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1.
J Nanobiotechnology ; 22(1): 468, 2024 Aug 06.
Article in English | MEDLINE | ID: mdl-39103846

ABSTRACT

Ulcerative colitis (UC) is a challenging inflammatory gastrointestinal disorder, whose therapies encounter limitations in overcoming insufficient colonic retention and rapid systemic clearance. In this study, we report an innovative polymeric prodrug nanoformulation for targeted UC treatment through sustained 5-aminosalicylic acid (5-ASA) delivery. Amphiphilic polymer-based 13.5 nm micelles were engineered to incorporate azo-linked 5-ASA prodrug motifs, enabling cleavage via colonic azoreductases. In vitro, micelles exhibited excellent stability under gastric/intestinal conditions while demonstrating controlled 5-ASA release over 24 h in colonic fluids. Orally administered micelles revealed prolonged 24-h retention and a high accumulation within inflamed murine colonic tissue. At an approximately 60% dose reduction from those most advanced recent studies, the platform halted DSS colitis progression and outperformed standard 5-ASA therapy through a 77-97% suppression of inflammatory markers. Histological analysis confirmed intact colon morphology and restored barrier protein expression. This integrated prodrug nanoformulation addresses limitations in colon-targeted UC therapy through localized bioactivation and tailored pharmacokinetics, suggesting the potential of nanotechnology-guided precision delivery to transform disease management.


Subject(s)
Colitis , Colon , Delayed-Action Preparations , Mesalamine , Micelles , Nitroreductases , Polymers , Prodrugs , Animals , Prodrugs/chemistry , Prodrugs/pharmacokinetics , Mesalamine/chemistry , Mesalamine/pharmacokinetics , Nitroreductases/metabolism , Mice , Colon/metabolism , Colon/pathology , Polymers/chemistry , Colitis/drug therapy , Colitis/metabolism , Delayed-Action Preparations/chemistry , NADH, NADPH Oxidoreductases/metabolism , Mice, Inbred C57BL , Colitis, Ulcerative/drug therapy , Colitis, Ulcerative/metabolism , Male
2.
Nat Commun ; 15(1): 6785, 2024 Aug 08.
Article in English | MEDLINE | ID: mdl-39117639

ABSTRACT

Lipopeptides can self-assemble into diverse nanostructures which can be programmed to incorporate peptide sequences to achieve a remarkable range of bioactivities. Here, the influence of peptide sequence and chirality on micelle structure and interactions is investigated in a series of lipopeptides bearing two lysine or D-lysine residues and tyrosine or tryptophan residues, attached to a hexadecyl lipid chain. All molecules self-assemble into micelles above a critical micelle concentration (CMC). Small-angle x-ray scattering (SAXS) is used to probe micelle shape and structure from the form factor and to probe inter-micellar interactions via analysis of structure factor. The CMC is obtained consistently from surface tension and electrical conductivity measurements. We introduce a method to obtain the zeta potential from the SAXS structure factor which is in good agreement with directly measured values. Atomistic molecular dynamics simulations provide insights into molecular packing and conformation within the lipopeptide micelles which constitute model self-assembling colloidal systems and biomaterials.


Subject(s)
Colloids , Lipopeptides , Lysine , Micelles , Molecular Dynamics Simulation , Scattering, Small Angle , Surface-Active Agents , X-Ray Diffraction , Lipopeptides/chemistry , Surface-Active Agents/chemistry , Colloids/chemistry , Lysine/chemistry , Amino Acid Sequence , Surface Tension
3.
Int J Nanomedicine ; 19: 7871-7893, 2024.
Article in English | MEDLINE | ID: mdl-39114180

ABSTRACT

Purpose: Ovarian cancer has the highest mortality rate and lowest survival rate among female reproductive system malignancies. There are treatment options of surgery and chemotherapy, but both are limited. In this study, we developed and evaluated micelles composed of D-α-tocopheryl polyethylene-glycol (PEG) 1000 succinate (TPGS) and Soluplus® (SOL) loaded with olaparib (OLA), a poly(ADP-ribose)polymerase (PARP) inhibitor, and rapamycin (RAPA), a mammalian target of rapamycin (mTOR) inhibitor in ovarian cancer. Methods: We prepared micelles containing different molar ratios of OLA and RAPA embedded in different weight ratios of TPGS and SOL (OLA/RAPA-TPGS/SOL) were prepared and physicochemical characterized. Furthermore, we performed in vitro cytotoxicity experiments of OLA, RAPA, and OLA/RAPA-TPGS/SOL. In vivo toxicity and antitumor efficacy assays were also performed to assess the efficacy of the mixed micellar system. Results: OLA/RAPA-TPGS/SOL containing a 4:1 TPGS:SOL weight ratio and a 2:3 OLA:RAPA molar ratio showed synergistic effects and were optimized. The drug encapsulation efficiency of this formulation was >65%, and the physicochemical properties were sustained for 180 days. Moreover, the formulation had a high cell uptake rate and significantly inhibited cell migration (**p < 0.01). In the in vivo toxicity test, no toxicity was observed, with the exception of the high dose group. Furthermore, OLA/RAPA-TPGS/SOL markedly inhibited tumor spheroid and tumor growth in vivo. Conclusion: Compared to the control, OLA/RAPA-TPGS/SOL showed significant tumor inhibition. These findings lay a foundation for the use of TPGS/SOL mixed micelles loaded with OLA and RAPA in the treatment of ovarian cancer.


Subject(s)
Micelles , Ovarian Neoplasms , Phthalazines , Piperazines , Polyethylene Glycols , Polyvinyls , Sirolimus , Vitamin E , Female , Ovarian Neoplasms/drug therapy , Ovarian Neoplasms/pathology , Piperazines/chemistry , Piperazines/pharmacology , Polyethylene Glycols/chemistry , Humans , Animals , Cell Line, Tumor , Vitamin E/chemistry , Vitamin E/pharmacology , Sirolimus/chemistry , Sirolimus/pharmacology , Sirolimus/administration & dosage , Sirolimus/pharmacokinetics , Phthalazines/chemistry , Phthalazines/pharmacology , Phthalazines/administration & dosage , Phthalazines/pharmacokinetics , Polyvinyls/chemistry , Polyvinyls/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Antineoplastic Agents/administration & dosage , Mice , Drug Carriers/chemistry , Xenograft Model Antitumor Assays , Mice, Nude , Mice, Inbred BALB C , Cell Survival/drug effects
4.
Molecules ; 29(15)2024 Jul 26.
Article in English | MEDLINE | ID: mdl-39124924

ABSTRACT

Active ingredients from Traditional Chinese Medicines (TCMs) have been a cornerstone of healthcare for millennia, offering a rich source of bioactive compounds with therapeutic potential. However, the clinical application of TCMs is often limited by challenges such as poor solubility, low bioavailability, and variable pharmacokinetics. To address these issues, the development of advanced polymer nanocarriers has emerged as a promising strategy for the delivery of TCMs. This review focuses on the introduction of common active ingredients from TCMs and the recent advancements in the design and application of polymer nanocarriers for enhancing the efficacy and safety of TCMs. We begin by discussing the unique properties of TCMs and the inherent challenges associated with their delivery. We then delve into the types of polymeric nanocarriers, including polymer micelles, polymer vesicles, polymer hydrogels, and polymer drug conjugates, highlighting their application in the delivery of active ingredients from TCMs. The main body of the review presents a comprehensive analysis of the state-of-the-art nanocarrier systems and introduces the impact of these nanocarriers on the solubility, stability, and bioavailability of TCM components. On the basis of this, we provide an outlook on the future directions of polymer nanocarriers in TCM delivery. This review underscores the transformative potential of polymer nanocarriers in revolutionizing TCM delivery, offering a pathway to harness the full therapeutic potential of TCMs while ensuring safety and efficacy in a modern medical context.


Subject(s)
Drug Carriers , Drugs, Chinese Herbal , Medicine, Chinese Traditional , Nanoparticles , Polymers , Polymers/chemistry , Drug Carriers/chemistry , Humans , Nanoparticles/chemistry , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/pharmacokinetics , Drug Delivery Systems , Animals , Biological Availability , Micelles
5.
Int J Mol Sci ; 25(15)2024 Aug 01.
Article in English | MEDLINE | ID: mdl-39125974

ABSTRACT

There is currently a growing interest in health-promoting foods. The beneficial effects of food on human health are actively promoted by health professionals and nutritionists. This growing awareness is influencing the increasing range of functional foods and the pursuit of more innovative solutions. Recent research indicates that spherical nanoparticles have the potential to be used as functional biomaterials in the food industry, particularly for encapsulating hydrophobic natural phytochemicals. Techniques and systems based on micro- and nano-encapsulation are of great importance in the food and pharmaceutical industries. It is of paramount importance that encapsulation materials are safe for use in food. The aim of this study was to obtain micelles containing extracts from chokeberry fruit pomace using egg yolk powder (EYP) for emulsification (as a source of lecithin) and egg white powder (EWP) for stabilisation. The structural properties of the micelles in the resulting powders were characterised using Fourier transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) analysis confirmed the presence of spherical micellar structures between 500 and 1000 nm in size. The water activity and water content of the obtained powders were determined, and the thermal (DSC) and antioxidant properties were investigated. The results indicated that the powder with the micellar structures had a higher stability compared to the powder obtained by simple mixing without the use of encapsulation techniques.


Subject(s)
Antioxidants , Egg White , Egg Yolk , Fruit , Micelles , Plant Extracts , Plant Extracts/chemistry , Egg Yolk/chemistry , Fruit/chemistry , Egg White/chemistry , Antioxidants/chemistry , Antioxidants/pharmacology , Spectroscopy, Fourier Transform Infrared , Nanoparticles/chemistry , Chemical Phenomena , Powders/chemistry
6.
Int J Mol Sci ; 25(15)2024 Aug 05.
Article in English | MEDLINE | ID: mdl-39126119

ABSTRACT

To achieve the optimal alginate-based oral formulation for delivery of hydrophobic drugs, on the basis of previous research, we further optimized the synthesis process parameters of alginate-g-oleylamine derivatives (Ugi-FOlT) and explored the effects of different degrees of substitution (DSs) on the molecular self-assembly properties of Ugi-FOlT, as well as the in vitro cytotoxicity and drug release behavior of Ugi-FOlT. The resultant Ugi-FOlT exhibited good amphiphilic properties with the critical micelle concentration (CMC) ranging from 0.043 mg/mL to 0.091 mg/mL, which decreased with the increase in the DS of Ugi-FOlT. Furthermore, Ugi-FOlT was able to self-assemble into spherical micellar aggregates in aqueous solution, whose sizes and zeta potentials with various DSs measured by dynamic light scattering (DLS) were in the range of 653 ± 25~710 ± 40 nm and -58.2 ± 1.92~-48.9 ± 2.86 mV, respectively. In addition, RAW 264.7 macrophages were used for MTT assay to evaluate the in vitro cytotoxicity of Ugi-FOlT in the range of 100~500 µg/mL, and the results indicated good cytocompatibility for Ugi-FOlT. Ugi-FOlT micellar aggregates with favorable stability also showed a certain sustained and pH-responsive release behavior for the hydrophobic drug ibuprofen (IBU). Meanwhile, it is feasible to control the drug release rate by regulating the DS of Ugi-FOlT. The influence of different DSs on the properties of Ugi-FOlT is helpful to fully understand the relationship between the micromolecular structure of Ugi-FOlT and its macroscopic properties.


Subject(s)
Alginates , Drug Liberation , Hydrophobic and Hydrophilic Interactions , Micelles , Alginates/chemistry , Mice , Animals , RAW 264.7 Cells , Amines/chemistry , Drug Delivery Systems , Drug Carriers/chemistry , Ibuprofen/chemistry , Ibuprofen/pharmacology , Cell Survival/drug effects
7.
J Environ Manage ; 367: 122069, 2024 Sep.
Article in English | MEDLINE | ID: mdl-39098071

ABSTRACT

Studying the adsorption behavior of cationic surfactants can help to develop more effective strategies to limit their dispersion in the environment. However, there have few studies on the adsorption of cationic surfactants from the perspective of critical micelle concentration (CMC). In this study, with cetyltrimethylammonium bromide (CTAB) and octadecyl trimethylammonium bromide (OTAB) serving as the model cationic surfactants, the effect of CMC on the adsorption behavior of cationic surfactant onto the surface of sodium alginate/silica (SA/SiO2) microspheres was systematically revealed. The adsorption mechanism relative to CMC was investigated under different conditions, including surfactant concentration, pH, temperature, and adsorption time. The results suggest that at identical concentrations, the smaller the CMC value of the cationic surfactants, the greater the adsorption amount (qt). qt for CTAB and OTAB were 583.2 and 678.0 mg/g respectively, with the concentration higher than their CMC value. When the concentration was lower than the CMC value of the cationic surfactants, qt for CTAB and OTAB were 123.2 and 138.7 mg/g, respectively. The CMC value of CTAB was lower than that of OTAB under identical conditions, suggesting that the adsorption of cationic surfactants is related to their CMC. These results are beneficial for the removal of cationic surfactants by adsorption methods.


Subject(s)
Cations , Micelles , Microspheres , Silicon Dioxide , Surface-Active Agents , Surface-Active Agents/chemistry , Adsorption , Silicon Dioxide/chemistry , Cations/chemistry , Cetrimonium/chemistry , Cetrimonium Compounds/chemistry , Alginates/chemistry , Hydrogen-Ion Concentration
8.
ACS Nano ; 18(28): 18604-18621, 2024 Jul 16.
Article in English | MEDLINE | ID: mdl-38952130

ABSTRACT

Chemo-immunotherapy has become a promising strategy for cancer treatment. However, the inability of the drugs to penetrate deeply into the tumor and form potent tumor vaccines in vivo severely restricts the antitumor effect of chemo-immunotherapy. In this work, an injectable sodium alginate platform is reported to promote penetration of the chemotherapeutic doxorubicin (DOX) and delivery of personalized tumor vaccines. The injectable multifunctional sodium alginate platform cross-links rapidly in the presence of physiological concentrations of Ca2+, forming a hydrogel that acts as a drug depot and releases loaded hyaluronidase (HAase), DOX, and micelles (IP-NPs) slowly and sustainedly. By degrading hyaluronic acid (HA) overexpressed in tumor tissue, HAase can make tumor tissue "loose" and favor other components to penetrate deeply. DOX induces potent immunogenic cell death (ICD) and produces tumor-associated antigens (TAAs), which could be effectively captured by polyethylenimine (PEI) coated IP-NPs micelles and form personalized tumor vaccines. The vaccines efficaciously facilitate the maturation of dendritic cells (DCs) and activation of T lymphocytes, thus producing long-term immune memory. Imiquimod (IMQ) loaded in the core could further activate the immune system and trigger a more robust antitumor immune effect. Hence, the research proposes a multifunctional drug delivery platform for the effective treatment of colorectal cancer.


Subject(s)
Alginates , Doxorubicin , Hydrogels , Immunotherapy , Nanoparticles , Alginates/chemistry , Hydrogels/chemistry , Animals , Nanoparticles/chemistry , Mice , Doxorubicin/chemistry , Doxorubicin/pharmacology , Humans , Cancer Vaccines/chemistry , Cancer Vaccines/administration & dosage , Hyaluronoglucosaminidase/metabolism , Micelles , Cell Line, Tumor
9.
Sci Transl Med ; 16(754): eadq6489, 2024 Jul 03.
Article in English | MEDLINE | ID: mdl-38959325

ABSTRACT

Nasal delivery of an oligomeric tau antibody loaded into micelles reduces pathology and ameliorates cognition in a mouse model of tauopathy.


Subject(s)
Administration, Intranasal , Tauopathies , tau Proteins , Animals , tau Proteins/metabolism , Mice , Tauopathies/metabolism , Tauopathies/pathology , Humans , Disease Models, Animal , Micelles , Nose , Antibodies/therapeutic use , Antibodies/immunology
10.
J Agric Food Chem ; 72(29): 16438-16448, 2024 Jul 24.
Article in English | MEDLINE | ID: mdl-38981019

ABSTRACT

Steviol glycosides (SGs) are a natural sweetener widely used in the food and beverage industry, but the low solubility and stability of SG aqueous solutions greatly limit their application performance, especially in liquid formulations. In this work, we explore the solubility behavior of rebaudioside A (Reb A) in water, a major component of SGs, with the aim of clarifying the underlying mechanisms of the solubility and stability constraints of SGs, as well as the impact on their multifunctional properties. We demonstrate for the first time that Reb A exhibits hierarchical self-assembly in solutions, forming spherical micelles first when the concentration exceeds its critical micelle concentration (5.071 mg/mL), which then further assemble into large rod-like aggregates. The formation of such large Reb A aggregates is mainly dominated by hydrogen bonding and short-range Coulomb interaction energy, thus leading to the low solubility and precipitation of Reb A solutions. Surprisingly, aggregated Reb A structures display significantly improved organoleptic properties, revealing that self-aggregation can be developed as a simple, efficient, and green strategy for improving the taste profile of SGs. Additionally, the self-aggregation of Reb A at high concentrations impairs active encapsulation and also affects its interfacial and emulsifying properties.


Subject(s)
Diterpenes, Kaurane , Glycosides , Solubility , Sweetening Agents , Diterpenes, Kaurane/chemistry , Sweetening Agents/chemistry , Glycosides/chemistry , Water/chemistry , Micelles , Hydrogen Bonding , Taste , Glucosides/chemistry , Stevia/chemistry , Solutions/chemistry
11.
Langmuir ; 40(29): 14908-14921, 2024 Jul 23.
Article in English | MEDLINE | ID: mdl-39001842

ABSTRACT

It is crucial to use simple methods to prepare stable polymeric micelles with multiple functions for cancer treatment. Herein, via a "bottom-up" strategy, we reported the fabrication of ß-CD-(PEOSMA-PCPTMA-PPEGMA)21 (ßPECP) unimolecular micelles that could simultaneously treat tumors and bacteria with chemotherapy and photodynamic therapy (PDT). The unimolecular micelles consisted of a 21-arm ß-cyclodextrin (ß-CD) core as a macromolecular initiator, photosensitizer eosin Y (EOS-Y) monomer EOSMA, anticancer drug camptothecin (CPT) monomer, and a hydrophilic shell PEGMA. Camptothecin monomer (CPTMA) could achieve controlled release of the CPT due to the presence of responsively broken disulfide bonds. PEGMA enhanced the biocompatibility of micelles as a hydrophilic shell. Two ßPECP with different lengths were synthesized by modulating reaction conditions and the proportion of monomers, which both were self-assembled to unimolecular micelles in water. ßPECP unimolecular micelles with higher EOS-Y/CPT content exhibited more excellent 1O2 production, in vitro drug release efficiency, higher cytotoxicity, and superior antibacterial activity. Also, we carried out simulations of the self-assembly and CPT release process of micelles, which agreed with the experiments. This nanosystem, which combines antimicrobial and antitumor functions, provides new ideas for bacteria-mediated tumor clinical chemoresistance.


Subject(s)
Antineoplastic Agents , Micelles , Photochemotherapy , Photosensitizing Agents , Photosensitizing Agents/chemistry , Photosensitizing Agents/pharmacology , Humans , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Prodrugs/chemistry , Prodrugs/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Animals , Mice , beta-Cyclodextrins/chemistry , Camptothecin/chemistry , Camptothecin/pharmacology
12.
Langmuir ; 40(29): 15293-15300, 2024 Jul 23.
Article in English | MEDLINE | ID: mdl-39007240

ABSTRACT

Controlling physicochemical processes that drive changes in supramolecular aggregates is an important objective toward creating artificial soft micro- and nanomachines. Previous research explored the morphology control of membrane-based materials subjected to externally imposed chemical stimuli. Here, we modulate the microscale morphology of pH-responsive assemblies by using biocatalysis to internally generate changes in global pH. Catalytic reactions offer flexibility in the mechanism and rate at which stimuli are introduced to responsive assemblies, ultimately enabling precision and control over size and morphology. We observed, by dynamic light scattering and fluorescence microscopy, substantial microscale differences between assemblies subjected to manually titrated pH changes compared to biocatalytically activated pH changes, including the growth of giant vesicles from micelles. Coarse-grained molecular dynamics simulations of these metastable self-assembled structures provided insight into the thermodynamics and kinetics of the preferred structures. These results demonstrate the feasibility of using biocatalytic reactions to modulate the size and morphology of supramolecular assemblies, from micelles to giant vesicles.


Subject(s)
Biocatalysis , Micelles , Hydrogen-Ion Concentration , Molecular Dynamics Simulation , Lipase/chemistry , Lipase/metabolism , Kinetics , Thermodynamics
13.
J Nanobiotechnology ; 22(1): 419, 2024 Jul 16.
Article in English | MEDLINE | ID: mdl-39014410

ABSTRACT

BACKGROUND: Iron oxide nanoparticles (IONPs) have been cleared by the Food and Drug Administration (FDA) for various clinical applications, such as tumor-targeted imaging, hyperthermia therapy, drug delivery, and live-cell tracking. However, the application of IONPs as T1 contrast agents has been restricted due to their high r2 values and r2/r1 ratios, which limit their effectiveness in T1 contrast enhancement. Notably, IONPs with diameters smaller than 5 nm, referred to as extremely small-sized IONPs (ESIONs), have demonstrated potential in overcoming these limitations. To advance the clinical application of ESIONs as T1 contrast agents, we have refined a scale-up process for micelle encapsulation aimed at improving the hydrophilization of ESIONs, and have carried out comprehensive in vivo biodistribution and preclinical toxicity assessments. RESULTS: The optimization of the scale-up micelle-encapsulation process, specifically employing Tween60 at a concentration of 10% v/v, resulted in ESIONs that were uniformly hydrophilized, with an average size of 9.35 nm and a high purification yield. Stability tests showed that these ESIONs maintained consistent size over extended storage periods and dispersed effectively in blood and serum-mimicking environments. Relaxivity measurements indicated an r1 value of 3.43 mM- 1s- 1 and a favorable r2/r1 ratio of 5.36, suggesting their potential as T1 contrast agents. Biodistribution studies revealed that the ESIONs had extended circulation times in the bloodstream and were primarily cleared via the hepatobiliary route, with negligible renal excretion. We monitored blood clearance and organ distribution using positron emission tomography and magnetic resonance imaging (MRI). Additionally, MRI signal variations in a dose-dependent manner highlighted different behaviors at varying ESIONs concentrations, implying that optimal dosages might be specific to the intended imaging application. Preclinical safety evaluations indicated that ESIONs were tolerable in rats at doses up to 25 mg/kg. CONCLUSIONS: This study effectively optimized a scale-up process for the micelle encapsulation of ESIONs, leading to the production of hydrophilic ESIONs at gram-scale levels. These optimized ESIONs showcased properties conducive to T1 contrast imaging, such as elevated r1 relaxivity and a reduced r2/r1 ratio. Biodistribution study underscored their prolonged bloodstream presence and efficient clearance through the liver and bile, without significant renal involvement. The preclinical toxicity tests affirmed the safety of the ESIONs, supporting their potential use as T1 contrast agent with versatile clinical application.


Subject(s)
Contrast Media , Magnetic Iron Oxide Nanoparticles , Magnetic Resonance Imaging , Micelles , Particle Size , Animals , Contrast Media/chemistry , Contrast Media/pharmacokinetics , Tissue Distribution , Magnetic Resonance Imaging/methods , Magnetic Iron Oxide Nanoparticles/chemistry , Magnetic Iron Oxide Nanoparticles/toxicity , Mice , Rats , Male , Humans , Female
14.
J Nanobiotechnology ; 22(1): 420, 2024 Jul 16.
Article in English | MEDLINE | ID: mdl-39014462

ABSTRACT

Triple negative breast cancer (TNBC) has the characteristics of low immune cell infiltration, high expression of tumor programmed death ligand 1 (PD-L1), and abundant cancer stem cells. Systemic toxicity of traditional chemotherapy drugs due to poor drug selectivity, and chemotherapy failure due to tumor drug resistance and other problems, so it is particularly important to find new cancer treatment strategies for TNBC with limited treatment options. Both the anti-tumor natural drugs curcumin and ginsenoside Rg3 can exert anti-tumor effects by inducing immunogenic cell death (ICD) of tumor cells, reducing PD-L1 expression, and reducing cancer stem cells. However, they have the disadvantages of poor water solubility, low bioavailability, and weak anti-tumor effect of single agents. We used vinyl ether bonds to link curcumin (Cur) with N-O type zwitterionic polymers and at the same time encapsulated ginsenoside Rg3 to obtain hyperbranched zwitterionic drug-loaded micelles OPDEA-PGED-5HA@Cur@Rg3 (PPH@CR) with pH response. In vitro cell experiments and in vivo animal experiments have proved that PPH@CR could not only promote the maturation of dendritic cells (DCs) and increase the CD4+ T cells and CD8+ T cells by inducing ICD in tumor cells but also reduce the expression of PD-L1 in tumor tissues, and reduce cancer stem cells and showed better anti-tumor effects and good biological safety compared with free double drugs, which is a promising cancer treatment strategy.


Subject(s)
Antineoplastic Agents , B7-H1 Antigen , Curcumin , Ginsenosides , Animals , Curcumin/pharmacology , Curcumin/chemistry , Ginsenosides/chemistry , Ginsenosides/pharmacology , Humans , Hydrogen-Ion Concentration , Mice , Antineoplastic Agents/pharmacology , Antineoplastic Agents/chemistry , Cell Line, Tumor , Female , B7-H1 Antigen/metabolism , Triple Negative Breast Neoplasms/drug therapy , Micelles , Mice, Inbred BALB C , Polymers/chemistry , Polymers/pharmacology , Dendritic Cells/drug effects , Nanoparticles/chemistry , Neoplastic Stem Cells/drug effects , Drug Carriers/chemistry , Oxides/chemistry , Oxides/pharmacology
15.
Int J Mol Sci ; 25(14)2024 Jul 10.
Article in English | MEDLINE | ID: mdl-39062820

ABSTRACT

Colorectal cancer (CRC) is the third most prominent cancer worldwide, and the second leading cause of cancer death. Poor outcomes and limitations of current treatments fuel the search for new therapeutic options. Curcumin (CUR) is often presented as a safer alternative for cancer treatment with a staggering number of molecular targets involved in tumor initiation, promotion, and progression. Despite being promising, its therapeutic potential is hindered due to its hydrophobic nature. Hence, the ongoing development of optimal delivery strategies based on nanotechnology, such as polymeric micelles (PMs), to overcome issues in CUR solubilization and delivery to tumor cells. In this sense, this study aimed to optimize the development and stability of CUR-loaded P123:F127:TPGS PMs (PFT:CUR) based on the thin-film approach and evaluate their therapeutic potential in CRC. Overall, the results revealed that the solubility of CUR was improved when room temperature was used to hydrate the film. The PFT-CUR hydrated at room temperature presents an average hydrodynamic diameter of 15.9 ± 0.3 nm with a polydispersity index (PDI) of 0.251 ± 0.103 and a zeta potential of -1.5 ± 1.9 mV, and a 35.083 ± 1.144 encapsulation efficiency (EE%) and 3.217 ± 0.091 drug loading (DL%) were observed. To ensure the stability of the optimized PFT-CUR nanosystems, different lyophilization protocols were tested, the use of 1% of glycine (GLY) being the most promising protocol. Regarding the critical micellar concentration (CMC), it was shown that the cryoprotectant and the lyophilization process could impact it, with an increase from 0.064 mg/mL to 0.119 mg/mL. In vitro results showed greater cytotoxic effects when CUR was encapsulated compared to its free form, yet further analysis revealed the heightened cytotoxicity could be attributed to the system itself. Despite challenges, the developed CUR-loaded PM shows potential as an effective therapeutic agent for CRC. Nonetheless, the system must undergo refinements to enhance drug entrapment as well as improve overall stability.


Subject(s)
Colorectal Neoplasms , Curcumin , Micelles , Vitamin E , Curcumin/chemistry , Curcumin/pharmacology , Colorectal Neoplasms/drug therapy , Colorectal Neoplasms/pathology , Humans , Vitamin E/chemistry , Drug Carriers/chemistry , Poloxalene/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Cell Survival/drug effects , Solubility , Polymers/chemistry , Drug Liberation
16.
Biomaterials ; 311: 122708, 2024 Dec.
Article in English | MEDLINE | ID: mdl-39047538

ABSTRACT

The resistance of glioblastoma multiforme (GBM) to standard chemotherapy is primarily attributed to the existence of tumor-associated macrophages (TAMs) in the GBM microenvironment, particularly the anti-inflammatory M2 phenotype. Targeted modulation of M2-TAMs is emerging as a promising strategy to enhance chemotherapeutic efficacy. However, combination TAM-targeted therapy with chemotherapy faces substantial challenges, notably in terms of delivery efficiency and targeting specificity. In this study, we designed a pH-responsive hierarchical brain-targeting micelleplex loaded with temozolomide (TMZ) and resiquimod (R848) for combination chemo-immunotherapy against GBM. This delivery system, termed PCPA&PPM@TR, features a primary Angiopep-2 decoration on the outer layer via a pH-cleavable linker and a secondary mannose analogue (MAN) on the middle layer. This pH-responsive hierarchical targeting strategy enables effective BBB permeability while simultaneous GBM- and TAMs-targeting delivery. GBM-targeted delivery of TMZ induces alkylation and triggers an anti-GBM immune response. Concurrently, TAM-targeted delivery of R848 reprograms their phenotype from M2 to pro-inflammatory M1, thereby diminishing GBM resistance to TMZ and amplifying the immune response. In vivo studies demonstrated that targeted modulation of TAMs using PCPA&PPM@TR significantly enhanced anti-GBM efficacy. In summary, this study proposes a promising brain-targeting delivery system for the targeted modulation of TAMs to combat GBM.


Subject(s)
Drug Resistance, Neoplasm , Glioblastoma , Immunotherapy , Temozolomide , Tumor-Associated Macrophages , Glioblastoma/drug therapy , Glioblastoma/pathology , Glioblastoma/therapy , Tumor-Associated Macrophages/drug effects , Tumor-Associated Macrophages/immunology , Tumor-Associated Macrophages/metabolism , Temozolomide/pharmacology , Temozolomide/therapeutic use , Animals , Humans , Drug Resistance, Neoplasm/drug effects , Immunotherapy/methods , Cell Line, Tumor , Brain Neoplasms/drug therapy , Brain Neoplasms/pathology , Brain Neoplasms/therapy , Mice , Imidazoles/pharmacology , Imidazoles/chemistry , Drug Delivery Systems , Cellular Reprogramming/drug effects , Micelles , Tumor Microenvironment/drug effects , Hydrogen-Ion Concentration
17.
Drug Discov Today ; 29(8): 104098, 2024 Aug.
Article in English | MEDLINE | ID: mdl-38997002

ABSTRACT

Block copolymer micelles, formed by the self-assembly of amphiphilic polymers, address formulation challenges, such as poor drug solubility and permeability. These micelles offer advantages including a smaller size, easier preparation, sterilization, and superior solubilization, compared with other nanocarriers. Preclinical studies have shown promising results, advancing them toward clinical trials. Their mucoadhesive properties enhance and prolong contact with the ocular surface, and their small size allows deeper penetration through tissues, such as the cornea. Additionally, copolymeric micelles improve the solubility and stability of hydrophobic drugs, sustain drug release, and allow for surface modifications to enhance biocompatibility. Despite these benefits, long-term stability remains a challenge. In this review, we highlight the preclinical performance, structural frameworks, preparation techniques, physicochemical properties, current developments, and prospects of block copolymer micelles as ocular drug delivery systems.


Subject(s)
Administration, Ophthalmic , Drug Delivery Systems , Micelles , Polymers , Humans , Drug Delivery Systems/methods , Polymers/chemistry , Animals , Drug Carriers/chemistry , Solubility
18.
Sci Rep ; 14(1): 16588, 2024 07 18.
Article in English | MEDLINE | ID: mdl-39025925

ABSTRACT

Invasive fungal infections (IFI) pose a significant health burden, leading to high morbidity, mortality, and treatment costs. This study aims to develop and characterize nanomicelles for the codelivery of posaconazole and hemp seed oil for IFI via the oral route. The nanomicelles were prepared using a nanoprecipitation method and optimized through the Box Behnken design. The optimized nanomicelles resulted in satisfactory results for zeta potential, size, PDI, entrapment efficiency, TEM, and stability studies. FTIR and DSC results confirm the compatibility and amorphous state of the prepared nanomicelles. Confocal laser scanning microscopy showed that the optimized nanomicelles penetrated the tissue more deeply (44.9µm) than the suspension (25µm). The drug-loaded nanomicelles exhibited sustained cumulative drug release of 95.48 ± 3.27% for 24 h. The nanomicelles showed significant inhibition against Aspergillus niger and Candida albicans (22.4 ± 0.21 and 32.2 ± 0.46 mm, respectively). The pharmacokinetic study on Wistar rats exhibited a 1.8-fold increase in relative bioavailability for the nanomicelles compared to the suspension. These results confirm their therapeutic efficacy and lay the groundwork for future research and clinical applications, providing a promising synergistic antifungal nanomicelles approach for treating IFIs.


Subject(s)
Antifungal Agents , Plant Oils , Animals , Antifungal Agents/administration & dosage , Antifungal Agents/pharmacokinetics , Antifungal Agents/pharmacology , Antifungal Agents/chemistry , Rats , Plant Oils/chemistry , Plant Oils/pharmacology , Plant Oils/administration & dosage , Triazoles/administration & dosage , Triazoles/pharmacokinetics , Triazoles/chemistry , Triazoles/pharmacology , Nanoparticles/chemistry , Rats, Wistar , Candida albicans/drug effects , Invasive Fungal Infections/drug therapy , Aspergillus niger/drug effects , Micelles , Seeds/chemistry , Drug Liberation , Male , Drug Carriers/chemistry
19.
Nanomedicine (Lond) ; 19(14): 1297-1311, 2024.
Article in English | MEDLINE | ID: mdl-39046514

ABSTRACT

Aim: To develop a robust drug-delivery system using multi-arm amphiphilic block copolymers for enhanced efficacy in cancer therapy. Materials & methods: Two series of amphiphilic polymer micelles, PEG-b-PCLm and PEG-b-PCLm/TPGS, were synthesized. Doxorubicin (DOX) loading into the micelles was achieved via solvent dialysis. Results: The micelles displayed excellent biocompatibility, narrow size distribution, and uniform morphology. DOX-loaded micelles exhibited enhanced antitumor efficacy and increased drug accumulation at tumor sites compared with free DOX. Additionally, 4A-PEG47-b-PCL21/TPGS micelles effectively suppressed drug-resistant MCF-7/ADR cells. Conclusion: This study introduces a novel micelle formulation with exceptional serum stability and efficacy against drug resistance, promising for cancer therapy. It highlights innovative strategies for refining clinical translation and ensuring sustained efficacy and safety in vivo.


[Box: see text].


Subject(s)
Doxorubicin , Drug Resistance, Neoplasm , Micelles , Polyethylene Glycols , Doxorubicin/pharmacology , Doxorubicin/chemistry , Humans , Drug Resistance, Neoplasm/drug effects , Polyethylene Glycols/chemistry , Animals , MCF-7 Cells , Drug Carriers/chemistry , Mice , Vitamin E/chemistry , Vitamin E/pharmacology , Female , Mice, Inbred BALB C , Polymers/chemistry , Mice, Nude , Antibiotics, Antineoplastic/pharmacology , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/administration & dosage , Polyesters/chemistry , Drug Delivery Systems , Cell Survival/drug effects
20.
Biosensors (Basel) ; 14(7)2024 Jul 11.
Article in English | MEDLINE | ID: mdl-39056614

ABSTRACT

Terahertz spectroscopy has unique advantages in the study of biological molecules in aqueous solutions. However, water has a strong absorption capability in the terahertz region. Reducing the amount of liquid could decrease interference with the terahertz wave, which may, however, affect the measurement accuracy. Therefore, it is particularly important to balance the amount and water content of liquid samples. In this work, a terahertz metamaterial sensor based on metallic strips is designed, fabricated, and used to detect reverse micelles. An aqueous confinement environment in reverse micelles can improve the signal-to-noise ratio of the terahertz response. Due to "water pool" trapped in reverse micelles, the DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) solution and DOPC emulsion can successfully be identified in intensity by terahertz spectroscopy. Combined with the metamaterial sensor, an obvious frequency shift of 30 GHz can be achieved to distinguish the DOPC emulsion (5%) from the DOPC solution. This approach may provide a potential way for improving the sensitivity of detecting trace elements in a buffer solution, thus offering a valuable toolkit toward bioanalytical applications.


Subject(s)
Micelles , Terahertz Spectroscopy , Biosensing Techniques , Metals/chemistry , Phosphatidylcholines/chemistry , Water/chemistry
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