The maternal genetic origin and diversity of the extant populations of the Ladakh region in India.

Ladakh lies at a strategic location between the Indus River valley and the Hindu Khush Mountains, which makes the "Land of high passes" one of the major routes of movement. Through the years the region has faced multi-layered cultural movements, genetic assimilation and demographic changes. The initial settlement in the years goes back to the early Neolithic age and still continues despite its harsh, unhospitable and cold climate. Previous studies mostly covered the patrilineal markers of the region and an in-depth study lacked to represent the matrilineal ancestry and possible genetic inflow in the region. Hence, our current study first time generated complete mitogenomes of 108 unrelated individuals from Ladakh belonging to three population groups namely, Changpa (n = 38), Brokpa (n = 32) and Monpa (n = 38). In the in-depth analysis, we found that the mitogenome of the three Ladakhi groups are highly diverse in terms of maternal haplogroup distribution carrying lineages specific to East Asia (M9a), Tibbet (A21) and South Asia (M3, M30, U2). In our analysis we found that Changpa and Monpa probably have shared maternal ancestry compared to Brokpa, which is very distinct and also later suffered possible historical Bottleneck. Bayesian evolutionary and Network analysis indicates more ancient maternal lineage of Changpa and Monpa in terms of M9a haplotypes, but they also share some genetic history with Tibeto-Burman speakers in past. These findings conclusively indicate possible matrilineal genetic inflow in Ladakh from three directions, primarily from East Asia or South East Asia during post-glacial, West Eurasia and also from South Asia.

Medicinal plants traditionally used in the health care practices by the indigenous communities of the Trans-Himalayan region of Ladakh, India.

ETHNOPHARMACOLOGICAL RELEVANCE: Ladakh, "the land of high-rising passes," is a cold arid desert located in the India's northernmost part of the Trans-Himalayan region. Traditional knowledge of medicinal plants in this fragile ecosystem is an important part of the primary healthcare system, particularly in remote areas where modern medical facilities are not fully operational. There is a need to update the traditional information on medicinal plants from time to time to understand any addition of plants or uses to assist in developing new drugs through pharmacological and phytochemical studies. AIM OF THE STUDY: The study was conducted to document the traditional knowledge and current therapeutic practices of the indigenous communities of Ladakh. Besides, the study strives to evaluate previous studies from Ladakh to identify plants that have not been previously reported for medicinal use. MATERIAL AND METHODS: Ethnomedicinal information was collected from 350 local informants through open and semi-structured questionnaires and field surveys in 35 villages of 8 regions of Ladakh. The primary data were analyzed for use reports (UR) and the informant consensus factor (ICF). The reported diseases were classified according to the International Classification of Primary Care-2 (ICPC-2). New reported medicinal plants and unreported medicinal uses in this study were identified and compared to 36 prior studies conducted in Ladakh. RESULTS: In the study, 176 medicinal plants belonging to 45 families and 131 genera were reported to treat 116 diseases of 16 (ICPC-2) groups with a total of 4841 use reports. Based on the highest use reports (UR), the most utilized species were Thymus linearis Benth. (134), followed by Aconitum heterophyllum Wall. (127) The highest informant consensus factor (ICF) was reported for Pregnancy, Childbearing, and Family Planning (ICF = 1) followed by respiratory (ICF = 0.91), and digestive (ICF = 0.90) disorders. Based on the comparative literature review, a total of 518 plant species (502 previously reported plus 16 newly cited) are being used in the Ladakh region. A total of 133 use reports for 16 newly cited plants using 30 diseases were recorded. The highest degree of overlapping of medicinal plants and uses for medicines was recorded in regions with comparable ethnicity, and shorter geographical distances. Based on the IUCN Red List, recorded medicinal plants include 7 critically endangered, 9 endangered, and 13 vulnerable species. CONCLUSION: Ladakh's indigenous populations use a diverse range of medicinal plants to treat a variety of illnesses. The introduction of species and medicinal uses not previously cited in the primary health care system demonstrates that shared knowledge of traditional medicine among Ladakhi is still rich. The medicinal value of preferred medicinal plants has already been validated, but some medicinal plants lack scientific validation. We recommend further scientific studies on Aconitum violaceum Jacquem. ex Stapf,Anaphalis nepalensis var. monocephala (DC.) Hand.-Mazz., Allardia nivea Hook. f. & Thomson ex C.B. Clarke, Atriplex hortensis L., Eriophyton tibeticum (Vatke) Ryding, Iris lactea Pall. and Rheum webbianum Royle.

Disaster-development interface and its impact on emerging vulnerability scenario in Ladakh region of northwestern Himalayas.

The Union Territory of Ladakh, located in the northwestern Himalayan region, is highly vulnerable to natural and anthropogenic hazards like earthquakes, landslides, snow avalanches, flash floods, cloud bursts, and border conflicts. Occurrences of these disasters have significantly influenced the development and vulnerability scenario of Trans-Himalayan Ladakh. Findings reveal that despite suffering losses from natural and human-induced disasters, the region has benefited by grabbing the attention of policymakers at the national level. Consequently, long-term developments were positively impacted, reflecting infrastructural upgradation, improved transportation and communication, profoundly improving the socio-economic well-being of the people. Furthermore, post-disaster developments have managed to showcase the unique physiography and adventurous terrains of Ladakh, promoting tourism as the main economic driver in the region. The exponential growth of tourism and associated sectors have influenced the vulnerability scenario, which was quantified using the multi-criterion-based analytical hierarchical processes (AHP) method, indicating an increase in climate change-related vulnerability, followed by socio-cultural, environmental, and physical vulnerabilities. Specifically, the vulnerabilities with respect to flash floods, landslides, erratic rainfall, haphazard constructions, cultural dilution, water crisis, and changes in land use patterns have been exacerbated across the study area. The study highlights the need for effective management of these emerging vulnerabilities through proper planning to ensure long-term sustainable development goals in this environmentally fragile region.

On the move: spatial ecology and habitat use of red fox in the Trans-Himalayan cold desert.

Red fox (Vulpes vulpes) is the most widespread wild carnivore globally, occupying diverse habitats. The species is known for its adaptability to survive in dynamic anthropogenic landscapes. Despite being one of the most extensively studied carnivores, there is a dearth of information on red fox from the Trans-Himalayan region. We studied the home range sizes of red fox using the different estimation methods: minimum convex polygon (MCP), kernel density estimator (KDE), local convex hull (LoCoH) and Brownian-bridge movement model (BBMM). We analysed the daily movement and assessed the habitat selection with respect to topographic factors (ruggedness, elevation and slope), environmental factor (distance to water) and anthropogenic factors (distance to road and human settlements). We captured and GPS-collared six red fox individuals (three males and three females) from Chiktan and one female from Hemis National Park, Ladakh, India. The collars were programmed to record GPS fixes every 15-min. The average BBMM home range estimate (95% contour) was 22.40 ± 12.12 SD km2 (range 3.81-32.93 km2) and the average core area (50% contour) was 1.87 ± 0.86 SD km2 (range 0.55-2.69 km2). The estimated average daily movement of red fox was 17.76 ± 8.45 SD km/d (range 10.91-34.22 km/d). Red fox significantly selected lower elevations with less rugged terrain and were positively associated with water. This is the first study in the Trans-Himalayan landscape which aims to understand the daily movement of red fox at a fine temporal scale. Studying the movement and home range sizes helps understand the daily energetics and nutritional requirements of red fox. Movement information of a species is important for the prioritisation of areas for conservation and can aid in understanding ecosystem functioning and landscape management.

Correlation of Helicobacter pylori virulence genotype & severity of mucosal inflammation in gastric biopsies from two geographically diverse regions in India.

Background: H. pylori-associated gastritis in patients from the high-altitude area of Ladakh showed severe gastritis, mucosal nodularity, atrophy, and cancer in comparison to those from North India. This study served to analyze if differences in the H. pylori virulence genotypes decide the extent of gastric mucosal inflammation. Methods: Fifty gastric biopsies each from patients with H. pylori-associated gastritis from Ladakh and a tertiary care center in North India were included. The presence of H. pylori strain was confirmed with Warthin starry stain and polymerase chain amplification of the H. pylori-specific 16S rRNA. The cagA, vacA s1, s2, and m1, m2 alleles, and dupA virulence genotypes were studied in all archival samples, followed by their histological correlations. Results: cagA (P 0.009) and vacAs1 m1 (P 0.009) genes were distinctly more in H. pylori strains colonizing the biopsies of North Indian patients. In contrast, the cagA -ve vacAs2 m2 strains were significantly more in H. pylori strain colonizing the biopsies from Ladakhi patients. dupA genotype was almost similarly present in strains from both regions. Among these, only cagA and dupA virulence genes were associated with severe mucosal neutrophilic activity and deep infiltration of H. pylori strains in North Indian patients. Conclusions: Differences in virulence genotypes of H. pylori in gastric biopsies from North Indian and Ladakhi patients were found not significant in deciding the severity of H. pylori-associated gastritis.

Ascertaining glacier dynamics and geodetic mass changes in the Pangong Region of Trans-Himalayan Ladakh using remote sensing data.

Glaciers in the Himalayan arc are receding rapidly in the eastern and western parts as compared to other regions. Contrararily, the glaciers in the Trans-Himalayan region of Ladakh are comparatively stable. The differential retreat could be due to various climatic, topographic, and geologic influences. The use of multi-source remotely sensed imagery from open-source platforms and the GlabTop model has been discussed in this paper. This paper draws insights from a recently published paper which details the recession of 87 glaciers in the Trans Himalayan region of Ladakh using remote sensing data [1]. The use of remote sensing data from USGS and Planet Labs for assessing glacier area changes, frontal retreat, debris cover, topographic characteristics, and comparison with existing inventories has been discussed in this study. The geodetic mass changes have been assessed using SRTM and TanDEM-X of 2000 and 2012 respectively. The use of remotely sensed data discussed in this article will help glaciologists to better characterize and understand the glacier recession in the region. The GlabTop model has been used to simulate proglacial lake expansion to understand glacier-bed overdeepenings of four glaciers in the region. The GlabTop simulations will help disaster managers to better quantify the vulnerability and risk of downstream population and infrastructure to Glacial Lake Outburst Floods (GLOFs).

Anthropogenic climate change drives melting of glaciers in the Himalaya.

The Himalayan glaciers provide water to a large population in south Asia for a variety of purposes and ecosystem services. As a result, regional monitoring of glacier melting and identification of the drivers are important for understanding and predicting future cryospheric melting trends. Using multi-date satellite images from 2000 to 2020, we investigated the shrinkage, snout retreat, thickness changes, mass loss and velocity changes of 77 glaciers in the Drass basin, western Himalaya, India. During this period, the total glacier cover has shrunk by 5.31 ± 0.33 km2. The snout retreat ranged from 30 to 430 m (mean 155 ± 9.58 m). Debris cover had a significant impact on glacier melting, with clean glaciers losing ~ 5% more than debris-covered glaciers (~ 2%). The average thickness change and mass loss of glacier have been - 1.27 ± 0.37 and - 1.08 ± 0.31 m w.e.a-1, respectively. Because of the continuous melting and the consequent mass loss, average glacier velocity has reduced from 21.35 ± 3.3 m a-1 in 2000 to 16.68 ± 1.9 m a-1 by 2020. During the observation period, the concentration of greenhouse gases (GHGs), black carbon (BC) and other pollutants from vehicular traffic near the glaciers increased significantly. Increasing temperatures, caused by a significant increase in GHGs, black carbon and other pollutants in the atmosphere, are driving glacier melting in the study area. If the current trend continues in the future, the Himalayan glaciers may disappear entirely, having a significant impact on regional water supplies, hydrological processes, ecosystem services and transboundary water sharing.

Occurrence of GI parasites in ruminants of Kashmir and Ladakh.

The study was conducted in Kashmir and Ladakh Divisions of the erstwhile state of Jammu and Kashmir to work out the occurrence of gastrointestinal parasites in ruminants. The qualitative faecal examination of 476 samples revealed an overall prevalence of GI parasites to the tune of 80.04% comprising of 80.61% in cattle, 96.70% in sheep and 66.27% in goats. In Kashmir overall prevalence of GI parasites was 88.33% comprising of 80.61% in cattle, 98.41% in sheep and 100% in goats. Highest prevalence was observed for strongyles (84.00%) followed by Eimeria spp. (31.67%), Moniezia spp. (20.00%), Ascaris spp. (5.33%) and Strongyloides spp. (1.67%). Mixed infection was observed in 37.67% samples. In cattle, the parasites observed were strongyles (68.37%) and Eimeria spp. (13.27%) with mixed infection in 1.02% samples. In sheep highest prevalence was observed for strongylid eggs (97.88%) followed by Eimeria spp. (43.38%), Moniezia spp. (31.74%), Ascaris spp. (8.47%) and Strongyloides spp. (2.64%) with mixed infection in 59.26% samples. Amongst goats, only strongylid eggs (100%) were observed. In Ladakh region overall prevalence of GI parasites was 65.91% with 82.61% in sheep and 63.40% in pashmina goats. Highest prevalence was observed for Eimeria spp. (63.64%) followed by strongyles (9.09%) Moniezia spp. (5.11%) and Thysanosoma spp. (1.14%). Mixed infection was observed in 13.07% samples. In sheep, highest prevalence was observed for Eimeria spp. (73.91%) followed by strongyles (17.39%) and Thysanosoma spp. (8.70%) with mixed infection in 17.39% samples. In pashmina goats, the prevalence of Eimeria spp. (62.09%) was observed highest followed by strongylid eggs (7.84%) and Moniezia spp. (5.88%) with mixed infection in 12.42% samples.

Case Series of Neurotrauma Managed by General Surgeon at Ladakh - The Highest Plateau State of India.

Trauma including neurotrauma is one of the major killers in the world. The health infrastructure in developing countries has a huge disparity, with super specialist care confined to big cities only. Adding to this problem is factors like poverty, poor road condition and no organised emergency evacuation system. The utopian scenario where specialist and infrastructure are made available in every village is a distant dream. So at present, the most feasible and cost-effective way to prevent death due to neurotrauma in rural and remote setting is putting the general surgeon working in remote area at the forefront. Our study is an effort in this direction and is reporting operative management of patients with neurotrauma in remote high-altitude Ladakh. So today, the need of hour is continuing skill enhancement training for capacity building of rural surgeon, where they are trained to do burr hole and craniotomy for reducing mortality and morbidity from neurotrauma. Supplementary Information: The online version contains supplementary material available at 10.1007/s12262-021-03002-x.

Plant Resources Utilization among Different Ethnic Groups of Ladakh in Trans-Himalayan Region.

The nomadic pastoral indigenous communities of the Ladakhi people share roots with Tibetan culture in terms of food, clothing, religion, festivals, and habits, and rely widely on plant resources for survival and livelihood. This survey was conducted during 2019-2021 to document the indigenous knowledge about plant resources of the Balti, Beda, and Brokpa communities of the Ladakh region, trans-Himalayas. Open- and close-ended semi-structured interviews (N = 184) and group discussions (N = 17) were used to collect the data. Quantitative data was further analyzed using various statistical tools. A total of 105 plant species belonging to 82 genera and 39 families were used as medicine, fuel wood, fragrance, oil, food, flavor, fodder, decoration, and dye. Among these, medicinal use was most prevalent, with 70% of use reports, followed by fodder and fuel wood. Leaves (27%) were the most preferred plant part used, followed by roots and flowers. The principal component analysis revealed five clusters of ethnobotanical usage, i.e., food, medicine, fuel wood, fodder, and fragrance, oil, dye, and flavor. The maximum number of plant species used was reported by the Brokpa, while the Beda reported the minimum number of plant species uses. Delphinium brunonianum, Waldheimia tomentosa, and Juniperus indica played a significant role in the cultural and religious ritual aspects, whereas Allium przewalskianum, Waldheimia tomentosa, Juniperus indica, and Hippophae rhamnoides were commonly used as a livelihood source among Ladakhi communities. The local people collected most plants (65%) for self-consumption, while the rest (35%) were sold in markets as a source of income. The sustainable utilization and management of plant resources by local people is a strategy to boost livelihoods and food security and alleviate poverty.

Effect of shading and high temperature amplitude in greenhouse on growth, photosynthesis, yield and phenolic contents of tomato (Lycopersicum esculentum Mill.).

Low temperature is the major environmental factor that limits the optimal field production of tomato in the high altitude mountain regions. Studies were conducted to determine the feasibility of growing tomato, a temperature sensitive crop, in a naturally ventilated passive solar greenhouse with high temperature amplitude (24.7 ± 3.0 °C). The study also aimed to determine the application of shade net combined with low-cost greenhouse technology. Despite the temperature fluctuation from 6.6 ± 2.1 °C at night to 39.1 ± 4.7 °C day temperature, flowering and fruiting were observed under the greenhouse conditions. The marketable yield inside the greenhouse was 1.8-times higher compared to open-field. Shading significantly affected the photosynthesis and results in increased sub-stomatal CO2 concentration. Shading resulted in delayed flowering and 48% reduction in marketable yield. Total phenolic contents (TPC) of tomato grown under open-field and greenhouse conditions were similar. However, greenhouse conditions resulted in a 35% decrease in total flavonoid contents (TFC) of tomato fruit. Shading reduced the TPC and TFC by 29 and 16%, respectively under greenhouse conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-021-01032-z.

Decoding the Karakoram Anomaly.

The 'Karakoram Anomaly' is termed as the stability or anomalous growth of glaciers in the central Karakoram, in contrast to the retreat of glaciers in other nearby mountainous ranges of Himalayas and other mountainous ranges of the world. It remains an intriguing scientific question to the researchers. An attempt is made to provide mechanisms leading to such a process and thus 'affirming' it. In view of this, meteorological and cryospheric processes, viz., glacial-atmosphere coupled interactions in tandem with temperature-moisture interactions and radiative balance- on glaciated regions are simultaneously argued over the Karakoram and the adjacent Ladakh. Ladakh is deliberately chosen to compare the weaknesses, lacuna and gaps in the observations/reanalyzes- so that similar forcings are investigated over both regions. It is important to mention that both regions are data sparse. Findings show that geographical and elevation positioning of the Karakoram makes its environmental conditions conducive for glacier stability and/or growth which otherwise is not the case in the Ladakh region. Indian winter monsoon, western disturbances (WDs) embedded within upper level subtropical westerly jet moving eastwards, provides higher moisture incursion which in association with lowered lifting condensation level dumps higher moisture/mass over Karakoram than Ladakh. In addition, role of 2 m surface (T2m) and skin temperature (Ts) is one of the leading driving mechanisms. Difference (T2m-Ts) illustrates inversion which provides stable atmosphere leading to dump all the available moisture/mass over Karakoram, which is contrary over Ladakh.

First report of the poplar leaf miner, Phyllonorycter populifoliella (Treitschke) (Lepidoptera: Gracillariidae) from India.

Here we report about the discovery of the poplar leaf miner, Phyllonorycter populifoliella (Treitschke) in India. The mines of this micromoth were found in noticeable density on the leaves of poplar, Populus sp. (Salicaceae) in the northern mountainous region Ladakh in 2017-2018. We provide short morphological diagnosis, describe bionomics and analyze molecular data of Ph. populifoliella from India comparing sequences with those from other countries in Eurasia where the species is known as native. We also illustrate male and female genitalia, an adult of the moth, the leaf mines and the infestation plot in Ladakh, and discuss the occurrence of the species in the country.

Distribution and relevance of aufeis (icing) in the Upper Indus Basin.

In the semi-arid high mountains of the Upper Indus Basin (UIB), meltwater supply from the cryosphere is vital for irrigated agriculture and hydropower generation. An overlooked cryosphere component that is critical for this is aufeis, which appears as a sheet-like formation of ice layers, created by successive and laminated freezing of flowing water. This study aims to redress the lack of knowledge about this phenomenon by creating an inventory of aufeis fields for the UIB and analysing their spatial distribution, including the role of topographical parameters such as altitude, slope, and aspect. The study is based on a time-series analysis using Landsat imagery from 2010 to 2020, supported and validated by several field campaigns carried out between 2014 and 2020. In total, more than 3700 aufeis fields were detected covering an area of about 298 ± 38 km2. The spatial distribution of their occurrence indicates a distinct elevation range between 4000 and 5500 m a.s.l. and is marked by a pronounced longitudinal increase to the east. In contrast to the western part of the UIB (Gilgit-Baltistan), where only some few and small aufeis fields can be detected, 65% of the aufeis covered areas (195 ± 23 km2) exist on the Tibetan Plateau. Our database fills an important research gap and will help in further cryosphere studies in the UIB and beyond.

Fresh and dry fruit production in Himalayan Kashmir, Sub-Himalayan Jammu and Trans-Himalayan Ladakh, India.

Jammu & Kashmir and Ladakh are two union territories (UT's) famous for their beauty and cool climate. Both the UT's are familiar with their horticulture production at the national and international level. Jammu & Kashmir offers enough scope for cultivation of all types of horticulture crops covering various sub-tropical fruits like mango, guava, citrus, litchi, temperate fruits like apple, pear, peach, plum, apricot, almonds, cherry, and apricot. Horticulture has emerged as a fast-growing sector in Jammu & Kashmir and to some extent in Ladakh, offers a wide range of opportunities to farmers for crop diversification. Horticulture has proved its credibility in improving income through increased productivity, creating employment, and strengthening exports. Jammu & Kashmir comprises 20 districts and Ladakh two districts, and both having comparative advantages in some specific types of fruit cultivation due to variations in soil fertility, soil matter, topography, and variable to harsh weather. In this survey, we analyze the district wise contribution and improvement of horticulture production of fresh fruits and an increase in their growing area. The survey-based on secondary data sources collected from the Directorate of Horticulture of Jammu & Kashmir and Ladakh. The data is analytical and empirical, directly contributing to the horticulture production of India. The area and production of apple are maximum in district Baramulla, a temperate region of J&K. While, maximum area as well as the production of apricot is in district Leh, a high altitude and cold arid region of Ladakh.

Recession of Gya Glacier and the 2014 glacial lake outburst flood in the Trans-Himalayan region of Ladakh, India.

This study assessed spatiotemporal changes at Gya Glacier, the associated development of a proglacial lake, and reconstructed the 2014 outburst flood that struck Gya Village in the Trans-Himalayan region of Ladakh, India. This study analyzed and for the first time modeled a Glacial Lake Outburst Flood (GLOF) event in the Trans-Himalayan region of Ladakh. Glacier and glacial lakes changes were quantified using remote sensing data supplemented with field observations. Glacier ice-thickness and glacier-bed overdeepenings were modeled using a shear-stress based model, GlabTop (Glacier-bed Topography). The reconstruction of the 2014 GLOF and the potential hazard assessment of Gya Lake were carried out using the hydrodynamic model HEC-RAS; results were validated against ground-collected data. Temporal evaluation of satellite data revealed a 45.6% loss in the total glacier area between 1969 and 2019. The earliest snow-free image available for the region shows that a proglacial lake existed as early as 1969 with an area of 3.06 ha. The lake has expanded to ~11 ha in 2019. Results from the GlabTop model suggest that the lake could grow further up to 12 ha in the future. Field-based geomorphic indicators suggest that the 2014 GLOF event resulted from a piping failure of the frontal moraine destroying numerous agricultural fields, some buildings, downstream infrastructure, and eroded natural channel embankments. The reconstruction of the event revealed that 25% of the lake waters drained out with a peak discharge of 470 m3s-1, inundating an area of ~4 km2 around Gya Village. However, a complete breaching of the terminal moraine could result in an event that would be 5.5 times larger than the 2014 GLOF. Therefore, this study could be useful not only in planning disaster-resilient infrastructure around proglacial lake environments in the cold-arid Ladakh but also in framing mitigation plans to reduce risk for vulnerable downstream communities.

Occurrence of granulovirus infecting Cydia pomonella in high altitude cold arid region of India.

Codling moth (Cydia pomonella, Lepidoptera: Tortricidae) is a quarantine pest of apple in Ladakh, India. We report Cydia pomonella granulovirus from infected larvae of codling moth for the first time in India. The two CpGV isolates were identified as (CpGV SKUAST-1 and CpGV SKUAST-2) and published in Genbank under accession number, MK801791 and MK801792, respectively. The mortality of CpGV was evaluated against 3rd instar larvae of codling moth at various concentrations viz., 102, 104, 106, 108, 1010, 1012 and 1014 OBS/ml. The median lethal concentrations (LC50 and LC90) were observed at 7.08 and 28.56 OBS/ml, respectively. In field, the infection rate by CpGV was 5.95 to 15.65%. Based on typical infection symptoms on the larvae, morphological features under the microscope and sequence results of the amplified product confirmed the first occurrence of CpGV from India. Thus, CpGV will form an important non-chemical strategy for managing this pest.

First report of Cryptosporidium sp. infection in sheep population of Ladakh, India.

Ladakh is the high altitude (11,184 ft above sea level) cold desert region in the state of Jammu & Kashmir (India), where winter temperature goes below - 35 °C. A total of 37 faecal samples were collected from local Changthangi and Russian merino sheep of the Leh region of Ladakh, having the history of diarrhoea and emaciation, followed by death. Of the 37 samples collected, 20 (54.05%) showed presence of Cryptosporidium sp. infection on faecal smear examination after Ziehl-Neelsen staining. The number of samples positive for Cryptosporidium sp. infection on faecal smear examination was 7 and 13 for Russian Merino and local Changthangi sheep, respectively. The occurrence of Cryptosporidium sp. infection was similar in both sexes, 11/20 (55%) in females and 9/20 (45%) for males. The Age-wise occurrence of Cryptosporidium sp. infection in different age groups was found to be: 50% in less than 2 year age group and 50% in more than 2 year age group. To the best of our knowledge, this is the first report of Cryptosporidium sp. infection in adult sheep from India. The survival of the parasite at such freezing temperature gives the insight about its cold-resistant nature.

Anthropometric evaluation of school-going native highlanders (4-19 years of age) from the Leh-Ladakh region in India.

BACKGROUND: The native population of the Ladakh region faces the unique challenges of a high-altitude environment with distinct physiological adaptations in comparison with lowlanders. However, no comprehensive data on standard anthropometric parameters for the school-going children in this populace is available. OBJECTIVES: We aimed to study the various anthropometric parameters in the school-going native highlander population and computed measures of central tendency. The nutritional status of the community was also be determined by comparing with World Health Organization (WHO) scores for height for age (HFA), weight for age (WFA) and body mass index (BMI) for age. DESIGN: A cross-sectional, descriptive study was devised to assess the anthropometric parameters. We measured height, weight, mid-upper circumference (MUAC), triceps skinfold (TSF) thickness, sub-scapular skinfold (SSF) thickness, waist, hip and abdominal circumference. Statistical analysis was conducted to determine the mean [±2 standard deviation (SD)], median, range, minimum and maximum. The z-scores for HFA, WFA and BMI for age was computed using WHO reference data. SUBJECTS: A total of 346 school-going native highlander children (4-19 years of age) were studied. RESULTS: Among the study population, the mean height was 141.17 ± 39.08 cm, the mean weight was 38.27 ± 25.40 kg. The gender difference in height, MUAC, sub-scapular skinfold (SSF) thickness, TSF thickness and the abdominal circumference was found to be statistically significant. Of the subjects 23.46% were stunted (i.e. HFA below -2 SD of the WHO standard) and 7.01% were underweight (WFA below -2 SD of the WHO standard). CONCLUSION: The nutritional status of the Ladakhi population was assessed by comparison with the WHO reference data. Nomograms for anthropometric data in school-going children (4-19 years of age) of Ladakh were created. These can be used for further studies and planning targeted intervention strategies on this geographically isolated and evolutionary distinct highland population.

Erythropoietin and ferritin response in native highlanders aged 4-19 years from the Leh-Ladakh region of India.

The pivotal role of erythropoietin (EPO) in hypoxic adaptation has led to various studies assessing the EPO and ferritin response in native highlanders from Andes and Tibet. We assessed the relationship between EPO, haemoglobin and ferritin in 335 native highlanders (172 boys and 163 girls, aged 4 to 19 years) from Leh-Ladakh, India, who had no history of travel to lowland areas. Complete blood counts, serum EPO and ferritin levels were measured. We stratified study subjects based on age, gender, pubertal status and analysed the EPO and ferritin levels between the stratified groups respectively. The mean EPO level in boys was lower than girls. The mean ferritin level in boys was significantly higher (P = 0·013) than in girls. There was no significant variation in the EPO and ferritin levels amongst the various age groups in our study. Near normal EPO levels since childhood with a negative correlation with haemoglobin is suggestive of a robust adaptive mechanism to high altitude from the early years of life. Low ferritin levels are indicative of decreased iron stores in these native highlanders.