ABSTRACT
In chordates, the central nervous system arises from precursors that have distinct developmental and transcriptional trajectories. Anterior nervous systems are ontogenically associated with ectodermal lineages while posterior nervous systems are associated with mesoderm. Taking advantage of the well-documented cell lineage of ascidian embryos, we asked to what extent the transcriptional states of the different neural lineages become similar during the course of progressive lineage restriction. We performed single-cell RNA sequencing (scRNA-seq) analyses on hand-dissected neural precursor cells of the two distinct lineages, together with those of their sister cell lineages, with a high temporal resolution covering five successive cell cycles from the 16-cell to neural plate stages. A transcription factor binding site enrichment analysis of neural specific genes at the neural plate stage revealed limited evidence for shared transcriptional control between the two neural lineages, consistent with their different ontogenies. Nevertheless, PCA analysis and hierarchical clustering showed that, by neural plate stages, the two neural lineages cluster together. Consistent with this, we identified a set of genes enriched in both neural lineages at the neural plate stage, including miR-124, Celf3.a, Zic.r-b, and Ets1/2. Altogether, the current study has revealed genome-wide transcriptional dynamics of neural progenitor cells of two distinct developmental origins. Our scRNA-seq dataset is unique and provides a valuable resource for future analyses, enabling a precise temporal resolution of cell types not previously described from dissociated embryos.
Subject(s)
Cell Lineage , Embryonic Development , Gene Expression Regulation, Developmental , Animals , Cell Lineage/genetics , Embryonic Development/genetics , Neural Plate/embryology , Neural Plate/metabolism , Neural Plate/cytology , Ciona intestinalis/embryology , Ciona intestinalis/genetics , Urochordata/embryology , Urochordata/genetics , Single-Cell Analysis , Embryo, Nonmammalian/metabolism , Embryo, Nonmammalian/cytology , Transcription Factors/metabolism , Transcription Factors/genetics , Neural Stem Cells/metabolism , Neural Stem Cells/cytologyABSTRACT
The development of the human central nervous system initiates in the early embryonic period until long after delivery. It has been shown that several neurological and neuropsychiatric diseases originate from prenatal incidents. Mathematical models offer a direct way to understand neurodevelopmental processes better. Mathematical modelling of neurodevelopment during the embryonic period is challenging in terms of how to 'Approach', how to initiate modelling and how to propose the appropriate equations that fit the underlying dynamics of neurodevelopment during the embryonic period while including the variety of elements that are built-in naturally during the process of neurodevelopment. It is imperative to answer where and how to start modelling; in other words, what is the appropriate 'Approach'? Therefore, one objective of this study was to tackle the mathematical issue broadly from different aspects and approaches. The approaches were divided into three embryonic categories: cell division, neural tube growth and neural plate growth. We concluded that the neural plate growth approach provides a suitable platform for simulation of brain formation/neurodevelopment compared to cell division and neural tube growth. We devised a novel equation and designed algorithms that include geometrical and topological algorithms that could fit most of the necessary elements of the neurodevelopmental process during the embryonic period. Hence, the proposed equations and defined mathematical structure would be a platform to generate an artificial neural network that autonomously grows and develops.
Subject(s)
Models, Biological , Neural Tube , Animals , Humans , Algorithms , Cell Division , Embryonic Development , Models, Neurological , Neural Networks, Computer , Neural Plate/cytology , Neural Plate/embryology , Neural Tube/embryology , Neurogenesis , Neurons/cytologyABSTRACT
During the formation of the neural tube, the primordium of the vertebrate central nervous system, the actomyosin activity of cells in different regions drives neural plate bending. However, how the stiffness of the neural plate and surrounding tissues is regulated and mechanically influences neural plate bending has not been elucidated. Here, we used atomic force microscopy to reveal the relationship between the stiffness of the neural plate and the mesoderm during Xenopus neural tube formation. Measurements with intact embryos revealed that the stiffness of the neural plate was consistently higher compared with the non-neural ectoderm and that it increased in an actomyosin activity-dependent manner during neural plate bending. Interestingly, measurements of isolated tissue explants also revealed that the relationship between the stiffness of the apical and basal sides of the neural plate was reversed during bending and that the stiffness of the mesoderm was lower than that of the basal side of the neural plate. The experimental elevation of mesoderm stiffness delayed neural plate bending, suggesting that low mesoderm stiffness mechanically supports neural tube closure. This study provides an example of mechanical interactions between tissues during large-scale morphogenetic movements.
Subject(s)
Neural Plate , Neural Tube , Xenopus laevis , Animals , Neural Tube/embryology , Neural Tube/cytology , Neural Tube/metabolism , Neural Plate/embryology , Neural Plate/metabolism , Neural Plate/cytology , Xenopus laevis/embryology , Mesoderm/cytology , Mesoderm/embryology , Mesoderm/metabolism , Ectoderm/cytology , Ectoderm/metabolism , Microscopy, Atomic Force , Embryo, Nonmammalian/cytology , Embryo, Nonmammalian/metabolism , Embryo, Nonmammalian/embryologyABSTRACT
Neural crest cells exemplify cellular diversification from a multipotent progenitor population. However, the full sequence of early molecular choices orchestrating the emergence of neural crest heterogeneity from the embryonic ectoderm remains elusive. Gene-regulatory-networks (GRN) govern early development and cell specification toward definitive neural crest. Here, we combine ultradense single-cell transcriptomes with machine-learning and large-scale transcriptomic and epigenomic experimental validation of selected trajectories, to provide the general principles and highlight specific features of the GRN underlying neural crest fate diversification from induction to early migration stages using Xenopus frog embryos as a model. During gastrulation, a transient neural border zone state precedes the choice between neural crest and placodes which includes multiple converging gene programs. During neurulation, transcription factor connectome, and bifurcation analyses demonstrate the early emergence of neural crest fates at the neural plate stage, alongside an unbiased multipotent-like lineage persisting until epithelial-mesenchymal transition stage. We also decipher circuits driving cranial and vagal neural crest formation and provide a broadly applicable high-throughput validation strategy for investigating single-cell transcriptomes in vertebrate GRNs in development, evolution, and disease.
Subject(s)
Neural Crest , Single-Cell Analysis , Xenopus laevis , Animals , Neural Crest/cytology , Neural Crest/metabolism , Single-Cell Analysis/methods , Xenopus laevis/embryology , Gene Expression Regulation, Developmental , Cell Movement , Gene Regulatory Networks , Transcriptome , Gastrulation , Neural Plate/metabolism , Neural Plate/embryology , Neural Plate/cytology , Epithelial-Mesenchymal Transition/genetics , Embryo, Nonmammalian/metabolism , Embryo, Nonmammalian/cytology , Neurulation/genetics , Neurulation/physiology , Cell DifferentiationABSTRACT
Understanding human organ formation is a scientific challenge with far-reaching medical implications1,2. Three-dimensional stem-cell cultures have provided insights into human cell differentiation3,4. However, current approaches use scaffold-free stem-cell aggregates, which develop non-reproducible tissue shapes and variable cell-fate patterns. This limits their capacity to recapitulate organ formation. Here we present a chip-based culture system that enables self-organization of micropatterned stem cells into precise three-dimensional cell-fate patterns and organ shapes. We use this system to recreate neural tube folding from human stem cells in a dish. Upon neural induction5,6, neural ectoderm folds into a millimetre-long neural tube covered with non-neural ectoderm. Folding occurs at 90% fidelity, and anatomically resembles the developing human neural tube. We find that neural and non-neural ectoderm are necessary and sufficient for folding morphogenesis. We identify two mechanisms drive folding: (1) apical contraction of neural ectoderm, and (2) basal adhesion mediated via extracellular matrix synthesis by non-neural ectoderm. Targeting these two mechanisms using drugs leads to morphological defects similar to neural tube defects. Finally, we show that neural tissue width determines neural tube shape, suggesting that morphology along the anterior-posterior axis depends on neural ectoderm geometry in addition to molecular gradients7. Our approach provides a new route to the study of human organ morphogenesis in health and disease.
Subject(s)
Morphogenesis , Neural Tube/anatomy & histology , Neural Tube/embryology , Organ Culture Techniques/methods , Ectoderm/cytology , Ectoderm/embryology , Humans , Models, Biological , Neural Plate/cytology , Neural Plate/embryology , Neural Tube/cytology , Neural Tube Defects/embryology , Neural Tube Defects/pathology , Regeneration , Stem Cells/cytologyABSTRACT
The Ciona larva has served as a unique model for understanding the development of dopaminergic cells at single-cell resolution owing to the exceptionally small number of neurons in its brain and its fixed cell lineage during embryogenesis. A recent study suggested that the transcription factors Fer2 and Meis directly regulate the dopamine synthesis genes in Ciona, but the dopaminergic cell lineage and the gene regulatory networks that control the development of dopaminergic cells have not been fully elucidated. Here, we reveal that the dopaminergic cells in Ciona are derived from a bilateral pair of cells called a9.37 cells at the center of the neural plate. The a9.37 cells divide along the anterior-posterior axis, and all of the descendants of the posterior daughter cells differentiate into the dopaminergic cells. We show that the MAPK pathway and the transcription factor Otx are required for the expression of Fer2 in the dopaminergic cell lineage. Our findings establish the cellular and molecular framework for fully understanding the commitment to dopaminergic cells in the simple chordate brain.
Subject(s)
Brain/cytology , Brain/metabolism , Cell Differentiation/genetics , Ciona/genetics , Dopaminergic Neurons/metabolism , Mitogen-Activated Protein Kinases/genetics , Otx Transcription Factors/genetics , Animals , Biomarkers , Cell Lineage/genetics , Ciona/cytology , Dopaminergic Neurons/cytology , Fluorescent Antibody Technique , Gene Expression , Gene Expression Regulation, Developmental , Gene Regulatory Networks , Mitogen-Activated Protein Kinases/metabolism , Neural Plate/cytology , Neural Plate/metabolism , Otx Transcription Factors/metabolism , Signal TransductionABSTRACT
Morphogenesis of the vertebrate neural tube occurs by elongation and bending of the neural plate, tissue shape changes that are driven at the cellular level by polarized cell intercalation and cell shape changes, notably apical constriction and cell wedging. Coordinated cell intercalation, apical constriction, and wedging undoubtedly require complex underlying cytoskeletal dynamics and remodeling of adhesions. Mutations of the gene encoding Scribble result in neural tube defects in mice, however the cellular and molecular mechanisms by which Scrib regulates neural cell behavior remain unknown. Analysis of Scribble mutants revealed defects in neural tissue shape changes, and live cell imaging of mouse embryos showed that the Scrib mutation results in defects in polarized cell intercalation, particularly in rosette resolution, and failure of both cell apical constriction and cell wedging. Scrib mutant embryos displayed aberrant expression of the junctional proteins ZO-1, Par3, Par6, E- and N-cadherins, and the cytoskeletal proteins actin and myosin. These findings show that Scribble has a central role in organizing the molecular complexes regulating the morphomechanical neural cell behaviors underlying vertebrate neurulation, and they advance our understanding of the molecular mechanisms involved in mammalian neural tube closure.
Subject(s)
Intracellular Signaling Peptides and Proteins/genetics , Neural Tube Defects/embryology , Neural Tube/embryology , Animals , Cell Polarity , Cell Shape , Cytoskeletal Proteins , Gene Expression , Intercellular Junctions/metabolism , Intercellular Junctions/ultrastructure , Intracellular Signaling Peptides and Proteins/metabolism , Mice , Morphogenesis , Mutation , Nerve Tissue Proteins/genetics , Neural Plate/cytology , Neural Plate/embryology , Neural Tube/cytology , Neural Tube Defects/genetics , Neuroepithelial Cells/cytology , Neuroepithelial Cells/metabolism , Neuroepithelial Cells/ultrastructure , Tight Junction Proteins/genetics , Tight Junction Proteins/metabolismABSTRACT
Alternative splicing (AS) is involved in cell fate decisions and embryonic development. However, regulation of these processes is poorly understood. Here, we have identified the serine threonine kinase receptor-associated protein (STRAP) as a putative spliceosome-associated factor. Upon Strap deletion, there are numerous AS events observed in mouse embryoid bodies (EBs) undergoing a neuroectoderm-like state. Global mapping of STRAP-RNA binding in mouse embryos by enhanced-CLIP sequencing (eCLIP-seq) reveals that STRAP preferably targets transcripts for nervous system development and regulates AS through preferred binding positions, as demonstrated for two neuronal-specific genes, Nnat and Mark3. We have found that STRAP involves in the assembly of 17S U2 snRNP proteins. Moreover, in Xenopus, loss of Strap leads to impeded lineage differentiation in embryos, delayed neural tube closure, and altered exon skipping. Collectively, our findings reveal a previously unknown function of STRAP in mediating the splicing networks of lineage commitment, alteration of which may be involved in early embryonic lethality in mice.
Subject(s)
Alternative Splicing , Cell Differentiation/genetics , Mouse Embryonic Stem Cells/cytology , RNA-Binding Proteins/metabolism , Animals , Cell Lineage/genetics , Embryo, Mammalian , Embryo, Nonmammalian , Embryonic Development/genetics , Exons , Mice , Mouse Embryonic Stem Cells/metabolism , Neural Plate/cytology , Organogenesis/genetics , Protein Binding , RNA, Messenger/metabolism , RNA-Binding Proteins/genetics , Ribonucleoprotein, U2 Small Nuclear/metabolism , Spliceosomes/metabolism , Xenopus laevisABSTRACT
This essay represents a critical analysis of the literary data on various types of waves occurring in the amphibian embryos during gastrulation. A surface contraction wave travels through the presumptive neurectoderm during Mexican axolotl gastrulation. This wave coincides temporally and spatially with involution of the inducing chordomesoderm and with the prospective neural plate. By contrast, there is no similar surface contraction wave during African clawed frog gastrulation. However, the clawed frog displays the waves of DNA synthesis and mitosis in the presumptive neurectoderm during gastrulation, whereas no such waves were discovered in axolotl gastrulae. These sets of experimental data are in accordance with the contemporary concept of considerable ontogenetic diversity of the class Amphibia.
Subject(s)
Cell Differentiation/physiology , Cell Proliferation/physiology , Gastrula/physiology , Gastrulation/physiology , Neural Plate/physiology , Ambystoma mexicanum , Animals , Cell Differentiation/genetics , Cell Proliferation/genetics , DNA Replication/genetics , DNA Replication/physiology , Gastrula/cytology , Gastrulation/genetics , Mitosis/genetics , Mitosis/physiology , Neural Plate/cytology , Species Specificity , Xenopus laevisABSTRACT
Melanocytes are neuroectoderm-derived pigment-producing cells with highly polarized dendritic morphology. They protect the skin against ultraviolet radiation by providing melanin to neighbouring keratinocytes. However, the mechanisms underlying melanocyte polarization and its relevance for diseases remain mostly elusive. Numerous studies have instead revealed roles for polarity regulators in other neuroectoderm-derived lineages including different neuronal cell types. Considering the shared ontogeny and morphological similarities, these lineages may be used as reference models for the exploration of melanocyte polarity, for example, regarding dendrite formation, spine morphogenesis and polarized organelle transport. In this review, we summarize and compare the latest progress in understanding polarity regulation in neuronal cells and melanocytes and project key open questions for future work.
Subject(s)
Cell Differentiation , Cell Lineage , Cell Polarity , Melanocytes/cytology , Neural Plate/cytology , Humans , Keratinocytes/cytology , Keratinocytes/metabolism , Melanocytes/metabolism , Melanosomes/metabolismABSTRACT
Neural crest cells (NCCs) contribute to several tissues during embryonic development. NCC formation depends on activation of tightly regulated molecular programs at the neural plate border (NPB) region, which initiate NCC specification and epithelial-to-mesenchymal transition (EMT). Although several approaches to investigate NCCs have been devised, these early events of NCC formation remain largely unknown in humans, and currently available cellular models have not investigated EMT. Here, we report that the E6 neural induction protocol converts human induced pluripotent stem cells into NPB-like cells (NBCs), from which NCCs can be efficiently derived. NBC-to-NCC induction recapitulates gene expression dynamics associated with NCC specification and EMT, including downregulation of NPB factors and upregulation of NCC specifiers, coupled with other EMT-associated cell-state changes, such as cadherin modulation and activation of TWIST1 and other EMT inducers. This strategy will be useful in future basic or translational research focusing on these early steps of NCC formation.
Subject(s)
Epithelial-Mesenchymal Transition , Neural Crest/cytology , Neural Plate/cytology , Cell Line , Humans , Multipotent Stem Cells/cytology , Twist-Related Protein 1/metabolism , Up-RegulationABSTRACT
BACKGROUND: Diverse architectures of nervous systems (NSs) such as a plexus in cnidarians or a more centralized nervous system (CNS) in insects and vertebrates are present across Metazoa, but it is unclear what selection pressures drove evolution and diversification of NSs. One underlying aspect of this diversity lies in the cellular and molecular mechanisms driving neurogenesis, i.e. generation of neurons from neural precursor cells (NPCs). In cnidarians, vertebrates, and arthropods, homologs of SoxB and bHLH proneural genes control different steps of neurogenesis, suggesting that some neurogenic mechanisms may be conserved. However, data are lacking for spiralian taxa. RESULTS: To that end, we characterized NPCs and their daughters at different stages of neurogenesis in the spiralian annelid Capitella teleta. We assessed cellular division patterns in the neuroectoderm using static and pulse-chase labeling with thymidine analogs (EdU and BrdU), which enabled identification of NPCs that underwent multiple rounds of division. Actively-dividing brain NPCs were found to be apically-localized, whereas actively-dividing NPCs for the ventral nerve cord (VNC) were found apically, basally, and closer to the ventral midline. We used lineage tracing to characterize the changing boundary of the trunk neuroectoderm. Finally, to start to generate a genetic hierarchy, we performed double-fluorescent in-situ hybridization (FISH) and single-FISH plus EdU labeling for neurogenic gene homologs. In the brain and VNC, Ct-soxB1 and Ct-neurogenin were expressed in a large proportion of apically-localized, EdU+ NPCs. In contrast, Ct-ash1 was expressed in a small subset of apically-localized, EdU+ NPCs and subsurface, EdU- cells, but not in Ct-neuroD+ or Ct-elav1+ cells, which also were subsurface. CONCLUSIONS: Our data suggest a putative genetic hierarchy with Ct-soxB1 and Ct-neurogenin at the top, followed by Ct-ash1, then Ct-neuroD, and finally Ct-elav1. Comparison of our data with that from Platynereis dumerilii revealed expression of neurogenin homologs in proliferating NPCs in annelids, which appears different than the expression of vertebrate neurogenin homologs in cells that are exiting the cell cycle. Furthermore, differences between neurogenesis in the head versus trunk of C. teleta suggest that these two tissues may be independent developmental modules, possibly with differing evolutionary trajectories.
Subject(s)
Neurogenesis/genetics , Phylogeny , Polychaeta/cytology , Polychaeta/genetics , Animals , Brain/cytology , Cell Cycle/genetics , Cell Division , Cell Proliferation/genetics , Gene Expression Regulation , Gene Regulatory Networks , Kinetics , Models, Biological , Neural Plate/cytology , Neural Stem Cells/cytology , Neural Stem Cells/metabolism , SOX Transcription Factors/metabolismABSTRACT
The expression and biological functions of oncofetal markers GD2 and GD3 were extensively studied in neuroectoderm-derived cancers in order to characterize their potential as therapeutic targets. Using immunological approaches, we previously identified GD3, GD2, and OAcGD2 expression in breast cancer (BC) cell lines. However, antibodies specific for O-acetylated gangliosides are not exempt of limitations, as they only provide information on the expression of a limited set of O-acetylated ganglioside species. Consequently, the aim of the present study was to use structural approaches in order to apprehend ganglioside diversity in melanoma, neuroblastoma, and breast cancer cells, focusing on O-acetylated species that are usually lost under alkaline conditions and require specific analytical procedures. We used purification and extraction methods that preserve the O-acetyl modification for the analysis of native gangliosides by MALDI-TOF. We identified the expression of GM1, GM2, GM3, GD2, GD3, GT2, and GT3 in SK-Mel28 (melanoma), LAN-1 (neuroblastoma), Hs 578T, SUM 159PT, MDA-MB-231, MCF-7 (BC), and BC cell lines over-expressing GD3 synthase. Among O-acetylated gangliosides, we characterized the expression of OAcGM1, OAcGD3, OAcGD2, OAcGT2, and OAcGT3. Furthermore, the experimental procedure allowed us to clearly identify the position of the sialic acid residue that carries the O-acetyl group on b- and c-series gangliosides by MS/MS fragmentation. These results show that ganglioside O-acetylation occurs on both inner and terminal sialic acid residue in a cell type-dependent manner, suggesting different O-acetylation pathways for gangliosides. They also highlight the limitation of immuno-detection for the complete identification of O-acetylated ganglioside profiles in cancer cells.
Subject(s)
Acetyltransferases/metabolism , Gangliosides/metabolism , Neural Plate/cytology , Acetylation , Acetyltransferases/genetics , Breast Neoplasms/metabolism , Female , Gangliosides/chemistry , Humans , MCF-7 Cells , Melanoma/metabolism , N-Acetylneuraminic Acid/chemistry , N-Acetylneuraminic Acid/metabolism , Neural Plate/metabolism , Neuroblastoma/metabolismABSTRACT
The embryonic development of the pineal organ, a neuroendocrine gland on top of the diencephalon, remains enigmatic. Classic fate-mapping studies suggested that pineal progenitors originate from the lateral border of the anterior neural plate. We show here, using gene expression and fate mapping/lineage tracing in zebrafish, that pineal progenitors originate, at least in part, from the non-neural ectoderm. Gene expression in chick indicates that this non-neural origin of pineal progenitors is conserved in amniotes. Genetic repression of placodal, but not neural crest, cell fate results in pineal hypoplasia in zebrafish, while mis-expression of transcription factors known to specify placodal identity during gastrulation promotes the formation of ectopic pineal progenitors. We also demonstrate that fibroblast growth factors (FGFs) position the pineal progenitor domain within the non-neural border by repressing pineal fate and that the Otx transcription factors promote pinealogenesis by inhibiting this FGF activity. The non-neural origin of the pineal organ reveals an underlying similarity in the formation of the pineal and pituitary glands, and suggests that all CNS neuroendocrine organs may require a non-neural contribution to form neurosecretory cells.
Subject(s)
Fibroblast Growth Factors/metabolism , Pineal Gland/cytology , Pineal Gland/embryology , Signal Transduction , Zebrafish/embryology , Animals , Animals, Genetically Modified , Cell Lineage , Chick Embryo , Ectoderm/cytology , Gastrulation , Gene Expression Regulation, Developmental , Green Fluorescent Proteins/metabolism , Neural Crest/cytology , Neural Plate/cytology , Neuroglia/cytology , Neurons/cytology , Neurosecretory Systems/metabolism , Transcription Factors/metabolism , Zebrafish Proteins/metabolismABSTRACT
Genetic lineage-tracing techniques are powerful tools for studying specific cell populations in development and pathogenesis. Previous techniques have mainly involved systems for tracing a single gene, which are limited in their ability to facilitate direct comparisons of the contributions of different cell lineages. We have developed a new combinatorial system for tracing all three germ layers using self-cleaving 2A peptides and multiple site-specific recombinases (SSRs). In the resulting TRiCK (TRiple Coloured germ layer Knock-in) mice, the three germ layers are conditionally and simultaneously labelled with distinct fluorescent proteins via embryogenesis. We show that previously reported ectopic expressions of lineage markers are the outcome of secondary gene expression. The results presented here also indicate that the commitment of caudal axial stem cells to neural or mesodermal fate proceeds without lineage fluctuations, contrary to the notion of their bi-potency. Moreover, we developed IMES, an optimized tissue clearing method that is highly compatible with a variety of fluorescent proteins and immunostaining, and the combined use of TRiCK mice and IMES can facilitate comprehensive analyses of dynamic contributions of all three germ layers.
Subject(s)
Cell Lineage , Gene Expression Regulation, Developmental , Gene Knock-In Techniques , Germ Layers/cytology , Animals , Brain/metabolism , Crosses, Genetic , DNA Nucleotidyltransferases/metabolism , Embryonic Stem Cells/cytology , Endoderm/cytology , Endothelium, Vascular/cytology , Female , Genotype , Green Fluorescent Proteins/metabolism , HEK293 Cells , Heart/embryology , Humans , Imaging, Three-Dimensional , Liver/embryology , Male , Mesoderm/cytology , Mice , Mice, Inbred C57BL , Myocardium/cytology , Neural Plate/cytologyABSTRACT
A definitive endodermal cell lineage is a prerequisite for the efficient generation of mature endoderm derivatives that give rise to organs, such as the pancreas and liver. We previously reported that the induction of mesenchymal definitive endoderm cells depends on autocrine TGF-ß signaling and that pharmacological blockage of TGF-ß signaling by Repsox disrupts endoderm specification. The definitive endoderm arises from a primitive streak, which depends largely on TGF-ß signaling. If the TGF-ß pathway is blocked by Repsox, cell fate after the primitive streak induction is so-far unknown. We report here, that an induced primitive streak cell-population contained many T/SOX2 co-expressing cells, and subsequent inhibition of TGF-ß signaling by Repsox promoted neuroectodermal cell fate, which was characterized using single-cell qPCR analysis and immunostaining. The process of epithelial-to-mesenchymal transition, which is inherent to the process of definitive endoderm differentiation, was also disrupted upon Repsox treatment. Our findings may provide a new approach to produce neural progenitors.
Subject(s)
Cell Lineage/drug effects , Endoderm/cytology , Epithelial-Mesenchymal Transition/drug effects , Neural Plate/cytology , Polymerase Chain Reaction , Pyrazoles/pharmacology , Pyridines/pharmacology , Single-Cell Analysis , Activins/pharmacology , Humans , Primitive Streak/cytology , Signal Transduction/drug effects , Transforming Growth Factor beta/metabolismABSTRACT
SOX2 and OCT4 are pioneer transcription factors playing a key role in embryonic stem (ES) cell self-renewal and differentiation. How temporal fluctuations in their expression levels bias lineage commitment is unknown. Here, we generated knock-in reporter fusion ES cell lines allowing to monitor endogenous SOX2 and OCT4 protein fluctuations in living cells and to determine their impact on mesendodermal and neuroectodermal commitment. We found that small differences in SOX2 and OCT4 levels impact cell fate commitment in G1 but not in S phase. Elevated SOX2 levels modestly increased neuroectodermal commitment and decreased mesendodermal commitment upon directed differentiation. In contrast, elevated OCT4 levels strongly biased ES cells towards both neuroectodermal and mesendodermal fates in undirected differentiation. Using ATAC-seq on ES cells gated for different endogenous SOX2 and OCT4 levels, we found that high OCT4 levels increased chromatin accessibility at differentiation-associated enhancers. This suggests that small endogenous fluctuations of pioneer transcription factors can bias cell fate decisions by concentration-dependent priming of differentiation-associated enhancers.
Subject(s)
Cell Differentiation/genetics , Octamer Transcription Factor-3 , Pluripotent Stem Cells/physiology , SOXB1 Transcription Factors , Animals , Cell Line , Endoderm/cytology , Endoderm/metabolism , Enhancer Elements, Genetic/genetics , Gene Knock-In Techniques/methods , Mice , Neural Plate/cytology , Neural Plate/metabolism , Octamer Transcription Factor-3/genetics , Octamer Transcription Factor-3/metabolism , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , SOXB1 Transcription Factors/genetics , SOXB1 Transcription Factors/metabolismABSTRACT
Mouse embryonic stem cells (mESCs) are pluripotent cells capable of differentiating in vitro to form the ~200 types of cells of the developing embryo and adult, including cells of the nervous system. This makes mESCs a useful tool for studying the molecular mechanisms of mammalian embryonic development. Many protocols involving the use of growth factors and small molecules to differentiate mESCs into neural progenitors and neurons currently exist. However, there is a paucity of protocols available that recapitulate the developmental process. Our laboratory has developed a protocol to recapitulate mammalian neural lineage development by differentiating mESCs to mature neurons via intermediate cell populations observed during in vivo embryo development. This protocol uses the amino acid L-proline to direct the differentiation of mESCs, grown as embryoid bodies, into Sox1+ neurectoderm, followed by differentiation to form Nestin+, BLBP+, and NeuN+ neural cell types.
Subject(s)
Cell Differentiation/physiology , Mouse Embryonic Stem Cells/cytology , Neural Plate/cytology , Neurons/cytology , Stem Cells/cytology , Animals , Cell Line , Embryoid Bodies/cytology , Embryonic Development/physiology , Mammals/physiology , Mice , Nervous System/cytology , Neurogenesis/physiologyABSTRACT
Gain of 20q11.21 is one of the most common recurrent genomic aberrations in human pluripotent stem cells. Although it is known that overexpression of the antiapoptotic gene Bcl-xL confers a survival advantage to the abnormal cells, their differentiation capacity has not been fully investigated. RNA sequencing of mutant and control hESC lines, and a line transgenically overexpressing Bcl-xL, shows that overexpression of Bcl-xL is sufficient to cause most transcriptional changes induced by the gain of 20q11.21. Moreover, the differentially expressed genes in mutant and Bcl-xL overexpressing lines are enriched for genes involved in TGF-ß- and SMAD-mediated signaling, and neuron differentiation. Finally, we show that this altered signaling has a dramatic negative effect on neuroectodermal differentiation, while the cells maintain their ability to differentiate to mesendoderm derivatives. These findings stress the importance of thorough genetic testing of the lines before their use in research or the clinic.
Subject(s)
Cell Differentiation/genetics , Chromosomes, Human, Pair 20/genetics , Pluripotent Stem Cells/cytology , Transforming Growth Factor beta/metabolism , Chromosome Aberrations , Chromosomes, Human, Pair 20/chemistry , DNA-Binding Proteins/genetics , Down-Regulation , Gene Amplification , Humans , Neural Plate/cytology , Pluripotent Stem Cells/metabolism , Sequence Analysis, RNA , Signal Transduction , Smad Proteins/genetics , Smad Proteins/metabolism , Transcription Factors/genetics , Transforming Growth Factor beta/genetics , bcl-X Protein/genetics , bcl-X Protein/metabolismABSTRACT
How pluripotent stem cells differentiate into the main germ layers is a key question of developmental biology. Here, we show that the chromatin-related factor Whsc1 (also known as Nsd2 and MMSET) has a dual role in pluripotency exit and germ layer specification of embryonic stem cells. On induction of differentiation, a proportion of Whsc1-depleted embryonic stem cells remain entrapped in a pluripotent state and fail to form mesendoderm, although they are still capable of generating neuroectoderm. These functions of Whsc1 are independent of its methyltransferase activity. Whsc1 binds to enhancers of the mesendodermal regulators Gata4, T (Brachyury), Gata6 and Foxa2, together with Brd4, and activates the expression of these genes. Depleting each of these regulators also delays pluripotency exit, suggesting that they mediate the effects observed with Whsc1. Our data indicate that Whsc1 links silencing of the pluripotency regulatory network with activation of mesendoderm lineages.