Electrokinetic particle trapping in microfluidic wells using conductive nanofiber mats.

The frequency dependence of electrokinetic particle trapping using large-area (>mm2) conductive carbon nanofiber (CNF) mat electrodes is investigated. The fibers provide nanoscale geometric features for the generation of high electric field gradients, which is necessary for particle trapping via dielectrophoresis (DEP). A device was fabricated with an array of microfluidic wells for repeated experiments; each well included a CNF mat electrode opposing an aluminum electrode. Fluorescent microspheres (1 µm) were trapped at various electric field frequencies between 30 kHz and 1 MHz. Digital images of each well were analyzed to quantify particle trapping. DEP trapping by the CNF mats was greater at all tested frequencies than that of the control of no applied field, and the greatest trapping was observed at a frequency of 600 kHz, where electrothermal flow is more significantly weakened than DEP. Theoretical analysis and measured impedance spectra indicate that this result was due to a combination of the frequency dependence of DEP and capacitive behavior of the well-based device.

An electrospun nanofiber mat as an electrode for AC-dielectrophoretic trapping of nanoparticles.

In order to trap nanoparticles with dielectrophoresis, high electric field gradients are needed. Here we created large area (>mm2) conductive carbon nanofiber mats to trap nanoparticles with dielectrophoresis. The electrospun fiber mats had an average diameter of 267 ± 94 nm and a conductivity of 2.55 S cm-1. Relative to cleanroom procedures, this procedure is less expensive in creating bulk conductive nanoscale features. The electrospun fiber mat was used as one electrode, with an indium-tin-oxide glass slide serving as the other (separated approximately 150 µm). Numerical models showed that conductive nanoscale fibers can generate significant field gradients sufficient to overcome Brownian transport of nanoparticles. Our experiments trapped 20 nm fluorescent polystyrene beads at 7 Vrms and 1 kHz. Trapping is further enhanced through simultaneous electrohydrodynamic motion. Overall, this straightforward electrospun fiber mat can serve as a foundation for future use in microscale electrokinetic devices.