ABSTRACT
Angiomatoid fibrous histiocytoma (AFH) is a rarely metastasizing neoplasm that typically occurs in the deep dermis and subcutis of the extremities of young patients, characterized by a t(2;22) translocation involving EWSR1 and CREB1. Because of its distinctive histologic features, the diagnosis of AFH is generally straightforward, although the immunohistochemistry (IHC) findings are relatively nonspecific. We recently encountered a case of primary cranial AFH that showed strong MUC4 IHC expression, which has not yet been reported previously. Prompted by this surprising finding, we investigated MUC4 expression in a series of AFH to evaluate this potential diagnostic pitfall. The expression of ALK by IHC, recently discovered in AFH, was also assessed in this study. We also analyzed EWSR1 rearrangement by fluorescence in situ hybridization using a dual color break-apart probe to confirm the diagnosis. The results showed MUC4 expression in 22.2% of AFH cases (4/18 cases), demonstrating a variable intensity of cytoplasmic staining. Most notably, one of the positive cases showed strong and diffuse expression. ALK IHC expression was observed in 17 of 18 cases (94.4%), usually in a diffuse and strong cytoplasmic pattern. EWSR1 rearrangement was demonstrated by fluorescence in situ hybridization in 81.2% of cases (13 of 16), including all the MUC4-positive cases. Our results indicate that although the significance of MUC4 expression in AFH is unknown, it is important to be aware that a subset of AFH can express the protein by IHC, expanding a variety of MUC4-positive mesenchymal tumors.
Subject(s)
Histiocytoma, Benign Fibrous/metabolism , Mucin-4/metabolism , Adolescent , Adult , Aged , Anaplastic Lymphoma Kinase/metabolism , Child , Child, Preschool , Female , Gene Rearrangement , Histiocytoma, Benign Fibrous/genetics , Humans , Immunohistochemistry , In Situ Hybridization, Fluorescence , Male , Middle Aged , RNA-Binding Protein EWS/geneticsABSTRACT
Two cases of an unusual cutaneous lesion characterized by a dermal nodule with apocrine gland cysts surrounded by a hemosiderotic dermatofibroma-like proliferating stroma are reported. The first case was a 52-year-old female who presented with a nodule on the back of several years of evolution and with the clinical diagnosis of lipoma; the second case was a 41-year-old male who presented with a forehead nodule of several years of evolution with a clinical diagnosis of epidermal cyst. Histologically, an ill-delimited non-encapsulated nodule composed of cystic spaces and solid areas was found. The cystic spaces consisted of ductal structures and a bilayered epithelial covering with apocrine differentiation; the surrounding area showed a stroma composed of a mixture of both fibrocytes and macrophages and abundant hemosiderin granules; foci of recent hemorrhages and a more dense fibrous stroma at the periphery with typical storiform areas were also visible. Both cases have shown a benign course without local recurrence in follow up until this report date.
Subject(s)
Apocrine Glands/pathology , Epidermal Cyst/pathology , Hemosiderin/metabolism , Histiocytoma, Benign Fibrous/pathology , Skin Neoplasms/pathology , Adult , Apocrine Glands/metabolism , Apocrine Glands/surgery , Epidermal Cyst/metabolism , Epidermal Cyst/surgery , Female , Histiocytoma, Benign Fibrous/metabolism , Histiocytoma, Benign Fibrous/surgery , Humans , Male , Middle Aged , Skin Neoplasms/metabolism , Skin Neoplasms/surgery , Stromal Cells/pathologySubject(s)
Antigens, Helminth/therapeutic use , Glycosaminoglycans/biosynthesis , Histiocytoma, Benign Fibrous/therapy , Immunization/methods , Schistosoma mansoni/immunology , Skin Neoplasms/therapy , Animals , Histiocytoma, Benign Fibrous/metabolism , Histiocytoma, Benign Fibrous/pathology , Mice , Neoplasm Transplantation , Skin Neoplasms/metabolism , Skin Neoplasms/pathologyABSTRACT
Os objetivos do presente trabalho foram estdar os efeitos da deficiência aguda de tiamina no crescimento e na resposta quimiterápica de um fibro-histiocitoma maligno no rato. Para tanto, ratos foram inoculados por via subcutânea com suspensäo de células tumorais e divididos em quadro grupos, de 10 animais cada: DCT - alimentados com dieta sem tiamina; TGT - alimentados com dieta controle; e TVC - alimentados com dieta controle e tratados com SVC. Os animais tratados com SVC (DVC e TVC) apresentaram diminuiçäo de volume tumoral quando comparados com os näo tratados (DCT e TCT). Entretanto, näo houve alteraçäo no volume tumoral de animais carentes em tiamina tratados (DVC) em relaçäo àqueles tratados e com dieta controle (DCT). Estes dados indicam que a deficiência aguda de tiamina per se näo altera o cresciento do FHM como também näo modifica a resposta de tumor frente à quimioterapia com SVC.
Subject(s)
Animals , Male , Rats , Histiocytoma, Benign Fibrous/metabolism , Thiamine Deficiency/metabolism , Body Weight , Histiocytoma, Benign Fibrous/drug therapy , Injections, Intraperitoneal , Rats, Wistar , VincristineABSTRACT
The authors report a case of fibrohistiocytoma of the limbus and discuss the clinical, histopathological and immunohistochemical findings concerning this type of lesion, with a comparison of their findings with those reported in the literature.
Subject(s)
Corneal Diseases/pathology , Eye Neoplasms/pathology , Histiocytoma, Benign Fibrous/pathology , Aged , Biomarkers, Tumor/metabolism , Corneal Diseases/metabolism , Eye Neoplasms/metabolism , Female , Histiocytoma, Benign Fibrous/metabolism , Humans , ImmunohistochemistryABSTRACT
Cytosols from 12 nasopharyngeal angiofibroma tissues were analyzed for the presence of estrogen (ER), progesterone (PR), androgen (AR), and glucocorticoid (GR) receptors, by using a dextran charcoal assay. Progesterone receptors (58%) clearly predominate over ER and AR (25%). Thus, nasoangiofibromas can be classified as ER-PR+ tumors. Glucocorticoid receptors were found in 84% of the tumors. Steroid receptor levels, although slightly higher than those determined in three specimens of normal turbinate, were quantitatively low. Positive PR and AR tumors appeared to correlate with high density of fibroblasts and endothelial cells.
Subject(s)
Histiocytoma, Benign Fibrous/metabolism , Nasopharyngeal Neoplasms/metabolism , Receptors, Androgen/analysis , Receptors, Estrogen/analysis , Receptors, Glucocorticoid/analysis , Receptors, Progesterone/analysis , Adolescent , Adult , Humans , MaleABSTRACT
The immunoperoxidase avidin-biotin-peroxidase complex method was used to investigate the presence of histiocyte markers such as lysozyme, alpha-1-antitrypsin (A1AT) and alpha-1-anti-chymotrypsin (A1ACT) and of vimentin, a specific marker for mesenchymal differentiation, in a spontaneous and transplantable rat tumor of supposed fibroblastic-histiocytic origin. Positive staining was obtained for lysozyme and vimentin but A1AT and A1ACT were not demonstrable in any of the tumor sections. These results provide evidence for the fibro-histiocytic nature of the tumor studied and suggest its classification as a malignant fibrous histiocytoma (MFH).
Subject(s)
Histiocytoma, Benign Fibrous/metabolism , Muramidase/metabolism , Vimentin/metabolism , Animals , Immunoenzyme Techniques , Neoplasms, Experimental/metabolism , Rats , Rats, Inbred Strains , alpha 1-Antichymotrypsin/metabolism , alpha 1-Antitrypsin/metabolismABSTRACT
The inmmunoperoxidase avidin-biotin-peroxidase complex method was used to investigate the presence of histiocyte markers such as lysozyme, alpha-1-antitrypsin (A1AT) and alpha-1-anti-chymotrypsin (A1ACT) and of vimetin, a specific marker for mesenchcymal differentiation, in a sspontaneous and transplantable rat tumor of supposed fibroblastic-histiocytic origin. Positive staining was obtained for lysozyme and vimetin but A1AT and A1ACT were not demonstrable in any of the tumor sections. These results provide evidence for the fibro-histiocytic nature of the tumor studied and suggest its classification as a malignant fibrous histiocytoma (MFH)
Subject(s)
Rats , Animals , Histiocytoma, Benign Fibrous/metabolism , Muramidase/metabolism , Vimentin/metabolismABSTRACT
Se presentan 4 casos de histiocitomas fibrosos malignos primitivos del tórax, diagnosticados y tratados entre 1981 y 1987. Se trata de tumores cuya localización torácica es poco habitual, con pronóstico desfavorable por su elevada tendencia a la recediva local y a las metástasis. El diagnóstico histológico generalmente es incorrecto si se efectúan métodos clásicos de examen y si se practican sobre muestras biópsicas de reducción tamaño. Se resalta la importancia de utilizar procedimiento inmunohistoquímicos para tipificar la lesión, ya que en esta serie los 4 diagnósticos iniciales fueron modificados luego de efectuar las técnicas mendionadas. El tratamiento electivo en casos de tumores torácicos circunscriptos es la exéresis complementada con radioterapia. La quimioterapia se indica para recidivas o metástasis
Subject(s)
Middle Aged , Humans , Male , Female , Histiocytoma, Benign Fibrous/metabolism , Mediastinal Neoplasms , Neoplasm Recurrence, Local , Thoracic Neoplasms , Histiocytoma, Benign Fibrous/pathology , Immunologic Tests , Thoracic Neoplasms/diagnosisABSTRACT
Se presentan 4 casos de histiocitomas fibrosos malignos primitivos del tórax, diagnosticados y tratados entre 1981 y 1987. Se trata de tumores cuya localización torácica es poco habitual, con pronóstico desfavorable por su elevada tendencia a la recediva local y a las metástasis. El diagnóstico histológico generalmente es incorrecto si se efectúan métodos clásicos de examen y si se practican sobre muestras biópsicas de reducción tamaño. Se resalta la importancia de utilizar procedimiento inmunohistoquímicos para tipificar la lesión, ya que en esta serie los 4 diagnósticos iniciales fueron modificados luego de efectuar las técnicas mendionadas. El tratamiento electivo en casos de tumores torácicos circunscriptos es la exéresis complementada con radioterapia. La quimioterapia se indica para recidivas o metástasis (AU)