue support, and restore the facial shape and function.
Subject(s)
Computer-Aided Design , Maxilla/surgery , Plastic Surgery Procedures , Printing, Three-Dimensional , Surgical Mesh , Adolescent , Adult , China , Female , Humans , Male , Maxilla/pathology , Maxillary Neoplasms/surgery , Middle Aged , Plastics , Retrospective Studies , Titanium , Young AdultABSTRACT
Objective: To investigate the effect of nasal septum perforation (SP) with different locations and sizes on nasal airflow by means of numerical simulation. Methods: Two healthy persons with normal nasal anatomy were enrolled in this study, including a 45 years old male (case 1) and a 36 years old female (case 2). Nasal CT data was used as the basis to create nasal airway numerical models of nasal SP with different locations (anterior caudal, central caudal, posterior caudal and anterior cranial) and sizes (diameter of 10 mm and 5 mm respectively). The inspiratory airflow characteristics (nasal cavity volume, nasal cavity wall area, pressure, nasal resistance, temperature, airflow velocity, wall shear stress, airflow-rate partitioning and vortex) of these nasal airway numerical models were simulated and analyzed. Pearson correlation analysis was performed between nasal resistances, airflow temperature and nasal cavity wall area. Results: In terms of pressure and nose resistance, the anterior caudal and larger size SP lead to more obvious variation of pressure distribution, and increased nasal resistance was especially found in the nasal cavity with anterior and medium caudal SP. In terms of temperature, the anterior (caudal and cranial) and larger size SP had significant effect on local temperature gradient as same as the anterior cranial and smaller size SP. Nasal heating efficiency was lower in nasal model with the anterior and larger size SP than that in the normal model. The temperature difference from the nostril to the end of nasal septum had positive correlation with nasal cavity wall area (R(2) value of case 1 and case 2 was 0.69, 0.41, respectively, all P<0.01). In terms of airflow velocity, the anterior caudal and cranial SP had more significant effect on the average airflow velocity in the nasal cavity. The anterior and medium caudal SP could make the airflow distribution in the asymmetric bilateral nasal cavity more unbalanced compared to the bilateral symmetrical nasal models. The anterior and medium SP resulted in a more pronounced vortex distribution than the posterior SP. Conclusions: The effect of SP on nasal cavity is related to its location and size. The anterior and larger size SP shows more negative influence on intranasal pressure, nasal resistance, heat transmission efficiency, airflow-rate partitioning than the posterior and smaller size SP.
Subject(s)
Computer Simulation , Models, Biological , Nasal Cavity , Nasal Septal Perforation/pathology , Adult , Female , Humans , Inhalation , Male , Middle Aged , Nasal Septum , Numerical Analysis, Computer-Assisted , TemperatureABSTRACT
Objective: To investigate the clinical effect of the flap on post aurem and mastoid region on immediate repair of part helix defect. Method: Fifteen patients with part helix defect were immediately repaired using the flap on post aurem and mastoid region. Firstly, the skin tissue above the defect of the helix was used as the pedicle. The flap was drawn toward the post aurem and mastoid region closing to the auriculocephalic angle. The flap was generally 3-4 cm long and at least 1 cm wide. Secondly, the flap was lifted on the perichondrium, and the structure of the helix was formed by upward rotation of the flap to repair the defect of the helix. Finally, the flap was sutured to repair the helix defect. Result: Fifteen cases were applied with this method to repair immediately the defect in the emergency environment. The sizes of helix contour were satisfactory and the auriculocephalic angles had not changed. The ear shape was stable after 1 to 3 months of followîup. Conclusion: The flap on post aurem and mastoid region could immediately repair the part helix defect. It has the advantages of simple operation, high flap survival rate and one-time satisfactory shape.î.
Subject(s)
Plastic Surgery Procedures , Skin Transplantation , Surgical Flaps , Cartilage , Humans , MastoidABSTRACT
Objective:To investigate the pre- and post-operative intranasal airflow characteristics on nasal septum deviation and the correlation between subjective nasal patency and airflow characteristics by numerical simulation. Method:Forty-two nasal septum deviation cases were enrolled in this study. Pre- and post-operative VAS score of subjective nasal patency were recorded. We created computational fluid dynamics (CFD) nasal cavity airway models based on computed tomography and investigated the pre- and post-operative intranasal airflow characteristics. Result:Postoperative subjective nasal patency on patients with septal deviation showed significant improvement than preoperative (P<0.01). The intranasal airflow on deviated side nasal cavity increased from preoperative (34.94±14.82)% to postoperative (47.67±3.58)% (P<0.01). The parameters of ΔPï¼anterior inferior turbinateï¼,ΔTï¼anterior inferior turbinateï¼ and ΔTï¼posterior end of the septumï¼decreased significantly from preoperative (7.657±4.839)Pa to postoperative (4.500±2.403)Paï¼preoperative (8.482±1.767)K to postoperative ï¼7.096±1.127ï¼Kï¼and preoperative (11.386±0.505ï¼K to postoperative ï¼10.895±0.736ï¼K respectively î(P<0.01).î The value of NR1 decreased from (0.043±0.021) Paâ¢s/ml to (0.038 0±0.016) Paâ¢s/ml, showed statistically significant difference (P<0.05), but the value of NR2 decreased from (0.071 0±0.035)îPaâ¢s/mlî to (0.061 5±0.048) Paâ¢s/ml, showed no statistically significant difference (P>0.05). The related degree of subjective nasal patency VAS score on ΔTï¼anterior inferior turbinateï¼ and intranasal airflow of deviated side nasal cavity are higher (R²=0.619 6 and îR²=0.508 7ï¼than ΔPï¼anterior inferior turbinateï¼and NR2ï¼R²=0.326 7 and îR²=0.298 1ï¼.It suggested that there is no correlation between subjective nasal patency VAS score and ΔTï¼posterior end of the septumï¼ï¼NR1 (R²=0.0451 and îR²=0.0721). Conclusion:It showed different pressure and temperature gradient in bilateral nasal cavity of nasal septal deviation. The septoplasty improved the patient's feeling of subjective nasal patency, and recovered the balance of the airflow distribution on bilateral nasal cavity. By correlation analysis, we concluded that nasal resistance could not represent the severity of subjective nasal patency, but the intranasal airflow showed good correlations with subjective nasal patency, especially the temperature drop in mucous membrane at the nasal front end is more sensitive to reflect subjective nasal patency feeling.
ABSTRACT
Objective: To investigate the feasibility and clinical effect of reconstruction the ear using cartilage from amputated ear. Methods: 30 patients (22 males, 8 females, age 22-50, 18 cases right ear, 12 cases left ear) with partial auricles defects received replantation of amputated ears using the cartilages from the amputated ears from January, 2013 to June, 2017.Firstly, an advancements postauricular skin flap was made.Secondly, the skin of the amputated ear was removed and its cartilage was retained, then the cartilage was sutured in situ to form cartilage bracket.Finally, the postauricular crimp scalp flap was sutured to form the helix structure.After three months, the back of the reconstructive ear was repaired with the full thickness graft to restore the cranio-auricular angle. Results: Thirty cases were applied with this method to repair the defects.The sizes of auricle contour were good, and the cranio-auricular angles were restored after skin-grafting.The ear shape was stable in 3-6 months' follow-up.VAS was used to evaluate patients satisfaction.All parameters before and after the procedure had significant difference (P<0.05). Conclusions: Patients with partial auricles defects can receive replantation of amputated ears using the cartilages from the amputated ears which has the advantage of short treatment period, satisfactory clinical effect and avoidance using the costal cartilages.Its long-term effect is stable.
Subject(s)
Amputation, Traumatic/surgery , Dermatologic Surgical Procedures/methods , Ear Auricle/injuries , Ear Cartilage/surgery , Replantation/methods , Surgical Flaps , Adult , Feasibility Studies , Female , Humans , Male , Middle Aged , Young AdultABSTRACT
Objective: To investigate the potential effect of proteoglycans (PG) and glycosaminoglycans (GAG) on the stability of resin-dentin bonds against artificial saliva storage. Methods: Seventy-two extracted molars were used to obtain standard dentin bonding surface, and the specimens were etched for 15 s with 37% phosphoric acid and divided into three groups using a table of random number. Then the three groups undergone different incubating procedures as follow: specimens in chondroitinase ABC (C-ABC) group were incubated with C-ABC, specimens in trypsin (TRY) group were incubated with trypsin, and specimens in the control group were incubated with deionized water. All specimens were incubated at 37 â for 48 h in the oscillators. Then specimens in each group were randomly assigned into three subgroups (n=8) as follows: immediate control subgroup, aging subgroups with artificial saliva storage for 6 months and 12 months. Microtensile bond strength (µTBS), fracture mode, bonding interface morphology and nanoleakage were evaluated. Results: Immediately and with artificial saliva storage for 6 months and 12 mouths, the µTBS of TRY group ([49.04±3.57], [37.01±3.21] and [35.27±3.56] MPa) were significantly higher than those in the control group ([40.71±3.32], [28.87±2.34] and [24.20±2.07] MPa) (P<0.05). The immediate µTBS of C-ABC group ([32.94±2.45] MPa) was significantly lower than that of the control group (P<0.05). While with artificial saliva storage for 6 months and 12 mouths, the µTBS of C-ABC group ([26.46±2.45] and [22.50±2.58] MPa) were no differences with those of the control group (P>0.05). The ratio of cohesive fracture increased with the extension of aging time. Some narrow gaps were found in hybrid layer of the control group with artificial saliva storage for 6 months and 12 mouths. Conclusions: Removal of PG increased the µTBS and durable bonds to dentin, while removal of GAG decreased the µTBS, however, it can be of help to create more durable bonds to dentin.
Subject(s)
Dental Bonding , Dentin/chemistry , Glycosaminoglycans/pharmacology , Proteoglycans/pharmacology , Saliva, Artificial , Dental Etching , Dental Materials , Dentin-Bonding Agents , Materials Testing , Molar , Phosphoric Acids , Random Allocation , Resin Cements , Resins, Synthetic , Tensile Strength/drug effectsABSTRACT
OBJECTIVES: Osteoarthritis (OA) is the most common form of arthritis, affecting approximately 15% of the human population. Recently, increased concentration of nitric oxide in serum and synovial fluid in patients with OA has been observed. However, the exact role of nitric oxide in the initiation of OA has not been elucidated. The aim of the present study was to investigate the role of nitric oxide in innate immune regulation during OA initiation in rats. METHODS: Rat OA was induced by performing meniscectomy surgery while cartilage samples were collected 0, 7, and 14 days after surgery. Cartilage cytokine levels were determined by using enzyme-linked immunosorbent assay, while other proteins were assessed by using Western blot RESULTS: In the time course of the study, nitric oxide was increased seven and 14 days after OA induction. Pro-inflammatory cytokines including tumour necrosis factor (TNF)-α, interleukin (IL)-1ß, and IL-6 were decreased. L-NG-Nitroarginine methyl ester (L-NAME, a non-specific nitric oxide synthase inhibitor) significantly decreased cartilage nitric oxide and blocked immune suppression. Further, L-NAME decreased Matrix metalloproteinase (MMPs) and increased tissue inhibitor of metalloproteinase (TIMP) expression in meniscectomised rats. CONCLUSION: Nitric oxide-dependent innate immune suppression protects cartilage from damage in the early stages of OA initiation in rats.Cite this article: C-C. Hsu, C-L. Lin, I-M. Jou, P-H. Wang, J-S. Lee. The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in ca rtilage da mage. Bone Joint Res 2017;6:253-258. DOI: 10.1302/2046-3758.64.BJJ-2016-0161.R1.
ABSTRACT
Auricularis keloids are the keloids locating on the earlobe and helix which often caused by ear piercing, burn wound and skin injury. Because of its high recurrence, the treatment of auricularis keloids is troublesome to ENT doctors. In the past, clinicians harvested some achievements by combining surgical therapy, drug therapy and physical therapy. Keloid therapies were reviewed in this article.
Subject(s)
Ear Auricle/surgery , Ear Diseases/surgery , Keloid/surgery , Combined Modality Therapy/methods , Ear Diseases/therapy , Ear, External , Humans , RecurrenceABSTRACT
The diffusion of ligands to actives sites of proteins is essential to enzyme catalysis and many cellular signaling processes. In this contribution we review our recently developed methodology for calculation of rate constants for diffusion and binding of small molecules to buried protein active sites. The diffusive dynamics of the ligand obtained from molecular dynamics simulation is coarse grained and described by a Markov state model. Diffusion and binding rate constants are then obtained either from the reactive flux formalism or by fitting the time-dependent population of the Markov state model to a phenomenological rate law. The method is illustrated by applications to diffusion of substrate and inhibitors in [NiFe] hydrogenase, CO-dehydrogenase, and myoglobin. We also discuss a recently developed sensitivity analysis that allows one to identify hot spots in proteins, where mutations are expected to have the strongest effects on ligand diffusion rates.