Investigations of pathological immunohistochemical and immunocytochemical findings in natural infection with Mycoplasma gallisepticum in laying hens

Background: Mycoplasmosis is an infectious disease caused by Mycoplasma gallisepticum (MG), usually seen in therespiratory system of chickens, chick and turkeys, that causing great economic loss. The disease is characterized by respiratory system lesions such as sinusitis, tracheitis, airsacculitis, pneumonia and other symptoms such as loss of yield, arthritis,tenosynovitis. In this study, it was aimed to investigate diagnose of the disease by pathologic and molecular techniquesin hens that naturally infected with MG as well as the usability of immunocytochemical (ICC) method in diagnose of thedisease.Materials, Methods & Results: For this purpose, 98 hens were collected from 10 different coops that serologically positive.After necropsy, routine pathological procedures were performed to samples taken from nose, sinus, larynx, trachea, lungand air sacs. Scraping samples taken from lungs and tracheas were evaluated by ICC. Immunohistochemical (IHC) stainingwas performed to samples taken from nose, sinus, larynx, trachea, lung and air sacs. Indirect immunoperoxidase methodwas applied in the both IHC and ICC staining. Rabbit polyclonal anti MG antibody was used as primer antibody in theIHC and ICC staining. Additionally, culture and PCR techniques were applied to tracheas of all hens for MG. The GPO3and MGSO genes were made for PCR analysis. In the tracheal examinations, 23 cases were positive for PCR, 17 casesICC positive, 16 cases IHC positive and 10 culture samples found positive. All of culture positive cases were also positivefor other three methods. When findings in all organs were evaluated, in 37 cases were detected positive by IHC (38%) and23 cases were positive by ICC (23.5%). In the IHC positive cases, the first order was trachea in 16 cases followed by in 11cases in sinus, in 8 cases in lung, in 6 cases air sac and 4 cases in nose, respectively. In 8 cases, IHC positivity was foundin at least two organs...(AU)

Investigations of pathological immunohistochemical and immunocytochemical findings in natural infection with Mycoplasma gallisepticum in laying hens

Background: Mycoplasmosis is an infectious disease caused by Mycoplasma gallisepticum (MG), usually seen in therespiratory system of chickens, chick and turkeys, that causing great economic loss. The disease is characterized by respiratory system lesions such as sinusitis, tracheitis, airsacculitis, pneumonia and other symptoms such as loss of yield, arthritis,tenosynovitis. In this study, it was aimed to investigate diagnose of the disease by pathologic and molecular techniquesin hens that naturally infected with MG as well as the usability of immunocytochemical (ICC) method in diagnose of thedisease.Materials, Methods & Results: For this purpose, 98 hens were collected from 10 different coops that serologically positive.After necropsy, routine pathological procedures were performed to samples taken from nose, sinus, larynx, trachea, lungand air sacs. Scraping samples taken from lungs and tracheas were evaluated by ICC. Immunohistochemical (IHC) stainingwas performed to samples taken from nose, sinus, larynx, trachea, lung and air sacs. Indirect immunoperoxidase methodwas applied in the both IHC and ICC staining. Rabbit polyclonal anti MG antibody was used as primer antibody in theIHC and ICC staining. Additionally, culture and PCR techniques were applied to tracheas of all hens for MG. The GPO3and MGSO genes were made for PCR analysis. In the tracheal examinations, 23 cases were positive for PCR, 17 casesICC positive, 16 cases IHC positive and 10 culture samples found positive. All of culture positive cases were also positivefor other three methods. When findings in all organs were evaluated, in 37 cases were detected positive by IHC (38%) and23 cases were positive by ICC (23.5%). In the IHC positive cases, the first order was trachea in 16 cases followed by in 11cases in sinus, in 8 cases in lung, in 6 cases air sac and 4 cases in nose, respectively. In 8 cases, IHC positivity was foundin at least two organs...

Mesenchymal stem cells reduce left ventricular mass in rats with doxorubicin-induced cardiomyopathy / Las células madre mesenquimales reducen la masa ventricular izquierda en ratas con miocardiopatía inducida por doxorrubicina

SUMMARY: Doxorubicin is a drug that used by a majority in the treatment of carcinomas. The most obvious known side effect is cardiomyopathy. Many studies have been carried out to eliminate side effects of the doxorubicin, and stem cell studies have been added in recent years. In this study, it was aimed to investigate fetal-derived mesenchymal stem cells (F-MSCs) treatment of doxorubicininduced cardiomyopathy by morphological methods. A total of 24 rats which were divided into three separate groups (Control, sham, treatment), each consisting of 8 male rats were used. In sham and treatment group, Adriamycin was administered in a single dose by tail injection to perform cardiotoxicity. In the treatment group, F-MSCs were intra-peritoneally administrated. Then, rats were euthanized and their hearts were photographed at the level of papillary muscle. and thickness, diameters and surface area levels were measured. Left ventricular mass (LVM) and left ventricular mass index (LVMI) were calculated after measurement. The sham group, LVM and LVMI levels were found to significantly lower (p<0.05) than control and treatment group. In the one hand, LVMI levels of rats in treatment group was statistically similar (p>0.05) to control group. Similarly, LVM levels of control and treatment groups were close to each other while this level of sham group was lower. It has been shown that F-MSC administrations in rats with doxorubicin-induced cardiomyopathy have adverse effect on LVM and LVMI values. In addition, the intra-peritoneal MSC administrations may be an alternative to other injection routes such as intra-venous and intra-cardiac administrations.

RESUMEN: La doxorrubicina es un medicamento usado ampliamente en el tratamiento de carcinomas. El efecto secundario más conocido es la miocardiopatía. Se han llevado a cabo muchos estudios para eliminar los efectos secundarios de la doxorrubicina, y en los últimos años se han agregado estudios con células madre. mediante métodos morfológicos, se intentó investigar el tratamiento de las células madre mesenquimales (F-MSCs) derivadas del feto, de la miocardiopatía inducida por doxorrubicina. Se utilizó un total de 24 ratas que se dividieron en tres grupos (control, simulación, tratamiento), cada uno de las cuales consistía en 8 ratas macho. En el tratamiento simulado y en el grupo tratamiento, se administró doxorrubicina en una dosis única mediante inyección en la cola de la rata para realizar cardiotoxicidad. En el grupo tratamiento, las FMSC se administraron intraperitonealmente. Luego, las ratas fueron sacrificadas y sus corazones fueron fotografiados a nivel de los músculos papilares, y se midieron los espesores, los diámetros y los niveles de área superficial. Después de las mediciones se calcularon la masa ventricular izquierda (MVI) y el índice de masa ventricular izquierda (IMVI). En el grupo simulado, los niveles de MVI y IMVI se encontraron significativamente inferiores (p <0.05) que en los grupos control y tratamiento. Por un lado, los niveles de IMVI de las ratas en el grupo de tratamiento fueron estadísticamente similares (p> 0,05) al grupo de control. De forma similar, los niveles de MVI de los grupos control y tratamiento se aproximaban uno al otro, mientras que este nivel era más bajo en el grupo simulado. Se ha demostrado que la administracion de F-MSC en ratas con miocardiopatía inducida por doxorrubicina tiene un efecto adverso sobre los valores de MVI y IMVI. Además, la administracion de MSC intraperitoneal puede ser una alternativa a otras rutas de inyección tal como las administración intravenosa e intracardíaca.

Comparison of thermographic imaging and other diagnostic techniques in diagnosis of cattle with laminitis

Background: Lameness results in major economic losses on dairy farms. The proportion of lameness in cows are between 4-55%, the origin of 90% lameness are caused by foot diseases. Hoof abnormalities such as laminitis are major contributors to lameness. Aim of the study, compared with thermographic imaging (IRT) and radiographic, magnetic resonans (MR), computer tomography (CT), histopathological analysis on the diagnosis of laminitis.Materials, Methods & Results: Preliminary, the animals (20 Holstein-Fresian) were selected by lameness examination for evaluation of the for any obvious signs of disease. The animals were examined when rising and walking of the free stalls. Walking was very painful and clinical symptoms of general distress become present for laminitic group. The cows were scored on a 1 to 5 scale for their locomotion (1 = normal locomotion; 5 = severely lame). Thermographic examinations were performed the all animals after the routine clinical examination methods. The infrared images were taken from the dorsal view of all hoovesto monitor the temperature of coronary band. All images were scanned using a hand-held portable infrared camera (Wahl, Thermal Imager HSI3000 Series), which was calibrated to ambient temperature and absorptive conditions. Incentives radiological examinations, dorso-palmar and latero-medial shooting was done for using of (Regius Model 110 Konica, Minolta) 70 kV and 30 mAs for each claws. MRI images of all isolated digits were performed at the Department of Radiology, by using Siemens Symphony Erlangen, Germany. Continuous series of transversal, sagital and dorsoplantar scan were obtained from all digit. Computer tomography (CT), isolated digits were performed at the Imaging Department of Radiology, by using Siemens Symphony Erlangen, Germany.[...](AU)

Comparison of thermographic imaging and other diagnostic techniques in diagnosis of cattle with laminitis

Background: Lameness results in major economic losses on dairy farms. The proportion of lameness in cows are between 4-55%, the origin of 90% lameness are caused by foot diseases. Hoof abnormalities such as laminitis are major contributors to lameness. Aim of the study, compared with thermographic imaging (IRT) and radiographic, magnetic resonans (MR), computer tomography (CT), histopathological analysis on the diagnosis of laminitis.Materials, Methods & Results: Preliminary, the animals (20 Holstein-Fresian) were selected by lameness examination for evaluation of the for any obvious signs of disease. The animals were examined when rising and walking of the free stalls. Walking was very painful and clinical symptoms of general distress become present for laminitic group. The cows were scored on a 1 to 5 scale for their locomotion (1 = normal locomotion; 5 = severely lame). Thermographic examinations were performed the all animals after the routine clinical examination methods. The infrared images were taken from the dorsal view of all hoovesto monitor the temperature of coronary band. All images were scanned using a hand-held portable infrared camera (Wahl, Thermal Imager HSI3000 Series), which was calibrated to ambient temperature and absorptive conditions. Incentives radiological examinations, dorso-palmar and latero-medial shooting was done for using of (Regius Model 110 Konica, Minolta) 70 kV and 30 mAs for each claws. MRI images of all isolated digits were performed at the Department of Radiology, by using Siemens Symphony Erlangen, Germany. Continuous series of transversal, sagital and dorsoplantar scan were obtained from all digit. Computer tomography (CT), isolated digits were performed at the Imaging Department of Radiology, by using Siemens Symphony Erlangen, Germany.[...]