ABSTRACT
Quinoxalines are a class of veterinary drugs with antibacterial and growth-promoting functions. They are often widely used to treat and prevent animal diseases and are illegally used as animal growth promoters to increase economic benefits. Quinoxalines could be easily metabolized in animals to various residue markers and remain in animal-derived foods, which would pose a serious threat to human health. Consequently, it is necessary to detect the residues of quinoxalines and their metabolites. This article reviewed and evaluated immunoassays for quinoxalines and their metabolites in animal-derived foods, mainly including enzyme-linked immunosorbent assays, fluorescence immunosorbent assays, immunochromatography, and surface plasmon resonance biosensors. In addition, we deeply explored the design of haptens for quinoxalines and their metabolites and analyzed the effect of haptens on antibody performance. This paper aims to provide guidance and references for their accurate and sensitive detection, thereby ensuring food safety and human public health.
Subject(s)
Antibodies , Quinoxalines , Animals , Humans , Quinoxalines/analysis , Enzyme-Linked Immunosorbent Assay/methods , Immunoassay , Haptens/chemistryABSTRACT
Ochratoxin A (OTA), a dangerous mycotoxin, is found in many crops. It is essential to create sensitive OTA detection techniques to ensure food safety. Based on the principle of p-nitrophenol (PNP) quenched the fluorescence of bovine serum albumin silver nanocluster (BSA-AgNCs) through an internal filtering effect, and phosphate activated fluorescence of calcein-Ce3+ system, a ratiometric fluorescence immunoassay for OTA detection was developed. In this strategy, the value of F518/F640 was used as a signal for response of OTA concentration. The detection range of this strategy was 0.625-25 ng/mL, the limit of detection (LOD) was 0.04 ng/mL. This new immunoassay offered a brand-new platform for detecting OTA.
Subject(s)
Biosensing Techniques , Ochratoxins , Silver , Ochratoxins/analysis , Immunoassay/methods , Limit of Detection , Biosensing Techniques/methodsABSTRACT
In this study, an alkaline phosphatase (ALP)-mediated fluorescence immunoassay for detecting zearalenone (ZEN) was established based on the oxVB1 fluorescence signal modulated by MnO2 nanosheets (MnO2 NS). As the ALP-antibody content increased, more 2-phosphoascorbic acid (AAP) was hydrolyzed to ascorbic acid (AA) which destroyed the MnO2 NS rapidly. In the lack of MnO2 NS, VB1 cannot be oxidized to oxVB1 for emitting fluorescence. On the contrary, the fluorescence of oxVB1 recovered slowly with the decrease of the ALP-antibody concentration. In the optimization condition, the detection limit of this method was 15.5 pg mL-1. Moreover, the recovery of ZEN in real samples ranged from 94.24 % to 108.26 %, which indicated the remarkable accuracy and reliability of this approach. Meanwhile, the proposal of this fluorescence immunoassay provided a new possibility for detecting other targets by replacing antibodies and antigens.
Subject(s)
Manganese Compounds , Zearalenone , Oxides , Fluorescence , Reproducibility of Results , Alkaline Phosphatase , Immunoassay , Coloring Agents , Limit of DetectionABSTRACT
Ochratoxin A (OTA) is one of the most prevalent and toxic mycotoxins. Ultrasensitive and convenient detection of OTA is urgent demanded for public health. In this work, a dual-readout immunoassay was established for the detection of OTA based on Ce4+ oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) and Ce3+ inducing aggregation induced emission (AIE) of Au nanoclusters (AuNCs). Under alkaline phosphatase (ALP), the ascorbic acid 2-phosphate (AA2P) can form ascorbic acid (AA) by dephosphorylation. The AA can reduce Ce4+ to generate Ce3+, which induced the AIE of AuNCs to enhance the fluorescence intensity of AuNCs. Meanwhile, unreacted Ce4+ oxidized TMB to form blue oxTMB. Thus, a dual-readout immunoassay was developed based on AIE of AuNCs and TMB as substrate. The limits of detection (LODs) were as low as 0.62 ng/mL for fluorescent assay and 0.81 ng/mL for colorimetric assay. The recoveries of OTA from corn were 94.4%-107.7% for the fluorescent mode and 93.7%-106.9% for the colorimetric mode. The results verified that the cerium ions triggered dual-readout immunoassay was reliable to sensitive detect OTA in corn samples.
Subject(s)
Cerium , Ochratoxins , Alkaline Phosphatase , Ascorbic Acid , Benzidines , Colorimetry/methods , Coloring Agents , Immunoassay , Ions , Limit of Detection , Ochratoxins/analysisABSTRACT
Influenza virus infects the host and transmits through the respiratory tract (i.e., the mouth and nose); therefore, the development of intranasal influenza vaccines that mimic the natural infection, coupled with an efficient mucosal adjuvant, is an attractive alternative to current parenteral vaccines. However, with the withdrawal of cholera toxin and Escherichia coli heat-labile endotoxin from clinical use due to side effects, there are no approved adjuvants for intranasal vaccines. Therefore, safe and effective mucosal adjuvants are urgently needed. Previously, we reported that one derivative of α-Galactosylceramide (α-GalCer), 7DW8-5, could enhance the protective efficacy of split influenza vaccine by injection administration. However, the mucosal adjuvanticity of 7DW8-5 is still unclear. In this study, we found that 7DW8-5 promotes the production of secret IgA antibodies and IgG antibodies and enhances the protective efficacy of the split influenza vaccine by intranasal administration. Furthermore, co-administration of 7DW8-5 with the split influenza vaccine significantly reduces the virus shedding in the upper and lower respiratory tract after lethal challenge. Our results demonstrate that 7DW8-5 is a novel mucosal adjuvant for the split influenza vaccine.
Subject(s)
Influenza Vaccines , Influenza, Human , Orthomyxoviridae Infections , Adjuvants, Immunologic , Administration, Intranasal , Animals , Antibodies, Viral , Galactosylceramides , Glycolipids , Humans , Immunity, Mucosal , Influenza, Human/prevention & control , Mice , Mice, Inbred BALB C , Orthomyxoviridae Infections/prevention & control , Vaccines, InactivatedABSTRACT
Influenza virus only encodes a dozen of viral proteins, which need to use host machinery to complete the viral life cycle. Previously, KAP1 was identified as one host protein that potentially interacts with influenza viral proteins in HEK 293 cells. However, the role of KAP1 in influenza virus replication in human lung alveolar epithelial cells and the underlying mechanism remains unclear. In this study, we first generated KAP1 KO A549 cells by CRISPR/Cas9 gene editing. KAP1 deletion had no significant effect on the cell viability and lack of KAP1 expression significantly reduced the influenza A virus replication. Moreover, we demonstrated that KAP1 is involved in the influenza virus entry, transcription/replication of viral genome, and viral protein synthesis in human lung epithelial cells and confirmed that KAP1 interacted with PB2 and NS1 viral proteins during the virus infection. Further study showed that KAP1 inhibited the production of type I IFN and overexpression of KAP1 significantly reduced the IFN-ß production. In addition, influenza virus infection induces the deSUMOylation and enhanced phosphorylation of KAP1. Our results suggested that KAP1 is required for the replication of influenza A virus and mediates the replication of influenza A virus by facilitating viral infectivity and synthesis of viral proteins, enhancing viral polymerase activity, and inhibiting the type I IFN production.
Subject(s)
Influenza A virus , Influenza, Human , Epithelial Cells , HEK293 Cells , Humans , Influenza A virus/genetics , Lung , Viral Nonstructural Proteins/genetics , Viral Nonstructural Proteins/metabolism , Virus Replication/geneticsABSTRACT
Apatite fission-track (AFT) analysis, a widely used low-temperature thermochronology method, can provide details of the hydrocarbon generation history of source rocks for use in hydrocarbon exploration. The AFT method is based on the annealing behavior of fission tracks generated by 238U fission in apatite particles during geological history. Due to the cumbersome experimental steps and high expense, it is imperative to find an efficient and inexpensive technique to determinate the annealing degree of AFT. In this study, on the basis of the ellipsoid configuration of tracks, the track volume fraction model (TVFM) is established and the fission-track volume index is proposed. Furthermore, terahertz time domain spectroscopy (THz-TDS) is used for the first time to identify the variation of the AFT annealing degree of Durango apatite particles heated at 20, 275, 300, 325, 450, and 500 â for 10 h. The THz absorbance of the sample increases with the degree of annealing. In addition, the THz absorption index is exponentially related to annealing temperature and can be used to characterize the fission-track volume index. Terahertz time domain spectroscopy can be an ancillary technique for AFT thermochronological research. More work is urgently needed to extrapolate experimental data to geological conditions.
ABSTRACT
In this paper, terahertz time-domain spectroscopy was employed to study the properties of marine kerogen from Western Canada Basin at various temperatures. On the basis of terahertz absorption coefficients of samples, the evolution model of oil and gas generation in kerogen was established, explained, and verified by IR and principal component analysis. In addition, the molecular models of kerogen were simulated by means of the quantum chemistry. Then the vibration characteristics of functional groups in terahertz band were analyzed, and the reasons for different absorptions of kerogen at different temperatures were explained. This study will provide a reference for thermal evolution kinetics of kerogen, as well as an effective complement to the potential evaluation of oil and gas resources.
ABSTRACT
The flow-pattern transition has been a challenging problem in two-phase flow system. We propose the terahertz time-domain spectroscopy (THz-TDS) to investigate the behavior underlying oil-water flow in rectangular horizontal pipes. The low water content (0.03-2.3%) in oil-water flow can be measured accurately and reliably from the relationship between THz peak amplitude and water volume fraction. In addition, we obtain the flow pattern transition boundaries in terms of flow rates. The critical flow rate Qc of the flow pattern transitions decreases from 0.32 m3 h to 0.18 m3 h when the corresponding water content increases from 0.03% to 2.3%. These properties render THz-TDS particularly powerful technology for investigating a horizontal oil-water two-phase flow system.