ABSTRACT
Canine Leproid Granuloma Syndrome (CLGS), also known as canine leprosy, is a cutaneous nodular infectious disease caused by Mycobacterium sp.. Despite being reported worldwide, it is still quite unknown and underdiagnosed. Diagnosis may be achieved by cytopathology or histopathology of skin lesions, but identification of the infectious agent is complex, since bacterial in vitro growth is not possible, relying upon molecular techniques such as PCR to confirm Mycobacterium DNA in the sample. We report a CLGS case in Niteroi, Rio de Janeiro state, Brazil, diagnosed by cytopathology and submitted to molecular identification of the agent. PCR amplification of hsp65 gene was performed and revealed 100% genetic homology to M. murphy strain. This is the first CLGS report with molecular identification in Rio de Janeiro state, and this finding should raise awareness about CLGS as a differential diagnosis among granulomatous skin diseases in this region.(AU)
A síndrome de granuloma leproide canino (SGLC), também conhecida como lepra canina, é uma doença infecciosa cutânea nodular causada por Mycobacterium sp. Apesar de ser relatada mundialmente, ainda é bastante desconhecida e subdiagnosticada. O diagnóstico pode ser conseguido por citopatologia ou histopatologia de lesões cutâneas, mas a identificação do agente infeccioso é complexa, uma vez que o crescimento in vitro bacteriano não é possível, dependendo de técnicas moleculares como a PCR para confirmar o DNA de Mycobacterium na amostra. Relatou-se um caso da SGLC em Niterói, estado do Rio de Janeiro, Brasil, diagnosticado por citopatologia e submetido à identificação molecular do agente. Foi realizada amplificação por PCR do gene hsp65, que revelou 100% de homologia genética com a cepa M. murphy. Este é o primeiro relato da SGLC com identificação molecular no estado do Rio de Janeiro, o que mostra a importância de se acrescentar a SGLC ao diagnóstico diferencial das doenças granulomatosas de pele nessa região.(AU)
Subject(s)
Animals , Dogs , Polymerase Chain Reaction/statistics & numerical data , Mycobacterium/cytology , Mycobacterium/pathogenicity , Mycobacterium Infections , DogsABSTRACT
Canine Leproid Granuloma Syndrome (CLGS), also known as canine leprosy, is a cutaneous nodular infectious disease caused by Mycobacterium sp.. Despite being reported worldwide, it is still quite unknown and underdiagnosed. Diagnosis may be achieved by cytopathology or histopathology of skin lesions, but identification of the infectious agent is complex, since bacterial in vitro growth is not possible, relying upon molecular techniques such as PCR to confirm Mycobacterium DNA in the sample. We report a CLGS case in Niteroi, Rio de Janeiro state, Brazil, diagnosed by cytopathology and submitted to molecular identification of the agent. PCR amplification of hsp65 gene was performed and revealed 100% genetic homology to M. murphy strain. This is the first CLGS report with molecular identification in Rio de Janeiro state, and this finding should raise awareness about CLGS as a differential diagnosis among granulomatous skin diseases in this region.(AU)
A síndrome de granuloma leproide canino (SGLC), também conhecida como lepra canina, é uma doença infecciosa cutânea nodular causada por Mycobacterium sp. Apesar de ser relatada mundialmente, ainda é bastante desconhecida e subdiagnosticada. O diagnóstico pode ser conseguido por citopatologia ou histopatologia de lesões cutâneas, mas a identificação do agente infeccioso é complexa, uma vez que o crescimento in vitro bacteriano não é possível, dependendo de técnicas moleculares como a PCR para confirmar o DNA de Mycobacterium na amostra. Relatou-se um caso da SGLC em Niterói, estado do Rio de Janeiro, Brasil, diagnosticado por citopatologia e submetido à identificação molecular do agente. Foi realizada amplificação por PCR do gene hsp65, que revelou 100% de homologia genética com a cepa M. murphy. Este é o primeiro relato da SGLC com identificação molecular no estado do Rio de Janeiro, o que mostra a importância de se acrescentar a SGLC ao diagnóstico diferencial das doenças granulomatosas de pele nessa região.(AU)
Subject(s)
Animals , Dogs , Polymerase Chain Reaction , Mycobacterium/cytology , Mycobacterium/pathogenicity , Mycobacterium Infections , DogsABSTRACT
Os testes bioquímicos realizados, o seqüenciamento de diferentes alvos genéticos e a construção de uma árvore concatenada, construída a través do método Neighbor-Joining, permitiram a identificação das cepas brasileiras como M. kyorinense.
Subject(s)
Brazil , Nontuberculous Mycobacteria/isolation & purification , Nontuberculous Mycobacteria/virology , Mycobacterium/isolation & purification , Mycobacterium/cytology , Mycobacterium/virologyABSTRACT
Os testes bioquímicos realizados, o seq³enciamento de diferentes alvos genéticos e a construþÒo de uma árvore concatenada, construída a través do método Neighbor-Joining, permitiram a identificaþÒo das cepas brasileiras como M. kyorinense. (AU)
Subject(s)
Mycobacterium/cytology , Mycobacterium/isolation & purification , Mycobacterium/virology , Nontuberculous Mycobacteria/isolation & purification , Nontuberculous Mycobacteria/virology , BrazilABSTRACT
Three recently isolated strains of a fast gowing non-pigmented mycobacterium, two of which were from a series of injection abscesses and one which was recovered from a water tank, have been compared bacteriologically and serologically in gel-diffusion tests with a number of mycobacterial type strains. The three strains and type strains of M. abscessus, M. runyonii, M. borstelense and M. friedmannii were of one species. This species deiffered in several respects from M. ranae (fortuitum), M. smegmatis and M. thamnopheos. In view of the chronological priority of M. friedmannii over the other names, we recommend that this name be adopted as the correct desigantion for this species. The origin of the type strains of M. friedmannii is discussed and reasons are given for its acceptance as representative of Friedmann´s original strains.
Subject(s)
Amidohydrolases/analysis , Carbohydrates/metabolism , Immunodiffusion , Mycobacterium/cytology , Mycobacterium/classification , Mycobacterium/enzymology , Mycobacterium/metabolism , Drug Resistance, Microbial , SerotypingABSTRACT
One hundred and thirty-three strains of acid-fast organism have been isolated from 234 specimens of water, beach-sand and mud from dams and creeks. Stagnant and semistagnant water such as swimming pools, reservoirs and rain water tanks are the richest source of mycobacteria in the surroundings of man. The percentage of positive isolations and the number of strains which are able to produce skin lesions and sensitize guinea pigs to Old Tuberculin seem to be greater in North Queensland and Brisbane than in Sydney. This parallels the conversion rates in children of 10-14 years which are around 50%, 25% and 5% respectively